Article
Plant Sciences
Guihua Wang, Weihuai Wu, Shibei Tan, Yanqiong Liang, Chunping He, Helong Chen, Xing Huang, Kexian Yi
Summary: In this study, a specific nested PCR assay for the detection of 16SrI group phytoplasma in sisal was developed, which showed high accuracy and sensitivity. This method was also used to investigate the potential vector transmission of SPLDaP by Dysmicoccus neobrevipes.
Article
Plant Sciences
Maria Elena Santos-Cervantes, Jesus Enrique Camacho-Bojorquez, Ulises Rosario Escobedo-Rivera, Jesus Mendez-Lozano, Norma Elena Leyva-Lopez
Summary: This study reported the first discovery of loofah disease in Mexico associated with phytoplasmas belonging to the 16SrI and 16SrIII groups. Phylogenetic analysis identified four new phytoplasma strains associated with loofah diseases, confirming both single and mixed infections in loofah plants and nearby weeds.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Agronomy
Guihua Wang, Weihuai Wu, Jingen Xi, Helong Chen, Chunping He, Ye LI, Jinlong Zheng, John S. Hu, Kexian Yi
Summary: Sisal purple leafroll disease (SPLD), characterized by purple margins and rolling and yellowing of the leaves, is a destructive disease affecting sisal in China. Recent findings using PCR assays and transmission electron microscopy confirm the presence of phytoplasma in SPLD-affected sisal samples. Comparison of the detection rate of phytoplasma in symptomatic and asymptomatic sisal populations showed some association between phytoplasma and SPLD. Sequencing analyses revealed the presence of phytoplasma belonging to 16SrI-B, which is the first report of 16SrI-B phytoplasma infecting sisal in China.
PLANT PROTECTION SCIENCE
(2023)
Article
Agronomy
Delia Gamarra Gamarra, Charo Milagros Villar, Gilberto Torres Suarez, Walter Darlyn Ingaruca Esteban, Nicoletta Contaldo, Emerson Clovis Carrasco Lozano, Assunta Bertaccini
Summary: Corn plants in Peru with multiple symptoms were found to be infected by 'Candidatus Phytoplasma' strains related to aster and pruni. The presence of these phytoplasmas in maize and their association with the maize bushy stunt disease provide important information for disease management.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Geon-Woo Lee, Sang -Sub Han
Summary: Phytoplasmas were found in diseased Elaeocarpus sylvestris trees on Jeju Island, showing symptoms of yellowing and darkening leaves. Two phytoplasmas, 'Candidatus Phytoplasma asteris' and 'Ca. P. malaysianum', belonging to 16SrI and 16SrXXXII groups, were detected through sequence analysis. Additionally, the presence of mixed infection and specific primer sets for distinguishing phytoplasmas were identified.
PLANT PATHOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Y. S. Shreenath, Sajad Un Nabi, G. S. Madhu, Kishan Lal Kumawat, Govind P. Rao
Summary: This study identified a common phytoplasma disease in sweet cherry cultivars in Srinagar, Jammu and Kashmir province, India, and confirmed the effectiveness of molecular markers in phytoplasma identification.
Article
Agronomy
Leila Zirak, Reza Khakvar, Gholamreza Zarrini, Karim Hasanpour
Summary: Surveys in stone fruit orchards in East Azerbaijan province of Iran from 2015 to 2018 revealed phytoplasma infections in various stone fruit trees, causing different symptoms. Molecular analyses confirmed the association of different phytoplasma species with different types of stone fruit trees.
Article
Plant Sciences
Shao-shuai Yu, Ying-wen Pan, Hui Zhu, Wei-wei Song
Summary: Coconut lethal yellowing (LY) diseases caused by phytoplasmas are devastating for the coconut industry. This study designed and screened LAMP primers and established universal detection methods for rapid diagnosis and identification of different groups of LY phytoplasmas. These methods have significant importance for efficient monitoring, early warning, and port inspection and quarantine of LY phytoplasmas and related diseases.
Article
Chemistry, Applied
Margit Drapal, Bert De Boeck, Hannele Lindqvist Kreuze, Merideth Bonierbale, Paul D. Fraser
Summary: Modern potato breeding programmes aim to develop high yielding and nutritious potatoes that meet agronomic and consumer preferences. Sensory data and metabolomic analysis have revealed the complex metabolic composition of potato tubers and its relation to flavor intensity and consumer traits.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Boris Veltman, Dorin Harpaz, Sarit Melamed, Zipora Tietel, Leah Tsror, Evgeni Eltzov
Summary: In this study, a whole-cell bacterial biosensor was developed to detect potato tuber soft rot disease. The biosensor relies on the luminescent responses of the bacteria panel to changes in the volatile organic compound (VOC) profiles following infection. The results show that this biosensor system can improve crop management during postharvest, storage, and transport to reduce food losses.
Article
Chemistry, Analytical
Usman Latif, Alexandra Seifner, Franz L. Dickert
Summary: Blood transfusion and organ transplantation require prior blood group typing and crossmatching. A mass-sensitive QCM sensor for blood group typing has been developed using molecular imprinting technology. The sensor uses two resonators with imprinted and non-imprinted polymers to measure the response to different blood group erythrocytes. The response pattern allows for clear characterization of each blood group.
Review
Biochemistry & Molecular Biology
Magdalena Skotniczny, Pawel Satora
Summary: Lactiplantibacillus plantarum is a widely distributed lactic acid bacterium with a large and flexible genome, resulting in high strain diversity. This review provides an overview of molecular techniques, both culture-dependent and culture-independent, used for the detection and identification of L. plantarum. Some of these techniques can also be applied to the analysis of other lactic acid bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Marco Chiani, Gianluigi Liva, Enrico Paolini
Summary: Group testing is a cost-effective method that allows testing pools of samples instead of individual samples. The introduced protocols can effectively identify positive samples within the first round of tests, with the option of a second round to detect multiple positives. Compared to the Dorfman's scheme, these protocols offer higher efficiency in classifying individuals with fewer tests.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Applied
Yuanmiao Wei, Ling Li, Yao Liu, Shuna Xiang, Hanyue Zhang, Lunzhao Yi, Ying Shang, Wentao Xu
Summary: Edible fungi have great potential but confusion and food-borne diseases are common due to misidentification. Different detection methods based on substances in edible fungi include sensory identification, instrumental analysis, and molecular biology identification methods. Understanding the principles and applications of these techniques is crucial for accurate identification and food safety.
Article
Agriculture, Multidisciplinary
Fatemeh Movahedi-Parizi, Akbar Hosseinipour, Mehdi Azadvar, Hossain Massumi
Summary: Phytoplasma-related symptoms were found on plants in urban green spaces in Kerman province, southeastern Iran. PCR and sequencing were used to identify the phytoplasma strains and subgroups infecting the symptomatic plants, leading to the discovery of new infections.
ANNALS OF APPLIED BIOLOGY
(2023)
Article
Plant Sciences
Robert A. Owens, Natalya V. Girsova, Ksenia A. Kromina, Ing Ming Lee, Karina A. Mozhaeva, Tatyana B. Kastalyeva
News Item
Plant Sciences
H. Khalil, R. N. Yousef, N. V. Girsova, D. Z. Bogoutdinov, T. B. Kastalyeva, S. Aldenkawe
