期刊
PLANT CELL AND ENVIRONMENT
卷 35, 期 2, 页码 360-373出版社
WILEY
DOI: 10.1111/j.1365-3040.2011.02355.x
关键词
Arabidopsis; iron-sulphur
资金
- Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche [ANR-Blanc-10 FIRES]
The major known function of glutaredoxins (Grxs) is to reduce disulphide bridges. Recently, some have also been shown to interact with ironsulphur clusters. These can be classified in two subgroups: class II Grx are found in all living organisms and are implicated in assembly of ironsulphur clusters, while class I Grx are represented by only two members known to form a holodimer structure containing a cluster in vitro, but with an unknown function different from class II. Here, we report that in eukaryotic plants, GRXC1 (class I) orthologs are exclusively present in dicotyledonous plants, suggesting a specific function. Indeed, in Arabidopsis thaliana, reducing activity of recombinant GRXC1 is regulated by redox-dependent stability of the cluster. In planta, GRXC1 has been found predominantly in a holodimeric form, indicating the presence of the cluster in vivo. This suggests that GRXC1 acts as a redox sensor, reducing downstream pathways under oxidative conditions. GRXC2, the closest homolog of GRXC1, is unable to form a cluster in vitro. Knock-out mutants in grxc1 or grxc2 are aphenotypic, but the double mutant produces a lethal phenotype at an early stage after pollinization, suggesting that GRXC1 and GRXC2 share redundant and vital functions.
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