期刊
PLANT CELL AND ENVIRONMENT
卷 35, 期 4, 页码 668-681出版社
WILEY
DOI: 10.1111/j.1365-3040.2011.02443.x
关键词
conductance; G-proteins; oxidative stress; ozone; peroxidase; photosynthesis; ROS; stomata
资金
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0723515] Funding Source: National Science Foundation
- NIGMS NIH HHS [R01 GM065989] Funding Source: Medline
Ozone (O3) uptake by plants leads to an increase in reactive oxygen species (ROS) in the intercellular space of leaves and induces signalling processes reported to involve the membrane-bound heterotrimeric G-protein complex. Therefore, potential G-protein-mediated response mechanisms to O3 were compared between Arabidopsis thaliana L. lines with null mutations in the alpha- and beta-subunits (gpa1-4, agb1-2 and gpa1-4/agb1-2) and Col-0 wild-type plants. Plants were treated with a range of O3 concentrations (5, 125, 175 and 300 nL L-1) for 1 and 2 d in controlled environment chambers. Transcript levels of GPA1, AGB1 and RGS1 transiently increased in Col-0 exposed to 125 nL L-1 O3 compared with the 5 nL L-1 control treatment. However, silencing of a and beta G-protein genes resulted in little alteration of many processes associated with O3 injury, including the induction of ROS-signalling genes, increased leaf tissue ion leakage, decreased net photosynthesis and stomatal conductance, and increased peroxidase activity, especially in the leaf apoplast. These results indicated that many responses to O3 stress at physiological levels were not detectably influenced by a and beta G-proteins.
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