期刊
PLANT BIOTECHNOLOGY REPORTS
卷 5, 期 3, 页码 283-287出版社
SPRINGER
DOI: 10.1007/s11816-011-0173-4
关键词
Asiaticoside; Centella asiatica; Thidiazuron; Madecassoside; Methyl jasmonate; Phytosterols
资金
- Korean Government (MOEHRD) [KRF-2007-C00271]
- Korea Evaluation Institute of Industrial Technology (KEIT) [2007NCTHME020000] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In order to produce centellosides from whole plant cultures of Centella asiatica (L.) Urban, we evaluated the synergistic effects of thidiazuron (TDZ) and methyl jasmonate (MJ) on whole plant growth and centelloside production. After 4 weeks of treatment with 0.025 mg/L of TDZ coupled with 0.1 mM MJ, the production of madecassoside and asiaticoside from whole plant cultures was estimated to be 2.40- and 2.44-fold, respectively, above that of MJ elicitation alone. When whole plants were treated with a growth regulator and an elicitor, the growth of whole plants, as compared to the controls, did not differ. Additionally, total phytosyterol content in the leaves of whole plants co-treated with MJ and TDZ was 1.08-fold greater than those of MJ alone. These results demonstrate that combined treatments not only stimulate the accumulation of centellosides in the leaves but also inhibit the reduction of phytosterol levels caused by MJ elicitation.
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