Article
Engineering, Environmental
Vera Slaveykova, Sanghamitra Majumdar, Nicole Regier, Weiwei Li, Arturo A. Keller
Summary: Metabolomics was used to study the response of green alga to sublethal concentrations of inorganic mercury and monomethylmercury. The alga adjusted its metabolism by increasing levels of various metabolites involved in different pathways after exposure to low concentrations of mercury compounds. Exposure to inorganic mercury resulted in more pronounced metabolic perturbations in comparison to monomethylmercury, while most metabolic disturbances were common between the two treatments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Physical
Huidan Xue, Yibei Dong, Zhihuan Li, Jing Wang, Xiaolong Yuan, Fei He, Zhengke Li, Xiang Gao, Jianxi Liu
Summary: Carbon dots have the ability to enhance photosynthesis in microalgae, promoting cell division and biomass. The red-emitting carbon dots improved the energy transfer and efficiency of photosynthesis, resulting in increased protein and lipid contents. Transcriptome analysis revealed that the carbon dots up-regulated genes associated with cell growth and energy conversion.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Environmental Sciences
Yan Huang, Jinlong Lai, Yang Huang, Xuegang Luo, Xu Yang, Zewei Liu, Yue Duan, Chen Li
Summary: This study investigated the effects of nano-BiOI on freshwater microalgae and its toxicological mechanisms. The results showed that nano-BiOI stimulated algal growth at low concentrations but inhibited growth at higher concentrations. Nano-BiOI induced the generation of intracellular reactive oxygen species and interfered with the mineral nutrient metabolism of the algae. Metabolomics analysis revealed significant alterations in metabolic pathways under high concentrations of nano-BiOI.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sibaji K. Sanyal, Komal Sharma, Diksha Bisht, Sunita Sharma, Kumari Sushmita, Suneel Kateriya, Girdhar K. Pandey
Summary: Ca2+ signaling is crucial for perceiving and transmitting information in cells. The Ca2+-mediated CBL-CIPK module is a major sensor responder network for Ca2+ signaling, and its involvement in plant adaptation from aquatic to terrestrial habitats has been suggested. Experimental analysis showed that CrCBL1 protein interacts with Phototropin and Channelrhodopsin, and its expression is regulated by light. Comparing algal sequences, differences in CBL and CIPK proteins between aquatic and terrestrial plants were identified. Additionally, Klebsormidium CBL and CIPK genes were found to respond to abiotic stress, indicating their early role in terrestrial adaptations. Structure-based prediction and Ca2+-binding assays revealed a typical Ca2+-binding pocket in Klebsormidium's KnCBL1 protein. Overall, this study suggests that stress-responsive proteins facilitate crosstalk between Ca2+ and light signaling pathways during plant adaptation from aquatic to terrestrial habitats.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Environmental Sciences
Wanzhu Li, Meiling Yang, Baoli Wang, Cong-Qiang Liu
Summary: Phytoplankton carbon and nitrogen stoichiometric homeostasis is important in aquatic ecosystems, but the regulatory strategy for its plasticity is unclear. This study investigates the variations in phytoplankton C:N ratios and the regulatory strategy for cellular C-N stoichiometric homeostasis under different C and N availability. The results show that CO2 and nitrate limitations have different effects on phytoplankton C:N ratios, and different gene-regulated intensities are involved in the regulation. This study deepens the understanding of C-N stoichiometric homeostasis in freshwater phytoplankton.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Plant Sciences
Maxence Plouviez, Emilio Fernandez, Arthur Robert Grossman, Emanuel Sanz-Luque, Matthew Sells, David Wheeler, Benoit Guieysse
Summary: The study shows that Chlamydomonas reinhardtii cells are metabolically primed to assimilate and store phosphate, with mutations in VTC genes affecting polyP synthesis and phosphate assimilation. RNA-sequencing data revealed a decrease in VTC gene transcripts after phosphate supply, indicating continued polyP synthesis despite reduced VTC gene expression.
JOURNAL OF PHYCOLOGY
(2021)
Article
Biology
Peter Neofotis, Joshua Temple, Oliver L. Tessmer, Jacob Bibik, Nicole Norris, Eric Pollner, Ben Lucker, Sarathi M. Weraduwage, Alecia Withrow, Barbara Sears, Greg Mogos, Melinda Frame, David Hall, Joseph Weissman, David M. Kramer
Summary: The study shows that in Chlamydomonas reinhardtii, the pyrenoid can be strongly induced by hyperoxia, even in the presence of high levels of CO2 or bicarbonate. This suggests that the pyrenoid may be induced by a common product of photosynthesis specific to low CO2 or hyperoxia conditions. Additionally, evidence is presented for linkages between genetic variations in hyperoxia tolerance, H2O2 signaling, and pyrenoid morphologies.
Article
Plant Sciences
Luo-Yan Zhang, Zhao-Tian Xing, Li-Qian Chen, Xue-Jie Zhang, Shou-Jin Fan
Summary: It is essential to investigate the molecular mechanism of salt stress response in green algae to understand the evolutionary cues of abiotic stress response in plants. In this study, we conducted a comprehensive temporal investigation of transcriptomes in Chlamydomonas reinhardtii GY-D55 cells at different time points. The results revealed a set of common upregulated/downregulated genes and candidate regulatory mechanisms for salt stress responses. Short-term salt stress impaired oxidation-reduction, protein synthesis and modification, and photosynthesis, while the cells employed transcriptional regulation, protein folding, and osmotic stress coping mechanisms. The co-expression module blue was predicted to regulate endoplasmic reticulum (ER) stress at early time points. Additionally, we identified several transcription factors (TFs) with potential roles in algal salt stress response, including Alfin, C2C2, and the MYB family TFs.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Moyan Jia, Jacob Munz, Jenny Lee, Nolan Shelley, Yuan Xiong, Sunjoo Joo, EonSeon Jin, Jae-Hyeok Lee
Summary: Our study identified and characterized the bHLH factor NRI1 in Chlamydomonas, which is required to repress the use of alternative nitrogen sources. NRI1 functions as a critical repressor and coordinates diverse nitrogen starvation-induced responses, providing a crucial first step in understanding how plants and algae cope with fluctuating nitrogen availability.
Article
Biotechnology & Applied Microbiology
Ayat J. S. Al-Azab, Yuichi Aoki, Fean D. Sarian, Yuki Sori, Dwiyantari Widyaningrum, Tomohito Yamasaki, Fantao Kong, Takeshi Ohama
Summary: Exposure to resin nanoparticles composed of isobutyl cyanoacrylate polymers can induce acute cell death in microalgal species. The upregulation of genes encoding antioxidant enzymes and cell wall hydrolytic enzymes plays a key role in inducing cell death in Chlamydomonas reinhardtii.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Isabella Moro, Valerio Matozzo, Emanuela Moschin, Riccardo Trentin, Francesca Dalla Vecchia
Summary: Water pollution by pharmaceutical compounds, with ibuprofen being a commonly found non-steroidal anti-inflammatory drug in European wastewater and aquatic bodies, is a growing environmental issue. This study investigated the effects of ibuprofen on the alga Chlamydomonas reinhardtii, revealing stimulation of growth, morphological and ultrastructural alterations in cells, and no impact on chlorophyll and carotenoids levels.
CHEMISTRY AND ECOLOGY
(2021)
Article
Marine & Freshwater Biology
Giulia Cheloni, Vera Slaveykova
Summary: The study found that under exposure to micropollutants, the green alga Chlamydomonas reinhardtii forms palmelloid colonies as a stress response strategy to face sub-lethal concentrations of micropollutants. Palmelloid formation is a plastic response to micropollutants, helping protect cells from stress.
AQUATIC TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
M. Aguila Ruiz-Sola, Serena Flori, Yizhong Yuan, Gaelle Villain, Emanuel Sanz-Luque, Petra Redekop, Ryutaro Tokutsu, Anika Kuken, Angeliki Tsichla, Georgios Kepesidis, Guillaume Allorent, Marius Arend, Fabrizio Iacono, Giovanni Finazzi, Michael Hippler, Zoran Nikoloski, Jun Minagawa, Arthur R. Grossman, Dimitris Petroutsos
Summary: Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO2 conditions. Depletion of CO2 can drive photoprotection and carbon acquisition even in the absence of light, previously believed to be indispensable for these processes.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Yuval Milrad, Shira Schweitzer, Yael Feldman, Iftach Yacoby
Summary: The metabolism of green algae, particularly the interrelation between NADPH and H-2 accumulation, plays a crucial role in maintaining redox balance under light fluctuations. Energy transfer between H-2 and NADPH is found to be bidirectional and essential for initiating photosynthetic electron flow, as well as dark fermentative metabolism.
Article
Environmental Sciences
Bartosz Plucinski, Beatrycze Nowicka, Andrzej Waloszek, Joanna Rutkowska, Kazimierz Strzalka
Summary: The present study analyzed the protective mechanisms in Chlamydomonas reinhardtii strains adapted and not adapted for growth in the presence of elevated copper concentrations. The copper-adapted strains showed higher levels of antioxidants and photoprotective compounds, as well as increased peroxidase activity and more efficient non-photochemical quenching. These findings suggest that improving antioxidant defense and photoprotection may be crucial factors in the evolution of tolerance to heavy metals.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Lara Bideyan, Wenxin Fan, Karolina Elibieta Kaczor-Urbanowicz, Christina Priest, David Casero, Peter Tontonoz
Summary: Liver X receptors play crucial roles in hepatic metabolism, affecting genes involved in fatty acid metabolism and broad sets of hepatic transcription factors. Loss of LXR binding in liver can lead to increased expression of certain genes and decreased expression of others.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Yang-Tsung Lin, Tomomi Takeuchi, Brian Youk, James Umen, Barbara B. Sears, Christoph Benning
Summary: In the green alga Chlamydomonas reinhardtii, the CHT7 protein plays a critical role in regulating the cell cycle, affecting cell morphology, division, and survival. CHT7 has an opposite regulatory role to mitotic cyclin-dependent kinases, and its absence leads to abnormal expression of cell wall synthesis and remodeling genes. Furthermore, the loss of CHT7 results in the misregulation of minimally characterized kinases, some of which are related to MAP3K and Aurora-like kinases.
G3-GENES GENOMES GENETICS
(2022)
Article
Hematology
Jocelynda Salvador, Gloria E. Hernandez, Feiyang Ma, Cyrus W. Abrahamson, Matteo Pellegrini, Robert Goldman, Karen M. Ridge, M. Luisa Iruela-Arispe
Summary: This study revealed the unexpected contribution of vimentin in driving complete closure of the ductus arteriosus through a mechanism that includes deregulation of the Notch signaling pathway, through single-cell RNA-sequencing and tracking closure of the ductus arteriosus in mice.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marco Morselli, Colin Farrell, Dennis Montoya, Tarik Goren, Ramazan Sabirli, Ibrahim Turkcuer, Ozgur Kurt, Aylin Koseler, Matteo Pellegrini
Summary: The composition of immune cell types changes with age, impacting the response to infectious diseases. By profiling epigenetic marks of immune cells, we can predict pneumonia diagnosis and SARS-CoV-2 viral genome positivity. This DNA methylation-based approach also allows for the prediction of pneumonia severity.
Article
Plant Sciences
Donghee Hoh, Patrick J. Horn, Atsuko Kanazawa, John Froehilch, Jeffrey Cruz, Oliver L. Tessmer, David Hall, Lina Yin, Christoph Benning, David M. Kramer
Summary: The study reveals that chilling sensitivity in cowpea is mainly modulated by specific lipid interactions rather than bulk properties. Accumulation of PG 16:1t may result from upstream effects on photosynthesis, altering redox status and production of reactive oxygen species.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Leila R. Fletcher, Christine Scoffoni, Colin Farrell, Thomas N. Buckley, Matteo Pellegrini, Lawren Sack
Summary: Research has found that there is a decoupling between relative growth rate and adaptation to cold or dry climates in Arabidopsis ecotypes. This decoupling is consistent with the presence of multiple stress resistance and avoidance mechanisms, contributing to the wide geographic range and resilience of the species.
Article
Plant Sciences
Stefanie Boehmer, Christina Marx, Reimund Goss, Matthias Gilbert, Severin Sasso, Thomas Happe, Anja Hemschemeier
Summary: The CrOYE3 enzyme in the green alga Chlamydomonas reinhardtii can convert N-methylmaleimide (NMI) under photosynthesis and plays a significant role in protecting against photooxidative stress.
Article
Biochemistry & Molecular Biology
Rieke Haas, Vera Engelbrecht, Oliver Lampret, Shanika Yadav, Ulf-Peter Apfel, Silke Leimkuehler, Thomas Happe
Summary: The active site of [FeFe]-hydrogenases contains a cubane [4Fe4S]-cluster and a unique diiron cluster with biologically unusual CO and CN ligands. The biogenesis of the diiron site, termed [2FeH], requires the involvement of maturation proteins HydE, HydF, and HydG. However, our study suggests that the proposed binding mechanism and the presence of a [4Fe-4S]-cluster in HydF are not involved in the binding or transfer of [2Fe(P)].
Article
Biochemistry & Molecular Biology
Marco Morselli, Ronan Bennett, Nikko-Ideen Shaidani, Marko Horb, Leonid Peshkin, Matteo Pellegrini
Summary: This study finds that age-associated changes in DNA methylation also exist in amphibians, and whole-genome bisulfite sequencing (WGBS) profiles from skin samples of nine frogs were used to characterize the gene- and chromosome-scale features of age-associated DNA methylation changes. The results suggest that the mechanism of DNA methylation changes in amphibians is similar to that in mammals. Additionally, a targeted DNA methylation analysis (TBSseq) assay was developed for future studies with larger cohorts of individuals. The findings of this study will contribute to understanding the relationship between DNA methylation and other hallmarks of aging using the unique resources available for Xenopus.
Correction
Endocrinology & Metabolism
David A. Salisbury, David Casero, Zhengyi Zhang, Dan Wang, Jason Kim, Xiaohui Wu, Laurent Vergnes, Aashiq H. Mirza, Paola Leon-Mimila, Kevin J. Williams, Adriana Huertas-Vazquez, Samie R. Jaffrey, Karen Reue, Jianjun Chen, Tamer Sallam
Article
Cell Biology
Giulia Protti, Liudmilla Rubbi, Tarik Goren, Ramazan Sabirli, Serkan Civlan, Ozgur Kurt, Ibrahim Turkcuer, Aylin Koseler, Matteo Pellegrini
Summary: This study aimed to investigate the association between the methylome of buccal epithelial cells and demographic and health-related variables. Multiple factors, including age, sex, blood immune composition, and hemoglobin levels, were found to accurately model DNA methylation variation. The identified genomic loci associated with these factors were enriched in relevant transcription factors, drugs, and diseases. This study contributes to a better understanding of human methylome plasticity and highlights the potential clinical implications of considering these physiological traits in medical epigenomic investigations.
PHYSIOLOGICAL GENOMICS
(2023)
Article
Plant Sciences
Yang Xu, Shrikaar Kambhampati, Stewart A. Morley, Ron Cook, John Froehlich, Doug K. Allen, Christoph Benning
Summary: The study found that ACP4 and RBL10 can affect chloroplast lipid biosynthesis by modulating substrate precursor pools, thus participating in chloroplast lipid assembly and monogalactosyldiacylglycerol biosynthesis. This study shows that Arabidopsis ACP4 and RBL10 independently affect chloroplast membrane lipid biosynthesis.
Correction
Endocrinology & Metabolism
David A. Salisbury, David Casero, Zhengyi Zhang, Dan Wang, Jason Kim, Xiaohui Wu, Laurent Vergnes, Aashiq H. Mirza, Paola Leon-Mimila, Kevin J. Williams, Adriana Huertas-Vazquez, Samie R. Jaffrey, Karen Reue, Jianjun Chen, Tamer Sallam
Article
Chemistry, Multidisciplinary
Melanie Heghmanns, Andreas Rutz, Yury Kutin, Vera Engelbrecht, Martin Winkler, Thomas Happe, Muege Kasanmascheff
Summary: In this study, the active center of CbA5H, the H-cluster, was characterized using multifrequency continuous wave and pulsed electron paramagnetic resonance spectroscopy. It was found that under oxidizing conditions, an additional radical species dominates the spectra. The generation of this radical signal depends on the presence of an intact H-cluster and a complete proton transfer pathway.