Article
Agricultural Engineering
Youngchul Yoo, Yu-Ri Oh, Gyeong Tae Eom
Summary: In this study, waste office paper was valorized into cellobionic acid using a two-step biocatalytic method. The researchers successfully optimized the reaction conditions and achieved a high yield of cellobiose and CBA. This study lays the groundwork for low-cost production of CBA and has the potential to expand its application in industries.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Xinxin Li, Xusheng Yan, Meiying Ye, Chuang Song, Jingsi Gao, Jia Zhu, Yanping Liu
Summary: In this study, NaOH-cellulase pretreatment was used to effectively improve the anaerobic digestion (AD) of corn straw. The optimal conditions for NaOH-cellulase pretreatment were found to be 6.0% NaOH concentration and 0.10 g.g(-1) dry matter cellulase. NaOH-cellulase pretreatment significantly increased methane production and straw degradation rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Thermodynamics
Hao Sha, Bo Zhao, Yuyi Yang, Yanhui Zhang, Pengfei Zheng, Shengxian Cao, Qing Wang, Gong Wang
Summary: The combination of magnetized cellulase and Ni-graphite coated materials significantly increased CH4 yield and influenced microbial community composition when treating corn stover. Carbon balance calculations showed that the combination treatment resulted in higher energy conversion efficiency.
Article
Biochemistry & Molecular Biology
Mingtang Tan, Zhaoyang Ding, Dazhang Yang, Jing Xie
Summary: This study investigated the effects of 1% cellobiose and 0.5 and 1% carboxylated cellulose nanofibers on ice crystal growth and recrystallization in frozen large yellow croaker fillets, finding that denser and more uniformly distributed ice crystals were observed in treated samples. Additionally, the addition of these substances suppressed bound water conversion, stabilized protein structure, and limited conformational transitions, highlighting the potential of cellobiose and carboxylated cellulose nanofibers as novel cryoprotectants.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Gastroenterology & Hepatology
Meixia Li, Yeqing Wang, Ciliang Guo, Sheng Wang, Liangzhen Zheng, Yifan Bu, Kan Ding
Summary: A demonstration of cellulose degrading bacterium from human gut challenged the belief that humans cannot degrade cellulose. However, research on the molecular level of cellulose degradation by human gut microbiota is still incomplete. Using cellobiose as a model, we investigated the molecular mechanism behind cellulose degradation by key members of the human gut microbiota, such as Bacteroides ovatus (BO). Our results showed that a new polysaccharide utilization locus (PUL) from BO was involved in cellobiose capturing and degradation, and two new cellulases on the cell surface, BACOVA_02626 (GH5) and BACOVA_02630 (GH5), were responsible for cellobiose degradation into glucose. Studies on mice also revealed that cellobiose reshaped the gut microbiota composition and potentially modified bacterial metabolic functions. These findings provide further evidence of cellulose degradation by human gut microbes and contribute new insights to cellulose research.
Article
Agricultural Engineering
Fanfan Ran, Xia Wang, Huaxiao Li, Huihui Zhang, Jinhui Pang, Zhiping Wang, Lu Li
Summary: The in-situ homogeneous enzymolysis of cellulose in a supercritical carbon dioxide system using cellulase modified with dextran has been shown to effectively hydrolyze cellulose and produce high concentrations of reducing sugar. This method holds great significance for sustainable utilization of cellulose.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Dewi Sartika, Amanda Patappari Firmansyah, Isnam Junais, I. Wayan Arnata, Farah Fahma, Afrinal Firmanda
Summary: In this study, corn cob was successfully converted into nanocrystalline cellulose, which has potential applications in polymer composites due to its high thermal stability. The method used was simple, cost-effective, environmentally friendly, and yielded high purity cellulose.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Energy & Fuels
Archontoula Kalogeropoulou, Iris Plioni, Dimitra Dimitrellou, Magdalini Soupioni, Poonam Singh Nigam, Maria Kanellaki, Athnasios A. Koutinas
Summary: The study focuses on fermenting cellobiose to ethanol using a non-GMO Saccharomyces cerevisiae cell-factory, achieving high conversion rates in a one-step bioconversion process. The findings suggest the efficiency of the cell-factory in producing ethanol and present a new concept for bio-ethanol production.
Review
Biotechnology & Applied Microbiology
Guangxu Yang, Xiaolin Luo, Li Shuai
Summary: Cellulose hydrolysis is an important process for producing biobased fuels and chemicals, with solid acid catalysts showing potential advantages over conventional mineral acids. However, challenges such as slow mass transfer and lack of ideal binding sites on the catalyst surface still need to be addressed for efficient cellulose hydrolysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
K. Narayanan Rajnish, Melvin S. Samuel, Ashwini J. John, Saptashwa Datta, Narendhar Chandrasekar, Ramachandran Balaji, Sujin Jose, Ethiraj Selvarajan
Summary: Cellulose as a raw material for biofuel production requires breakdown into intermediate sugars, and immobilizing cellulase enzymes on nanomaterials has been proven to significantly enhance efficiency and provide an economic solution for the processing of cellulose.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Feitian Bai, Tengteng Dong, Zheng Zhou, Wei Chen, Chenchen Cai, Xusheng Li
Summary: This study demonstrates a strategy of intergranular swelling, hydrolysis, and oxidation to overcome the biological barriers in cellulose. The pretreatment of bagasse significantly removes lignin and cellulose, increases the specific surface area and pore size, and improves the glucose recovery in enzymolysis.
Article
Energy & Fuels
Zhiwei Song, Xuechen Wen, Tao Sheng, Caiyu Sun
Summary: In this study, pretreatment of corn stalks with Aspergillus fumigatus CLL was found to significantly enhance saccharification by not only degrading lignin but also complementing the incomplete cellulase system, resulting in a significant increase in sugar yields.
BIOENERGY RESEARCH
(2022)
Article
Chemistry, Applied
Xia Li, Yishan Kuang, Yifei Jiang, Hui Dong, Wenjia Han, Qijun Ding, Jiang Lou, Yueying Wang, Tingting Cao, Jun Li, Wenjuan Jiao
Summary: The effect of three different nanocelluloses (varying in crystalline structure and morphology) on lipid digestion in vitro was investigated. It was found that both the crystalline structure and morphology of nanocellulose influenced the degree of lipid digestion, with morphology having a greater effect. The release of free fatty acids in the small intestine was ranked in the order of CNCs-I, CNCs-II, and CNFs. These findings suggest that using nanocellulose as a food-grade particle stabilizer can modulate the digestion of Pickering emulsified lipids, which is beneficial for the development of functional foods.
CARBOHYDRATE POLYMERS
(2022)
Article
Microbiology
Zedong Jiang, Liufei Long, Meifang Liang, Hebin Li, Yanhong Chen, Mingjing Zheng, Hui Ni, Qingbiao Li, Yanbing Zhu
Summary: In this study, a new beta-glucosidase MaGlu1A was cloned from a marine bacterium, showing optimal activity at 40 degrees C and pH 4.5, substrate preference for aryl-beta-glycosidic bonds with glucose, fucose, and galactose moieties, and strong stimulation by supplemental glucose. The enzymatic characterization of MaGlu1A provides valuable information for its practical applications.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Agricultural Engineering
Zhiwei Wang, Haoran Tang, Gongwei Liu, Hanxuan Gong, Yangguang Li, Yulin Chen, Yuxin Yang
Summary: Low-value agricultural by-products can be converted into high-value biological products through fermentation or enzymatic hydrolysis. This study investigated the solid-state fermentation of millet bran using a cellulase preparation and compound probiotics producing cellulase (CPPC). The results showed that both factors effectively reduced the crude fiber content and increased the contents of beneficial metabolites and microorganisms. CPPC had higher effectiveness in reducing anti-nutrient factors and increasing anti-inflammatory metabolites. The correlation analysis revealed a synergistic growth between Lactiplantibacillus and Issatchenkia during fermentation. These findings provide a theoretical reference for the efficient utilization of agricultural by-products.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)