Article
Biochemical Research Methods
Josie L. McQuillan, Anthony J. Berndt, Ashley E. Sproles, Stephen P. Mayfield, Jagroop Pandhal
Summary: In this study, novel genetic devices for transgene expression in Chlamydomonas reinhardtii have been developed by identifying cis-regulatory DNA elements capable of driving high transgene expression. This provides a new toolbox of cis-regulatory elements for driving transgene expression at various levels and reveals insights into promoter structure and gene regulation in C. reinhardtii.
Review
Microbiology
Edoardo Andrea Cutolo, Giulia Mandala, Luca Dall'Osto, Roberto Bassi
Summary: Photosynthetic microbes are gaining attention as hosts for low-cost production of high-value recombinant proteins. Manipulation of unicellular algal genomes offers the opportunity to establish engineered strains as alternatives to conventional expression systems. Small-sized algal chloroplasts are excellent targets for synthetic biology approaches and compartmentalized accumulation of products. Expression of recombinant proteins, including therapeutic enzymes and peptides, has been successfully achieved in the chloroplasts of model organism Chlamydomonas reinhardtii and other species, demonstrating the potential of transplastomic algal biotechnology.
Article
Biotechnology & Applied Microbiology
Noam Shahar, Tamar Elman, Rosalind Williams-Carrier, Oren Ben-Zvi, Iftach Yacoby, Alice Barkan
Summary: The genomes of microalgal chloroplasts have advantages for expressing foreign proteins, but efficient systems are needed to regulate their expression. Studies show that nucleus-encoded chloroplast gene activators from land plants can activate chloroplast transgenes in microalgae. However, limited activation may be due to suboptimal expression of the nuclear transgenes.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Genetics & Heredity
Jihye Yeon, Stephen M. M. Miller, Wipawee Dejtisakdi
Summary: Microalgae are a promising platform for producing valuable commercial products, and this study developed synthetic operon vectors to express multiple proteins from a single chloroplast transcription unit. The results showed successful expression of two proteins, but some coding sequences did not work well in synthetic operons in this alga.
Review
Biotechnology & Applied Microbiology
Ke Ma, Lei Deng, Haizhen Wu, Jianhua Fan
Summary: Microalgae are widely distributed organisms in nature that play a vital role in reducing industrial carbon emissions. They have the unique advantage of being able to carry out photosynthesis and harbor both prokaryotic and eukaryotic expression systems, making them a popular host for recombinant proteins. This article focuses on the latest technological progress in selectable markers for genetic engineering of microalgae, particularly the model species Chlamydomonas reinhardtii. The development of genetic tools and strategies has made it more feasible to develop microalgae chloroplasts as green factories for producing recombinant proteins. However, further techniques and toolkits need to be developed for efficient and markerless editing of plastid genomes.
BIORESOURCES AND BIOPROCESSING
(2022)
Review
Plant Sciences
Harry O. Jackson, Henry N. Taunt, Pawel M. Mordaka, Alison G. Smith, Saul Purton
Summary: In order to provide sustainable and economically viable support for the growing global population, a paradigm shift in agricultural productivity is necessary, including the use of biotechnology to produce future crop plants. Genetic engineering methods aimed at enhancing photosynthetic efficiency or altering the composition of harvested tissues may need to be replaced by new-to-nature pathways for significant step changes. Utilizing synthetic metabolism and reprogramming the metabolism of chloroplasts in plants through chloroplast and nuclear engineering may be essential for future advancements in agricultural productivity.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Engineering, Chemical
Huiling Wei, Mengyue Wu, Aili Fan, Haijia Su
Summary: Trichoderma is a widely distributed fungal genus in soils with high extracellular secretion capability, making it an interesting host for protein production. By summarizing and classifying strategies for protein production and strain engineering, Trichoderma spp. show potential to become successful hosts for producing desired recombinant proteins with higher yields and purity. With advanced biotechnologies and available expression platforms, Trichoderma spp. could be utilized more effectively for recombinant protein production.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Review
Plant Sciences
Ryan J. Coates, Mark T. Young, Simon Scofield
Summary: Recombinant proteins play a crucial role in research, industry, and medicine. Transgenic plants have emerged as viable and preferable options for producing recombinant proteins due to their low cost, scalability, and ability to produce proteins with complex post-translational modifications. This review focuses on the recent progress in producing recombinant proteins in plants and highlights the factors that need to be considered for optimizing expression and purification of target proteins.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Engineering, Chemical
Ace Bryan Sotelo Cabal, Tzong-Yuan Wu
Summary: This review article discusses the devastation caused by coronaviruses in both human and animal populations and emphasizes the importance of science-based approaches, particularly biotechnology, in overcoming the health and economic threats posed by coronaviruses. The article provides an overview of the genome, morphology, and key structural proteins of different coronaviruses and highlights the various recombinant protein technology systems used for the development of countermeasures against coronaviruses.
Article
Multidisciplinary Sciences
Marius Arend, Yizhong Yuan, M. Aguila Ruiz-Sola, Nooshin Omranian, Zoran Nikoloski, Dimitris Petroutsos
Summary: Using a transcriptomic data compendium of Chlamydomonas reinhardtii, we reconstructed a gene regulatory network and identified transcriptional regulators of photoprotection. Our study showed that the CCM regulator LCR1 also controls photoprotection, and QER7, a Squamosa Binding Protein, suppresses photoprotection and CCM-gene expression under the control of Phototropin. This study provides insights into gene expression regulation in microalgae.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Sanghun Baek, Sousuke Imamura, Takeshi Higa, Yumi Nakai, Kan Tanaka, Masato Nakai
Summary: This study reveals the involvement of similar translocon complexes in chloroplast protein import in red algae, and identifies a class of GTP-binding proteins that may function as plastid targeting factors in Rhodophyta.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Maham Akram, Mohsin Ahmad Khan, Nadeem Ahmed, Rashid Bhatti, Rabbia Pervaiz, Kausar Malik, Saad Tahir, Rabia Abbas, Fareeha Ashraf, Qurban Ali
Summary: In this study, researchers evaluated the algae chloroplast expression system for the recombinant production of an anti-cancer therapeutic protein (IL29). The results showed that the algae-expressed IL29 was biologically active in an anti-proliferating bioassay using HepG2 cells, indicating that the algae expression system is convenient, low-cost, eco-friendly, and safe.
Review
Biotechnology & Applied Microbiology
Dominic R. Whittall, Katherine Baker, Rainer Breitling, Eriko Takano
Summary: Spider silk is known for its impressive mechanical properties, but farming spiders for silk is impractical. Research focuses on producing recombinant spider silk proteins in more feasible hosts, with the goal of developing a highly efficient and cost-effective production system. Genes encoding these proteins have been successfully expressed in various host platforms, showing potential for large-scale production.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Oliver D. Caspari
Summary: The study reveals that additional sequence stretches are required for efficient chloroplast import of heterologous cargo proteins in Chlamydomonas chloroplast transit peptides, beyond the cleavage site.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Deserah D. D. Strand, Berkley J. J. Walker
Summary: Humans have been using biology to produce valuable compounds for a long time, and biotechnology has the potential to solve urgent global problems. However, the stoichiometry of ATP and reducing equivalents produced by photosynthesis may not match the demands of synthetic metabolism, leading to inefficiency or damage. This review discusses the role of photosynthesis in the biotech industry and the energetic considerations of using it for synthetic biology.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)