Article
Biochemistry & Molecular Biology
Shidong Zhao, Wenwen Ru, Xinyan Chen, Shanhui Liao, Zhongliang Zhu, Jiahai Zhang, Chao Xu
Summary: C-degrons are critical for targeting receptor proteins of Cullin-RING E3 ligase complexes for protein degradation. FEM1 proteins, including FEM1B, recognize specific Arg/C-degrons such as CDK5R1 and SMCR8. The study reveals the binding mechanism between FEM1B and SMCR8, as well as the role of CRL2(FEM1B) in regulating the lifespan of SMCR8.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jiaojiao Bai, Guozhong Huang, Kexin Chen, Ranran Wang, Dongping Lu
Summary: RLCK subfamily VII members play important roles in immune signaling regulation; in the RLCK VII-4 subgroup, some members are under proteasomal control, with PCRK2 degradation dependent on its kinase activity, while plasma membrane localization is not required for degradation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Han Yong Lee, Hye Lin Park, Chanung Park, Yi-Chun Chen, Gyeong Mee Yoon
Summary: The study reveals a mechanism by which plants respond to stress by modulating the homeostasis of ACS and its cognate E3 ligases, maintaining protein stability and influencing stress responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Jiayi Yu, Lu Kang, Yuanyuan Li, Changai Wu, Chengchao Zheng, Pei Liu, Jinguang Huang
Summary: This study identified that RGLG1 and RGLG2 negatively regulate the drought stress tolerance mediated by MAPKKK18 in Arabidopsis by promoting the degradation of MAPKKK18 through ubiquitination. Deletion of RGLG1 and RGLG2 stabilizes MAPKKK18 and enhances drought stress tolerance in MAPKKK18-overexpression plants.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Review
Cell Biology
Albano Toska, Nikita Modi, Lin-Feng Chen
Summary: RUNX3 is a transcription factor involved in cell proliferation and development. It can act both as a tumor suppressor and an oncogene in different cancers. The tumor suppressor function of RUNX3 is mainly attributed to its ability to suppress cancer cell proliferation through ubiquitination and proteasomal degradation, while it can also be inactivated through the same mechanism.
Article
Plant Sciences
Shengyong Wang, Huili Chen, Yujie Huang, Xiaotian Zhang, Yuhang Chen, Hewei Du, Hongwei Wang, Feng Qin, Shuangcheng Ding
Summary: Cytoplasm-localized RING ubiquitin E3 ligase AtCHYR2 plays an important role in plant glucose responses.
PLANT CELL REPORTS
(2023)
Article
Plant Sciences
Jae Ho Kim, Moon Seok Kim, Yong Weon Seo
Summary: Drought stress can lead to significant reductions in crop production and yield. Plant U-box proteins (PUB) play a crucial role in response to abiotic stress. However, the specific role of TaPUB4, a gene encoding the U-box and nuclear localization domains, in wheat abiotic stress response remains unknown.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Laura Marquez-Cantudo, Ana Ramos, Claire Coderch, Beatriz de Pascual-Teresa
Summary: Protein degradation through the Ubiquitin-Proteasome System is a crucial mechanism for cellular proteostasis regulation, with E3 ligases playing a key role in protein recognition and degradation. Zinc-dependent HDACs are important in chromatin packing and gene expression regulation, making them targets for the design of HDAC inhibitors. PROTAC design has been shown to be a useful strategy for selectively degrading protein targets.
Article
Plant Sciences
Madhuvanthi Ramaiah, Ajay Jain, Poli Yugandhar, Kashchandra G. Raghothama
Summary: Ubiquitination-mediated post-translational modification of proteins is a crucial mechanism in plant growth and development. This study found that ATL8 plays a pivotal role in phosphate homeostasis, and its relative expression level is influenced by phosphate and sucrose deficiency. The mutant atl8 showed significant reductions in root traits, root to shoot ratio, and total phosphate content compared to the wild-type under different phosphate regimes. In contrast, the overexpression lines Oe1 and Oe2 exhibited enhancements in some of these traits. Furthermore, ABA and ATL8 were shown to have a cross-talk on root growth and modulate the expression levels of genes involved in phosphate homeostasis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Virology
Lei Hou, Xiaohan Hu, Jinshuo Guo, Rong Quan, Li Wei, Jing Wang, Jiangwei Song, Jue Liu
Summary: In this study, we found that aMPV/C infection induces MAVS degradation through the ubiquitin-proteasome pathway at the post-translational level. Furthermore, specific amino acid sites were identified as pivotal in the formation of polyubiquitin chains during MAVS degradation. E3 ubiquitin ligases targeting MAVS were also identified, with RNF5 shown to be involved in MAVS degradation in aMPV/C-infected cells.
Article
Agriculture, Multidisciplinary
Guangqiang Zhang, Junjiao Yang, Meng Zhang, Qinxue Li, Yunzhen Wu, Xiaoyu Zhao, Huifei Zhang, Yong Wang, Jiajie Wu, Wei Wang
Summary: The study revealed that TaPUB1 enhances the resistance of wheat to Cd stress by modulating the stability of TaIRT1 and TaIAA17 proteins in three ways. TaPUB1 overexpression lines showed reduced Cd accumulation and ROS accumulation, with inhibitory effects on Cd uptake.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Oncology
Rati Lama, Chao Xu, Samuel L. Galster, Javier Querol-Garcia, Scott Portwood, Cory K. Mavis, Federico M. Ruiz, Diana Martin, Jin Wu, Marianna C. Giorgi, Jill Bargonetti, Eunice S. Wang, Francisco J. Hernandez-Ilizaliturri, Gerald B. Koudelka, Sherry R. Chemler, Ines G. Munoz, Xinjiang Wang
Summary: This study identifies MMRi62 as a novel MDM2-MDM4-targeting agent that can induce apoptosis in leukemia cells, including those with non-functional p53.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biology
Sara Scinicariello, Adrian Soderholm, Markus Schaefer, Alexandra Shulkina, Irene Schwartz, Kathrin Hacker, Rebeca Gogova, Robert Kalis, Kimon Froussios, Valentina Budroni, Annika Bestehorn, Tim Clausen, Pavel Kovarik, Johannes Zuber, Gijs A. Versteeg
Summary: Tristetraprolin (TTP) stability is controlled by factors including lysine-ubiquitination and phosphorylation. The E3 ligase HUWE1 was identified as a regulator of TTP stability, indirectly controlling its phosphorylation. This study provides new insights into the regulation of TTP and its impact on immune response.
Article
Oncology
Liangzi Cao, Hao Li, Xiaofang Liu, Yubang Wang, Bowen Zheng, Chengzhong Xing, Naijin Zhang, Jingwei Liu
Summary: This study investigated the molecular alterations and clinical relevance of NEDD4 E3 ligase family genes in 33 cancer types. The findings revealed increased expression of NEDD4 members in pancreatic cancer and decreased expression in thyroid cancer. NEDD4 family genes exhibited a range of mutation frequencies, with HECW1 and HECW2 showing relatively high mutation rates. Breast cancer presented with a high frequency of NEDD4 copy number amplification. Additionally, NEDD4 family members were involved in various pathways such as p53, Akt, apoptosis, and autophagy, and their expression correlated with the survival of cancer patients.
Article
Medicine, General & Internal
Zhonghui Zhang, Liang Zhong, Wenran Dan, Xuan Chu, Chen Liu, Xu Luo, Peng Wan, Zhenyan Liu, Yang Lu, Xiao Wang, Beizhong Liu
Summary: The research reveals that ZFP91 enhances AML cell proliferation and inhibits apoptosis by interacting with RIP1 and inhibiting its K48-linked ubiquitination. These findings provide evidence that targeted inhibition of ZFP91 could be a potential approach to treat AML.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2022)
Article
Plant Sciences
Laurens Pauwels, Andres Ritter, Jonas Goossens, Astrid Nagels Durand, Hongxia Liu, Yangnan Gu, Jan Geerinck, Marta Boter, Robin Vanden Bossche, Rebecca De Clercq, Jelle Van Leene, Kris Gevaert, Geert De Jaeger, Roberto Solano, Sophia Stone, Roger W. Innes, Judy Callis, Alain Goossens
Article
Plant Sciences
Sridhar Ravichandran, Sophia L. Stone, Bernhard Benkel, Junzeng Zhang, Fabrice Berrue, Balakrishnan Prithiviraj
FRONTIERS IN PLANT SCIENCE
(2015)
Article
Biochemistry & Molecular Biology
Daryl McNeilly, Andrew Schofield, Sophia L. Stone
PLANT MOLECULAR BIOLOGY
(2018)
Article
Plant Sciences
Wendy J. Lyzenga, Victoria Sullivan, Hongxia Liu, Sophia L. Stone
FRONTIERS IN PLANT SCIENCE
(2017)
Review
Plant Sciences
Sophia L. Stone
FRONTIERS IN PLANT SCIENCE
(2014)
Article
Plant Sciences
Wendy J. Lyzenga, Myrtle Harrington, Diana Bekkaoui, Merek Wigness, Dwayne D. Hegedus, Kevin L. Rozwadowski
Article
Plant Sciences
Mohammad I. Abo Gamar, Anna Kisiala, R. J. Neil Emery, Edward C. Yeung, Sophia L. Stone, Mirwais M. Qaderi
Article
Plant Sciences
Qiaomu Li, Renata J. Serio, Andrew Schofield, Hongxia Liu, Sheena R. Rasmussen, Daniel Hofius, Sophia L. Stone
Article
Biochemistry & Molecular Biology
Pramod Rathor, Tudor Borza, Sophia Stone, Thierry Tonon, Svetlana Yurgel, Philippe Potin, Balakrishnan Prithiviraj
Summary: This study successfully expressed an unknown function gene, highly responsive to abiotic stresses in Ectocarpus sp., into Arabidopsis thaliana, leading to enhanced tolerance to salt and temperature stresses. The expression of key genes related to abiotic stress tolerance was significantly up-regulated in the transgenic line.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Wendy J. Lyzenga, Curtis J. Pozniak, Sateesh Kagale
Summary: Human population growth has increased the demand for food crops and biomaterials, while climate change is impacting environmental conditions. Therefore, there is a need to develop crop varieties that can tolerate adverse growth conditions while requiring fewer inputs. CRISPR/Cas technology has great potential in plant breeding.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Editorial Material
Biochemical Research Methods
Wendy J. Lyzenga, Sateesh Kagale
Review
Plant Sciences
Wendy J. Lyzenga, Zhigang Liu, Toluwase Olukayode, Yang Zhao, Leon V. Kochian, Byung-Kook Ham
Summary: Enhancing nutrient use efficiency in agriculture requires understanding the molecular features of nutrient uptake by roots, root system architecture, and crop nutrient use efficiency. The main source of essential mineral nutrients for plants is the soil, which plays a crucial role in plant biomolecule synthesis, cell processes, and enzymatic reactions. The roots serve as the main entry point for mineral nutrients, and various transport systems, sensors, and signaling proteins facilitate their uptake. Chemical fertilizers containing nitrogen, phosphorus, and potassium are commonly used to maximize crop yields. However, optimizing nutrient uptake and utilization is essential for sustainable agriculture. Therefore, this review focuses on the molecular basis of N, P, and K nutrient uptake, the relationship between root system architecture and nutrient distribution, and the coordination of nutrient uptake with photosynthesis through the xylem and phloem. The size, structure, and activity of the root system, along with nutrient transporters, determine the rate of nutrient acquisition. The connections between N, P, and K uptake and signaling are also discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Pramod Rathor, Tudor Borza, Yanhui Liu, Yuan Qin, Sophia Stone, Junzeng Zhang, Joseph P. M. Hui, Fabrice Berrue, Agnes Groisillier, Thierry Tonon, Svetlana Yurgel, Philippe Potin, Balakrishnan Prithiviraj
Article
Biochemistry & Molecular Biology
Hongxia Liu, Sophia L. Stone
PLANT SIGNALING & BEHAVIOR
(2014)