期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 7, 期 7, 页码 602-610出版社
WILEY
DOI: 10.1111/j.1467-7652.2009.00427.x
关键词
acyl-CoA-binding protein; Arabidopsis; lysophosphatidylcholine acyltransferase; phosphatidylcholine; polyunsaturated fatty acid; triacylglycerol
资金
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs Program
P>The gene encoding a 10-kDa acyl-CoA-binding protein (ACBP) from Brassica napus was over-expressed in developing seeds of Arabidopsis thaliana. Biochemical analysis of T-2 and T-3 A. thaliana seeds revealed a significant increase in polyunsaturated fatty acids (FAs) (18:2(cis delta 9,12) and 18:3(cis delta 9,12,15)) at the expense of very long monounsaturated FA (20:1(cis delta 11)) and saturated FAs. In vitro assays demonstrated that recombinant B. napus ACBP (rBnACBP) strongly increases the formation of phosphatidylcholine (PC) in the absence of added lysophosphatidylcholine in microsomes from delta YOR175c yeast expressing A. thaliana lysophosphatidylcholine acyltransferase (AthLPCAT) cDNA or in microsomes from microspore-derived cell suspension cultures of B. napus L. cv. Jet Neuf. rBnACBP or bovine serum albumin (BSA) were also shown to be crucial for AthLPCAT to catalyse the transfer of acyl group from PC into acyl-CoA in vitro. These data suggest that the cytosolic 10-kDa ACBP has an effect on the equilibrium between metabolically active acyl pools (acyl-CoA and phospholipid pools) involved in FA modifications and triacylglycerol bioassembly in plants. Over-expression of ACBP during seed development may represent a useful biotechnological approach for altering the FA composition of seed oil.
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