Article
Biochemistry & Molecular Biology
Patricia Ballester, Maria A. Martinez-Godoy, Miguel Ezquerro, Marisa Navarrete-Gomez, Marina Trigueros, Manuel Rodriguez-Concepcion, Cristina Ferrandiz
Summary: The stigma, an essential tissue for pollination in angiosperms, involves multiple transcription factors in Arabidopsis thaliana. The NGATHA (NGA) and HECATE (HEC) transcription factors interact physically in the apical gynoecium and are crucial for stigma differentiation through upregulating other key genes. This study suggests a nonhierarchical regulatory scenario in which the combinatorial action of different transcription factors provides precise temporal and spatial specificity for developmental outputs.
Review
Plant Sciences
Juan Ramos-Pulido, Stefan de Folter
Summary: The success of angiosperms, the most successful group of land plants, is mainly attributed to the gynoecium, the innermost whorl of the flower. In Arabidopsis, the gynoecium is formed by two fused carpels, and a quasi-meristem called the carpel margin meristem plays a crucial role in its development. The study of gynoecium development, which is important for the production of seeds and fruits, has been a significant task for the scientific community. This review presents recent advances in Arabidopsis gynoecium patterning and highlights unanswered questions.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cheng Zhang, Kailu Zhang, Zihan Chai, Yanfeng Song, Xianrong Wang, Yifan Duan, Min Zhang
Summary: This study investigated miRNA in Osmanthus fragrans and found that families such as miRNA160, miRNA167, miRNA393, and miRNA396 may influence sex differentiation in this androdioecious plant. The findings lay a foundation for further exploration of the molecular mechanisms of androdioecy in O. fragrans from a miRNA sequencing perspective.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiaoyu Liu, Mengjuan Tong, Aiwei Zhang, Mei Liu, Bingchun Zhao, Zhaojiao Liu, Zhouyue Li, Xu Zhu, Yi Guo, Rui Li
Summary: This study investigated the function of SEC31A and compared it with SEC31B in Arabidopsis, revealing differences in co-localization rates in pollen and pollen tubes. SEC31A and SEC31B play complementary roles in pollen development, with an essential role in gametogenesis highlighted by the study.
Article
Plant Sciences
Alex Rajewski, Dinusha C. Maheepala, Jessica Le, Amy Litt
Summary: By comparing the fruit development and gene expression patterns of multiple species, we found that the expression of core fruit development genes is somewhat conserved. These genes play important roles in cell division, cell wall synthesis, and developmental patterning. In addition, we identified accessory genes with conserved expression patterns within different fruit types, highlighting the significant differences between these phenotypic classes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Sophia V. Yudina, Alexander Kocyan, Ba Vuong Truong, Nikolay A. Vislobokov, Dmitry F. Lyskov, Maxim S. Nuraliev, Margarita V. Remizowa
Summary: This study provides a comprehensive investigation of flowers and inflorescences of the Burmannia genus, describing the diversity of inflorescence architecture and early floral development. Additionally, the study explores the floral vasculature and stamen-style interactions in Burmannia.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Humberto Herrera-Ubaldo, Sergio E. Campos, Pablo Lopez-Gomez, Valentin Luna-Garcia, Victor M. Zuniga-Mayo, Gerardo E. Armas-Caballero, Karla L. Gonzalez-Aguilera, Alexander DeLuna, Nayelli Marsch-Martinez, Carlos Espinosa-Soto, Stefan de Folter
Summary: In this study, the formation of the gynoecium, the female reproductive part of the flower, was investigated using a systems biology approach. The study identified numerous interactions between transcription factors and protein-protein interactions, providing valuable insights for the plant community.
Article
Plant Sciences
Zhihua Cheng, Xiaofei Song, Xiaofeng Liu, Shuangshuang Yan, Weiyuan Song, Zhongyi Wang, Lijie Han, Jianyu Zhao, Liying Yan, Zhaoyang Zhou, Xiaolan Zhang
Summary: This study revealed the functions of the genes CsSPT and CsALC in regulating fruit cavity formation and female fertility determination in cucumber. These two genes mediate the expression of genes involved in transmitting tract development, auxin-mediated signaling, and cell wall organization, and redundantly define stigma morphology and the pollen tube extension path. This ensures female fertility and carpel fusion in cucumber.
Review
Developmental Biology
Humberto Herrera-Ubaldo, Stefan de Folter
Summary: The article highlights recent discoveries on the development of gynoecium and fruit in Arabidopsis, including the factors, interactions, and mechanisms involved. It also presents the currently known gene regulatory networks from gynoecium initiation to fruit maturation.
Article
Plant Sciences
Michela Osnato, Elia Lacchini, Alessandro Pilatone, Ludovico Dreni, Andrea Grioni, Matteo Chiara, David Horner, Soraya Pelaz, Martin M. Kater
Summary: Floral homeotic genes in angiosperms, such as OsMADS13 in rice, play a crucial role in determining ovule identity. The interaction between OsMADS13 and other genes, like those in the SEP family, may control a common set of target genes involved in ovule development. This study sheds light on the regulatory pathways in rice gynoecium development and provides insights into the role of OsMADS13 as a repressor of the carpel pathway during ovule development.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Biochemistry & Molecular Biology
Naghmeh Abiri, Andrey Sinjushin, Dilek Tekdal, Selim Cetiner
Summary: Understanding the regulatory genes affecting carpel number can improve agricultural yield, as multicarpellate plants may provide significantly higher yields than plants with fewer carpels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Valentin Verges, Christelle Dutilleul, Beatrice Godin, Boris Collet, Alain Lecureuil, Loic Rajjou, Cyrille Guimaraes, Michelle Pinault, Stephane Chevalier, Nathalie Giglioli-Guivarc'h, Eric Ducos
Summary: Protein farnesylation, regulated by the ERA1 gene, plays a crucial role in Arabidopsis, impacting seed development and quality. The era1 mutants exhibit severe pleiotropic phenotypes, affecting seed traits of agronomic interest.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Xianpu Wang, Lili Xu, Xiuxia Liu, Li Xin, Shujing Wu, Xuesen Chen
Summary: The transient expression system developed for apple protoplasts shows that the AtUBQ10 and MdUBQ10 promoters are more efficient in driving gene expression compared to the CaMV 35S promoter. Using this system, it was demonstrated that active AtMKK7ac could activate signaling cascades and regulate phosphorylation and stability of MdWRKY33 in apple. The system also allowed for the reconstitution of ligand-induced interactions and regulation of AvrRpt2 stability involving auxin and the immune regulator RIN4.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Oyeyemi O. Ajayi, Michael A. Held, Allan M. Showalter
Summary: The study investigated the biochemical and physiological roles of three Golgi-localized beta-glucuronosyltransferase genes in Arabidopsis, showing alterations in the structure and content of AGPs in mutant plants. These mutations resulted in tissue-specific changes, especially in arabinose and galactose sugars, highlighting the importance of these genes in plant growth and development.
Article
Biochemistry & Molecular Biology
Joan Calle Garcia, Anna Guadagno, Andreu Paytuvi-Gallart, Alfonso Saera-Vila, Ciro Gianmaria Amoroso, Daniela D'Esposito, Giuseppe Andolfo, Riccardo Aiese Cigliano, Walter Sanseverino, Maria Raffaella Ercolano
Summary: The PRGdb database has been significantly expanded and upgraded, providing more data and tools, with a new section dedicated to collecting plant resistance transcriptomic experimental data. Additionally, the DRAGO3 tool has been improved in accuracy and sensitivity, leading to more reliable predictions.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Paz Merelo, Irene Gonzalez-Cuadra, Cristina Ferrandiz
Summary: Proliferative arrest in monocarpic plants is an evolutionary adaptation to ensure nutrient availability for seed production. The repression of cytokinin signaling precedes the proliferative arrest, and molecular changes such as the repression of known cytokinin downstream factors are associated with this process.
Article
Plant Sciences
Wanwan Zhu, Liu Yang, Di Wu, Qingcai Meng, Xiao Deng, Guoqiang Huang, Jiao Zhang, Xiaofei Chen, Cristina Ferrandiz, Wanqi Liang, Ludovico Dreni, Dabing Zhang
Summary: This study identifies the important roles of OsMADS5 and OsMADS34 in rice panicle development, limiting branching and promoting the transition to spikelet meristem identity. Through the regulation of RCN4 expression, the research reveals the mechanisms of OsMADS family in rice panicle development.
Review
Plant Sciences
Michela Osnato, Ignacio Cota, Poonam Nebhnani, Unai Cereijo, Soraya Pelaz
Summary: Fluctuations in environmental conditions greatly impact plant development, particularly changes in photoperiod. Many developmental processes are affected by the length of light/dark cycles, and they employ similar gene networks for regulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Julieta L. Mateos, Sabrina E. Sanchez, Martina Legris, David Esteve-Bruna, Jeanette C. Torchio, Ezequiel Petrillo, Daniela Goretti, Noel Blanco-Tourinan, Danelle K. Seymour, Markus Schmid, Detlef Weigel, David Alabadi, Marcelo J. Yanovsky
Summary: Plants undergo transcriptome reprograming to adapt to daily and seasonal fluctuations in light and temperature conditions. The proper spliceosome assembly, which requires the methylosome complex, is crucial for efficient pre-mRNA splicing. PICLN, a part of the methylosome complex, plays a key role in regulating pre-mRNA splicing and mediating plant adaptation to environmental conditions.
Article
Plant Sciences
Yusdar Mustamin, Turgut Yigit Akyol, Max Gordon, Andi Madihah Manggabarani, Yoshiko Isomura, Yasuko Kawamura, Masaru Bamba, Cranos Williams, Stig Uggerhj Andersen, Shusei Sato
Summary: This research found that winter survival was the direct target of selection during colonization of Japan by Lotus japonicus. Two candidate genes, FERONIA-receptor like kinase (LjFER) and a S-receptor-like kinase gene (LjLecRK), were identified to be required for non-acclimated freezing tolerance and exhibited haplotype-dependent cold-responsive expression. The study suggests that incorporating a conserved growth regulator gene, FER, and a receptor-like kinase gene, LecRK, into the cold-responsive genes contributed to freezing tolerance and local climate adaptation in L. japonicus, providing insights into the genetic mechanisms of perennial herb evolution.
Article
Multidisciplinary Sciences
Derek S. Lundberg, Roger de Pedro Jove, Pratchaya Pramoj Na Ayutthaya, Talia L. Karasov, Or Shalev, Karin Poersch, Wei Ding, Anita Bollmann-Giolai, Ilja Bezrukov, Detlef Weigel
Summary: Sphingomonas is a highly abundant bacterial genus in the phyllosphere of wild Arabidopsis thaliana, but its interaction with plants is poorly understood. Genomic analysis reveals high intergenomic diversity and adaptability of Sphingomonas, indicating frequent biotic interactions. However, plant-protective phenotypes are rare among Sphingomonas isolates.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Julia M. Kreiner, Sergio M. Latorre, Hernan A. Burbano, John R. Stinchcombe, Sarah P. Otto, Detlef Weigel, Stephen I. Wright
Summary: This study uses genome analysis of the common waterhemp to demonstrate the significant impact of modern agriculture and agricultural intensification on the evolution of agricultural weeds. The strong selection pressure and changes in allele frequency trajectories since the 1960s have influenced the success of this weed in the 21st century, with human-mediated range shifts playing a role.
Article
Cell Biology
Agustin L. Arce, Regina Mencia, Damian A. Cambiagno, Patricia L. Lang, Chang LIu, Hernan A. Burbano, Detlef Weigel, Pablo A. Manavella
Summary: Transposons are mobile elements that are silenced in order to protect genome integrity. In plants, transposable element-derived inverted repeats (IRs) are often found near genes and affect gene expression. However, the molecular mechanisms of this regulation are still unclear in most cases.
Article
Genetics & Heredity
Anjar Tri Wibowo, Javier Antunez-Sanchez, Alexander Dawson, Jonathan Price, Cathal Meehan, Travis Wrightsman, Maximillian Collenberg, Ilja Bezrukov, Claude Becker, Moussa Benhamed, Detlef Weigel, Jose Gutierrez-Marcos
Summary: Clonal propagation is commonly used in commercial plant breeding and biotechnology programs to minimize genetic variation. However, clonal plants often show stable phenotypic changes, known as somaclonal variation. Studies have linked this variation to epigenetic modifications induced during regeneration.
Review
Plant Sciences
Vicente Balanza, Paz Merelo, Cristina Ferrandiz
Summary: All flowering plants adjust their reproductive period for successful reproduction. Flower initiation is controlled by various studied factors, while the end of flowering is a less understood process at the genetic or molecular level. Recent progress in understanding the regulation of the end of flowering is presented, highlighting missing aspects for future research and potential biotechnological avenues in crop yield improvement in annual plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Sonja Kersten, Fernando A. Rabanal, Johannes Herrmann, Martin Hess, Zev N. Kronenberg, Karl Schmid, Detlef Weigel
Summary: Rapid adaptation of weeds to herbicide applications in agriculture through resistance development, particularly the grass Alopecurus myosuroides, is a common phenomenon. Target-site resistances (TSRs) are important for this adaptive response. In this study, we used PacBio HiFi reads to sequence long-range amplicons and resolved them into haplotypes using the PacBio amplicon analysis (pbaa) algorithm in pools of individuals. We recovered most haplotypes from previously sequenced individuals and found common TSR mutations originating from independent soft sweeps in new populations.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Harold Suarez-Baron, Juan F. Alzate, Barbara A. Ambrose, Soraya Pelaz, Favio Gonzalez, Natalia Pabon-Mora
Summary: This study aims to explore the genetic regulatory network underlying multicellular trichome development in Aristolochia. By comparing two different morphological species of Aristolochia, we found significant modifications in gene composition, expression, and function compared to model plants. We propose the first hypothetical genetic regulatory network for floral multicellular trichome development in Aristolochia and identify key factors responsible for the presence and specialization of floral trichomes in different species.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Letter
Biotechnology & Applied Microbiology
Herve Vanderschuren, Patience Chatukuta, Detlef Weigel, Devang Mehta
NATURE BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sami Saarenpaeae, Or Shalev, Haim Ashkenazy, Vanessa Carlos, Derek Severi Lundberg, Detlef Weigel, Stefania Giacomello
Summary: The interactions between microorganisms and their multicellular host are crucial for understanding plant or animal tissues. However, current technology hinders the study of spatial interactions between the host and its colonizing microorganisms. In this study, we introduce spatial metatranscriptomics (SmT), a sequencing-based approach that allows simultaneous characterization of host transcriptome and microbiome at a high spatial resolution. The SmT technique was applied to outdoor-grown Arabidopsis thaliana leaves and revealed tissue-scale hotspots of bacteria and fungi. By analyzing networks, we investigated the spatial interactions between microorganisms from different kingdoms, as well as the host response to microbial hotspots. SmT provides a valuable approach for studying the interplay between host and microbiome.
NATURE BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Thanvi Srikant, Wei Yuan, Kenneth Wayne Berendzen, Adrian Contreras-Garrido, Hajk-Georg Drost, Rebecca Schwab, Detlef Weigel
Summary: A systematic analysis of the requirement for MET1 in different A. thaliana accessions reveals that CG methylation plays a dual role in gene expression. For many genes, CG methylation appears to canalize expression levels, masking regulatory divergence. However, for a smaller subset of genes, CG methylation increases expression diversity beyond genetically encoded differences.
