Article
Plant Sciences
Luca Capriotti, Angela Ricci, Barbara Molesini, Bruno Mezzetti, Tiziana Pandolfini, Irene Piunti, Silvia Sabbadini
Summary: Plant genetic transformation is a powerful breeding tool for improving disease tolerance, stress resistance, fruit production, and quality in fruit trees. This study validated the use of cotyledons and hypocotyls derived from somatic embryos as starting explants for in vitro regeneration and transformation trials in grapevines. The optimized protocols resulted in successful generation of transgenic plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Arleen Rodriguez-Declet, Antonio Castro-Marin, Luca Moretti Conti, Alessandra Lombini, Fabio Chinnici, Adamo Domenico Rombola
Summary: Volatile organic compounds (VOCs) are important for communication among plants and other organisms. This study identified 25 VOCs emitted by the grapevine cv. Isabella and revealed differences in volatile profiles between diurnal and nocturnal treatments. The presence of previously unreported compounds and their role in plant defense highlight the importance of this research in understanding pathogen tolerance mechanisms and exploring innovative cropping systems.
Article
Agriculture, Multidisciplinary
Alexander A. Tkachenko, Maria S. Gancheva, Varvara E. Tvorogova, Lavrenty G. Danilov, Alexander V. Predeus, Irina E. Dodueva, Ludmila L. Lutova
Summary: Using RNA-seq, gene expression changes during early stages of crown gall development in radish were analyzed, revealing different pathways upregulated or downregulated in spontaneous tumorous radish compared to non-tumorous lines.
ANNALS OF APPLIED BIOLOGY
(2021)
Article
Plant Sciences
Akira Kawaguchi, Namiko Kirino, Koji Inoue
Summary: Grapevine crown gall, caused by Allorhizobium vitis, is a significant disease worldwide. Four nonpathogenic A. vitis strains, VAR03-1, ARK-1, ARK-2, and ARK-3, have shown promise as biological control agents, but their control effects have not been directly compared. This study analyzed 16 field trials conducted between 2006 and 2017 to evaluate the control effects using a linear mixed model and network meta-analysis. The results suggest that the strain ARK-1 was the most effective antagonist against grapevine crown gall.
Article
Multidisciplinary Sciences
Haifa Ben Gharsa, Meriam Bouri, Amira Mougou Hamdane, Christina Schuster, Andreas Leclerque, Ali Rhouma
Summary: The study identifies a potential biological agent, B. velezensis MBY2, that can effectively suppress pathogenic Agrobacterium in plants, significantly reducing pathogen populations in maize and tomato plants. Through experiments, the strain demonstrates antagonistic effects in vitro and in vivo, effectively reducing gall development induced by the pathogen.
Article
Agronomy
Julia B. Rasera, Beatriz Appezzato-da-Gloria, Rafael V. Ribeiro, Antonio F. Nogueira-Junior, Lilian Amorim
Summary: Asian grapevine leaf rust (AGLR), caused by Neophysopella tropicalis, is a problem for viticulture, especially in latitudes lower than 25 degrees S, which include the most significant production regions in Brazil. Climate change has a significant impact on AGLR, as increased air temperature can lead to higher rust severity and accelerated leaf senescence in grapevines.
Article
Plant Sciences
Diana Marin, Carlos Miranda, Francisco Javier Abad, Jorge Urrestarazu, Blanca Mayor, Ana Villa-Llop, Luis Gonzaga Santesteban
Summary: Choosing the right rootstock is crucial for the profitability of vineyards. However, rootstock-breeding programs have been limited in recent years, resulting in the use of century-old rootstocks that may not be suitable for today's needs. This study evaluated eight new rootstock candidates, resulting from cross-pollination, for their agronomic performance in a vineyard in Spain. The candidates showed a wide range of performance compared to their parents, and two promising candidates (RG8 and RG10) have already started the process of becoming commercial rootstocks.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Agronomy
Rong Xiao, Xiao-Peng Mu, Jian-Cheng Zhang, Shuai Zhang, Chun-Fen Zhang, Shu Deng, Jun-Jie Du, Peng-Fei Wang
Summary: This study aimed to isolate and identify the pathogens causing crown gall disease in Prunus humilis, as well as study their physiological, phylogenetic, opine type, and biological control characteristics. The major pathogenic strains were identified as Rhizobium rhizogenes and Agrobacterium tumefaciens. Some strains possessed genes related to the traditional opines. However, the current biological control strains showed limited efficacy against the pathogenic strains derived from P. humilis.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Ignacio Buesa, Jose M. Escalona, Ignacio Tortosa, Diana Marin, Maite Loidi, Luis G. Santesteban, Cyril Douthe, Hipolito Medrano
Summary: The study showed significant differences in water use efficiency among 13 Grenache grape genotypes at different levels, with high consistency among levels despite large interannual variability. Using genotype-specific regressions allows for significant differences in water use efficiency responses to be identified, and the relationship between net photosynthesis, stomatal conductance, and carbon isotope discrimination were confirmed as reliable physiological indicators for selecting grapevine genotypes under future environmental conditions.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Jie Bai, Tuo Yao, Xiaojun Lan, Yanshan Yang, Zhanjun Wang, Xinyou Wang
Summary: The Chinese dwarf cherry crown gall disease was identified in a nursery base in Ningxia Hui Autonomous Region, China, with a pathogen identified as A. tumefaciens/ biovar 1. The pathogenicity assay showed crown gall symptoms on tomato and sunflower stems after inoculation.
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Trong Nguyen-Huu, Jeanne Dore, Essaid Ait Barka, Celine Lavire, Christophe Clement, Ludovic Vial, Lisa Sanchez
Summary: An efficient DNA-based real-time PCR assay was developed to monitor the development of Allorhizobium vitis in grapevine plantlets. The novel technique targets chromosomal genes and the virulent tumor-inducing plasmid of the pathogen, providing reliable and reproducible quantification from early infection to tumor formation in plants. This low-cost method is suitable for fundamental research and offers a rapid and robust quantification of the pathogen in planta.
Article
Horticulture
Milena Trajkovic, Milan Dragicevic, Ana D. Simonovic, Angelina R. Subotic, Snezana Milosevic, Aleksandar Cingel, Sladana Jevremovic
Summary: Flower color is crucial for determining the commercial value of ornamental plants, and modern biotechnology methods enable the creation of new colors that cannot be achieved through classical methods. Successful Agrobacterium-mediated genetic transformation of Viola cornuta Lutea Splendens resulted in changing flower color from yellow to different shades of orange.
Article
Plant Sciences
Michele Faralli, Stefania Pilati, Massimo Bertamini
Summary: The balance between vegetative and reproductive growth in grapevines is crucial for grape quality and productivity. Field experiments showed that a decrease in sink availability significantly affected the physiological functionality of leaves, but only minimal effects were observed when only the grape bunches were removed.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Microbiology
Hangwei Xi, Maarten Ryder, Iain R. Searle
Summary: The study presents the annotated, near-complete genome sequence of the phytopathogenic Rhizobiales strain Allorhizobium vitis K377, isolated from a grapevine in South Australia. The genome sequence includes various protein-coding sequences, functional genes, with potential agricultural applications.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Agriculture, Multidisciplinary
Serah Lim, Se Chul Chun, Jin-Won Kim
Summary: This study evaluated the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease. It found that 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to the disease. The study also discovered a strong correlation between the gall formation rate and the initial gall formation period.
PLANT PATHOLOGY JOURNAL
(2023)
Article
Microbiology
Veronika Deak, Rita Lukacs, Zsuzsanna Buzas, Adrienn Palvoelgyi, Peter P. Papp, Laszlo Orosz, Peter Putnoky
JOURNAL OF BACTERIOLOGY
(2010)
Article
Microbiology
Toshio Yamaguchi, Fuminori Tsutsumi, Peter Putnoky, Masahiro Fukuhara, Tatsunosuke Nakamura
Article
Biochemistry & Molecular Biology
Adrienn Palvoelgyi, Veronika Deak, Verena Poinsot, Tibor Nagy, Enik Nagy, Ildiko Kerepesi, Peter Putnoky
MOLECULAR PLANT-MICROBE INTERACTIONS
(2009)
Article
Agronomy
Anett Kuczmog, Aniko Galambos, Szabina Horvath, Aniko Matai, Pal Kozma, Erno Szegedi, Peter Putnoky
THEORETICAL AND APPLIED GENETICS
(2012)
Article
Virology
Brigitta Zana, Karoly Erdelyi, Anna Nagy, Eszter Mezei, Orsolya Nagy, Maria Takacs, Tamas Bakonyi, Petra Forgach, Orsolya Korbacska-Kutasi, Orsolya Feher, Peter Malik, Krisztina Ursu, Peter Kertesz, Anett Kepner, Mate Martina, Tamas Suli, Zsofia Lanszki, Gabor Endre Toth, Anett Kuczmog, Balazs Somogyi, Ferenc Jakab, Gabor Kemenesi
Article
Infectious Diseases
Erik Alm, Eeva K. Broberg, Thomas Connor, Emma B. Hodcroft, Andrey B. Komissarov, Sebastian Maurer-Stroh, Angeliki Melidou, Richard A. Neher, Aine O'Toole, Dmitriy Pereyaslov
Article
Chemistry, Medicinal
Ilona Bereczki, Henrietta Papp, Anett Kuczmog, Monika Madai, Veronika Nagy, Attila Agocs, Gyula Batta, Marton Milankovits, Eszter Ostorhazi, Janko Kos, Aron Zsigmond, Istvan Hajdu, David Bajusz, Jan Hodek, Jan Weber, Ferenc Jakab, Pal Herczegh, Aniko Borbas, Ana MitroviC, Zsolt Lorincz, Gyorgy Miklos Keserii
Summary: The prolonged global pandemic of COVID-19 has driven the need for the development of new drugs targeting SARS-CoV-2, with the glycopeptide antibiotic teicoplanin and certain apocarotenoids showing promising antiviral potential. The study suggests that these compounds could be further explored as potential antivirals against SARS-CoV-2.
Article
Biochemical Research Methods
Szilvia Barsi, Henrietta Papp, Alberto Valdeolivas, Daniel J. Toth, Anett Kuczmog, Monika Madai, Laszlo Hunyady, Peter Varnai, Julio Saez-Rodriguez, Ferenc Jakab, Bence Szalai
Summary: Comparing gene expression signatures of SARS-CoV-2 infection and drug treatment can help repurpose existing drugs against SARS-CoV-2 and identify key mechanisms behind their antiviral effect.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Sung-Ting Chuang, Henrietta Papp, Anett Kuczmog, Rebecca Eells, Jose M. Condor Capcha, Lina A. Shehadeh, Ferenc Jakab, Peter Buchwald
Summary: Methylene blue has been identified as an inhibitor of the protein-protein interaction between the spike protein of SARS-CoV-2 and ACE2, and it can effectively inhibit the entry and replication of the virus. It also shows activity against other viral proteins and exhibits antiviral potency in vitro.
Article
Multidisciplinary Sciences
Jinge Liu, Ting Wang, Qiulin Qin, Xiaocheng Yu, Shengming Yang, Randy D. Dinkins, Anett Kuczmog, Peter Putnoky, Artur Muszynski, Joel S. Griffitts, Attila Kereszt, Hongyan Zhu
Summary: Plants have evolved the ability to distinguish between symbiotic and pathogenic microbial signals. The NS1 locus in Medicago truncatula can block the invasion and nodule induction by Sinorhizobium meliloti. The NS1 locus is controlled by two genes, NS1a and NS1b, which can regulate the nodulation blockade of specific bacterial strains.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
M Putnoky, V Deák, K Békási, A Pálvölgyi, A Maász, Z Palágyi, G Hoffmann, I Kerepesi
JOURNAL OF BACTERIOLOGY
(2004)
Article
Biochemistry & Molecular Biology
E Kiss, A Kereszt, F Barta, S Stephens, BL Reuhs, A Kondorosi, P Putnoky
MOLECULAR PLANT-MICROBE INTERACTIONS
(2001)
