Review
Plant Sciences
Jessica N. N. Stockdale, Reginald J. J. Millwood
Summary: The adoption of genetically engineered (GE) crops has brought economic and environmental benefits, but concerns about the potential movement of transgenes beyond cultivation have been raised. Despite nearly three decades of GE crop cultivation, no widely adopted bioconfinement system exists. However, it may be necessary to implement such systems in new GE crops or those with high potential for transgene flow.
Review
Biochemistry & Molecular Biology
Wenjing Ren, Jinchao Si, Li Chen, Zhiyuan Fang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Hailong Yu, Yangyong Zhang
Summary: Hybrid production using Ogura CMS has become an important approach to harness heterosis in vegetables. This article provides an overview of the mapping, cloning, mechanism and application of Ogura CMS and fertility restorer genes in Brassica napus, Brassica rapa, Brassica oleracea and other cruciferous crops, and discusses the current challenges and future research directions in the application of Ogura CMS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Ira Vashisht, Namrata Dhaka, Rubi Jain, Akanksha Sood, Niharika Sharma, Manoj K. Sharma, Rita Sharma
Summary: Plants are constantly affected by environmental stresses, resulting in significant yield losses. As climate conditions change, the intensity and duration of these stresses are expected to increase, posing a serious threat to crop productivity. Male gametogenesis is one of the most sensitive developmental stages, and exposure to environmental stresses during this stage leads to male sterility and yield loss. Research on small RNAs, long non-coding RNAs, and their complex regulatory interactions has begun to uncover the molecular mechanisms of non-coding RNA-mediated environmental stress surveillance and regulation of male fertility in plants. Candidate ncRNAs emerging from these studies can be utilized to generate environment-sensitive male sterile lines for hybrid breeding or mitigate the impact of climate change on male fertility, as needed.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Paula A. Giraldo, Hiroshi Shinozuka, German C. Spangenberg, Kevin F. Smith, Noel O. Cogan
Summary: This study developed an accurate and fast method for molecular characterization of genetically modified plants, using long-read sequencing with the MinION device to achieve higher resolution of transgenic events within the host genome. The method is time-efficient, simple, and cost-effective, able to detect moderate-sized secondary insertions that may have been missed previously.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Konstantin Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata Ogneva, Eduard Y. Kostetsky, Alexandra S. Dubrovina
Summary: Recent investigations have shown that exogenous double-stranded RNAs (dsRNAs) can specifically suppress transgene expression in plants, indicating the potential of using dsRNAs as a promising strategy for plant gene regulation.
Article
Plant Sciences
S. Antony Ceasar, S. Ignacimuthu
Summary: Genome editing tools based on CRISPR/Cas system have the potential to solve agricultural issues and improve food production. The use of genetic engineering in agriculture has allowed for the introduction of specific traits in crops, while CRISPR/Cas system offers a more precise technique for targeted modification of genes in the host plant genome. The delivery of CRISPR reagents has the potential to overcome limitations with traditional transformation methods and produce transgene-free plants. The CRISPR/Cas system is expected to play a key role in future crop breeding.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Shun Yamazaki, Ikuo Hirano, Koichiro Kato, Masayuki Yamamoto, Norio Suzuki
Summary: The 180kb genomic fragment contains the fully functional Epo gene unit, and EPO from the liver dominantly stimulates hepatic erythropoiesis while contributing less to erythropoiesis in other organs.
Article
Agronomy
Shuangshuang Liu, Ziwen Li, Suowei Wu, Xiangyuan Wan
Summary: This review summarizes the critical role of sugar metabolism in plant male reproduction and the key genes involved in anther and pollen development. Prediction of 112 putative sugar metabolic GMS genes in maize provides valuable insights for plant pollen development and male fertility.
Article
Plant Sciences
Abhishek Bohra, Prasad Gandham, Abhishek Rathore, Vivek Thakur, Rachit K. Saxena, S. J. Satheesh Naik, Rajeev K. Varshney, Narendra P. Singh
Summary: The comparative analysis of genome-wide miRNAs and their gene targets between cytoplasmic male sterile (CMS) and fertile lines of pigeonpea suggests a possible role of miRNA-regulated pathways in reproductive development.
Article
Agronomy
Ayumu Takatsuka, Tomohiko Kazama, Kinya Toriyama
Summary: The study obtained a Tadukan-type CMS line and a restorer line through backcrossing, determined the whole-genome sequences of the TAA mitochondria, and identified the orf312 gene specific to this genome, which showed differential expression patterns at different growth stages.
Article
Biotechnology & Applied Microbiology
Yilin Jiang, Xueli An, Ziwen Li, Tingwei Yan, Taotao Zhu, Ke Xie, Shuangshuang Liu, Quancan Hou, Lina Zhao, Suowei Wu, Xinze Liu, Shaowei Zhang, Wei He, Fan Li, Jinping Li, Xiangyuan Wan
Summary: By sequencing and analyzing anther transcriptomes in different maize inbred lines, 12 new maize GMS genes were identified, providing important insight into male sterility and potential applications in maize breeding.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Hongzhan Liu, Zhongke Sun, Lizong Hu, Chaoqiong Li, Xueqin Wang, Zonghao Yue, Yulin Han, Guangyu Yang, Keshi Ma, Guihong Yin
Summary: This study conducted comparative transcriptome analysis on heat-induced male sterile anthers in wheat, revealing differences in cytological characteristics and identification of 9601 differentially expressed genes. These DEGs were associated with pathways such as starch and sucrose metabolism, phosphatidylinositol signaling, peroxidase activity, oxidative stress response, and heme binding. This comprehensive analysis provides insights into the molecular mechanisms underlying heat-induced male sterility in wheat.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Kun Xu, Jinlong Zhu, Ning Guo, Jinyu Liu, Hong Zhai, Xiaobin Zhu, Yi Gao, Hongyan Wu, Zhengjun Xia
Summary: Snap bean (Phaseolus vulgaris L.) is an important vegetable crop, and the characterization of the genic male sterility (ms-2) mutant in common bean is reported. Loss of function of MS-2 accelerates degradation of the tapetum, resulting in complete male sterility. Through analysis, the causal gene for MS-2 is identified as Phvul.003G032100, which encodes the PvTKPR2 protein.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Plant Sciences
Jingyi You, Min Li, Hongwei Li, Yulin Bai, Xuan Zhu, Xiangjun Kong, Xiaoyan Chen, Ruiyang Zhou
Summary: This study investigated the DNA methylation patterns in cytoplasmic male sterility (CMS) lines of cotton using bisulfite sequencing technology and RNA-Seq. The results showed that CMS lines had more hypermethylated genes compared to near-isogenic lines, indicating a higher susceptibility to methylation. Furthermore, differentially expressed methylated genes were mainly involved in vital metabolic pathways, and there was a negative correlation between gene methylation and expression. Additionally, five key genes potentially associated with CMS were identified.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biology
Laura Martinez-Alarcon, Sergio Liarte, Juan J. Quereda, Aida Saez-Acosta, Carlos de Torre-Minguela, Livia Mendonca, Juana M. Abellaneda, Maria J. Majado, Antonio Rios, Pablo Ramirez, Antonio Munoz, Guillermo Ramis
Summary: Xenotransplantation of pig organs has attracted attention as a solution to the imbalance between availability and needs of human organs, particularly focusing on transgenic pigs expressing human CD55 to overcome compatibility constraints. By analyzing organs from five transgenic pigs, researchers showed variations in hCD55 gene expression and hDAF protein presence in different organs, as well as the ability to withstand hyper-acute rejection. Additionally, correlations were found between hCD55 expression and DAF levels in various tissues, providing potential insights into predicting expression levels without the need for direct biopsies.
Article
Plant Sciences
Sarbottam Piya, Tracy Hawk, Bhoomi Patel, Logan Baldwin, John H. Rice, C. Neal Stewart, Tarek Hewezi
Summary: The study demonstrates that inactivating protein kinases through kinase-dead mutations can enhance soybean resistance to nematodes, showing the potential of this approach in increasing plant resistance to biotic and abiotic stresses.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Alessandro Occhialini, Alexander C. Pfotenhauer, Li Li, Stacee A. Harbison, Andrew J. Lail, Jason N. Burris, Cristiano Piasecki, Agnieszka A. Piatek, Henry Daniell, C. Neal Stewart, Scott C. Lenaghan
Summary: A novel synthetic genome structure called the 'mini-synplastome' has been designed and developed, inspired by dinoflagellate plastome organization. It can replicate, be cloned easily, express genes predictably in plastids, not integrate vector sequences into the native plastome, and persist autonomously in plants over generations without external selection pressure. This innovation is anticipated to revolutionize chloroplast biotechnology, facilitate marker-free plastid engineering, and provide an unparalleled platform for one-step metabolic engineering in plants.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Review
Plant Sciences
Mst Shamira Sultana, Reginald J. Millwood, Mitra Mazarei, C. Neal Stewart
Summary: Legume proteinase inhibitors have shown potential for plant protection against herbivores by inhibiting digestive proteinases in insects. Multiple PIs co-expressed in transgenic plants have been found to enhance host defense in an additive manner. Further research is needed to expand PI gene repertoires and improve efficacy against multiple proteinases.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Vivek Shrestha, Hari B. Chhetri, David Kainer, Yaping Xu, Lance Hamilton, Cristiano Piasecki, Ben Wolfe, Xueyan Wang, Malay Saha, Daniel Jacobson, Reginald J. Millwood, Mitra Mazarei, C. Neal Stewart
Summary: This study explored the genetic basis of nitrogen use efficiency (NUE) in switchgrass through genome wide association study (GWAS) and identified 19 unique single nucleotide polymorphisms (SNPs) and 32 candidate genes related to NUE. The research provides potential avenues for the development of improved nitrogen use-efficient switchgrass lines.
FRONTIERS IN PLANT SCIENCE
(2022)
Editorial Material
Multidisciplinary Sciences
Fred Gould, Richard M. Amasino, Dominique Brossard, C. Robin Buell, Richard A. Dixon, Jose B. Falck-Zepeda, Michael A. Gallo, Ken E. Giller, Leland L. Glenna, Timothy Griffin, Daniel Magraw, Carol Mallory-Smith, Kevin V. Pixley, Elizabeth P. Ransom, David M. Stelly, C. Neal Stewart
Review
Biotechnology & Applied Microbiology
Yongil Yang, Timothy Alexander Chaffin, Amir H. Ahkami, Eduardo Blumwald, Charles Neal Stewart
Summary: Plant-based biosynthesis plays a crucial role in environmental sustainability by reducing greenhouse gas emissions, water pollution, and biodiversity loss. Recent advancements in genetic editing, synthetic promoter development, and single-cell omics have improved our understanding of cell-type-specific mechanisms and enhanced bioproduction.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Robert G. Sears, Stephen B. Rigoulot, Alessandro Occhialini, Britany Morgan, Tayebeh Kakeshpour, Holly Brabazon, Caitlin N. Barnes, Erin M. Seaberry, Brianna Jacobs, Chandler Brown, Yongil Yang, Tayler M. Schimel, Scott C. Lenaghan, C. Neal Stewart
Summary: Nuclear energy is expected to grow in order to meet climate goals, leading to an increased need to detect leakage from nuclear plants and assess their impact on ecosystems. Mechanical sensors currently used to detect gamma radiation have limitations, prompting the development of a plant biosensor that utilizes synthetic biology to detect low-dose ionizing radiation. The radiation phytosensor has shown promising results in detecting gamma radiation exposure and functioning effectively in real-world scenarios.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Mst Shamira Sultana, Mitra Mazarei, Juan Luis Jurat-Fuentes, Tarek Hewezi, Reginald J. J. Millwood, C. Neal Stewart
Summary: Trypsin inhibitors (TIs) in plants play a protective role against herbivores by reducing the biological activity of trypsin. Soybean contains two major TI classes, Kunitz trypsin inhibitor (KTI) and Bowman-Birk inhibitor (BBI). This study investigated the potential role of soybean TIs in plant defense against insects and nematodes. Overexpression of TI genes in soybean and Arabidopsis led to significant increase in trypsin and chymotrypsin inhibitory activities and reduced larvae weight of corn earworm (Helicoverpa zea). However, no differences were observed in soybean cyst nematode (SCN) female index between transgenic and non-transgenic plants. The study highlights the potential applications of TI genes for insect resistance improvement in plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Mitra Mazarei, Pratyush Routray, Sarbottam Piya, C. Neal Stewart Jr, Tarek Hewezi
Summary: In this study, a transcriptome analysis of RNA-seq data was performed to select candidate genes associated with root growth and development in soybean. Overexpression of the GmNAC19 and GmGRAB1 transcription factors in transgenic composite plants significantly increased root growth, biomass, and seed yield. Additionally, GmNAC19 overexpression enhanced water stress tolerance in the transgenic plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Alexander C. Pfotenhauer, Alessandro Occhialini, Stacee A. Harbison, Li Li, Agnieszka A. Piatek, Curtis R. Luckett, Yongil Yang, C. Neal Stewart, Scott C. Lenaghan
Summary: Genome editing technology has been used to improve staple food crops, such as potato, by increasing their yield. In this study, CRISPR/Cas9 was used to edit the genome of potato and successfully increased the size of starch granules in tubers, resulting in increased yield. The results of this study demonstrate the enormous potential of genome editing technology for crop improvement.
Article
Plant Sciences
Zheng-Jun Guan, Min Zheng, Zhi-Xi Tang, Wei Wei, C. Neal Stewart Jr
Summary: In this study, differential expression of total protein in leaves of two transgenic oilseed rape lines and their non-transgenic parent plant was analyzed. Fourteen differential protein spots were identified, including eleven upregulated and three downregulated proteins, involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. These changes in protein spots may be attributed to the insertion of foreign transgenes in transgenic oilseed rape, but transgenic manipulation does not necessarily cause significant changes in the proteomes of oilseed rape.
Article
Plant Sciences
Britany Lauren Morgan, Tayebeh Kakeshpour, Alessandro Occhialini, Gabriella King, Megan Sichterman, Stacee A. Harbison, Stephen B. Rigoulot, Holly Brabazon, Charles Neal Stewart Jr, Scott C. Lenaghan
Summary: The heterologous expression of the Escherichia coli OtsB gene in potato significantly improves the plant's resilience to various stress factors, including heat, photoperiod, herbivory, and competition. This increase in stress tolerance leads to higher tuber yield and overall stability in crop plants.
Editorial Material
Plant Sciences
Wei Wei, Charles Neal Stewart Jr
Article
Plant Sciences
Mst Shamira Sultana, Mitra Mazarei, Reginald J. Millwood, Wusheng Liu, Tarek Hewezi, C. Neal Stewart Jr
Summary: This study developed synthetic promoters inducible by soybean cyst nematode (SCN) and showed their functionality in transgenic soybean plants. The synthetic promoters specifically directed gene expression to roots in response to nematode infection but not in leaves. The study also examined the specificity of the synthetic promoters to various biotic and abiotic stresses as well as signal molecules.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
