Article
Plant Sciences
Bing Li, Xueli Zhang, Zhiquan Liu, Lulin Wang, Liping Song, Xiaomei Liang, Shengwei Dou, Jinxing Tu, Jinxiong Shen, Bin Yi, Jing Wen, Tingdong Fu, Cheng Dai, Changbin Gao, Aihua Wang, Chaozhi Ma
Summary: Most flowering plants have developed a self-incompatibility system to maintain genetic diversity, with Brassica species having sporophytic self-incompatibility. The discovery of a new self-compatible chromosome BrS-325 in a pak choi line '325' shows potential practical breeding applications in B. rapa. The study also reveals high sequence polymorphism and amino acid variations in the S locus, which may affect the interaction between ligands and receptors in SI systems.
Article
Multidisciplinary Sciences
Ji-Ung Jung, Melanie H. Cobb
Summary: The protein kinase WNK1 influences the trafficking and actin polymerization of membrane proteins. This study discovered a connection between WNK1 and TRIM27, which regulates endosomal actin polymerization. Depletion of WNK1 disrupted WASH ubiquitination and endosomal actin polymerization, affecting endosomal trafficking. Depletion of WNK1 or TRIM27 increased the degradation of EGFR in cancer cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Masaya Yamamoto, Hiroyasu Kitashiba, Takeshi Nishio
Summary: This study successfully generated self-incompatibility in Arabidopsis thaliana by introducing Brassica rapa's SRK and SCR genes. Mutations in the amino acid residues were found to affect the response of self-incompatibility. The method developed in this study is of great significance for studying the self-recognition mechanisms in Brassicaceae.
Article
Genetics & Heredity
Qinqin Xie, Hecui Zhang, Dengke Hu, Qianying Liu, Tonghong Zuo, Yizhong Zhang, Yimei Liu, Siru Zhou, Liquan Zhu
Summary: This study identified 16 members of the BoGAPDH family in Brassica oleracea, most of which were down-regulated during self-pollination with the highest expression in stigmas and sepals. The gene BoGAPC may be involved in self-incompatibility process and interact with SRK kinase domain for stress resistance.
Review
Biochemistry & Molecular Biology
Maria J. Iraburu, Tommy Garner, Cristina Montiel-Duarte
Summary: The endocytosis of ligand-bound receptors and their recycling to the plasma membrane are important for cell functions. The insulin receptor can be endocytosed by clathrin-dependent and independent mechanisms, and the sorting of the receptor at the early endosome determines the intensity and duration of insulin effects. Various proteins, including the Arf and Rab families of small GTPases, regulate the endocytic and endosomal pathways. This article argues for a specific role of the slow recycling route in INSR endocytosis and provides a review of the molecular mechanisms involved.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Hongjian He, Jiaqi Guo, Jiashu Xu, Jiaqing Wang, Shuang Liu, Bing Xu
Summary: This study demonstrates how nanoscale phosphopeptide assemblies cluster ALP to enable cellular uptake through caveolae-mediated endocytosis and endosomal escape. Dephosphorylation of phosphopeptides leads to the formation of peptidic nanofibers for endosomal escape. Inhibiting TNAP or disrupting lipid rafts abolishes the endocytosis process, while decreasing the transformation to nanofibers prevents endosomal escape.
Article
Plant Sciences
Fang Yue, Fajing Zheng, Qinfei Li, Jiaqin Mei, Chunlei Shu, Wei Qian
Summary: Improving Brassica napus through introgression of genome components from B. oleracea and B. rapa is an effective breeding strategy. While interspecific hybridization between B. napus and B. rapa is successful, hybridization between B. napus and B. oleracea faces challenges due to high embryo abortion rates. Transcriptome sequencing of B. napus siliques pollinated with B. oleracea and B. rapa revealed complex transcriptional changes and dysregulated plant hormone metabolism in the incompatible hybridization, possibly contributing to the accumulation of reactive oxygen species (ROS) and inhibition of cell morphogenesis.
Article
Plant Sciences
Zhiquan Liu, Bing Li, Yong Yang, Changbin Gao, Bin Yi, Jing Wen, Jinxiong Shen, Jinxing Tu, Tingdong Fu, Cheng Dai, Chaozhi Ma
Summary: This study detected the distribution of S haplotypes in B. napus and determined the function of a common S haplotype BnS-6. The expression of SRK was normal in different S haplotypes, while the low expression of BnSCR-6 may lead to the inactivation of BnS-6 in the SI response of B. napus.
Review
Horticulture
Fenghua Wang, Yufei Li, Guangyuan Li, Shuangchen Chen
Summary: Brassica vegetables play a vital role in human nutrition, and self-incompatibility is a common characteristic in this plant family. Breeding through self-incompatibility lines is an important method to harness the heterosis of Brassica vegetables. The self-incompatibility inheritance in Brassica species is controlled by three linkage genes on the S-locus, namely SRK, SCR/SP11, and SLG. This review also highlights the regulatory effects of other functional molecules on self-incompatibility.
Article
Multidisciplinary Sciences
Paula P. Coelho, Geoffrey G. Hesketh, Annika Pedersen, Elena Kuzmin, Anne-Marie N. Fortier, Emily S. Bell, Colin D. H. Ratcliffe, Anne-Claude Gingras, Morag Park
Summary: This study uncovers an LC3C-Endocytic-Associated-Pathway (LEAP) that selectively recruits plasma membrane cargo to autophagosomes, providing new understanding of the selective coupling of plasma membrane signaling with autophagic degradation.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Joy Kar, Sumanta Kar, Arnab Gupta, Siddhartha S. Jana
Summary: Endocytic vesicular trafficking requires the merging of two lipid bilayers, and the involvement of nonmuscle myosin IIs (NM IIs) in fusion and fission processes has been explored. Knocking down NM IIs or inhibiting their activities leads to the formation of ring-like assemblies of early endosomes (raEE). The assembly and disassembly dynamics of NM II play a role in regulating fission and maintaining the size of early endosomes.
Article
Horticulture
Ayda Boubakri, Lamia Krichen, Mohamed-Amine Batnini, Neila Trifi-Farah, Guillaume Roch, Jean-Marc Audergon, Hedia Bourguiba
Summary: The study identified diverse S-alleles within the Tunisian apricot germplasm, allocating the accessions into 14 cross-incompatibility groups, with newly identified self-compatible accessions. This reflects the domestication and diffusion history of apricot species. Understanding the incompatibility relationships among apricot accessions is crucial for future breeding programs and orchard design.
SCIENTIA HORTICULTURAE
(2021)
Article
Chemistry, Applied
Katharina Friedrich, Nicole S. Wermter, Lars Andernach, Katja Witzel, Franziska S. Hanschen
Summary: This study investigated the accumulation and formation of sulfur-containing compounds in Brassica vegetables. The results showed that SMCSO was the main sulfur compound, and it degraded to other compounds upon heating. The SMCSO content was similar in white and red cabbages. Proteome profiling revealed a correlation between the recovery of sulfur compounds and the abundance of two cystine lyase isoforms.
Article
Plant Sciences
Wendi Chen, Bin Zhang, Wenjing Ren, Li Chen, Zhiyuan Fang, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Yangyong Zhang
Summary: In this study, the self-compatibility of 58 winter cabbage inbred lines was identified using open-flower self-pollination and molecular techniques. A new S-haplotype-specific marker was developed and 9 distinct S-haplotypes were found in the cabbage inbred lines. A Tri-Primer-PCR amplification method was established for rapid selection of plants with specific S-haplotypes. The study also overcame parental cross-incompatibility by selecting an inbred line with a specific S-haplotype.
Editorial Material
Biochemistry & Molecular Biology
Muhammad Jamshed, Neil M. N. Hickerson, Subramanian Sankaranarayanan, Marcus A. Samuel
Summary: Exciting new research reveals the interaction between stigmatic receptors for recognizing self-incompatible pollen and the FERONIA pathway, which regulates the production of stigmatic reactive oxygen species to establish barriers against self-, intra- and interspecific pollen.
Article
Cell Biology
Regina Gratz, Prabha Manishankar, Rumen Ivanov, Philipp Koester, Inga Mohr, Ksenia Trofimov, Leonie Steinhorst, Johannes Meiser, Hans-Joerg Mai, Maria Drerup, Sibylle Arendt, Michael Holtkamp, Uwe Karst, Joerg Kudla, Petra Bauer, Tzvetina Brumbarova
DEVELOPMENTAL CELL
(2019)
Article
Plant Sciences
Imran Khan, Regina Gratz, Polina Denezhkin, Stephan N. Schott-Verdugo, Kalina Angrand, Lara Genders, Rubek Merina Basgaran, Claudia Fink-Straube, Tzvetina Brumbarova, Holger Gohlke, Petra Bauer, Rumen Ivanov
Article
Plant Sciences
Antonella Succurro, Mara Schuler-Bermann, Rumen Ivanov, Richard Jacoby, Stanislav Kopriva, Timothy O. Jobe
Article
Plant Sciences
Regina Gratz, Tzvetina Brumbarova, Rumen Ivanov, Ksenia Trofimov, Laura Tuennermann, Rocio Ochoa-Fernandez, Tim Blomeier, Johannes Meiser, Stefanie Weidtkamp-Peters, Matias D. Zurbriggen, Petra Bauer
Article
Agronomy
Ramakrishnan Madhavan Nair, Bindumadhava HanumanthaRao, Anilkumar Vemula, Abhishek Rathore, Tzvetina Brumbarova, Rumen Ivanov
Article
Plant Sciences
Frederique Rozier, Lucie Riglet, Chie Kodera, Vincent Bayle, Eleonore Durand, Jonathan Schnabel, Thierry Gaude, Isabelle Fobis-Loisy
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Plant Sciences
Rumen Ivanov, David G. Robinson
Review
Cell Biology
Rumen Ivanov, Gregory Vert
Summary: The removal of transmembrane proteins from the plasma membrane via endocytosis is an important strategy in regulating receptor signaling and molecule transport. Studying plant endocytosis, particularly focusing on metal transporters like IRT1, has revealed new factors and mechanisms involved in endocytic trafficking, contributing to a better understanding of this process in maintaining plant metal homeostasis.
BIOLOGY OF THE CELL
(2021)
Article
Biology
Lucie Riglet, Frederique Rozier, Chie Kodera, Simone Bovio, Julien Sechet, Isabelle Fobis-Loisy, Thierry Gaude
Correction
Plant Sciences
Rumen Ivanov, David G. Robinson
Review
Plant Sciences
Regina Gratz, Claudia von der Mark, Rumen Ivanov, Tzvetina Brumbarova
Summary: The fluctuation of soil iron availability poses a significant challenge for plants, with calcium and reactive oxygen species coordinating signaling events related to iron acquisition. Calcium affects iron import through IRT1 and triggers a signaling pathway influencing the activity of the key iron-acquisition transcription factor FIT, while reactive oxygen species both inhibit FIT function and rely on FIT through the function of catalase CAT2 under prolonged iron deficiency.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucie Riglet, Frederique Rozier, Isabelle Fobis-Loisy, Thierry Gaude
Summary: Following pollen deposition on the stigma, a pollen tube germinates to transport male gametes to the ovule for fertilization. The orientation of KATANIN and CMT plays a crucial role in guiding the pollen tube towards the ovule.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Plant Sciences
Claudia von der Mark, Rumen Ivanov, Monique Eutebach, Veronica G. Maurino, Petra Bauer, Tzvetina Brumbarova
Summary: Reactive oxygen species play a crucial role in regulating plant responses to environmental stress, with hydrogen peroxide levels increasing under prolonged iron deficiency to inhibit Fe acquisition through the regulation of FIT. CAT2 and FIT are involved in a regulatory loop between H2O2 and prolonged Fe deficiency, influencing downstream regulators in the Fe homeostasis transcription cascade.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Ksenia Trofimov, Rumen Ivanov, Monique Eutebach, Buesra Acaroglu, Inga Mohr, Petra Bauer, Tzvetina Brumbarova
Article
Multidisciplinary Sciences
Tzvetina Brumbarova, Rumen Ivanov