期刊
PLANT CELL
卷 26, 期 11, 页码 4499-4518出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.114.129965
关键词
-
资金
- Office of Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division, U.S. Department of Energy [DE-FG02-12ER16338, DE-FG02-12ER16339]
- National Science Foundation Award [0920274]
- South Carolina Experiment Station Project [SC-1700340]
- National Science Foundation [MCB0824469, MCB0235878]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0920274] Funding Source: National Science Foundation
Chlamydomonas reinhardtii insertion mutants disrupted for genes encoding acetate kinases (EC 2.7.2.1) (ACK1 and ACK2) and a phosphate acetyltransferase (EC 2.3.1.8) (PAT2, but not PAT1) were isolated to characterize fermentative acetate production. ACK1 and PAT2 were localized to chloroplasts, while ACK2 and PAT1 were shown to be in mitochondria. Characterization of the mutants showed that PAT2 and ACK1 activity in chloroplasts plays a dominant role (relative to ACK2 and PAT1 in mitochondria) in producing acetate under dark, anoxic conditions and, surprisingly, also suggested that Chlamydomonas has other pathways that generate acetate in the absence of ACK activity. We identified a number of proteins associated with alternative pathways for acetate production that are encoded on the Chlamydomonas genome. Furthermore, we observed that only modest alterations in the accumulation of fermentative products occurred in the ack1, ack2, and ack1 ack2 mutants, which contrasts with the substantial metabolite alterations described in strains devoid of other key fermentation enzymes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据