Review
Biochemistry & Molecular Biology
Edwin Jarratt-Barnham, Limin Wang, Youzheng Ning, Julia M. Davies
Summary: Plant CNGCs are tetrameric cation channels activated by cNMPs like cAMP and cGMP. Arabidopsis thaliana encodes 20 CNGC subunits involved in development, stress response, and immunity. Recent findings show that CNGC subunits form heterotetrameric complexes with distinct behavior, affecting signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jian Sun, Youzheng Ning, Limin Wang, Katie A. Wilkins, Julia M. Davies
Summary: Extracellular ATP (eATP) is considered a constitutive damage-associated molecular pattern (DAMP) that is released in response to injury, herbivory, or pathogen attack. Its concentration must be tightly regulated to prevent cell death. In Arabidopsis thaliana, the perception of eATP is mediated by plasma membrane receptors and involves cyclic nucleotides, potentially leading to an increase in cytosolic-free Ca2+ as a second messenger.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yi Ma, Keila Garrido, Rashid Ali, Gerald A. Berkowitz
Summary: Plant CNGCs play a crucial role in cytosolic Ca2+ influx for immune defense and thermosensing. Physically interacting CNGCs (CNGC2, CNGC4, and CNGC6) may form a multimeric channel complex involved in immune signaling, while CNGC12 does not interact with other CNGCs. CNGC2, CNGC4, and CNGC6 mutants showed impaired response to the elicitor peptide Pep3, whereas all four CNGC mutants exhibited thermotolerance.
Article
Plant Sciences
Ha N. Duong, Sung-Hwan Cho, Limin Wang, An Q. Pham, Julia M. Davies, Gary Stacey
Summary: Extracellular ATP is regarded as a danger signal in plants and animals, and its recognition by the PM-localized receptor P2K1 leads to a rapid increase in cytoplasmic calcium levels through the crucial involvement of CNGC6. Mutations in the CNGC6 gene decrease plant responses to eATP, including reduced MAPK activation, gene expression, and increased susceptibility to pathogens.
Article
Biochemistry & Molecular Biology
Dongli Yu, Wen Song, Eddie Yong Jun Tan, Li Liu, Yu Cao, Jan Jirschitzka, Ertong Li, Elke Logemann, Chenrui Xu, Shijia Huang, Aolin Jia, Xiaoyu Chang, Zhifu Han, Bin Wu, Paul Schulze-Lefert, Jijie Chai
Summary: This study reveals that plant Toll/interleukin-1 receptor (TIR) proteins not only have NADase function, but also act as 2',3'-cAMP/cGMP synthetases. These cyclic nucleotide monophosphates (cNMPs) play a critical role in plant immune responses.
Article
Plant Sciences
Soeui Lee, Hye-Young Lee, Hui Jeong Kang, Ye-Eun Seo, Joo Hyun Lee, Doil Choi
Summary: Transient and rapid increase in cytosolic Ca2+ is crucial for plant defense against pathogens. This study reveals that cyclic nucleotide-gated channels (CNGCs) play a key role in mediating Ca2+ influx during this process, and AVRblb2 interacts with CNGCs via calmodulin and calmodulin-like proteins. Moreover, Avrblb2 inhibits calcium channel activation by preventing the release of calmodulin and calmodulin-like proteins. The formation of active heteromeric channels with other CNGCs allows fine-tuning of cytosolic Ca2+ level for appropriate responses to diverse signals.
Article
Plant Sciences
Chunhui Zhao, Yinhua Tang, Junli Wang, Yanhong Zeng, Hequan Sun, Zichao Zheng, Rong Su, Korbinian Schneeberger, Jane E. Parker, Haitao Cui
Summary: The study reveals the critical role of calcium (Ca2+) as a second messenger in plant immunity, with mutations in the CNGC20 gene affecting the cells' disease resistance responses. The research also highlights the mechanism by which CNGC20 influences PTI and ETI responses, emphasizing the importance of tight control of the CNGC20 calcium ion channel in immune regulation.
Article
Plant Sciences
Yajie Zhang, Yubo Li, Jing Yang, Xinli Yang, Shengbei Chen, Zhouli Xie, Mingjie Zhang, Yanlei Huang, Jinghong Zhang, Xing Huang
Summary: This study identified 43 CNGC genes with tissue-specific expression patterns in mango. Five of these genes showed significant upregulation in the fruit peel and leaf under cold stress. Furthermore, a correlation was found between these genes and cell membrane integrity, suggesting their role in regulating cell membrane integrity during cold stress.
Article
Plant Sciences
Limin Wang, Youzheng Ning, Jian Sun, Katie A. Wilkins, Elsa Matthus, Rose E. McNelly, Adeeba Dark, Lourdes Rubio, Wolfgang Moeder, Keiko Yoshioka, Anne-Alienor Very, Gary Stacey, Nathalie Leblanc-Fournier, Valerie Legue, Bruno Moulia, Julia M. Davies
Summary: Extracellular ATP can induce an increase in cytosolic free calcium ion as a second messenger, and CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) is identified as a critical component linking extracellular ATP receptors to calcium ion signaling in roots.
Article
Biotechnology & Applied Microbiology
Nannan Zhang, Huanzhang Lin, Qiaoying Zeng, Danwen Fu, Xiaoning Gao, Jiayun Wu, Xiaomin Feng, Qinnan Wang, Qiuping Ling, Zilin Wu
Summary: In this study, 16 CNGC genes and their alleles were identified and classified from Saccharum spontaneum. Gene duplication and syntenic relationships analysis showed that the CNGC gene family in S. spontaneum expanded mainly through segmental duplication events. Most of the SsCNGCs showed differential expression during development and in different tissues, with a diurnal rhythm. SsCNGC13 gene might be involved in both sugarcane development and its response to low-K+ stress.
Article
Plant Sciences
Emily Tipper, Nuno Leitao, Pierre Dangeville, David M. Lawson, Myriam Charpentier
Summary: This study investigates the role of AtCNGC15 in root meristem development and nitrate-induced gene expression. It is found that nuclear calcium release, mediated by AtCNGC15, can modulate root development and gene expression. In addition, the localization of AtCNGC15 changes during root growth, with nuclear localization in early stages and plasma membrane localization in later stages.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Xiao Fan, Jian Huang, Xueqin Jin, Nieng Yan
Summary: Voltage-gated sodium channel Nav1.6 is crucial for neuronal firing and its dysfunction can lead to epilepsy and neurological disorders. The cryo-EM structure of human Nav1.6 reveals its inactivated state with closed pore domain and all up voltage-sensing domains. The presence of an unidentified molecule bound to the Nav1.6 PD suggests a potential target for developing specific blockers.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Iris Hardege, Julia Morud, Jingfang Yu, Tatiana S. Wilson, Frank C. Schroeder, William R. Schafer
Summary: Betaine, a derivative of amino acid, has been found in diverse organisms and has well-established functions as a methyl donor and osmolyte. It is also present in the nervous system, but its role there is not well understood. A study on nematode worm, Caenorhabditis elegans, shows that betaine is synthesized in its nervous system and plays a role in controlling different behavioral states. It is produced in specific interneurons and is packed into synaptic vesicles by the vesicular monoamine transporter. The study also reveals a betaine-gated chloride channel that is broadly expressed in the navigation circuit. These findings suggest that neuronally produced betaine acts as a neuromodulator and may have a similar role in other animal nervous systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Shuo Han, Xiang-Ping Chu, Ryan Goodson, Prae Gamel, Sophia Peng, Joshua Vance, Shizhen Wang
Summary: Cholesterol inhibits the hHv1 channel by stabilizing the voltage-sensing S4 segment at resting conformations, providing a mechanism for the regulation of fertilization by the hHv1 channel.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Physical
Zhongwu Li, Quan Han
Summary: This study presents molecular dynamics simulations of gated ion transport in two disjoint carbon nanotubes (CNTs) with a nanogap. It is observed that the nanogap acts as a gate, allowing ions to transport only above a certain voltage. Interestingly, a water bridge forms within the nanogap above a specific voltage, facilitating ion transport in disjoint CNTs. Furthermore, the gated ion transport behavior is influenced by the nanogap length, CNT diameter, and CNT wettability, but not by the ion concentration.
Article
Biochemistry & Molecular Biology
Xiao Yu, Guangyuan Xu, Bo Li, Luciano de Souza Vespoli, Hai Liu, Wolfgang Moeder, Sixue Chen, Marcos V. V. de Oliveira, Suzane Ariadina de Souza, Wenyong Shao, Barbara Rodrigues, Yi Ma, Shweta Chhajed, Shaowu Xue, Gerald A. Berkowitz, Keiko Yoshioka, Ping He, Libo Shan
Letter
Plant Sciences
Petra Dietrich, Wolfgang Moeder, Keiko Yoshioka
Article
Plant Sciences
Kai Wang, Ruth W. Ndathe, Narender Kumar, Elizabeth A. Zeringue, Naohiro Kato, John C. Larkin
Editorial Material
Multidisciplinary Sciences
Keiko Yoshioka, Wolfgang Moeder
Article
Plant Sciences
Purva Karia, Keiko Yoshioka, Wolfgang Moeder
Summary: TTM1 is specifically involved in senescence induced by ABA, and its function and turnover are regulated by multiple MAP kinases, with phosphorylation of Ser437 being essential for its function in senescence. Multiple MAP kinases mark TTM1 for protein turnover by phosphorylating Ser10 and Ser490 as well, preventing uncontrolled cell death. This study reveals a novel link between mitochondria and PCD during senescence-associated cell death.
Article
Biotechnology & Applied Microbiology
Tsuyoshi Tanaka, Yoshiaki Maeda, Noraiza Suhaimi, Chiharu Tsuneoka, Tomomi Nonoyama, Tomoko Yoshino, Naohiro Kato, Kyle J. Lauersen
Summary: The study found the phenomenon of intron mediated enhancement (IME) in the oleaginous diatom, Fistulifera solaris, showing that inclusion of introns in heterologous sequences can significantly increase transgene expression, leading to enhanced resistance and selection.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Cell Biology
Naohiro Kato, Gabela Nelson, Kyle J. Lauersen
Summary: Fatty acids play a crucial role in biological processes, but their metabolism in microalgae, particularly in Chlamydomonas reinhardtii, differs from animals and yeast. Recent studies on C. reinhardtii suggest that lipid turnover is mainly focused on recycling acyl chains within membranes rather than beta-oxidation for energy production. This preference for membrane turnover over beta-oxidation is highlighted through fluorescence microscopy analysis, which also reveals the detoxifying capability of the alga towards hydrogen peroxide produced during beta-oxidation.
Article
Plant Sciences
Ren Wang, Ellie Himschoot, Matteo Grenzi, Jian Chen, Alaeddine Safi, Melanie Krebs, Karin Schumacher, Moritz K. Nowack, Wolfgang Moeder, Keiko Yoshioka, Daniel Van Damme, Ive De Smet, Danny Geelen, Tom Beeckman, Jiri Friml, Alex Costa, Steffen Vanneste
Summary: This study evaluates the effects of different auxin analogs on the accumulation of PIN proteins in BFA bodies and their ability to induce Ca2+ responses. The results show that not all auxin analogs can elicit Ca2+ responses and their ability to do so partially correlates with their inhibition of BFA body formation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Limin Wang, Youzheng Ning, Jian Sun, Katie A. Wilkins, Elsa Matthus, Rose E. McNelly, Adeeba Dark, Lourdes Rubio, Wolfgang Moeder, Keiko Yoshioka, Anne-Alienor Very, Gary Stacey, Nathalie Leblanc-Fournier, Valerie Legue, Bruno Moulia, Julia M. Davies
Summary: Extracellular ATP can induce an increase in cytosolic free calcium ion as a second messenger, and CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) is identified as a critical component linking extracellular ATP receptors to calcium ion signaling in roots.
Article
Multidisciplinary Sciences
Hee Jin Kim, Naohiro Kato, Ruth Ndathe, Gregory N. Thyssen, Don C. Jones, Harish H. Ratnayaka
Summary: The study found that under optimal temperature, the im mutant displayed similar photosynthetic performance as TM-1, but under high temperature stress, the im mutant reduced photosynthesis by disproportionately decreasing stomatal conductance. Transcriptomic analysis showed differences in expression of heat stress response genes between NIL leaves, suggesting that mitochondrial complex I activity is required for optimal photosynthetic performance and adaptation to high temperature stress.
Review
Biochemistry & Molecular Biology
Guangyuan Xu, Wolfgang Moeder, Keiko Yoshioka, Libo Shan
Summary: Calcium channels play crucial roles in plant immunity by regulating calcium concentration and signaling. Different branches of the plant immune system exhibit specific calcium signatures. Studying diverse types of calcium channels helps to understand the molecular mechanisms of plant immunity.
Article
Plant Sciences
Ruth Ndathe, Renee Dale, Naohiro Kato
Summary: The ABA signaling pathway is crucial for plants to defend against drought stress. Recent research suggests that ABA alters the expression kinetics of over 6,500 genes through ABF-ABRE associations. This study constructed a model that highlights the importance of a single ABRE in the ABA gene regulatory network.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Naohiro Kato, Clayton McCuiston, Kimberly A. Szuska, Kyle J. Lauersen, Gabela Nelson, Alexis Strain
Summary: Chlamydomonas reinhardtii preferentially sequesters lipid acyl chains as a means to rapidly rebuild its membranes. It has a complete set of peroxisome biogenesis factors and can simultaneously carry diverse populations of peroxisomes, modulating their contents based on light conditions. These results support the idea that lipid biosynthesis, rather than beta-oxidation, is the primary metabolic preference for fatty acids in the alga.
Article
Multidisciplinary Sciences
Gabela Nelson, Alexis Strain, Atsuko Isu, Alireza Rahnama, Ken-ichi Wakabayashi, Adam T. T. Melvin, Naohiro Kato
Summary: The mechanisms governing chemotaxis in Chlamydomonas reinhardtii are still poorly understood. In this study, a modified Petri dish assay revealed a novel mechanism for ammonium chemotaxis in Chlamydomonas. Light exposure enhanced the chemotactic response, but phototaxis-incompetent mutant strains still exhibited normal chemotaxis. Collective migration was observed during chemotaxis but not phototaxis. The AGG1 protein was found to suppress collective migration during chemotaxis.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Sonhita Chakraborty, Masatsugu Toyota, Wolfgang Moeder, Kimberley Chin, Alex Fortuna, Marc Champigny, Steffen Vanneste, Simon Gilroy, Tom Beeckman, Eiji Nambara, Keiko Yoshioka
Summary: CNGC2 has been implicated in plant immunity and Ca2+ signaling, but also plays a role in auxin signaling regulation. Mutants of cngc2 display defects in gravitropism and auxin response, with higher levels of endogenous auxin. These phenotypes can be suppressed by mutations in auxin biosynthesis genes, suggesting a tight relationship between CNGC2 and auxin homeostasis.