Article
Plant Sciences
Lei Yan, Liu Zeng, Ali Raza, Yan Lv, Xiaoyu Ding, Yong Cheng, Xiling Zou
Summary: This study aimed to investigate the beneficial role of exogenous inositol in improving cold stress tolerance in rapeseed. The results revealed that inositol positively regulated cold tolerance by increasing calcium ion influx in rapeseed. The inositol-Ca2+ pathway was found to play a key role in cold tolerance. This study provides new insights into the mechanism of rapeseed cold tolerance and introduces a feasible method for improving rapeseed cold tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Ali Raza, Wei Su, Ziqi Jia, Dan Luo, Yi Zhang, Ang Gao, Muhammad Azhar Hussain, Sundas Saher Mehmood, Yong Cheng, Yan Lv, Xiling Zou
Summary: Trehalose acts as a growth modulator and improves cold stress tolerance in rapeseed seedlings. The study found that exogenous trehalose mitigates the adverse effects of cold stress on rapeseed seedlings, increasing their survival rate. Trehalose regulates antioxidant and osmotic balance, participates in metabolism and signaling networks to enhance cold stress tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Wenbo Mi, Zigang Liu, Jiaojiao Jin, Xiaoyun Dong, Chunmei Xu, Ya Zou, Mingxia Xu, Guoqiang Zheng, Xiaodong Cao, Xinling Fang, Caixia Zhao, Chao Mi
Summary: The study compared two winter rapeseed cultivars, NS and NF, under cold stress and found that NS exhibited higher antioxidant activity and osmotic adjustment ability, and less plasma membrane injury compared to NF. Proteomic analysis revealed that NS accumulated proteins related to ROS scavenging more rapidly in lysine degradation, phenylalanine, tyrosine, and tryptophan, flavonoid biosynthesis, and ubiquinone and other terpenoid-quinone biosynthesis pathways, enhancing cold tolerance and minimizing ROS damage.
Article
Plant Sciences
Guoqiang Zheng, Xiaoyun Dong, Jiaping Wei, Zigang Liu, Ali Aslam, JunMei Cui, Hui Li, Ying Wang, Haiyan Tian, Xiaodong Cao
Summary: In this study, the DNA methylation pattern and its regulated pathway of winter rapeseed under freezing stress were explored using methylome and transcriptome analysis. The results showed that the average methylation level decreased under freezing stress, with CG methylation level being the highest. Functional enrichment analysis identified several differentially expressed genes associated with differentially methylated genes, including core transcription factors involved in plant response to stress.
Article
Plant Sciences
Zigang Liu, Xiaoyun Dong, Xiaodong Cao, Chunmei Xu, Jiaping Wei, Guoqiang Zhen, Jinxiong Wang, Hui Li, Xinlin Fang, Yin Wang, Hai Yan, Chao Mi, Caixia Zhao, Wenbo Mi
Summary: This study identified the genetic mechanism underlying the cold tolerance of winter rapeseed (Brassica napus) and discovered QTLs and candidate genes related to winter survival. These results are important for improving the cold tolerance and overwintering success of winter oilseed rape.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Genetics & Heredity
Tao Xie, Wenjing Yang, Xin Chen, Hao Rong, Youping Wang, Jinjin Jiang
Summary: Through bioinformatic analyses, researchers identified 33 BnMTP genes in rapeseed, which were classified into three major clusters and seven groups. Evolutionary analysis indicated that the BnMTP gene family mainly expanded through whole-genome duplication and segmental duplication events. Predictions suggested that BnMTPs might be involved in plant growth, development, and stress responses.
Article
Plant Sciences
Pengfei Cheng, Liying Feng, Shuoyu Zhang, Longna Li, Rongzhan Guan, Weihua Long, Zhihui Xian, Jiefu Zhang, Wenbiao Shen
Summary: This study investigated the role of ammonia borane (AB), a hydrogen (H-2) donor, in enhancing rapeseeds' tolerance against cold stress. Results showed that application of AB alleviated the inhibition of seedling growth and reduced oxidative damage caused by cold stress. The increased tolerance was attributed to the enhanced antioxidant enzyme system and restored redox homeostasis, as well as the stimulation of endogenous hydrogen sulfide (H2S) biosynthesis. Field experiments further demonstrated the improved performance of rapeseed plants challenged by cold stress after AB administration. The study suggests that AB could be a suitable option for enhancing cold tolerance in field agriculture.
Article
Biochemical Research Methods
Aruni Y. Alahakoon, Eden Tongson, Wei Meng, Zi-Wei Ye, Derek A. Russell, Mee-Len Chye, John F. Golz, Paul W. J. Taylor
Summary: This study developed an in-vitro regeneration protocol for rapid-cycling Brassica napus (B. napus-RC) using cotyledons as the explant. High regeneration percentages were achieved by including specific growth regulators in the regeneration medium. Transformation of B. napus-RC was successfully carried out using Agrobacterium-mediated transformation, resulting in transgenic plants overexpressing AtACBP6. These transgenic plants exhibited improved cold tolerance and higher yields compared to the wild type. The results suggest that B. napus-RC can serve as an effective trait testing platform for canola, and the overexpression of AtACBP6 shows potential for enhancing cold tolerance in canola.
Article
Biochemistry & Molecular Biology
Xinhong Liu, Ran Wei, Minyu Tian, Jinchu Liu, Ying Ruan, Chuanxin Sun, Chunlin Liu
Summary: The study explored the transcriptome and metabolome profiles of two rapeseed genotypes with different cold responses, revealing differential accumulation of metabolites and gene expression related to sugar metabolism, biosynthesis, photosynthesis, and MAPK signaling in response to cold stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dan Luo, Ali Raza, Yong Cheng, Xiling Zou, Yan Lv
Summary: In this study, it was found that the transcript level of BnaMYBL17 was induced by cold stress in rapeseed. Functional analysis revealed that overexpression of BnaMYBL17 increased sensitivity to freezing stress. Transcriptionomic analysis identified 14,298 differentially expressed genes and 1321 candidate target genes related to freezing response. Verification experiments confirmed that BnaMYBL17 affects the promoter of BnaPLC1, BnaFLZ8, and BnaKOIN genes. Overall, BnaMYBL17 acts as a transcriptional repressor in regulating certain genes related to growth and development during freezing stress, providing valuable targets for molecular breeding to enhance freezing tolerance in rapeseed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Diana Saja-Garbarz, Agnieszka Ostrowska, Katarzyna Kaczanowska, Franciszek Janowiak
Summary: The study found that silicon accumulates even in non-accumulator species like oilseed rape under certain conditions, and potentially improves water uptake under drought stress.
Article
Plant Sciences
Ka Zhang, Fei Liu, Zhixin Wang, Chenjian Zhuo, Kaining Hu, Xiaoxia Li, Jing Wen, Bin Yi, Jinxiong Shen, Chaozhi Ma, Tingdong Fu, Jinxing Tu
Summary: In this study, it was found that oilseed rape enhances its resistance against Sclerotinia infection through a conserved MAPK cascade-mediated phosphorylation of BnWRKY33. Additionally, the induction of BnaA03.WRKY28 and BnaA09.VQ12 in the later stages of infection suppresses BnWRKY33 expression but promotes branching growth. These findings highlight the adaptability of oilseed rape in response to lethal pathogens, as it modulates its defense-response strength while balancing growth.
Article
Plant Sciences
Yi Zhang, Ali Raza, He Huang, Wei Su, Dan Luo, Liu Zeng, Xiaoyu Ding, Yong Cheng, Zhaofeng Liu, Quanan Li, Yan Lv, Xiling Zou
Summary: In this study, 35 Lhcb genes (BnLhcbs) were identified in the rapeseed genome, which play essential roles in photosynthesis and stress response. The BnLhcb3.4 gene was significantly induced in the tolerant rapeseed cultivar and down-regulated in the sensitive cultivar. Overexpression of BnLhcb3.4 gene enhanced the freezing tolerance of transgenic Arabidopsis and altered the expression of ABA-responsive genes.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Jingdong Chen, Heping Wan, Wenhui Zhu, Xigang Dai, Yi Yu, Changli Zeng
Summary: By analyzing the expression of the BnIPT gene family in Brassica napus under different exogenous hormones and abiotic stress treatments, this study provides a theoretical basis for understanding the function and molecular genetic mechanisms of the BnIPT gene family in nitrogen deficiency stress tolerance in rapeseed.
Article
Plant Sciences
Xingzhi Qian, Wenyin Zheng, Jian Hu, Jinxu Ma, Mengyuan Sun, Yong Li, Nian Liu, Tianhua Chen, Meiqi Wang, Ling Wang, Xinzhe Hou, Qingao Cai, Zhaoshun Ye, Fugui Zhang, Zonghe Zhu
Summary: In this study, 26 BnDFR genes were identified and analyzed for their physicochemical properties, subcellular localization, and conserved structure. The evolutionary relationship, collinearity analysis, and expression characteristics of these genes were studied, as well as the correlation between their expression levels and anthocyanin content in rape petals. The results showed that these genes were distributed in different subcellular compartments, chromosomes, and groups, and certain genes may play a key role in anthocyanin synthesis in rape petals.