Article
Chemistry, Multidisciplinary
Mateusz Kozarski, Karolina Drazkowska, Marcelina Bednarczyk, Marcin Warminski, Jacek Jemielity, Joanna Kowalska
Summary: mRNA-based gene delivery is a powerful strategy for vaccines and therapeutics. This study synthesized novel triazole-containing tri- and tetranucleotide cap analogs through copper-catalyzed azide-alkyne cycloaddition. One of the compounds showed translational activity and biochemical properties comparable to natural cap structure, making it a promising mRNA capping reagent.
Article
Microbiology
Yanwei Gong, Ying Li, Dongyang Liu, Lianqiang Jiang, Hui Liang, Yuanhua Wu, Fenglong Wang, Jinguang Yang
Summary: Kac is a model for all acylation modification studies and is involved in six major biological functions. In this study, we investigated the acetylation modification of proteins in healthy and TSWV-infected N. benthamiana leaves. Our results showed that acetylation of proteins was significantly altered after the infection, with upregulated and downregulated sites. We also identified conserved motifs and found that most of the Kac proteins were located in the chloroplast and cytoplasm, participating in cellular and metabolic processes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Kristin Widyasari, John Bwalya, Kook-Hyung Kim
Summary: Infection of Nicotiana benthamiana by viruses from the genera Bromovirus, Potyvirus, and Potexvirus induces up-regulation of genes encoding the HSP70 family, including BiP2. Knockdown of three up-regulated genes showed that Bip2 is important for potyvirus infection but not for the other tested viruses. Knockdown of BiP2 resulted in reduced accumulation of SMV and PepMoV RNA, and it was found to interact with SMV's NIb protein. Therefore, BiP2 plays a crucial role as a proviral host factor in potyvirus infection.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Sayaka Imano, Mayuka Fushimi, Maurizio Camagna, Akiko Tsuyama-Koike, Hitoshi Mori, Akira Ashida, Aiko Tanaka, Ikuo Sato, Sotaro Chiba, Kazuhito Kawakita, Makoto Ojika, Daigo Takemoto
Summary: In this study, the expression profiles of N. benthamiana genes after INF1 treatment were analyzed using RNAseq analysis. The researchers identified key genes and regulatory factors induced by INF1, providing important data for the study of plant resistance mechanisms against pathogens.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Immunology
Jutamas Shaughnessy, Aleyo Chabeda, Y. Tran, Bo Zheng, Nancy Nowak, Carolynn Steffens, Rosane B. DeOliveira, Sunita Gulati, Lisa A. Lewis, James Maclean, John A. Moss, Keith L. Wycoff, Sanjay Ram
Summary: This study investigated novel therapeutics against multidrug-resistant Neisseria gonorrhoeae. By modifying the binding between antibodies and factor H (FH), the researchers successfully enhanced bactericidal activity. The chimeric protein Fc3/FH* showed high killing efficacy against diverse strains and demonstrated promising prophylactic potential in a mouse model.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Plant Sciences
Areum Yun, Joohyun Kang, Juhun Lee, Shi-Jian Song, Inhwan Hwang
Summary: Plants are advantageous for producing recombinant proteins due to their low cost and scalability. However, current plant protein expression systems need improvement in achieving high yields. In this study, a robust expression system was developed using a hybrid promoter, FM ' M-UD, and an artificial terminator, 3PRt, which resulted in significant increases in protein levels compared to traditional promoters. Furthermore, the combination of FM ' M-UD/3PRt with an artificial transcription factor further enhanced protein production. Overall, this novel expression system allows for the efficient production of large quantities of recombinant proteins in plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xuwei Chen, Xinyang Wu, Shiyou Qiu, Hongying Zheng, Yuwen Lu, Jiejun Peng, Guanwei Wu, Jianping Chen, Shaofei Rao, Fei Yan
Summary: In this study, 14 members of the BZR family were identified in the Nicotiana benthamiana genome, showing diverse expression patterns in different tissues and playing multiple roles in response to abiotic stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Christos Karampelias, Kathleen Watt, Charlotte L. Mattsson, Angel Fernandez Ruiz, Habib Rezanejad, Jiarui Mi, Xiaojing Liu, Lianhe Chu, Jason W. Locasale, Gregory S. Korbutt, Meritxell Rovira, Ola Larsson, Olov Andersson
Summary: This study identifies CID661578 as a small molecule that can induce pancreatic beta-cell regeneration by targeting MNK2 and enhancing protein synthesis. This finding reveals a novel targetable role of MNK2-controlled translation in beta-cell regeneration.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Neurosciences
Shane Wiebe, Ziying Huang, Reese Jalal Ladak, Agnieszka Skalecka, Roberta Cagnetta, Jean-Claude Lacaille, Argel Aguilar-Valles, Nahum Sonenberg
Summary: The consolidation of learned information into long-lasting memories requires the strengthening of synaptic connections through protein synthesis. Translation initiation factors are crucial for regulating memory formation. The role of the translation inhibitor 4EHP in learning and memory was unknown. Conditional knockout mice for 4EHP were generated and behavioral memory tasks were analyzed. The results showed that 4EHP is necessary for working memory, mediated by mTORC1 activity in excitatory and inhibitory neurons.
Article
Plant Sciences
Xuan Xu, Gea Guerriero, Roberto Berni, Kjell Sergeant, Cedric Guignard, Audrey Lenouvel, Jean-Francois Hausman, Sylvain Legay
Summary: The present study aims to assess the role and function of MdMYB52 in the suberization process of apple skin. Through transcriptomic analysis, analytical chemistry, and microscopy, it was found that MdMYB52 is involved in the synthesis of suberin phenolic compounds and cell senescence. These findings contribute to a better understanding of the regulatory mechanisms in the suberization process of apple skin.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Xuan Xu, Gea Guerriero, Frederic Domergue, Olga Beine-Golovchuk, Emmanuelle Cocco, Roberto Berni, Kjell Sergeant, Jean-Francois Hausman, Sylvain Legay
Summary: Apple russeting is caused by the accumulation of suberin in the cell wall due to defects and damages in the cuticle layer. MdMYB68, a transcription factor from the MYB family, has been identified as a regulator of suberin biosynthesis in apple fruit skin. Overexpression of MdMYB68 triggers the expression of the suberin biosynthesis pathway, regulates lipid deposition in cell walls, and causes significant modifications in the hemicelluloses and pectins.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biology
F. E. N. G. ZHU, Q. I. N. Q. I. N. ZHANG, Y. A. N. G. K. A. ZHOU, Q. I. P. I. N. G. ZHANG, M. E. N. G. Y. A. O. CAO, Z. H. A. O. L. I. N. JI
Summary: The NAC superfamily is a large plant-specific family of transcription factors, and recent studies have shown that NAC1 plays an important role in plant response to biotic and abiotic stimuli. In this study, researchers focused on developing a prokaryotic expression system to produce the NbNAC1 protein in Escherichia coli and preparing its polyclonal antibody. They successfully expressed and purified the NbNAC1 protein using two different expression vectors, and chose the His-tagged NbNAC1 protein for immunization to generate a highly specific anti-NbNAC1 polyclonal antibody.
Article
Plant Sciences
Wanuttha Boonyayothin, Sirorut Sinnung, Balamurugan Shanmugaraj, Yoshito Abe, Richard Strasser, Prasit Pavasant, Waranyoo Phoolcharoen
Summary: The study demonstrated the potential of the plant-produced denosumab, expressed transiently in Nicotiana benthamiana using the geminiviral expression system, to inhibit osteoclast differentiation and significantly reduce the number of osteoclasts.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Hua Jiang, Shengyu Gu, Kai Li, Junyi Gai
Summary: The TGA transcription factors in soybeans have shown potential for supporting basal resistance to Soybean mosaic virus, with genes such as GmTGA8 and GmTGA19 standing out for their effectiveness in inhibiting virus multiplication. Future research will explore the mechanisms behind their resistance and their potential for improving virus resistance in soybeans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Mireia Uranga, Jose-Antonio Daros
Summary: Using plant RNA viruses as transient delivery vectors is an improved method for CRISPR-Cas genome editing, allowing for rapid editing in a short period of time. This method shows potential for enhancing plant resistance using CRISPR-Cas technology. However, there are still challenges and limitations to be addressed.
Article
Agronomy
Fernando Martinez-Moreno, Patricia Giraldo, Cristina Nieto, Magdalena Ruiz
Summary: A study evaluated the resistance of 84 bread wheat Spanish landraces to leaf rust and yellow rust. It was found that resistant landraces mostly originated from the north-west region with higher precipitation and more uniform temperatures.
Article
Biochemistry & Molecular Biology
German Murcia, Cristina Nieto, Romina Sellaro, Salome Prat, Jorge J. Casal
Summary: Hypocotyl growth in Arabidopsis thaliana during the night is not only influenced by the current temperature, but also by preceding daytime temperatures, indicating a short-term memory of previous conditions. Transcriptional regulator ELF3 and factors PIF4 and HY5 play crucial roles in this temperature-dependent growth response.
Article
Plant Sciences
Cristina Alcaide, Livia Donaire, Miguel A. Aranda
Summary: In this study, an antagonistic interaction between different strains of Pepino mosaic virus (PepMV) in tomato plants was described. Transcriptomic analyses revealed that mixed infections caused greater transcriptomic alterations compared to single infections, especially at early infection times. The study also found that PepMV-EU differentially deregulates host gene expression, leading to repression of both strains.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Maryam Khalili, Thierry Candresse, Igor Koloniuk, Dana Safarova, Yoann Brans, Chantal Faure, Marine Delmas, Sebastien Massart, Miguel A. Aranda, Kadriye Caglayan, Veronique Decroocq, Pavlina Drogoudi, Miroslav Glasa, George Pantelidis, Milan Navratil, Francois Latour, Josef Spak, Jaroslava Pribylova, Daniel Mihalik, Francesco Palmisano, Antonella Saponari, Tomas Necas, Jiri Sedlak, Armelle Marais
Summary: Through high-throughput sequencing, four new luteovirus species infecting peach trees were identified. A survey in eight European countries showed that peach-associated luteovirus (PaLV), nectarine stem pitting-associated virus (NSPaV), and a novel luteovirus, peach-associated luteovirus 2 (PaLV2), are prevalent, with NSPaV being the most common virus.
Article
Biotechnology & Applied Microbiology
Giuliano S. Pechar, Livia Donaire, Blanca Gosalvez, Carlos Garcia-Almodovar, Maria Amelia Sanchez-Pina, Veronica Truniger, Miguel A. Aranda
Summary: The study used CRISPR/Cas9 genome editing to knockout the eif4e gene in melon and found that homozygous mutant plants showed resistance to virus and displayed a male sterility phenotype. RNA-Seq analysis revealed the critical role of eIF4E in the formation of male gametes in melon.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Manuel Miras, Miguel A. Aranda, Veronica Truniger
Summary: Most plant viruses lack the 5'-cap and 3'-poly(A) structures found in their host mRNAs and have developed alternative translation initiation mechanisms. In this study, the translation initiation mechanism of cucurbit aphid-borne yellows virus (CABYV), a member of poleroviruses, was investigated. The presence of a 3'-CITE in the 3'-UTR of CABYV was found to be necessary for efficient translation. Additionally, it was demonstrated that the 3'-CITE's activity is dependent on the presence of the 5'-UTR in cis. The 3'-CITEs of different CABYV isolates have evolved through recombination, potentially adapting to specific geographical regions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Ayoub Maachi, Yolanda Hernando, Miguel A. Aranda, Livia Donaire
Summary: We identified and characterized a new virus, named cohombrillo-associated virus (CoAV), that infects Ecballium elaterium plants. The genome of CoAV consists of two molecules of positive-sense RNA, with lengths of 6,934 and 3,501 nucleotides. Phylogenetic analysis suggests that CoAV belongs to a new species in the Stralarivirus genus and is closely related to strawberry latent ringspot virus.
ARCHIVES OF VIROLOGY
(2023)
Article
Plant Sciences
Eduardo Mendez-Lopez, Livia Donaire, Blanca Gosalvez, Pedro Diaz-Vivancos, M. Amelia Sanchez-Pina, Jens Tilsner, Miguel A. Aranda
Summary: This study identified an interaction between the tomato glutathione S-transferase (GST) SlGSTU38 and the PepMV coat protein (CP) using yeast two-hybrid screening. It was found that SlGSTU38 functions as a susceptibility factor for PepMV and relocalizes to virus replication complexes during infection. Knocking out SlGSTU38 also leads to reactive oxygen species accumulation and the deregulation of stress-responsive genes.
Article
Plant Sciences
Mei Liu, Baoshan Kang, Huijie Wu, Miguel A. Aranda, Bin Peng, Liming Liu, Zhangjun Fei, Ni Hong, Qinsheng Gu
Summary: This study reveals that SA-induced flavonoid biosynthesis plays a critical role in watermelon development and resistance to CGMMV. Understanding the defense mechanism of watermelon against CGMMV infection is essential for managing viral diseases in agriculture.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Virology
Cristina Alcaide, Miguel A. Aranda
Summary: Mixed infections of Pepino mosaic virus (PepMV) isolates from the EU and CH2 strains are common in tomato crops. In this study, fluorescent protein-labeled PepMV clones were used to investigate the interactions between these strains, and flow cytometry was used to determine the proportion of cells infected with each isolate in single and mixed infected plants.
Article
Microbiology
Nuria Fontdevila Pareta, Maryam Khalili, Ayoub Maachi, Mark Paul S. Rivarez, Johan Rollin, Ferran Salavert, Coline Temple, Miguel A. Aranda, Neil Boonham, Marleen Botermans, Thierry Candresse, Adrian Fox, Yolanda Hernando, Denis Kutnjak, Armelle Marais, Francoise Petter, Maja Ravnikar, Ilhem Selmi, Rachid Tahzima, Charlotte Trontin, Thierry Wetzel, Sebastien Massart
Summary: Advancements in HTS technologies and bioinformatic tools have led to the discovery and publication of new viral sequences at an unprecedented rate. In response, a collective effort was made to propose a framework for prioritizing the characterization of newly discovered plant viruses. An updated framework was developed to incorporate emerging trends and tools, providing a more efficient and comprehensive approach to address knowledge and data gaps in virus discovery.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Chenchen Liu, Xiliang Jiang, Zhaoyan Tan, Rongqun Wang, Qiaoxia Shang, Hongrui Li, Shujin Xu, Miguel A. Aranda, Beilei Wu
Summary: In fungi, viral infections may not cause phenotypic changes and their role in the emergence of opportunistic species is not well-known. This study identified a novel mycovirus in Trichoderma isolated from grassland soils in Inner Mongolia, China.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Fabiola Ruiz-Ramon, Pascual Rodriguez-Sepulveda, Pau Breto, Livia Donaire, Yolanda Hernando, Miguel A. Aranda
Summary: The OSCA4.1 channel protein plays a crucial role in tomato resistance to pepino mosaic virus (PepMV). A mutant with resistance to PepMV was identified through screening, and the resistance locus was mapped to chromosome 2. Further study revealed that OSCA4.1 may be involved in the early stages of viral infection.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Review
Agriculture, Multidisciplinary
Yolanda Hernando, Miguel A. Aranda
Summary: Cross-protection is a method of protecting plants from aggressive viral isolates by pre-inoculating them with mild or attenuated viral isolates. While it has been shown to be effective against various virus species infecting different plants, its use in crop damage control is limited. PepMV cross-protection has been successful, but its viability may be threatened by the interaction with the tomato brown rugose fruit virus. This review describes the experience of deploying cross-protection against PepMV in Spain and emphasizes the careful consideration and preparation needed for its successful implementation.
ANNALS OF APPLIED BIOLOGY
(2023)
Article
Virology
Cristina Alcaide, Eduardo Mendez-Lopez, Jesus R. Ubeda, Pedro Gomez, Miguel A. Aranda
Summary: This study aimed to characterize two aggressive PepMV isolates and identify amino acid substitutions associated with severe symptoms. By introducing a specific amino acid substitution, we successfully created a mild PepMV isolate that causes symptoms similar to the aggressive isolates.
