Article
Chemistry, Analytical
Alexander S. Goryashchenko, Alexey A. Pakhomov, Anastasia Ryabova, Igor D. Romanishkin, Eugene G. Maksimov, Alexander N. Orsa, Oxana Serova, Andrey A. Mozhaev, Margarita A. Maksimova, Vladimir Martynov, Alexander G. Petrenko, Igor E. Deyev
Summary: A genetically encoded fluorescent sensor, SypHerExtra, was developed to measure extracellular media pH in a mildly alkaline range. This sensor can be used in live eukaryotic cells, along with another sensor, SypHer3s, which measures intracellular pH, for quantitative measurements using the FLIM method.
Article
Plant Sciences
Alex Rajewski, Dinusha C. Maheepala, Jessica Le, Amy Litt
Summary: By comparing the fruit development and gene expression patterns of multiple species, we found that the expression of core fruit development genes is somewhat conserved. These genes play important roles in cell division, cell wall synthesis, and developmental patterning. In addition, we identified accessory genes with conserved expression patterns within different fruit types, highlighting the significant differences between these phenotypic classes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Geosciences, Multidisciplinary
Nikolay N. Slyunyaev, Alexander V. Frank-Kamenetsky, Nikolay V. Ilin, Fedor G. Sarafanov, Maria V. Shatalina, Evgeny A. Mareev, Colin G. Price
Summary: Electric field measurements at Vostok station in Antarctica show that the El Nino-Southern Oscillation (ENSO) has a significant impact on the global electric circuit. Analysis from 2006 to 2016 reveals that the diurnal variation of the surface electric field differs significantly between El Nino and La Nina years, attributed to changes in the global distribution of electrified clouds during ENSO events.
GEOPHYSICAL RESEARCH LETTERS
(2021)
Article
Oncology
Bareun Kim, Anthony T. Lopez, Indhujah Thevarajan, Maria F. Osuna, Monica Mallavarapu, Boning Gao, Jihan K. Osborne
Summary: Cell motility is important in various physiological processes, including tumor metastasis and wound healing. Research on human cells has shown that normal cells migrate faster than cancer cells, suggesting potential targets for future therapies.
Article
Chemistry, Analytical
Jorge Espina-Casado, Tania Fontanil, Alfonso Fernandez-Gonzalez, Santiago Cal, Alvaro J. Obaya, Marta Elena Diaz-Garcia, Rosana Badia-Laino
Summary: Carbon dots were synthesized from glutathione and surface-modified with beta-Cyclodextrin and FP, showing potential applications as drug carriers. The fluorescence changes allowed distinction of cancer cells with no alterations in cell morphology, enabling bioimaging and cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Inorganic & Nuclear
Andrew J. Counsell, Mingfeng Yu, Mengying Shi, Angus T. Jones, James M. Batten, Peter Turner, Matthew H. Todd, Peter J. Rutledge
Summary: The coordination chemistry of N-functionalised cyclam ligands with pendant alkynes was investigated, revealing unexpected coordination modes and the formation of multiple unique complexes within a single recrystallisation. Surprisingly, weak intramolecular copper-alkyne coordination and intramolecular copper-mediated hydroalkoxylation reaction were observed in some of the copper complexes. Multiple functionalisation of the ligands was demonstrated via a 'tetra-click' reaction with benzyl azide, and the resulting tetra-triazole ligand's copper-binding behavior was characterized spectroscopically.
DALTON TRANSACTIONS
(2021)
Article
Plant Sciences
Huiyun Yu, Leiyun Yang, Zhan Li, Feng Sun, Bo Li, Shengsong Guo, Yong-Fei Wang, Tong Zhou, Jian Hua
Summary: In situ deletion series generated by CRISPR/Cas9 genome editing identified cis-regulatory elements and potential transcription factors for the expression of the intracellular immune receptor NLR gene SNC1, and revealed the critical role of SNC1 in NLR gene co-expression.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Huiyun Yu, Leiyun Yang, Zhan Li, Feng Sun, Bo Li, Shengsong Guo, Yong-Fei Wang, Tong Zhou, Jian Hua
Summary: The transcriptional and post-transcriptional regulation of intracellular immune receptor nucleotide-binding leucine-rich repeats (NLRs) is complex and involves multiple cis-acting elements. The NLR gene SNC1 is a critical regulator of gene expression in the same gene cluster, and its expression is under complex local regulation.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Environmental Sciences
Jun Li, Xuexi Tang, Kehou Pan, Baohua Zhu, Yun Li, Ziqi Wang, Yan Zhao
Summary: By studying the metabolic acclimation strategies of different Chlorella strains to high CO2, we identified differences in energy metabolism, cytoplasmic pH regulation, and organic carbon flow. Furthermore, our study provided new traits for breeding microalgae for CO2 biosequestration.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Medicine, Research & Experimental
Qi Li, Xiaoling Qin, Xiaotong Kou, Jingyu Li, Zhongsha Li, Chang Chen
Summary: The study showed that anagliptin can reduce tumor growth and induce apoptosis of cancer cells by regulating lactate excretion and intracellular pH gradient. Anagliptin reverses the abnormal pH gradient by suppressing lactate excretion, reducing mitochondrial membrane potential, and inducing apoptosis. These findings suggest that anagliptin may be a potential treatment for cancer.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Oncology
Gilberto Gastelum, Jeffry Kraut, Mysore Veena, Alisher Baibussinov, Christopher Lamb, Kylee Lyons, Eric Y. Chang, Patrick Frost
Summary: Hypoxia-induced apoptosis of MM cells is correlated with acidic intracellular pH. Targeting intracellular pH may be more effective at treating cancers within a hypoxic TME.
FRONTIERS IN ONCOLOGY
(2023)
Article
Plant Sciences
Rainer Waadt, Joerg Kudla, Hannes Kollist
Summary: Understanding plant physiology at the organismic scale involves analyzing biological processes down to the cellular and molecular levels. Fluorescent protein (FP) technology allows for direct monitoring of various cellular dynamics in plants, and multiplexing techniques enable simultaneous recording of multiple biological processes.
Article
Microbiology
Shannon L. Kordus, Heather K. Kroh, Ruben Cano Rodriguez, Rebecca A. Shrem, F. Christopher Peritore-Galve, John A. Shupe, Brian E. Wadzinski, D. Borden Lacy, Benjamin W. Spiller
Summary: Clostridioides difficile is a leading cause of antibiotic-associated diarrhea and nosocomial infection in the United States. This article introduces the development of nanobody tools and detection methods for studying the toxins TcdA and TcdB produced by C.difficile. These tools are expected to help researchers understand the production and dynamics of the toxins, as well as the impact of various experimental interventions on toxin production.
Review
Chemistry, Multidisciplinary
Uroob Haris, Husain N. Kagalwala, Yujin Lisa Kim, Alexander R. Lippert
Summary: The article discusses the use of chemiluminescence for imaging and monitoring reactive analytes in biological samples, showcasing the successful development of bright chemiluminescent probes for cell monitoring and in vivo imaging. The tools have been applied to study complex signaling pathways and enzyme activity, demonstrating the potential for commercialization and clinical translation.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Cell Biology
Shiao-Pieng Lee, Shih-Chi Chao, Mei-Fang Chou, Shu-Fu Huang, Niann-Tzyy Dai, Gwo-Jang Wu, Chien-Sung Tsai, Shih-Hurng Loh, Yi-Ting Tsai
Summary: The study demonstrates the importance of passive pH(i)buffering power in cell differentiation and highlights the critical role of NHE1 activity in maintaining pluripotency in hiPSCs.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Agronomy
Javier Matias, Maria Jose Rodriguez, Veronica Cruz, Patricia Calvo, Maria Reguera
Summary: The study evaluated the agronomic performance, seed composition, and nutrient uptake of five quinoa varieties under Mediterranean field conditions in Southwest Europe over two consecutive years. High temperatures in 2017 led to significantly lower yields compared to 2018, highlighting the impact of heat stress on quinoa cultivation.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2021)
Article
Plant Sciences
Rosario Castro-Rodriguez, Viviana Escudero, Maria Reguera, Patricia Gil-Diez, Julia Quintana, Rosa Isabel Prieto, Rakesh K. Kumar, Ella Brear, Louis Grillet, Jiangqi Wen, Kirankumar S. Mysore, Elsbeth L. Walker, Penelope M. C. Smith, Juan Imperial, Manuel Gonzalez-Guerrero
Summary: Yellow Stripe-Like (YSL) proteins, including MtSYL7, play an essential role in transition metal homeostasis in nodules. MtSYL7 is involved in transporting short peptides and contributes to maintaining metal homeostasis in root nodules, impacting nitrogen fixation and plant growth. Mutants with decreased MtYSL7 expression accumulate more copper and iron in nodules due to increased expression of iron uptake and delivery genes in roots.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Sara Granado-Rodriguez, Nieves Aparicio, Javier Matias, Luis Felipe Perez-Romero, Isaac Maestro, Irene Graces, Justo Javier Pedroche, Claudia Monika Haros, Nieves Fernandez-Garcia, Joaquin Navarro del Hierro, Diana Martin, Luis Bolanos, Maria Reguera
Summary: The study on Chenopodium quinoa Willd (quinoa) has shown that the nutritional properties of quinoa seeds are significantly influenced by the year of cultivation and different genotypes. The research found variations in nutritional parameters among different quinoa cultivars and identified 2018 as a year with lower yields, germination rates, but higher seed weights and seed protein contents. This work contributes to increased understanding of the impact of environment and genotype on the nutritional properties of quinoa seeds, particularly in regions with similar climatic conditions to Southern Europe.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Javier Matias, Veronica Cruz, Maria Reguera
Summary: This study analyzed the straw yield, composition, and relative feed value of five quinoa varieties under field conditions in Southwestern Europe. It was found that high temperatures led to decreased straw yield, increased feed value, and compositional changes. Varieties differed in terms of straw yield and mineral contents.
Review
Biochemistry & Molecular Biology
Houssein Monder, Morgan Maillard, Isabelle Cherel, Sabine Dagmar Zimmermann, Nadine Paris, Teresa Cuellar, Isabelle Gaillard
Summary: Grapes, economically important for their high fruit value and significance in winemaking, are currently facing decreased quality and production due to abiotic constraints from climate change. Potassium plays a crucial role in grapevine growth, composition, and wine quality, impacting berry loading during ripening and defenses against stress. Understanding potassium's functions can aid in selecting crops better suited for adverse environmental conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Sara Granado-Rodriguez, Susana Vilarino-Rodriguez, Isaac Maestro-Gaitan, Javier Matias, Maria Jose Rodriguez, Patricia Calvo, Veronica Cruz, Luis Bolanos, Maria Reguera
Summary: Analyzing the relationship between the nutritional properties of seeds and genetic background is crucial for understanding and improving the nutritional quality of crops. This study focused on analyzing seed nutritional parameters in 15 quinoa cultivars grown in a specific environmental context, identifying important agronomical and nutritional differences among the cultivars. The findings emphasize the importance of considering genotypic variation in quinoa selection for improved varieties with optimal nutritional characteristics.
Article
Plant Sciences
Hortense Moreau, Isabelle Gaillard, Nadine Paris
Summary: Using genetically encoded tandems of fluorescent proteins, this study demonstrates the complex environment and pH heterogeneity within pavement cells. The newly designed acid-sensing tandems cover the pH range of 3-8 and show immediate responses to pH changes, providing insights into physiological conditions related to cell elongation and H+-ATPase activity.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Nutrition & Dietetics
Javier Matias, Maria Jose Rodriguez, Sara Granado-Rodriguez, Veronica Cruz, Patricia Calvo, Maria Reguera
Summary: This study found that elevated temperatures can reduce the oil content and quality of quinoa seeds. Fertilization can optimize oil quality by controlling mineral nutrient levels.
FRONTIERS IN NUTRITION
(2022)
Article
Agronomy
Narciso M. Quijada, Isidro Abreu, Maria Reguera, Ildefonso Bonilla, Luis Bolanos
Summary: Boron (B) was found to have significant effects on the growth and survival of several strains of Ensifer meliloti, enhancing their tolerance to salt, cell surface stressors, and oxidative stress. Boron deficiency resulted in reduced exopolysaccharide (EPS) synthesis and altered gene expression related to EPS production.
Article
Nutrition & Dietetics
Sara Granado-Rodriguez, Isaac Maestro-Gaitan, Javier Matias, Maria Jose Rodriguez, Patricia Calvo, Luis Eduardo Hernandez, Luis Bolanos, Maria Reguera
Summary: Seed storage temperature and moisture content play crucial roles in maintaining or improving the nutritional quality of quinoa seeds. The use of silica gel has limited impact on seed quality when the moisture content is between 5% and 12%. Lower storage temperatures (4 degrees C) can help preserve or enhance the nutritional quality of quinoa seeds, while higher temperatures (37 degrees C) can negatively affect seed quality.
FRONTIERS IN NUTRITION
(2022)
Editorial Material
Plant Sciences
Maria Reguera, Juan Jose Camacho-Cristobal
Review
Plant Sciences
Luis Bolanos, Isidro Abreu, Ildefonso Bonilla, Juan J. Camacho-Cristobal, Maria Reguera
Summary: On the eve of the 100th anniversary of Dr. Warington's discovery of boron (B) as a nutrient essential for higher plants, boronists have struggled to demonstrate a role beyond its structural function in cell walls dimerizing pectin molecules of rhamnogalacturonan II (RGII). In this regard, B deficiency has been associated with a plethora of symptoms in plants that include macroscopic symptoms like growth arrest and cell death and biochemical or molecular symptoms that include changes in cell wall pore size, apoplast acidification, or a steep ROS production that leads to an oxidative burst. Aiming to shed light on B functions in plant biology, we proposed here a unifying model integrating the current knowledge about B function(s) in plants to explain why B deficiency can cause such remarkable effects on plant growth and development, impacting crop productivity. In addition, based on recent experimental evidence that suggests the existence of different B ligands other than RGII in plant cells, namely glycolipids, and glycoproteins, we proposed an experimental pipeline to identify putative missing ligands and to determine how they would integrate into the above-mentioned model.
Article
Biotechnology & Applied Microbiology
Isaac Maestro-Gaitan, Sara Granado-Rodriguez, Miguel Redondo-Nieto, Antonio Battaglia, Laura Poza-Viejo, Javier Matias, Luis Bolanos, Maria Reguera
Summary: This study examines the impact of drought on quinoa rhizosphere bacterial communities and finds that genotype, water environment, and type of inoculant are determining factors in shaping these communities, affecting plant performance.
MICROBIAL BIOTECHNOLOGY
(2023)
Review
Plant Sciences
Hortense Moreau, Sabine D. Zimmermann, Isabelle Gaillard, Nadine Paris
Summary: The pH parameter of soil is crucial for plant nutrition and growth by affecting mineral availability. Technological advancements now allow for real-time monitoring of pH at subcellular levels, providing insights into its impact on plant development.
Article
Plant Sciences
Aleksandr Gavrin, Patrick C. Loughlin, Ella Brear, Oliver W. Griffith, Frank Bedon, Marianne Suter Grotemeyer, Viviana Escudero, Maria Reguera, Yihan Qu, Siti N. Mohd-Noor, Chi Chen, Marina Borges Osorio, Doris Rentsch, Manuel Gonzalez-Guerrero, David A. Day, Penelope Mary Collina Smith
Summary: The study identified GmYSL7 in soybean nodules as a transporter of oligopeptides, unlike most YSL family members that transport metals complexed with phytosiderophores. Silencing GmYSL7 reduces nitrogenase activity and blocks infected cell development, indicating the importance of its substrate for proper nodule development. RNAseq analysis of silenced nodules showed a defense response against rhizobia as well as alterations in genes related to amino acid export and metal homeostasis. These changes suggest that the peptides transported by GmYSL7 play a role in monitoring bacteroid function and regulating nodule metabolism and transport processes. Further research is needed to clarify the specific physiological substrate of GmYSL7.