期刊
PLANT BREEDING
卷 128, 期 1, 页码 92-96出版社
WILEY
DOI: 10.1111/j.1439-0523.2008.01536.x
关键词
marigold (Tagetes erecta); recessive genic male sterility gene; sequence-related amplified polymorphism; intersimple sequence repeat; sequence characterized amplified region; marker-assisted selection
资金
- Ministry of Agriculture of China [2003-Z36]
In marigold, an F-2 segregation population of 167 plants was constructed from a cross of a line (M525A) carrying the male sterility trait x an inbred line (f53f). In line M525A, the male sterility trait was controlled by the recessive gene, Tems. The intersimple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) techniques combined with bulked segregant analysis were used to develop markers linked to the trait. From a survey of the 38 ISSR primers and 170 SRAP primer combinations, only one SRAP marker that was closely linked to the target trait was identified and successfully converted into sequence characterized amplified region (SCAR) marker that was located within 2.4 cM from Tems locus. The marker was validated with five other two-type lines and in each case the male fertile plants were reliably identified. This SCAR marker therefore permits the efficient marker-assisted selection of male sterile individuals in breeding programmes of marigold and will greatly facilitate the breeding of F-1 cultivars.
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