期刊
PLANT CELL REPORTS
卷 32, 期 3, 页码 453-463出版社
SPRINGER
DOI: 10.1007/s00299-012-1378-x
关键词
Populus; Auxin; DR5; Wood formation; Phloem; Adventitious roots
资金
- US Department of Energy (DOE), Poplar Genome Based Research for Carbon Sequestration in Terrestrial Ecosystems [DE-FG02-06ER64185, DE-FG02-05ER64113]
- Consortium for Plant Biotechnology Research, Inc. [GO12026-203A]
- United States Department of Agriculture (USDA) CSREES
- USDA-NRI Plant Genome program [2003-04345]
- USDA CSREES
- Biotechnology Risk Assessment Research Grants Program [2004-35300-14687]
- USDA [2009-65504-05767]
- DOE [2009-65504-05767]
Auxin responsive promoter DR5 reporter system is functional in Populus to monitor auxin response in tissues including leaves, roots, and stems. We described the behavior of the DR5::GUS reporter system in stably transformed Populus plants. We found several similarities with Arabidopsis, including sensitivity to native and synthetic auxins, rapid induction after treatment in a variety of tissues, and maximal responses in root tissues. There were also several important differences from Arabidopsis, including slower time to maximum response and lower induction amplitude. Young leaves and stem sections below the apex showed much higher DR5 activity than did older leaves and stems undergoing secondary growth. DR5 activity was highest in cortex, suggesting high levels of auxin concentration and/or sensitivity in this tissue. Our study shows that the DR5 reporter system is a sensitive and facile system for monitoring auxin responses and distribution at cellular resolution in poplar.
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