Article
Biochemistry & Molecular Biology
Marina Martinez-Garcia, Charles I. White, F. Chris. H. Franklin, Eugenio Sanchez-Moran
Summary: TOPII plays an essential endogenous role in normal root growth, cell cycle regulation, mitotic DNA repair through homologous recombination, and meiotic DNA repair progression in Arabidopsis thaliana. While it is required for meiotic double-strand break repair, it does not affect crossover formation. It is proposed that TOPII may facilitate mitotic homologous recombination DNA repair by relieving stress necessary for strand invasion and D-loop formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Mi Ae Kang, Jong-Soo Lee
Summary: CTCF, a highly conserved multifunctional DNA-binding protein with 11 zinc fingers, plays crucial roles in diverse genomic processes such as transcriptional regulation, insulation, genome imprinting, and maintenance of genome organization. Recent findings reveal that CTCF is involved in DNA double-strand break (DSB) repair through homologous recombination (HR), facilitating accurate restoration of broken DNA sequences. Understanding the functional crosstalks between CTCF and other HR factors may shed light on the molecular basis of various human diseases and the diverse functions of CTCF in genome biology.
Article
Plant Sciences
Xiang Li, Mingsen Yu, Pablo Bolanos-Villegas, Jun Zhang, Di'an Ni, Hong Ma, Yingxiang Wang
Summary: The study revealed the crucial role of FANCM in shaping the distribution of meiotic Class-I crossovers in plants, with evolutionary divergent roles observed between Arabidopsis and lettuce in meiotic bivalent formation.
Article
Multidisciplinary Sciences
Yu Jiang, Fei Huang, Lu Chen, Jia-Hui Gu, Yun-Wen Wu, Meng-Yan Jia, Zhen Lin, Yong Zhou, Yan-Chu Li, Chao Yu, Ming-Han Tong, Li Shen, Heng-Yu Fan, Qian-Qian Sha
Summary: The R-loop, a three-strand nucleic acid structure, plays important regulatory roles in the meiosis process, particularly in transcriptional regulation and DNA repair. The study of R-loop in mice revealed its predominant distribution at promoter-related regions, its association with high transcription activity, and its crucial role in fertility and DSB repair. These findings highlight the significance of R-loop in spermatogenesis.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Multidisciplinary Sciences
Donna R. Whelan, Eli Rothenberg
Summary: Using single-molecule localization super-resolution imaging assays, we observed the spatiotemporal behavior of key mediator and nuclease proteins during DNA resection at single-ended double-strand breaks. Multiple simultaneous resection events were demonstrated, with recruitment of various proteins and completion of resection 2 to 4 hours after break induction. Additionally, we identified potential roles of BRCA1 and BLM in homology search and repair resolution during HR.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Jovanka Vladejic, Fen Yang, Eva Dvorak Tomastikova, Jaroslav Dolezel, Jan J. Palecek, Ales Pecinka
Summary: Plants have a sophisticated molecular network to maintain the integrity of genetic information and repair damaged DNA. This study identified and analyzed four uncharacterized Arabidopsis BCP proteins, with BCP1 being the most interesting candidate due to its sensitivity to DNA damage. It was found that BCP1 is regulated by SOG1 and is involved in homology-based repair.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Xuanpeng Wang, Lili Wang, Yongchi Huang, Zhiping Deng, Cunliang Li, Jian Zhang, Mingxi Zheng, Shunping Yan
Summary: This study reveals the important role of a plant-specific ubiquitin E3 ligase, DDRM1, in homologous recombination repair (HR), which is essential for maintaining genome stability. DDRM1 interacts with and ubiquitinates the transcription factor SOG1, promoting its stability. This research uncovers the regulatory mechanism of HR in plants and highlights the significance of ubiquitination in HR.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Julia Dluzewska, Wojciech Dziegielewski, Maja Szymanska-Lejman, Monika Gazecka, Ian R. Henderson, James D. Higgins, Piotr A. Ziolkowski
Summary: The study reveals that MSH2 has antagonistic effects on interfering and non-interfering crossover pathways in Arabidopsis, acting as a master regulator of meiotic DSB repair. This finding highlights the importance of MSH2 in shaping the crossover landscape in relation to interhomolog polymorphism.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Jiaojiao Deng, Sophia B. Chernikova, Yuelong Wang, Mirna L. Rodriguez, Stephanie J. Andersen, Maxine C. Umeh-Garcia, Bryanna O. Godfrey, Saman S. Ahmadian, Wolf-Nicolas Fischer, Kerry J. Koller, Bernd Jandeleit, Gordon M. Ringold, Melanie Hayden Gephart
Summary: Metastasis to the central nervous system from breast cancer, especially triple-negative breast cancer, presents a significant clinical challenge due to limited treatment options and rapidly fatal outcomes. Novel therapeutic options that can effectively target brain metastasis and leptomeningeal spread are urgently needed in order to improve patient outcomes.
MOLECULAR CANCER THERAPEUTICS
(2021)
Review
Oncology
Takayuki Saitoh, Tsukasa Oda
Summary: Multiple myeloma is an incurable plasma cell malignancy characterized by genomic instability. The tumor microenvironment and abnormal DNA repair function play a role in genetic instability in this disease. Factors in the tumor microenvironment, such as inflammation and hypoxia, significantly influence DNA repair pathways in multiple myeloma.
Article
Biochemistry & Molecular Biology
Andrea Holickova, Jan Roska, Eveline Orasova, Vladimira Bruderova, Patrik Palacka, Dana Jurkovicova, Miroslav Chovanec
Summary: Cisplatin-based chemotherapy is commonly used for muscle-invasive bladder cancer, but many cases develop resistance or do not respond. This study found that the response to cisplatin in urothelial carcinoma cells is dependent on DNA damage repair and tolerance factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiaofei Liu, Yiwei Cao, Guijie Du, Chao Zhang, Meng Xu, Zhukuan Cheng, Yi Shen, Hengxiu Yu
Summary: The mechanisms of OsRAD51 and OsDMC1 in rice meiosis are still unknown. In this study, mutant plants for Osrad51a1, Osrad51a2, Osdmc1a, and Osdmc1b were obtained through CRISPR/Cas9, and it was found that OsRAD51A1 and OsRAD51A2 are functionally redundant in rice fertility. The study also suggests that OsRAD51 and OsDMC1 may functionally depend on each other and play important roles in meiotic recombination during meiosis in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Genetics & Heredity
Lea Lingg, Sven Rottenberg, Paola Francica
Summary: Tumor cells often have genetic alterations that affect tumor progression and DNA repair. Reactivation of germ cell-specific genes in tumors influences DNA repair and response to treatment. Targeting aberrantly expressed meiotic genes may provide a unique opportunity to kill cancer cells specifically.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Benyu Su, David Lim, Chenyang Qi, Zhongwei Zhang, Junxiao Wang, Fengmei Zhang, Chao Dong, Zhihui Feng
Summary: Cell cycle checkpoint kinases play a crucial role in protecting against replicative stress. Valproic acid (VPA), a histone deacetylase inhibitor, was found to promote breast cancer cell progression into G2/M phase while protecting normal cells by regulating PPP2R2A and Chk1 phosphorylation. The expression levels of PPP2R2A and pChk1 were also associated with patient survival.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Hellida Marina Costa-Silva, Bruno Carvalho Resende, Adriana Castilhos Souza Umaki, Willian Prado, Marcelo Santos da Silva, Stela Virgilio, Andrea Mara Macedo, Sergio Danilo Junho Pena, Erich Birelli Tahara, Luiz Ricardo Orsini Tosi, Maria Carolina Elias, Luciana Oliveira Andrade, Joao Luis Reis-Cunha, Gloria Regina Franco, Stenio Perdigao Fragoso, Carlos Renato Machado
Summary: DNA topoisomerases, in particular topoisomerase 3 alpha, play crucial roles in genomic maintenance, cell growth, and DNA damage response in Trypanosoma cruzi. Knockout of topoisomerase 3 alpha affects parasite growth, invasion rates, and ability to repair DNA damage induced by genotoxic agents. These findings underscore the importance of topoisomerase 3 alpha in homologous recombination repair and replication stress in T. cruzi.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Evelyn Rampler, Thomas Stranzl, Zsuzsanna Orban-Nemeth, David Maria Hollenstein, Otto Hudecz, Peter Schloegehofer, Karl Mechtler
JOURNAL OF PROTEOME RESEARCH
(2015)
Article
Biochemical Research Methods
Elisabeth Roitinger, Manuel Hofer, Thomas Koecher, Peter Pichler, Maria Novatchkova, Jianhua Yang, Peter Schloegelhofer, Karl Mechtler
MOLECULAR & CELLULAR PROTEOMICS
(2015)
Correction
Biochemical Research Methods
Evelyn Rampler, Thomas Stranzl, Zsuzsanna Orban-Nemeth, David Maria Hollenstein, Otto Hudecz, Peter Schlogelhofer, Karl Mechtler
JOURNAL OF PROTEOME RESEARCH
(2016)
Letter
Biochemical Research Methods
Zsuzsanna Orban-Nemeth, Rebecca Beveridge, David M. Hollenstein, Evelyn Rampler, Thomas Stranzl, Otto Hudecz, Johannes Doblmann, Peter Schloegelhofer, Karl Mechtler
Article
Biochemical Research Methods
Zsuzsanna Orban-Nemeth, Rebecca Beveridge, David M. Hollenstein, Evelyn Rampler, Thomas Stranzl, Otto Hudecz, Johannes Doblmann, Peter Schloegelhofer, Karl Mechtler
Article
Biochemistry & Molecular Biology
Marie-Therese Kurzbauer, Mnica Pradillo, Claudia Kerzendorfer, Jason Sims, Rene Ladurner, Cecilia Oliver, Michael Peter Janisiw, Magdalena Mosiolek, Dieter Schweizer, Gregory P. Copenhaver, Peter Schloegelhofer
Article
Biochemistry & Molecular Biology
Clemens Uanschou, Arnaud Ronceret, Mona Von Harder, Arnaud De Muyt, Daniel Vezon, Lucie Pereira, Liudmila Chelysheva, Wataru Kobayashi, Hitoshi Kurumizaka, Peter Schloegelhofer, Mathilde Grelon
Article
Multidisciplinary Sciences
Gabriela Cabral, Andre Marques, Veit Schubert, Andrea Pedrosa-Harand, Peter Schloegelhofer
NATURE COMMUNICATIONS
(2014)
Article
Biochemistry & Molecular Biology
Wataru Kobayashi, Enwei Liu, Hajime Ishii, Sachihiro Matsunaga, Peter Schloegelhofer, Hitoshi Kurumizaka
JOURNAL OF BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Jason Sims, Gregory P. Copenhaver, Peter Schloegelhofer
Article
Biochemistry & Molecular Biology
Chao Yang, Kostika Sofroni, Erik Wijnker, Yuki Hamamural, Lena Carstens, Hirofumi Harashima, Sara Christina Stolze, Daniel Vezon, Liudmila Chelysheva, Zsuzsanna Orban-Nemeth, Gaetan Pochon, Hirofumi Nakagami, Peter Schloegelhofer, Mathilde Grelon, Arp Schnittger
Article
Multidisciplinary Sciences
Jason Sims, Giovanni Sestini, Christiane Elgert, Arndt von Haeseler, Peter Schloegelhofer
Summary: Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). Using a combination of long- and short-read sequencing, researchers have identified tissue-specific variants of rRNA genes integrated into translating ribosomes in Arabidopsis NOR2 rDNA.
NATURE COMMUNICATIONS
(2021)
Review
Plant Sciences
Jason Sims, Fernando A. Rabanal, Christiane Elgert, Arndt Von Haeseler, Peter Schloegelhofer
Summary: Ribosomal RNA genes are organized in large domains of hundreds of rDNA units, with stable inheritance crucial for survival. During meiosis, rDNA loci are embedded within the nucleolus favoring non-homologous end joining as a repair mechanism, while rare homologous recombination events dynamically change rDNA unit numbers. The sequence heterogeneity and size stability of rDNA clusters in Arabidopsis thaliana show potential functional implications and drive further research into the mechanisms maintaining their metastable state.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Marie-Therese Kurzbauer, Michael Peter Janisiw, Luis F. Paulin, Ignacio Prusen Mota, Konstantin Tomanov, Ondrej Krsicka, Arndt von Haeseler, Veit Schubert, Peter Schloegelhofer
Summary: The study shows that Arabidopsis ATM plays a role in multiple steps of meiosis, limiting the number of meiotic DSBs and affecting CO formation and SC organization. This extends our understanding of how ATM functions during plant meiosis.
Article
Biochemistry & Molecular Biology
Zdravko J. Lorkovic, Chulmin Park, Malgorzata Goiser, Danhua Jiang, Marie-Therese Kurzbauer, Peter Schloegelhofer, Frederic Berger