期刊
PLANT BIOTECHNOLOGY
卷 29, 期 3, 页码 319-322出版社
JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY
DOI: 10.5511/plantbiotechnology.12.0322a
关键词
5 '-UTR; Arabidopsis thaliana arabinogalactan-protein 21; horseradish peroxidase
资金
- Development of Fundamental Technologies for the Production of High-Value Materials Using Transgenic Plants project
For higher expression of a foreign gene in plant cells, it is important to optimize nucleotide sequences corresponding to 5'-untranslated region (5'-UTR), because it usually has great impacts on the expression of the gene mainly at the translational level. In this study, with an aim to find useful 5'-UTRs, thirty nine 5'-UTRs derived from Arabidopsis thaliana genes were tested by transient expression of firefly luciferase (Fluc), and that of A. thaliana arabinogalactan-protein 21 (AtAGP21) gene was selected for further analyses. Its activity was either equaling or surpassing that of known translational enhancer, A. thaliana alchol dehydrogenase (AtADH) 5'-UTR in dicotyledons, and was further improved by the optimizing sequence context of the initiating codon (-3 to -1 of AUG). Finally, we also found that the modified AtAGP21 5'-UTR was useful in recombinant expression of horseradish peroxidase (HRP) in tobacco cultured cells, and the yield was as much as 23 mgl(-1) culture medium in seven days.
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