期刊
PLANT BREEDING
卷 133, 期 2, 页码 243-249出版社
WILEY
DOI: 10.1111/pbr.12140
关键词
gene cloning; perennial ryegrass; gene expression; salt stress; P5CS; proline accumulation
资金
- National Natural Science Foundation of China [31201653]
- National High Technology Research and Development Program of China [2011AA100209-2]
Proline is an important osmoprotectant in plant in response to osmotic stresses. Delta 1-pyrroline-5-carboxylate synthetase (P5CS) is a key enzyme in proline biosynthesis. In this study, two P5CS genes (PrP5CS1 and PrP5CS2) were isolated for the first time from perennial ryegrass. Expression analysis revealed that the transcript of PrP5CS1 in leaves was significantly up-regulated in two ryegrass cultivars exposed to 255mm NaCl. The up-regulated level of PrP5CS1 was higher in salt-tolerant 'Overdrive' than in sensitive 'Pizzazz'. PrP5CS2 was significantly induced in 'Overdrive' but suppressed in 'Pizzazz' by NaCl treatment. In stems, however, there was no significant transcript change for both genes under salt treatment. The proline accumulation was significantly induced in both cultivars after salt treatment, and it was higher in 'Overdrive' than in 'Pizzazz' after 2days of salt treatment. The results suggested that both genes are salt inducible and may be associated with salt-stress tolerance in perennial ryegrass.
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