Article
Plant Sciences
Lin Rui, Zi-Qi Zhu, Yu-Ying Yang, Da-Ru Wang, Hao-Feng Liu, Peng-Fei Zheng, Hong-Liang Li, Guo-Dong Liu, Ran-Xin Liu, Xiaofei Wang, Shuai Zhang, Chun-Xiang You
Summary: This study identified an apple gene, MdERF113, which belongs to the AP2/ERF transcription factor family and is involved in plant growth and various biological processes. MdERF113 was found to be highly expressed in the sarcocarp and responsive to hormonal signals and abiotic stresses. Overexpression of MdERF113 in apple calli resulted in reduced sensitivity to low temperature, drought, salinity, and abscisic acid. The study also showed that MdERF113 is a nuclear-localized transcription factor with no autonomous activation activity.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Biochemistry & Molecular Biology
Shijie Ye, Dongjie Yin, Xiaoyan Sun, Qinyi Chen, Ting Min, Hongxun Wang, Limei Wang
Summary: In this study, the TbUGGT gene was cloned from Trapa bispinosa Roxb. and its functional analysis was conducted, laying the foundation for further research on the synthesis of β G in Escherichia coli.
Article
Biotechnology & Applied Microbiology
Guowei Liao, Shulan Wang, Zimo Wang, Chaonan Zhang, Zicong Li, Huirong Yang, Aiguo Zhou, Shaolin Xie, Lanfen Fan, Meifang Wang, Jixing Zou, Fang Zeng
Summary: Hepcidin is an antimicrobial peptide present in various species and plays an important role in the immune system of host organisms. In this study, the hepcidin gene (caHep) of the northern snakehead was cloned and found to exhibit broad-spectrum antibacterial activity by damaging bacterial cell membranes. Additionally, caHep was found to be involved in the innate immune response of the northern snakehead. Therefore, caHep has potential as an effective antibacterial drug against bacterial diseases in aquaculture animals.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Fisheries
Peng Liu, Wenyue Li, Zhongxiu Liang, Yanhui Cen, Xinrong Li, Peiyan Wang, Huiying Lv, Qingying Zhang, Honglin Chen, Jiang Lin
Summary: PFL-96, a novel galectin-binding lectin gene, was cloned from Pinctada fucata. The recombinant protein PFL-96 exhibited significant bioactivity in vitro, including coagulation activity, hemolytic activity, antibacterial activity, and inhibitory effects on tumor cell proliferation. These findings provide a fundamental basis for further investigation and optimization of the mechanism and structure of PFL-96.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Plant Sciences
Maryamsadat Vaziriyeganeh, Shanjida Khan, Janusz J. J. Zwiazek
Summary: In this study, the expression of aquaporins in several northern grasses with different salt tolerance was analyzed. It was found that the aquaporin PnuPIP2;2 was significantly upregulated in the halophytic grass Puccinellia nuttalliana after NaCl treatment. Functional analysis showed that PnuPIP2;2 not only facilitated water transport, but also increased Na+ uptake in yeast cells. Structural analysis revealed the unique pore characteristics of PnuPIP2;2, which may contribute to its transport properties.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Genetics & Heredity
Yunfei Mao, Yijun Yin, Xueli Cui, Haiyan Wang, XiaFei Su, Xin Qin, Yangbo Liu, Yanli Hu, Xiang Shen
Summary: Potassium channel genes were cloned from the superior apple rootstock line 12-2, and their characteristics including length, number of encoded amino acids, subcellular localization, and protein domain were analyzed. Semi-quantitative RT-PCR and qRT-PCR analyses showed high expression levels of these genes in roots and changes in their expression levels after exposure to ARD stress.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Yi Tian, Cai-Xia Zhang, Chen-Yu Zhu, Xiu-Hong An, Pei-Hua Cong
Summary: This study characterized the function of a Group II TGA gene, MdTGA2.1, in apple. MdTGA2.1 interacts with NPR1, participates in SA responses, and plays a role in stress tolerance.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Byung Ha Lee, Rui Wang, Ingrid M. Moberg, Sarah H. Reeder, Prativa Amom, Michelle H. Tan, Katelyn Amstutz, Pallavi Chandna, Adam Helton, Ekaterina P. Andrianova, Igor B. Zhulin, Anna A. Dobritsa
Summary: The study identified a new aperture factor, INP2, which along with INP1 forms a species-specific functional module that controls the formation of pollen apertures in flowering plants.
Article
Plant Sciences
Xiaona Wang, Xiaofen Chai, Beibei Gao, Cecilia Deng, Catrin S. Gunther, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: Nitrogen is essential for plant growth, but increased nitrogen fertilizer application can decrease nitrogen use efficiency and waste resources. In this study, we investigated the mechanism of nitrogen uptake in apple rootstocks under low-nitrogen stress using multi-omics approaches. We found that the transcription factor bHLH130 regulates the flavonoid pathway, thereby influencing nitrogen uptake efficiency.
Article
Plant Sciences
Huai-Na Gao, Han Jiang, Xin-Yu Lian, Jian-Ying Cui, Chun-Xiang You, Yu-Jin Hao, Yuan-Yuan Li
Summary: In this study, 26 potential LTPG genes were identified in apple using bioinformatics methods, and 20 of them were confirmed as MdLTPG family members based on subcellular localization predictions. These MdLTPG genes were induced by growth conditions and one protein was shown to interact with another protein, collectively participating in wax transport during plant growth and development.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Horticulture
Chang Jae Oh, Jin-Kyu Woo, Kyung Uk Yi, Young Chul Park, Hye-Young Lee, Minju Kim, Sukman Park, Su-Hyun Yun, Yi Lee, Hyun-Jin Kim, Kwan Jeong Song, Ho Bang Kim
Summary: Anthocyanins, major plant pigments responsible for coloration of plant tissues and organs, have significant health benefits. Transcriptional regulation of anthocyanin biosynthesis is influenced by environmental factors and controlled by MYB/bHLH/WD40 complexes. The Ruby locus, encoding a MYB transcription factor, dominantly regulates anthocyanin production in blood orange fruit. PCR-based molecular markers were established for genotyping Ruby locus alleles to aid marker-assisted selection of citrus breeding lines with high anthocyanin production.
SCIENTIA HORTICULTURAE
(2021)
Article
Plant Sciences
Ping Wang, Shixiong Lu, Wenfang Li, Zonghuan Ma, Juan Mao, Baihong Chen
Summary: MdAL4 gene was selected to improve drought tolerance in transgenic apple callus and Arabidopsis. Eleven Alfin-like genes were identified in apple, and MdAL4 exhibited high expression under drought conditions. Overexpression of MdAL4 enhanced drought tolerance in apple callus and Arabidopsis by regulating ROS-scavenging and stress-related genes.
PLANT CELL REPORTS
(2023)
Article
Plant Sciences
Sudhakar Reddy Palakolanu, Saurabh Gupta, Richa K. Yeshvekar, Navajeet Chakravartty, Sivasakthi Kaliamoorthy, Aishwarya Rajesh Shankhapal, Ashwini Soumya Vempati, Boney Kuriakose, Sivarama Prasad Lekkala, Manuel Philip, Rajadurai Chinnasamy Perumal, Vijaya Bhaskar Reddy Lachagari, Pooja Bhatnagar-Mathur
Summary: Pearl millet is an important crop known for its high tolerance to drought and heat stresses, with diverse germplasm showing significant phenotypic variation in response to abiotic stresses. This study focused on identifying the physiological responses of two pearl millet genotypes to low and high vapor pressure deficit, revealing differential expression of Pg-miRNAs and upregulation of drought-responsive miRNA targets under high VPD stress.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Li-Xian Li, Yue Fang, Dan Li, Zi-Hao Zhu, Ya Zhang, Zi-Yu Tang, Ting Li, Xue-Sen Chen, Shou-Qian Feng
Summary: This study investigates the effects of methyl jasmonate (MeJA) on ester aroma synthesis in apples, revealing that MeJA treatment significantly increases ester aroma production and upregulates several genes involved in the jasmonate pathway transduction. The transcription factor MdMYC2 and MdMYB85 directly interact with the promoter region of MdAAT1, enhancing its transcriptional activity. Therefore, this study elucidates the role of the gene module consisting of MdMYC2, MdMYB85, and MdAAT1 in mediating the effects of JA and promoting ester aroma synthesis in apples.
Article
Agriculture, Multidisciplinary
Zhang Bo, Fan Wen-min, Zhu Zhen-zhen, Wang Ying, Zhao Zheng-yang
Summary: Sugar content is associated with apple sweetness. The role of the sucrose transporter MdSUT2.1 in sucrose accumulation in apples was investigated in this study. MdSUT2.1 gene was highly expressed in fruit and positively correlated with sucrose accumulation during apple fruit development. Overexpression of MdSUT2.1 in apples and tomatoes resulted in increased sucrose, fructose, and glucose contents, as well as the expression levels of sugar metabolism-and transport-related genes compared to the wild type.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Plant Sciences
Li-Jie Zhou, Ke Mao, Yu Qiao, Han Jiang, Yuan-Yuan Li, Yu-Jin Hao
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2017)
Article
Plant Sciences
Chun-Ling Zhang, Ke Mao, Li-Jie Zhou, Gui-Luan Wang, Ya-Li Zhang, Yuan-Yuan Li, Yu-Jin Hao
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Plant Sciences
Rui-Fen Zhang, Li-Jie Zhou, Yuan-Yuan Li, Chun-Xiang You, Guang-Li Sha, Yu-Jin Hao
JOURNAL OF PLANT PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Xian-Yan Zhao, Chen-Hui Qi, Han Jiang, Peng-Fei Zheng, Ming-Shuang Zhong, Qiang Zhao, Chun-Xiang You, Yuan-Yuan Li, Yu-Jin Hao
Article
Plant Sciences
Peng-Liang Han, Yuan-Hua Dong, Kai-Di Gu, Jian-Qiang Yu, Da-Gang Hu, Yu-Jin Hao
Article
Plant Sciences
Ya-Li Zhang, Chun-Ling Zhang, Gui-Luan Wang, Yong-Xu Wang, Chen-Hui Qi, Chun-Xiang You, Yuan-Yuan Li, Yu-Jin Hao
Article
Biochemistry & Molecular Biology
Xian-Yan Zhao, Chen-Hui Qi, Han Jiang, Ming-Shuang Zhong, Qiang Zhao, Chun-Xiang You, Yuan-Yuan Li, Yu-Jin Hao
MOLECULAR PLANT-MICROBE INTERACTIONS
(2019)
Article
Plant Sciences
Chun-Ling Zhang, Gui-Luan Wang, Ya-Li Zhang, Xing Hu, Li-Jie Zhou, Chun-Xiang You, Yuan-Yuan Li, Yu-Jin Hao
Summary: The apple SUMO E3 ligase MdSIZ1 promotes lateral root formation in plants by enhancing the conjugating enzyme activity of MdSCE1 to form a complex with MdARF8, facilitating SUMO modification. Mutations at K342 and K380 in MdARF8 were found to block SUMOylation, revealing a crucial role for sumoylation in the regulation of lateral root formation.
Article
Plant Sciences
Yu-Ying Yang, Peng-Fei Zheng, Yi-Ran Ren, Yu-Xin Yao, Chun-Xiang You, Xiao-Fei Wang, Yu-Jin Hao
Summary: This study identified a new bHLHm1 transcription factor MdSAT1 which functions in mediating tolerance to salt and drought resistance. Overexpression of MdSAT1 in apple calli and Arabidopsis resulted in increased tolerance to salt and drought stress. These findings pave the way for further functional investigation on MdSAT1's role in abiotic stress tolerance.
Article
Plant Sciences
Han Jiang, Qi-Jun Ma, Ming-Shuang Zhong, Huai-Na Gao, Yuan-Yuan Li, Yu-Jin Hao
Summary: In this study, MdPAT16 was identified as a key player in mediating salt stress response in apple plants through palmitoylation of target proteins. Overexpression of MdPAT16 improved plant salt stress resistance and promoted the accumulation of soluble sugars. Furthermore, MdPAT16 was shown to interact with MdCBL1 and stabilize its protein levels through palmitoylation, suggesting a potential missing link in the plant salt stress response pathway.
Article
Plant Sciences
Xin-Yu Lian, Huai-Na Gao, Han Jiang, Chang Liu, Yuan-Yuan Li
Summary: The apple wax-related gene MdKCS2 plays a role in enhancing plant resistance to drought stress by increasing wax accumulation and changing epidermal permeability. Transgenic analysis indicated that MdKCS2 improved resistance to abiotic stress in apple calli. Ectopic expression of MdKCS2 in Arabidopsis increased wax content in leaves and stems, changed cuticle permeability, and enhanced drought resistance.
PLANT CELL REPORTS
(2021)
Article
Plant Sciences
Ya-Li Zhang, Yi Tian, Yao-Yang Man, Chun-Ling Zhang, Yi Wang, Chun-Xiang You, Yuan-Yuan Li
Summary: SUMOylation plays a key role in optimizing plant growth and resistance to environmental stress. This study demonstrates that MdSIZ1 mediates the SUMOylation and accumulation of MdMYB30, leading to the activation of MdKCS1 expression and promotion of cuticular wax biosynthesis in apple.
Article
Plant Sciences
Hui Kang, Ting-Ting Zhang, Yuan-Yuan Li, Kui Lin-Wang, Richard Espley, Yuan-Peng Du, Qing-Mei Guan, Feng-Wang Ma, Yu-Jin Hao, Chun-Xiang You, Xiao-Fei Wang
Summary: The study reveals that MdBT2 interacts with and stabilizes MdCOP1 protein in apple, leading to repression of anthocyanin biosynthesis. MdBT2 enhances the ubiquitination and degradation of the transcription factor MdMYB1 mediated by MdCOP1, resulting in the inhibition of anthocyanin biosynthesis gene expression and anthocyanin accumulation.
Article
Plant Sciences
Han Jiang, Li-Jie Zhou, Huai-Na Gao, Xiao-Fei Wang, Zhi-Wen Li, Yuan-Yuan Li
Summary: This study reveals the importance of transcriptional regulation of MdSIZ1 in plant cold tolerance and anthocyanin accumulation and investigates the regulatory mechanisms of MdSIZ1.
Article
Plant Sciences
Chenhui Qi, Han Jiang, Xianyan Zhao, Ke Mao, Haitao Liu, Yuanyuan Li, Yujin Hao
HORTICULTURAL PLANT JOURNAL
(2019)