Article
Biochemistry & Molecular Biology
Jana Knoppova, Jianfeng Yu, Jan Janouskovec, Petr Halada, Peter J. Nixon, Julian P. Whitelegge, Josef Komenda
Summary: Photochemical energy conversion in oxygenic photosynthesis relies on membrane-embedded chlorophyll-binding protein complexes, which are regulated by lipoproteins such as Ycf48. Ycf48, a lipoprotein assembly factor in cyanobacteria, plays a crucial role in the biogenesis and function of the photosystem II complex. The unique lipobox sequence in Ycf48 distinguishes it from canonical lipobox sequences and highlights its importance in cyanobacterial PSII assembly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ying-Qi Wang, Jing-Jing Ye, Hong-Zhiyuan Yang, Da Li, Xiao-Xiang Li, Yong-Kang Wang, Xin-Qiang Zheng, Jian-Hui Ye, Qing-Sheng Li, Yue-Rong Liang, Jian-Liang Lu
Summary: The light-sensitive albino tea plant can gradually recover its photosynthetic pigments, chloroplast ultrastructure, and photosynthesis parameters from pale yellow to green through short-term shading treatment. This is attributed to the proper formation of pigment-protein complexes on the thylakoid membrane, benefiting from increased levels of LHCII subunits, particularly the deficiency of Lhcb1, in the shaded leaves of 'HJY'.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Michela Cecchin, Jooyeon Jeong, Woojae Son, Minjae Kim, Seunghye Park, Luca Zuliani, Stefano Cazzaniga, Andrea Pompa, Chan Young Kang, Sangsu Bae, Matteo Ballottari, EonSeon Jin
Summary: This study generated lpa2 knockout mutants in Chlamydomonas reinhardtii using the CRISPR-Cas9 system and found that the absence of LPA2 protein resulted in impaired PSII assembly and function, leading to compromised photoautotrophic growth.
Article
Biochemistry & Molecular Biology
Ying Chen, Wataru Yamori, Ayumi Tanaka, Ryouichi Tanaka, Hisashi Ito
Summary: During leaf senescence, the degradation of photosystems and photosynthetic pigments proceeds in a coordinated manner, with the degradation of photosystem II core complexes regulated independently of the major chlorophyll degradation pathway. The study provides insights into the complex molecular mechanisms underlying the degradation of photosystems, which is an essential step during leaf senescence.
Article
Plant Sciences
Hanaki Maeda, Koharu Takahashi, Yoshifumi Ueno, Kei Sakata, Akari Yokoyama, Kozue Yarimizu, Fumiyoshi Myouga, Kazuo Shinozaki, Shin-Ichiro Ozawa, Yuichiro Takahashi, Ayumi Tanaka, Hisashi Ito, Seiji Akimoto, Atsushi Takabayashi, Ryouichi Tanaka
Summary: The assembly process of PSII in plants requires various auxiliary proteins to form intermediates, including OHP1, OHP2, and HCF244 proteins along with D1, D2 subunits. Analysis of the OHP1 complexes revealed their composition and ability to bind chlorophylls and carotenoids for quenching. These auxiliary proteins play a crucial role in acclimating plants to high-light conditions by maintaining the yields of PSII.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Multidisciplinary Sciences
Omar Sandoval-Ibanez, David Rolo, Rabea Ghandour, Alexander P. Hertle, Tegan Armarego-Marriott, Arun Sampathkumar, Reimo Zoschke, Ralph Bock
Summary: The DEIP1 protein interacts with the PetA and PetB subunits and plays an essential role in the assembly of the Cytb6f complex. This finding provides insights into the assembly pathway of a crucial complex in photosynthesis.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Guillem Pascual-Aznar, Grzegorz Konert, Martina Beckova, Eva Kotabova, Zdenko Gardian, Jana Knoppova, Lenka Bucinska, Radek Kana, Roman Sobotka, Josef Komenda
Summary: Photosystem II (PSII) is a large membrane protein complex involved in primary charge separation in oxygenic photosynthesis. The assembly of PSII involves different modules that contain chlorophyll-binding proteins, membrane polypeptides, pigments and cofactors. Psb35 protein, identified in the CP47 antenna module, plays a crucial role in the accumulation and stability of chlorophyll-binding proteins.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Lulu Ren, Hongping Ma, Donghui Chao, Haizhen Zhang, Kun Qiao, Shuang Feng, Aimin Zhou
Summary: In this study, the functions of two Arabidopsis (Arabidopsis thaliana) Sep proteins, Sep1 and Sep2, in Chl breakdown and in response to light stress were investigated and compared. It was found that Sep2 reduced the levels of Chl by promoting Chl breakdown and could alleviate the accumulation of reactive oxygen species under long-term light stress. However, Sep1 did not cause Chl breakdown in Arabidopsis leaves. These findings suggest that Sep2, as a member of the LHC-like family, might regulate the levels of free Chl by binding to Chl and participate in light-stress response.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Jure Zabret, Stefan Bohn, Sandra K. Schuller, Oliver Arnolds, Madeline Moller, Jakob Meier-Credo, Pasqual Liauw, Aaron Chan, Emad Tajkhorshid, Julian D. Langer, Raphael Stoll, Anja Krieger-Liszkay, Benjamin D. Engel, Till Rudack, Jan M. Schuller, Marc M. Nowaczyk
Summary: Auxiliary proteins play a crucial role in assisting the assembly of photosystems, as shown by the structure of a PSII assembly intermediate resolved by cryo-electron microscopy. This structure reveals mechanisms that protect against photodamage during vulnerable stages of biogenesis and prepare the active site for the unique water-splitting reaction catalyzed by the Mn4CaO5 cluster.
Article
Multidisciplinary Sciences
Christopher J. Gisriel, Jimin Wang, Jinchan Liu, David A. Flesher, Krystle M. Reiss, Hao-Li Huang, Ke R. Yang, William H. Armstrong, M. R. Gunner, Victor S. Batista, Richard J. Debus, Gary W. Brudvig
Summary: The cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 has been determined at high resolution, revealing differences from thermophilic cyanobacterial PSII structures that significantly impact the understanding of PSII structure and the mechanism of water oxidation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Multidisciplinary Sciences
Mingjun Xuan, Junbai Li
Summary: Photosystem II (PSII) is a fascinating enzyme involved in photosynthesis that can be coupled with synthetic structures through biomimetic assembly to mimic natural processes. This approach helps gather functions and properties from synthetic materials, inspiring semi-biohybrid research and optimization of artificial light-harvest micro/nanodevices. The combination of PSII with artificial structures offers opportunities for developing biomimetic semi-natural devices and unlocking the secrets of energy conversion in natural photosynthesis.
NATIONAL SCIENCE REVIEW
(2021)
Article
Plant Sciences
Yao-Pin Lin, Yu-Yen Shen, Yen-Bin Shiu, Yee-Yung Charng, Bernhard Grimm
Summary: This study revealed the importance of chlorophyll in energy harvesting and recycling in plant development. The enzymes CLD1 and CHLG are involved in the dephytylation and rephytylation of chlorophyll, and their interaction with other proteins enables the efficient recycling and utilization of chlorophyll.
Article
Plant Sciences
Parisa Rahimzadeh-Karvansara, Guillem Pascual-Aznar, Martina Beckova, Josef Komenda
Summary: Assembly of photosystem II (PSII) requires numerous auxiliary proteins, and this study demonstrates the role of a PSII-associated protein Psb34 in the final conversion of assembly intermediates into functional PSII complexes.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Jiewei Hao, Xueyan Xu, Lina Zhang
Summary: The study revealed significant impacts of seasonal climatic factors on the photochemical performance of terrestrial mosses, with different species developing various mechanisms to cope with adverse abiotic stresses. Some moss species showed good adaptation to habitat temperature and water pattern changes, although they still experienced reduced CO2 assimilation efficiency during drier months, while others were more susceptible to seasonality, especially during periods of lower rainfall and relative humidity. Certain moss species were identified as ideal ecological indicators for photosynthetic acclimation to stressed environments due to their sensitivity to water pattern changes resulting from climate change.
Article
Plant Sciences
Philip J. Jackson, Andrew Hitchcock, Amanda A. Brindley, Mark J. Dickman, C. Neil Hunter
Summary: Quantifying cellular components is crucial for understanding cellular processes and re-engineering cells. In this study, proteins in the cyanobacterium Synechocystis sp. PCC 6803 were quantified using mass spectrometry, revealing important insights into chlorophyll biosynthesis, photosynthesis, and other metabolic processes. The abundance and disparities of different components suggest potential control points for biosynthetic processes and complex assembly.
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Plant Sciences
Yi-Fang Chiu, Han-Yi Fu, Petra Skotnicova, Keng-Min Lin, Josef Komenda, Hsiu-An Chu
Summary: Mutations in the cytochrome (Cyt) b(559) of cyanobacteria lead to PSII deficiency and inability to grow photoautotrophically, but transformants with tandem gene amplification can restore PSII accumulation and achieve photoautotrophic growth.
Article
Plant Sciences
Minna M. Konert, Anna Wysocka, Peter Konik, Roman Sobotka
Summary: High-light-inducible proteins (Hlips) are transmembrane proteins essential for cyanobacteria survival. The Hlip isoforms HliA and HliB bind CP47 and form quenching dimers with Chl and beta-carotene.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Jana Knoppova, Roman Sobotka, Jianfeng Yu, Martina Beckova, Jan Pilny, Joko P. Trinugroho, Ladislav Csefalvay, David Bina, Peter J. Nixon, Josef Komenda
Summary: Analysis of isolated assembly complexes provides new insights into the early stages of photosystem II biogenesis. These complexes contain information about the PSII subunits and proteins and assembly factors associated with its assembly process.
Correction
Multidisciplinary Sciences
Petra Skotnicova, Hristina Staleva-Musto, Valentyna Kuznetsova, David Bina, Minna M. Konert, Shan Lu, Tomas Polivka, Roman Sobotka
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Ivo Sedlacek, Pavla Holochova, Hans-Jurgen Busse, Vendula Koublova, Stanislava Kralova, Pavel Svec, Roman Sobotka, Eva Stankova, Jan Pilny, Ondrej Sedo, Jana Smolikova, Karel Sedlar
Summary: A group of seven bacterial strains producing blue-purple pigmented colonies were isolated from freshwater samples collected in a deglaciated part of Antarctica. Through a comprehensive taxonomic approach, a new Massilia species was identified.
Meeting Abstract
Biochemistry & Molecular Biology
Maria Jose Saucedo Rodriguez, Petr Pecina, Kristyna Cunatova, Marek Vrbacky, Alena Pecinova, Roman Sobotka, Carlo Viscomi, Massimo Zeviani, Tomas Mracek, Josef Houstek
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Multidisciplinary Sciences
Eva Horakova, Laurence Lecordier, Paula Cunha, Roman Sobotka, Piya Changmai, Catharina J. M. Langedijk, Jan Van den Abbeele, Benoit Vanhollebeke, Julius Lukes
Summary: The decreased functionality and expression of HpHbR in Trypanosoma brucei gambiense is a key evolutionary modification that allows this parasite to infect humans. This study reveals that hemoglobin uptake in African trypanosomes is almost exclusively mediated by HpHbR and crucial for their development. Understanding this molecular mechanism is important for our knowledge of how the parasite infects humans and for the development of potential treatments.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Takako Masuda, Keisuke Inomura, Meng Gao, Gabrielle Armin, Eva Kotabova, Gabor Bernat, Evelyn Lawrenz-Kendrick, Martin Lukes, Martina Beckova, Gabor Steinbach, Josef Komenda, Ondrej Prasil
Summary: Crocosphaera and Cyanothece are two unicellular cyanobacteria with nitrogen-fixing abilities that thrive in different environments. Crocosphaera is found mainly in nutrient-depleted open oceans, while Cyanothece is more common in nutrient-rich coastal regions. The differences in their photosynthetic capacities and rates of carbon consumption can sufficiently explain their distinct niches.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Vendula Krynicka, Petra Skotnicova, Philip J. Jackson, Samuel Barnett, Jianfeng Yu, Anna Wysocka, Radek Kana, Mark J. Dickman, Peter J. Nixon, C. Neil Hunter, Josef Komenda
Summary: FtsH proteases are important membrane-embedded proteolytic complexes involved in protein quality control and physiological regulation in bacteria, mitochondria, and chloroplasts. The FtsH homologs in Synechocystis sp. PCC 6803, including FtsH1-FtsH4, play different roles in acclimation to nutrient deficiency and photosystem II quality control. FtsH4 is specifically involved in the biogenesis of photosystem II by regulating the expression and removal of high light-inducible proteins (Hlips).
PLANT COMMUNICATIONS
(2023)
Correction
Plant Sciences
Minna M. Konert, Anna Wysocka, Peter Konik, Roman Sobotka
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Plant Sciences
Jaroslav Nisler, Zuzana Kucerova, Radoslav Koprna, Roman Sobotka, Jana Slivkova, Stephen Rossall, Martina Spundova, Alexandra Husickova, Jan Pilny, Danuse Tarkowska, Ondrej Novak, Maria Skrabisova, Miroslav Strnad
Summary: Increasing crop productivity and mitigating yield losses under stressful conditions are major challenges in agriculture. A recently discovered compound, MTU, delays senescence and enhances the growth of wheat plants. Multiyear field trials demonstrate that treatment with MTU increases average grain yields of wheat and barley by 5-8%. This compound acts independently of cytokinins or other phytohormones and appears to be the only chemical known to affect the stability and activity of PSI.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Ziyu Zhao, Irene Vercellino, Jana Knoppova, Roman Sobotka, James W. Murray, Peter J. Nixon, Leonid A. Sazanov, Josef Komenda
Summary: A suite of accessory factors is required for robust assembly and repair of the oxygen-evolving photosystem two (PSII) complex in oxygenic photosynthesis. The Ycf48 assembly factor, which binds to the D1 reaction center polypeptide, plays a crucial role in PSII assembly. In this study, cryo-electron microscopy is used to determine the structure of a cyanobacterial PSII D1/D2 assembly complex with Ycf48 attached, providing insights into the early stages of PSII assembly and the protective role of Ycf48 in preventing premature binding and damage to the Mn4CaO5 cluster.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Petra Skotnicova, Amit Srivastava, Divya Aggarwal, Jana Talbot, Iva Karlinova, Martin Moos, Jan Mares, Lenka Bucinska, Peter Konik, Petr Simek, Martin Tichy, Roman Sobotka
Summary: The BtpA protein is involved in the biogenesis of photosystems by regulating tetrapyrrole biosynthesis through stabilizing glutamyl-tRNA reductase.
