Article
Plant Sciences
Huabin Liu, Qiong Luo, Chao Tan, Jia Song, Tan Zhang, Shuzhen Men
Summary: Auxin plays an important role in the coordinated development of the embryo, endosperm, and seed coat during seed development. In this study, the distribution pattern and function of auxin during Arabidopsis seed development were investigated. It was found that auxin response signals exhibited a dynamic distribution pattern, with strong signals observed at specific locations in the seed coat. Auxin biosynthesis genes and auxin carriers were identified as contributing factors to the accumulation and polar distribution of auxin in the seed coat. Furthermore, the accumulation of auxin in the seed coat was found to regulate seed size. These findings provide valuable insights and tools for improving crop yields.
Article
Biochemistry & Molecular Biology
Joanna Rojek, Matthew R. Tucker, Michal Rychlowski, Julita Nowakowska, Malgorzata Gutkowska
Summary: Auxin plays a crucial role in plant development, with its transport mediated by various proteins including Rab and RGTB1. The disruption of vesicular traffic due to mutations in RGTB1 affects both pre and post-fertilization events, highlighting the importance of proper vesicular trafficking in sexual reproduction of flowering plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yan Xu, Ruibo Hu, Shengjun Li
Summary: The Arabidopsis seed coat mucilage is a polysaccharide-rich matrix synthesized by the seed coat epidermal cells. It serves as an ideal model system for plant cell wall research and has contributed to the understanding of the molecular mechanisms underlying mucilage synthesis and modification. This review summarizes the genes and regulators involved in seed coat cell differentiation, mucilage biosynthesis and modification, and secondary cell wall formation, with a focus on the transcriptional regulation of mucilage production.
Article
Plant Sciences
Charles H. Burroughs, Christopher M. Montes, Christopher A. Moller, Noah G. Mitchell, Anne Marie Michael, Bin Peng, Hyungsuk Kimm, Taylor L. Pederson, Alexander E. Lipka, Carl J. Bernacchi, Kaiyu Guan, Elizabeth A. Ainsworth
Summary: Improvements in genetics, technology, and agricultural intensification have increased soybean yields, but adverse climate conditions may hinder further progress. This study found that high temperature has a nonlinear impact on yield, with decreases observed when canopy temperature exceeds a threshold. In the US Midwest, increased temperature reduces soybean yields through reduced leaf area, fewer pods per node, smaller seeds, and decreased harvest index.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Joan Renard, Gaetano Bissoli, Maria Dolores Planes, Jose Gadea, Miguel angel Naranjo, Ramon Serrano, Gwyneth Ingram, Eduardo Bueso
Summary: This study explores the relationship between TFs AtHB25 and ICE1. The lack of ICE1 suppresses the enhanced seed longevity and impermeability of the overexpressing mutant athb25-1D, while seed coat lipid polyester deposition is not affected. Another mutant zou-4, which lacks the transcriptional program for proper endosperm maturation, also shows high sensitivity to seed aging.
Article
Plant Sciences
Zhiyang Yuan, Kai Fan, Yuntong Wang, Li Tian, Chaopu Zhang, Wenqiang Sun, Hanzi He, Sibin Yu
Summary: This study cloned and characterized the rice seed storability-related gene OsGH3-2, which was shown to affect seed storability by regulating the accumulation level of abscisic acid (ABA). Transgenic, knockout, and knockdown experiments revealed that OsGH3-2 acted as a negative regulator of seed storability by modulating genes related to the ABA pathway and late embryogenesis-abundant proteins.
Review
Plant Sciences
Muhammad Saad Rehmani, Usman Aziz, BaoShan Xian, Kai Shu
Summary: Seed dormancy and longevity play important roles in crop production, but their relationship is still unclear. Some studies suggest a positive correlation between seed dormancy and longevity, while increasing evidence highlights a negative relationship, mainly influenced by phytohormones.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Caleo Panhoca de Almeida, Isabella Laporte Santos, Jean Fausto de Carvalho Paulino, Caio Cesar Ferrari Barbosa, Cassia Cristina Augusto Pereira, Cassia Regina Limonta Carvalho, Gabriel de Moraes Cunha Goncalves, Qijian Song, Sergio Augusto Morais Carbonell, Alisson Fernando Chiorato, Luciana Lasry Benchimol-Reis
Summary: This study identified the genetic control associated with lightness of seed coat color at harvest and tolerance to post-harvest seed coat darkening through a genome-wide association study. The results showed oligogenic inheritance for lightness of seed coat color with moderate broad-sense heritability. Only three QTL were significant for lightness of seed coat color, while six QTL were mapped for post-harvest seed coat darkening on different chromosomes, with Delta L* being a key parameter for this trait. Several candidate genes were identified, showing potential for accelerating the genetic breeding process.
Article
Biochemistry & Molecular Biology
Hongwei Jing, Lucia C. Strader
Summary: Auxin plays a crucial role in regulating plant development, with its effects on transcription mediated by the ARF family of transcription factors. ARFs tightly control specific auxin responses that are essential for proper plant growth and development. Recent research has highlighted the importance of regulated ARF protein accumulation and ARF nucleo-cytoplasmic partitioning in determining auxin transcriptional outputs. This review explores these recent findings and discusses the potential of regulated ARF accumulation in driving auxin responses in plants.
Article
Plant Sciences
Jeongho Choi, Hyojin Kim, Mi Chung Suh
Summary: Suberin, a complex polyester, plays a role in controlling the movement of water, ions, and gas in the seed coat outer integument. This study investigated the role of the plant hormone abscisic acid (ABA) in suberin layer formation during seed coat development. The results showed that ABA is important for regulating the permeability of the seed coat.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Kumari Arpita, Sandhya Sharma, Harsha Srivastava, Kuldeep Kumar, Muntazir Mushtaq, Palak Gupta, Rishu Jain, Kishor Gaikwad
Summary: Auxin Response Factors (ARF) are transcription factors that play a crucial role in auxin signaling and regulate various biological processes. This study focused on the systematic analysis of the ARF gene family in pigeonpea and identified key proteins controlling embryo and seed development. The analysis revealed the presence of 12 ARF genes in pigeonpea distributed across different chromosomes. These ARF proteins contain specific domains related to DNA binding, auxin response, and hormone signaling. The study also showed the expression patterns of different ARF genes in various tissues and their potential role in regulating embryo development and seed set in pigeonpea.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Kumari Arpita, Sandhya Sharma, Harsha Srivastava, Kuldeep Kumar, Muntazir Mushtaq, Palak Gupta, Rishu Jain, Kishor Gaikwad
Summary: In this study, a systematic analysis of the ARF gene family in pigeonpea was conducted, revealing the presence of 12 ARF genes involved in regulating seed and embryo development. These genes play significant roles in various biological processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Developmental Biology
Ron Eric Stein, Berit Helge Nauerth, Laura Binmoller, Luise Zuhl, Anna Loreth, Maximilian Reinert, David Ibberson, Anja Schmidt
Summary: Recent findings suggest that RNA helicases play a crucial role in plant reproductive development, with the RH17 gene identified as having previously unknown roles in repressing reproductive fate and seed development in the absence of fertilization. Mutations in RH17 can lead to the development of extra reproductive cell lineages and the occurrence of multiple embryos per seed, mimicking aspects of apomixis.
Article
Biochemistry & Molecular Biology
Shiv Mani Dubey, Soeun Han, Nathan Stutzman, Michael J. Prigge, Eva Medvecka, Matthieu Pierre Platre, Wolfgang Busch, Matyas Fendrych, Mark Estelle
Summary: The phytohormone auxin triggers root growth inhibition within seconds via a non-transcriptional pathway. The AFB1 protein plays a crucial role in this rapid response, and its N-terminal region, including the F-box domain and residues contributing to auxin binding, is essential for its specific function. A substitution of the N-terminal region of AFB1 disrupts its cytoplasmic localization and activity in rapid root growth inhibition. Moreover, AFB1 is involved in calcium influx triggered by auxin and negatively regulates lateral root formation and transcription of auxin-induced genes.
Article
Plant Sciences
Itay Cohen, Sara Zandalinas, Felix B. Fritschi, Soham Sengupta, Yosef Fichman, Rajeev K. Azad, Ron Mittler
Summary: This study investigated the physiological responses, seed production, and yield per plant of two soybean genotypes under conditions of water deficit and heat stress combination. The findings revealed that one genotype exhibited decreased seed production under the stress combination, possibly due to processes affecting flower development.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Tomasz Czechowski, Tony R. Larson, Theresa M. Catania, David Harvey, Cenxi Wei, Michel Essome, Geoffrey D. Brown, Ian A. Graham
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Plant Sciences
Theresa M. Catania, Caroline A. Branigan, Natalia Stawniak, Jennifer Hodson, David Harvey, Tony R. Larson, Tomasz Czechowski, Ian A. Graham
FRONTIERS IN PLANT SCIENCE
(2018)
Meeting Abstract
Biochemical Research Methods
L. Rossoni, J. P. Webb, V. Springthorpe, D. P. Minde, J. Bennett, D. J. Kelly, J. Green, K. S. Lilley, T. R. Larson, G. H. Thomas, G. Stephens
Article
Multidisciplinary Sciences
Fabian E. Vaistij, Thiago Barros-Galvao, Adama F. Cole, Alison D. Gilday, Zhesi He, Yi Li, David Harvey, Tony R. Larson, Ian A. Graham
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Romy R. Schmidt, Martin Fulda, Melanie V. Paul, Max Anders, Frederic Plum, Daniel A. Weits, Monika Kosmacz, Tony R. Larson, Ian A. Graham, Gerrit T. S. Beemster, Francesco Licausi, Peter Geigenberger, Jos H. Schippers, Joost T. van Dongen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Plant Sciences
Thiago Barros-Galvao, Anuja Dave, Adama Cole, David Harvey, Swen Langer, Tony R. Larson, Fabian E. Vaistij, Ian A. Graham
JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Microbiology
Joseph Webb, Vicki Springthorpe, Luca Rossoni, David-Paul Minde, Swen Langer, Heather Walker, Amias Alstrom-Moore, Tony Larson, Kathryn Lilley, Graham Eastham, Gill Stephens, Gavin H. Thomas, David J. Kelly, Jeffrey Green
Article
Plant Sciences
Tomasz Czechowski, Mauro A. Rinaldi, Mufuliat Toyin Famodimu, Maria Van Veelen, Tony R. Larson, Thilo Winzer, Deborah A. Rathbone, David Harvey, Paul Horrocks, Ian A. Graham
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Tiago R. Ferreira, Adam A. Dowle, Ewan Parry, Eliza V. C. Alves-Ferreira, Karen Hogg, Foteini Kolokousi, Tony R. Larson, Michael J. Plevin, Angela K. Cruz, Pegine B. Walrad
NUCLEIC ACIDS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Tomasz Czechowski, Edith Forestier, Sandesh H. Swamidatta, Alison D. Gilday, Amy Cording, Tony R. Larson, David Harvey, Yi Li, Zhesi He, Andrew J. King, Geoffrey D. Brown, Ian A. Graham
Summary: The majority of macro-and polycyclic Euphorbiaceae diterpenoids are derived from the common C20 precursor casbene, but the biosynthetic pathways for some of these diterpenoids are not yet clear. Using a metabolomics-guided transcriptomic approach, two casbene-derived diterpenoid gene clusters were discovered, with one gene identified as playing a crucial role in converting casbene to lathyrane diterpenoids.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Correction
Multidisciplinary Sciences
Theresa Catania, Yi Li, Thilo Winzer, David Harvey, Fergus Meade, Anna Caridi, Andrew Leech, Tony R. Larson, Zemin Ning, Jiyang Chang, Yves Van de Peer, Ian A. Graham
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Theresa Catania, Yi Li, Thilo Winzer, David Harvey, Fergus Meade, Anna Caridi, Andrew Leech, Tony R. Larson, Zemin Ning, Jiyang Chang, Yves Van de Peer, Ian A. Graham
Summary: The STORR gene fusion event is essential for the evolution of benzylisoquinoline alkaloids in opium poppy, and it occurred only in specific species within the Papaver genus.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Andrew S. Mason, Claire L. Varley, Olivia M. Foody, Xiang Li, Katie Skinner, Dawn Walker, Tony R. Larson, Daisuke Wakamatsu, Simon C. Baker, Jennifer Southgate
Summary: Urothelium is a specialized epithelium that lines the lower urinary tract and plays a crucial role in maintaining barrier function. This study explored the role of glycerophospholipids in urothelial barrier function and demonstrated the importance of lipid mediators in tissue homeostasis. Transcriptomic analysis revealed metabolic changes during urothelial differentiation, and knocking down the LPCAT4 gene significantly affected cell proliferation and barrier function recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Jordan Tzvetkov, Louise A. Stephen, Scott Dillon, Jose Luis Millan, Anke J. Roelofs, Cosimo De Bari, Colin Farquharson, Tony Larson, Paul Genever
Summary: Lipids play a crucial role in regulating the development and maintenance of the skeleton. This study investigated the lipid atlas of healthy mouse knee and revealed the effects of PHOSPHO1 ablation on the growth plate lipidome. The findings highlight the essential role of PHOSPHO1-mediated membrane phospholipid metabolism in lipid and bone homeostasis.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Plant Sciences
Samuel H. Taylor, Emmanuel Gonzalez-Escobar, Rhiannon Page, Martin A. J. Parry, Stephen P. Long, Elizabete Carmo-Silva
Summary: Cowpea is the major source of vegetable protein for rural populations in sub-Saharan Africa. The speed of photosynthetic adjustment to shade strongly affects daily carbon gain. Direct biochemical measurements showed a faster rate of Rubisco deactivation in cowpea than previously estimated. This suggests that slowing Rubisco deactivation during shade is an unexploited opportunity for improving crop productivity.
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)