Article
Biochemistry & Molecular Biology
Wei Dong, Dewei Wu, Caihui Wang, Ying Liu, Defeng Wu
Summary: The study found that dwarf watermelon mutants are mainly caused by a deficiency in gibberellin, leading to stunted growth in plants. Additionally, mutations in the promoter of the ClaGA20ox gene in transgenic plants can also result in dwarfism.
Article
Plant Sciences
Paula A. Giraldo, Hiroshi Shinozuka, German C. Spangenberg, Kevin F. Smith, Noel O. Cogan
Summary: This study developed an accurate and fast method for molecular characterization of genetically modified plants, using long-read sequencing with the MinION device to achieve higher resolution of transgenic events within the host genome. The method is time-efficient, simple, and cost-effective, able to detect moderate-sized secondary insertions that may have been missed previously.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Huawei Liu, Li Jiang, Zhifeng Wen, Yingjun Yang, Stacy D. Singer, Dennis Bennett, Wenying Xu, Zhen Su, Zhifang Yu, Jonathan Cohn, Hyunsook Chae, Qiudeng Que, Yue Liu, Chang Liu, Zongrang Liu
Summary: Insulators in plants, like the RS2-9 insulator identified in rice, play a role in blocking enhancer-promoter interactions. The binding of O. sativa Homeobox 1 (OSH1) to RS2-9 is crucial for insulator function, suggesting a potential role for OSH1 in gene insulation and chromatin organization in plant genomes.
Article
Biochemistry & Molecular Biology
Qi Xie, Xufeng Wang, Juan He, Ting Lan, Jiayu Zheng, Yupeng Li, Jinkang Pan, Ling Lin, Junyi Zhao, Jing Li, Yu Yu, Beixin Mo, Xuemei Chen, Lei Gao, Lin Liu
Summary: miR156 and miR529 in land plants exhibit differences in mismatch-sensitive regions and precursor structures, evolving independently through divergent evolutionary patterns. They show distinct expression patterns and specific targets, indicating functional differences in plant growth and development regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Multidisciplinary Sciences
Dorina Podar, Frans J. M. Maathuis
Summary: Nutrients are scarce and valuable resources, and plants have developed mechanisms to monitor and adjust nutrient levels in order to optimize nutrient use efficiency. Identification of primary sensors is crucial for understanding how plants sense different nutrients.
Article
Biotechnology & Applied Microbiology
Irene M. Kaplow, Abhimanyu Banerjee, Chuan Sheng Foo
Summary: In this study, the researchers developed a new approach to identify the binding motifs of individual DNA binding domains (DBDs) of a transcription factor (TF). By analyzing chromatin immunoprecipitation sequencing (ChIP-seq) data, they trained a deep convolutional neural network to predict the preservation of wild-type TF binding sites in mutant TF datasets. They applied this approach to mouse CTCF ChIP-seq data and successfully identified the binding preferences of CTCF ZFs 3-11 as well as a putative GAG binding motif for ZF 1. Their findings provide new insights into the binding preferences of CTCF's DBDs.
Article
Virology
Lili Chu, Shengqiang Ye, Jiaying Wang, Duo Peng, Xiaoyan Wang, Yunguo Qian, Dabing Zhang
Summary: Duck adenovirus 3 (DuAdV-3; strain HB) isolated in this study is a mutant with several insertion and deletion events in its genome, demonstrating genetic variation among DuAdV-3 isolates.
ARCHIVES OF VIROLOGY
(2022)
Article
Biochemical Research Methods
Li Jiang, Yue Liu, Zhifeng Wen, Yingjun Yang, Stacy D. Singer, Dennis Bennett, Wenying Xu, Zhen Su, Zhifang Yu, Josh Cohn, Xi Luo, Zhongchi Liu, Hyunsook Chae, Qiudeng Que, Zongrang Liu
Summary: In this study, the researchers identified and validated an enhancer-blocking insulator called CW198 in plants. They found that this insulator can efficiently suppress the activation of enhancers in both Arabidopsis and tobacco plants. The properties of CW198 resemble those of the CTCF insulator in the mammalian genome.
TRANSGENIC RESEARCH
(2022)
Review
Environmental Sciences
Kaouthar Feki, Sana Tounsi, Moncef Mrabet, Haythem Mhadhbi, Faical Brini
Summary: The toxicity of metals negatively impacts plants' growth and productivity, leading to various disruptions. Plants employ efficient mechanisms through signaling pathways to combat this stress, involving metal transporters and metal-binding proteins for detoxification. Further research on the molecular basis of metal toxicity and tolerance mechanisms is crucial for genetic engineering to produce transgenic plants that enhance phytoremediation techniques.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Silvia de Oliveira Dorta, Lisia Borges Attilio, Odimar Zanuzo Zanardi, Joao Roberto Spotti Lopes, Marcos Antonio Machado, Juliana Freitas-Astua
Summary: The Asian citrus psyllid (ACP) is the vector of the devastating disease huanglongbing (HLB) in citrus. Previous research has shown the potential of using Bt strains containing cry/cyt genes to control ACP nymphs. This study attempted to produce transgenic sweet orange plants expressing the cry11A gene from Bt, and confirmed the presence and copy number of the transgene using PCR and Southern blot analyses. Greenhouse mortality assays showed that the tested events had effects ranging from 22% to 43% on D. citri nymphs after a five-day exposure period. This is the first report of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Hematology
Darya Deen, Falk Butter, Deborah E. Daniels, Ivan Ferrer-Vicens, Daniel C. J. Ferguson, Michelle L. Holland, Vasiliki Samara, Jacqueline A. Sloane-Stanley, Helena Ayyub, Matthias Mann, Jan Frayne, David Garrick, Douglas Vernimmen
Summary: The study identified the widely expressed protein MAZ as a transcription factor involved in regulating the expression of erythroid-specific genes and found that MAZ also plays a role in the promoters and enhancer elements of key erythroid genes. Knockdown of MAZ led to reduced alpha-globin expression and impaired differentiation of primary human erythroid cells, and genetic variants in the MAZ locus were associated with changes in important human erythroid traits. These findings reveal MAZ as a previously unrecognized regulator of the erythroid differentiation program.
Letter
Chemistry, Medicinal
Hiroyuki Fuchino, Osamu Iida, Noriaki Kawano, Nobuo Kawahara, Kayo Yoshimatsu
Summary: This study performed a discriminant LC/MS quantitative analysis of ephedrine and pseudoephedrine in Ephedrae herba, revealing that the contents of these compounds varied in different branches and internodes. The content ratio of EP and PEP reversed in some branches depending on their growth extent, and there was a strong correlation between internode distance and alkaloid contents.
JOURNAL OF NATURAL MEDICINES
(2021)
Article
Plant Sciences
Insha Amin, Saiema Rasool, Mudasir A. Mir, Wasia Wani, Khalid Z. Masoodi, Parvaiz Ahmad
Summary: Plants adapt to salt stress by activating genes and transcription factors that regulate ion transport and exclusion, which help alleviate ion toxicity in cells.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Lichao Zhang, Guanhua He, Yaping Li, Ziyi Yang, Tianqi Liu, Xianzhi Xie, Xiuying Kong, Jiaqiang Sun
Summary: The PIL family transcription factors were identified as new repressors of tillering in cereal crops, controlling tiller number in wheat and rice through interactions with TaTB1 and OsTB1. Additionally, PIL proteins interact with SPLs in different plants, playing a crucial role in inhibiting shoot branching.
Article
Biotechnology & Applied Microbiology
Jhumishree Meher, Ankita Sarkar, Birinchi Kumar Sarma
Summary: Molecular docking was used to investigate the interactions between differentially expressed rice WRKY proteins and blast resistance genes Pi2 and Pi54 under rice blast disease and drought stresses. The study revealed differential binding patterns of the WRKY proteins with the two resistance genes. Some WRKY proteins interacted with the genes involving the residues of the zinc finger structure, while others interacted involving the residues of the conserved WRKYGQK heptapeptide sequence. The findings suggest that the interaction between OsWRKY proteins and the two blast resistance genes is important for mitigating the blast challenge in rice under overlapping drought and blast stresses.
