Review
Food Science & Technology
Tianyi Guo, Otobong Donald Akan, Feijun Luo, Qinlu Lin
Summary: Polysaccharides, derived from natural products, are valued for their effective health-related activities. Recent studies have shown that polysaccharides exert biological effects through epigenetic mechanisms, participating in the regulation of immunity, inflammation, metabolism, and antioxidant damage. This review comprehensively summarizes and discusses the latest advances in the biological functions of dietary polysaccharides via epigenetic regulations.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Genetics & Heredity
Lili Jiang, Zunhe Du, Guizhi Zhang, Teng Wang, Guanghui Jin
Summary: This article introduces the mechanisms and progress of using RNA interference technology to enhance potato's resistance to viruses, and discusses the challenges in overcoming RNAi.
Article
Marine & Freshwater Biology
Gregory P. Toth, David C. Bencic, John W. Martinson, Robert W. Flick, David L. Lattier, Mitchell S. Kostich, Weichun Huang, Adam D. Biales
Summary: This study aimed to develop novel methodologies to expand the panel of gene expression-based biomarkers, including responses to estrogens, and to evaluate the potential of small non-coding RNAs as biomarkers. The research findings showed that miRNA and piRNA could respond to estrogen exposure, indicating the need for further investigation into their regulatory roles in the estrogenic response.
AQUATIC TOXICOLOGY
(2021)
Article
Cell Biology
Tian-Jing Yang, Mu-Di Yao, Ya-Nan Sun, Xiu-Miao Li, Qin Jiang, Biao Yan
Summary: In this study, IPW was found to play a role in the progression of choroidal neovascularization (CNV) and could be a potential target for the treatment of neovascular ocular diseases. Silencing IPW led to reduced CNV formation and altered endothelial cell angiogenic phenotypes through upregulation of miR-370. These findings suggest that IPW silencing may be a promising therapeutic strategy for neovascular ocular diseases.
Article
Medicine, Research & Experimental
Loredana Migliore, Federico Galvagni, Enrico Pierantozzi, Vincenzo Sorrentino, Daniela Rossi
Summary: This study investigates the use of ASP-RNAi as a strategy to silence mutant alleles in TNNT2, a sarcomere protein associated with hypertrophic and dilated cardiomyopathy. Through experimental validation, different siRNAs were shown to effectively knockdown the mutant alleles. This research provides a foundation for the application of allele silencing as a therapeutic treatment for TNNT2-mutation-associated cardiomyopathies.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Courtney F. Jungers, Sergej Djuranovic
Summary: This review summarizes our current knowledge on gene expression regulation through interactions of miRNAs and RBPs.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Ma'mon M. Hatmal, Mohammad A. I. Al-Hatamleh, Amin N. Olaimat, Walhan Alshaer, Hanan Hasan, Khaled A. Albakri, Enas Alkhafaji, Nada N. Issa, Murad A. Al-Holy, Salim M. Abderrahman, Atiyeh M. Abdallah, Rohimah Mohamud
Summary: Human breast milk contains not only nutritional components but also abundant microRNAs, which can impact epigenetic mechanisms and regulate immune and physiological functions. Studying the microRNA composition of human breast milk is important for improving nutrition during pregnancy and infancy and preventing future diseases.
Article
Microbiology
Edoardo Piombo, Bekele Gelena Kelbessa, Poorva Sundararajan, Stephen C. Whisson, Ramesh Raju Vetukuri, Mukesh Dubey
Summary: In this study, the genomes of several oomycete species were analyzed to investigate the role of RNA silencing. It was found that RNA silencing plays a crucial role in limiting the expansion of transposons, regulating gene expression, and potentially controlling infection in oomycetes. Novel miRNA-like RNAs (milRNAs) were also discovered, which may have implications in pathogenicity and biocontrol.
FRONTIERS IN MICROBIOLOGY
(2023)
Letter
Oncology
Shrikant B. Mali
Summary: Epigenetic events, including DNA methylation, chromatin remodeling, histone posttranslational covalent modifications, and non-coding RNA effects, play an important role in the development and progression of head and neck cancer. Better understanding of aberrant patterns of methylation may provide biomarkers for early detection and diagnosis, while knowledge about target genes of microRNAs may improve the therapy of head and neck cancer and extend overall survival.
Review
Biochemistry & Molecular Biology
Anna Bogaczyk, Izabela Zawlik, Tomasz Zuzak, Marta Kluz, Natalia Potocka, Tomasz Kluz
Summary: Endometrial cancer is a common cancer with a high detection rate, but lacks markers for disease monitoring. MiRNAs have emerged as biomarkers in many cancers due to their role in regulating gene expression. This paper discusses the selected miRNAs and their impact on the development of endometrial cancer based on existing literature.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Wei Zhu, Xiangming Xiao, Jinqin Chen
Summary: In epithelial ovarian cancer (EOC), the overexpression of LINC01132 is significantly associated with poor patient prognosis. LINC01132 exhibits oncogenic activities in EOC cells by regulating the miR-431-5p/SOX9 axis, influencing the malignant behaviors of EOC cells in vitro and in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Kenneth Wu, Namrita Dhillon, Kelvin Du, Rohinton T. Kamakaka
Summary: Gene silencing in budding yeast is maintained by unacetylated nucleosomes and requires 50% to 75% acetylation for activation. The Sir4 gene is a key component in maintaining gene silencing, with even a two-fold reduction in acetyltransferases potentially leading to transcriptional silence.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Xingxing Peng, Xiaonan Ma, Shuting Lu, Zhenghe Li
Summary: A multipurpose plant rhabdovirus-based vector system has been developed for a wide range of applications in Nicotiana benthamiana, allowing for gene silencing, overexpression, and expression of antibodies. This system highlights the utility of plant virus vectors in gene function studies and agricultural biotechnology, providing a technical template for developing similar vectors of other economically important plant rhabdoviruses.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Pharmacology & Pharmacy
A. Dushani C. U. Ranasinghe, Margaret A. Schwarz
Summary: This article summarizes the emerging epigenetic mechanisms, including DNA methylation, histone modifications, and microRNAs, that contribute to the evolution of pulmonary arterial hypertension (PAH). The interplay between epigenetics and metabolism is explored and new crosstalk targets for investigation in PAH are proposed. The article also highlights the therapeutic opportunities and challenges associated with epigenetics and metabolomics in PAH, including early detection, personalized dietary plans, and advanced drug therapy.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Qiuxia Hao, Qingzhang Xu, Shuyan Niu, Caifeng Ding, Xiliang Luo
Summary: An ECL microRNA biosensor was developed using anti-fouling MBs and two signal amplification strategies. The designed anti-fouling dendritic peptide on MBs resisted nonspecific adsorption in biological samples for accurate target recognition. The biosensor demonstrated ultra-high sensitivity and excellent selectivity for miRNA 21 detection, with a detection limit of 0.13 fM, and was capable of assaying targets in complex biological media such as serum and cell lysate.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Bo Wang, Fengge Zhang, Jinlu Hu, Xiang Gao, Po Bian, Yongding Liu, Gaohong Wang
JOURNAL OF PLANT PHYSIOLOGY
(2019)
Article
Biology
Chenguang Deng, Jingjing Wu, Ting Wang, Gaohong Wang, Lijun Wu, Yuejin Wu, Po Bian
RADIATION RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Jun Sha, Zhiying Lu, Jing Ye, Gaohong Wang, Qiang Hu, Yongsheng Chen, Xuezhi Zhang
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2019)
Article
Ecology
Achal Rastogi, Fabio Rocha Jimenez Vieira, Anne-Flore Deton-Cabanillas, Alaguraj Veluchamy, Catherine Cantrel, Gaohong Wang, Pieter Vanormelingen, Chris Bowler, Gwenael Piganeau, Hanhua Hu, Leila Tirichine
Article
Genetics & Heredity
Huangqi Tang, Liangwen Chen, Zhangyu Dai, Wenjing Zhang, Ting Wang, Lijun Wu, Gaohong Wang, Po Bian
Article
Soil Science
Songqiang Deng, Dayi Zhang, Gaohong Wang, Xiangjun Zhou, Chaoran Ye, Taoran Fu, Tan Ke, Yurui Zhang, Yongding Liu, Lanzhou Chen
SOIL BIOLOGY & BIOCHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Zhiying Lu, Jun Sha, Wenxuan Wang, Yanhua Li, Gaohong Wang, Yongsheng Chen, Qiang Hu, Xuezhi Zhang
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2019)
Article
Cell Biology
Ru Jia, Yongping Chai, Chao Xie, Gai Liu, Zhiwen Zhu, Kaiyao Huang, Wei Li, Guangshuo Ou
JOURNAL OF CELL SCIENCE
(2020)
Article
Astronomy & Astrophysics
Bo Wang, Tong Ye, Xiaoyan Li, Po Bian, Yongding Liu, Gaohong Wang
Summary: The study exposed dried cells of desert green algae Chlorella to Mars-like near-space environment, revealing survival strategies and damages incurred. Results suggest extremophiles may potentially colonize the surface of Mars and other extraterrestrial habitats.
LIFE SCIENCES IN SPACE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Tong Ye, Bo Wang, Caiyan Li, Po Bian, Lanzhou Chen, Gaohong Wang
Summary: The study found that Nostoc sp. cells have the potential to survive in a Mars-like environment with low mortality rate and minimal physiological changes. RNA-sequencing data revealed several affected metabolic pathways. Ground exposed samples showed a similar trend to flight-exposed samples.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Environmental Sciences
Bo Wang, Tong Ye, Caiyan Li, Xiaoyan Li, Lanzhou Chen, Gaohong Wang
Summary: In this study, it was found that desert green algae, Chlorella sp., can maintain high photosynthetic activity under UV-B stress. The results showed that Chlorella sp. may have strong system characteristics for scavenging reactive oxygen species (ROS) and the antioxidant enzyme system exhibited a protective effect against enhanced UV-B radiation. Transcriptomic data revealed that a strong photosynthetic system, efficient DNA repair, and changes in the expression of genes encoding ribosomal protein are responsible for the high UV-B tolerance characteristics of Chlorella sp.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Yixiao Zhang, Zixu Chen, Xiaoyan Li, Xinguo Wu, Lanzhou Chen, Gaohong Wang
Summary: With the development of agriculture and the widespread application of agrichemicals, herbicide residues in Tibetan water bodies have become a threat to their ecological safety. This study investigated the inhibitory effect of glyphosate, a herbicide, on Tibetan algae in order to evaluate ecological health and protect the Tibetan water ecosystems. The results showed that high concentrations of glyphosate significantly inhibited photosynthetic activity and reduced pigment content in the algae, indicating a potential decrease in the primary production of aquatic ecosystems.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Article
Microbiology
Caiyan Li, Xianyuan Zhang, Tong Ye, Xiaoyan Li, Gaohong Wang
Summary: This study investigated the survival and endurance mechanisms of the desert cyanobacterium Chroococcidiopsis sp. in a Mars-like near space environment. It was found that UV radiation was the most important limiting factor for its survival, followed by temperature. The activation of genes involved in extracellular polysaccharides, carotenoid, scytonemin, and protein syntheses provided additional protection to the cells. The repair mechanisms played a vital role in the recovery of Chroococcidiopsis sp. after the flight. This study enhances our understanding of the tolerance mechanism of Chroococcidiopsis sp. and provides important guidance for the survival requirements of microbial life in a Mars-like environment.
MICROBIOLOGY SPECTRUM
(2022)
Article
Plant Sciences
Hui Chen, Qing-Lin Yang, Jia-Xi Xu, Xuan Deng, Yun-Jie Zhang, Ting Liu, Marianne G. Rots, Guo-Liang Xu, Kai-Yao Huang
Summary: In this study, a multi-type and precise genetic manipulation method was developed for efficient gene editing in Chlamydomonas. The method involved generating a DNA break using Cas9 nuclease and repairing it using a homologous DNA template. Various gene editing techniques were successfully demonstrated, including gene inactivation, epitope tag insertion, amino acid substitution, and fragment deletion. The study highlights the potential of Chlamydomonas as a model organism for both basic research and industrial applications.
Article
Plant Sciences
Huan Long, Jinhua Fang, Lian Ye, Baolong Zhang, Colleen Hui, Xuan Deng, Sabeeha S. Merchant, Kaiyao Huang
Summary: This study optimized a method for purifying lysosome-related organelles (LROs) from two different populations of cells and discovered differences in morphology and function between them. It found that LROs in stationary phase cells had non-membrane-bound polyphosphate granules for phosphorus storage, while those in iron-overloaded cells were similar to acidocalcisomes with membrane boundaries and larger polyphosphate granules. Additionally, the study established a method for quantifying the sequestration capacity of LROs for trace metals and identified 113 putative acidocalcisome proteins through comparative proteomic analysis.