Review
Plant Sciences
Srividhya Venkataraman, Imran Khan, Peyman Habibi, Maria Le, Rory Lippert, Kathleen Hefferon
Summary: Plants have been explored as a platform for producing pharmaceutical proteins for over 20 years. This review article focuses on the expression and downstream purification processes for plant-made vaccines. The advantages of using plants for vaccine production, such as cost-effectiveness, ease of scaling up, and the ability to produce complex therapeutic proteins, are discussed.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Plant Sciences
Omayra C. Bolanos-Martinez, Richard Strasser
Summary: Human polioviruses are highly infectious and mainly spread through fecal-oral route. Current vaccines, which are injectable inactivated polio vaccine (IPV) and live-attenuated oral polio vaccine (OPV), have limitations in terms of reduced protection or safety concerns. Plant-based poliovirus vaccines have shown promising results as a safe and low-cost alternative. However, improvements in antigen production are needed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Matthew J. McNulty, Anton Schwartz, Jesse Delzio, Kalimuthu Karuppanan, Aaron Jacobson, Olivia Hart, Abhaya Dandekar, Anatoli Giritch, Somen Nandi, Yuri Gleba, Karen A. McDonald
Summary: The virus-based immunosorbent nanoparticle technology is a promising approach for biosensing and therapeutic antibody purification. It utilizes plant virions as chassis for diverse morphologies and high yield manufacturing. By consolidating the pro-vector system into a single vector, this study explores the potential of virus-based immunosorbent nanoparticles for therapeutic protein purification. The results demonstrate improved processing scheme and magnetic separation method for increased binding capacity and purity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Plant Sciences
Hang Su, Andre van Eerde, Espen Rimstad, Ralph Bock, Norica Branza-Nichita, Igor A. Yakovlev, Jihong Liu Clarke
Summary: Plants are valuable sources of food, feed, herbal medicines, and raw materials for industry. They can also serve as green factories for producing biopharmaceuticals and vaccines. Plant-based production platforms offer advantages such as scalability, cost-effectiveness, and safety. Recent developments include plant-made vaccines for COVID-19 and Ebola, as well as clinical trials for plant-made influenza vaccines.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Matthew J. McNulty, Naomi Hamada, Jesse Delzio, Liber McKee, Somen Nandi, Marjorie L. Longo, Karen A. McDonald
Summary: Advancements in understanding and engineering of virus-based nanomaterials (VBNs) for biomedical applications have led to the exploration of interfaces between VBNs and other biomedically-relevant chemistries and materials. The immobilization of antibody-binding VBN in silica sol-gel has shown promising results for bioseparations and biosensing applications.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Maria Lobato Gomez, Xin Huang, Derry Alvarez, Wenshu He, Can Baysal, Changfu Zhu, Victoria Armario-Najera, Amaya Blanco Perera, Pedro Cerda Bennasser, Andera Saba-Mayoral, Guillermo Sobrino-Mengual, Ashwin Vargheese, Rita Abranches, Isabel Alexandra Abreu, Shanmugaraj Balamurugan, Ralph Bock, Johannes. F. Buyel, Nicolau B. da Cunha, Henry Daniell, Roland Faller, Andre Folgado, Iyappan Gowtham, Suvi T. Hakkinen, Shashi Kumar, Sathish Kumar Ramalingam, Cristiano Lacorte, George P. Lomonossoff, Ines M. Luis, Julian K. -C. Ma, Karen. A. McDonald, Andre Murad, Somen Nandi, Barry O'Keef, Kirsi-Marja Oksman-Caldentey, Subramanian Parthiban, Mathew J. Paul, Daniel Ponndorf, Elibio Rech, Julio C. M. Rodrigues, Stephanie Ruf, Stefan Schillberg, Jennifer Schwestka, Priya S. Shah, Rahul Singh, Eva Stoger, Richard M. Twyman, Inchakalody P. Varghese, Giovanni R. Vianna, Gina Webster, Ruud H. P. Wilbers, Teresa Capell, Paul Christou
Summary: Infectious diseases, caused by pathogens or parasites, pose a significant burden on global healthcare systems. Traditional approaches like vaccination and hygiene are limited by cost and scalability, particularly in developing countries. Molecular farming offers a promising solution to address these limitations and improve healthcare access worldwide.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Review
Plant Sciences
Subramanian Parthiban, Thandarvalli Vijeesh, Thashanamoorthi Gayathri, Balamurugan Shanmugaraj, Ashutosh Sharma, Ramalingam Sathishkumar
Summary: Recombinant biopharmaceuticals have various applications in vaccines, diagnostics, and therapeutics, with plant molecular pharming being a robust platform for their large-scale production. Despite advantages such as humanized expression and reduced risk of contamination, the plant system faces challenges like non-human post-translational modifications and protein instability. Artificial intelligence (AI) can play a crucial role in overcoming these hindrances and increasing production yield and stability. By using AI-based approaches in plant-based glycan engineering, protein folding, stability, catalytic activity, and organelle targeting can be optimized. This review highlights the importance of integrating systems engineering approaches and AI-based machine learning and deep learning algorithms in protein engineering to augment protein production in plant systems.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Wenshu He, Can Baysal, Maria Lobato Gomez, Xin Huang, Derry Alvarez, Changfu Zhu, Victoria Armario-Najera, Aamaya Blanco Perera, Pedro Cerda Bennaser, Andrea Saba-Mayoral, Guillermo Sobrino-Mengual, Ashwin Vargheese, Rita Abranches, Isabel Alexandra Abreu, Shanmugaraj Balamurugan, Ralph Bock, Johannes F. Buyel, Nicolau B. da Cunha, Henry Daniell, Roland Faller, Andre Folgado, Iyappan Gowtham, Suvi T. Hakkinen, Shashi Kumar, Ramalingam Sathish Kumar, Cristiano Lacorte, George P. Lomonossoff, Ines M. Luis, Julian K.-C. Ma, Karen A. McDonald, Andre Murad, Somen Nandi, Barry O'Keef, Subramanian Parthiban, Mathew J. Paul, Daniel Ponndorf, Elibio Rech, Julio C. M. Rodrigues, Stephanie Ruf, Stefan Schillberg, Jennifer Schwestka, Priya S. Shah, Rahul Singh, Eva Stoger, Richard M. Twyman, Inchakalody P. Varghese, Giovanni R. Vianna, Gina Webster, Ruud H. P. Wilbers, Paul Christou, Kirsi-Marja Oksman-Caldentey, Teresa Capell
Summary: The fight against infectious diseases often focuses on epidemics and pandemics, but the majority of deaths occur in endemic zones, particularly in developing countries, placing a disproportionate burden on underfunded health systems. Challenges in vaccine provision are hampered by high costs, limited scalability, distribution, and storage issues, particularly in regions without cold chain infrastructure. Molecular farming, particularly using edible plants for oral drugs, shows promise in addressing these challenges and recent advancements have shown success in clinical trials and FDA approvals for orally delivered protein drugs.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Review
Biotechnology & Applied Microbiology
Juho Lee, Seon-Kyeong Lee, Jong-Sug Park, Kyeong-Ryeol Lee
Summary: The production of pharmaceutical compounds in plants is gaining attention due to their cost-effectiveness, safety, and scalability compared to other expression systems. This review discusses the history and current status of plant-made pharmaceuticals, emphasizing the importance of selecting suitable plant species and transformation technologies. While tobacco has been the most commonly used plant for pharmaceutical production, other species and methods have also been explored. However, issues regarding glycosylation and limited clinical data have hindered the widespread utilization of such pharmaceuticals, while low production rates pose challenges in achieving adequate immune responses.
PLANT BIOTECHNOLOGY REPORTS
(2023)
Article
Immunology
Abel Ramos-Vega, Elizabeth Monreal-Escalante, Eric Dumonteil, Bernardo Banuelos-Hernandez, Carlos Angulo
Summary: Plant-made vaccines have been shown to be a cost-effective and successful platform for producing and delivering vaccines against parasitic diseases. The technology has the potential to play a significant role in fighting neglected tropical diseases like Chagas disease.
EXPERT REVIEW OF VACCINES
(2021)
Review
Biotechnology & Applied Microbiology
J. W. Huebbers, J. F. Buyel
Summary: The biopharmaceutical market is driven by recombinant proteins, mainly for vaccines and antibodies. Plant molecular farming as an alternative production method offers scalability and cost efficiency in upstream processes. However, lack of standardized process equipment has hindered approvals for pharmaceutical proteins from whole plants.
BIOTECHNOLOGY ADVANCES
(2021)
Review
Biotechnology & Applied Microbiology
M. Knoedler, J. F. Buyel
Summary: Molecular farming in plants is becoming a competitive platform for producing pharmaceutical proteins, with advantages in cost and technology over established hosts like bacteria and mammalian cells. Plants can produce a variety of recombinant therapeutic proteins, including replacement enzymes, vaccines, and monoclonal antibodies, and this review focuses on the production of active antibody-toxin fusion proteins (RITs) in plants.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Plant Sciences
Miguel Angel Alcalde, Diego Hidalgo-Martinez, Roque Bru Martinez, Susana Selles-Marchart, Mercedes Bonfill, Javier Palazon
Summary: Recent advancements in plant biotechnology have highlighted the potential of hairy roots as a biotechnological platform. This study focuses on the development of hairy roots in C. asiatica and explores the optimization of genetic transformation for enhanced bioactive compound production. The proteomic analysis reveals the importance of ornithine cyclodeaminase as a traceable biomarker for centelloside production capacity.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Philippe Jutras, Riccardo Soldan, Isobel Dodds, Mariana Schuster, Gail M. Preston, Renier A. L. van der Hoorn
Summary: A bioluminescent strain of Agrobacterium tumefaciens GV3101 was generated to monitor luminescence during agroinfiltration, providing a versatile and non-destructive imaging tool for studying plant responses to Agrobacterium infiltration.
Article
Evolutionary Biology
Carrie Louise Alderley, Samuel Terrence Edwards Greenrod, Ville-Petri Friman
Summary: This study finds that biofumigation can suppress the growth of Ralstonia solanacearum plant bacterial pathogen, but under low transfer frequency exposure, the pathogen evolves tolerance to allyl-ITC plant allelochemical and develops cross-tolerance to ampicillin beta-lactam antibiotic. The tolerance evolution is linked to insertion sequence movement in multiple genes.
EVOLUTIONARY APPLICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)