Review
Plant Sciences
Claudia E. Osorio, Bradley J. Till
Summary: This review summarizes the current status of research on quinolizidine alkaloids in lupin breeding, including the utilization of natural mutations and molecular markers for identifying candidate genes. Future research directions, such as using differentially expressed genes on the pauper locus for genome editing, are also provided.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Gagan Garg, Lars G. Kamphuis, Philipp E. Bayer, Parwinder Kaur, Olga Dudchenko, Candy M. Taylor, Karen M. Frick, Rhonda C. Foley, Ling-Ling Gao, Erez Lieberman Aiden, David Edwards, Karam B. Singh
Summary: Narrow-leafed lupin is a high-protein grain suitable for sustainable farming. This study provides a reference genome and pan-genome assembly for narrow-leafed lupin, compares it with the genome of its close relative white lupin, and identifies key alkaloid regulatory genes and the deficiency of disease resistance genes in the narrow-leafed lupin genome. These findings offer new opportunities for lupin crop improvement.
Article
Biochemistry & Molecular Biology
Katarzyna Czepiel, Pawel Krajewski, Paulina Wilczura, Patrycja Bielecka, Wojciech swiecicki, Magdalena Kroc
Summary: The study revealed the significant upregulation of RAP2-7 and other QA-related genes in various organs of bitter lupin, supporting the important role of RAP2-7 in regulating QA accumulation. Other regulatory steps triggered by RAP2-7 may be involved in QA accumulation, particularly in leaves. There was no connection found between QA synthesis and anthracnose resistance in lupin plants infected with Colletotrichum lupini, unlike the role of polyamines in plant-pathogen interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Moussa Bouray, James Laing Moir, Niklas Jussi Lehto, Leo Murtagh Condron, Driss Touhami, Christina Hummel
Summary: The study revealed that increasing the soil pH to 6.3 using lime negatively affects phosphorus acquisition by blue lupin, leading to a reduction in organic anion exudation, fine root length, and acid phosphomonoesterase activity in the rhizosphere.
Article
Agronomy
Wiktor Skrzypkowski, Renata Galek, Adela Adamus, Agnieszka Kielkowska
Summary: This study analyzed pollen development and staining in Vicia faba L. and Lupinus angustifolius L., two species belonging to the Fabaceae family. Various dyes were tested to assess the viability of microspores and pollen grains. The study found that different dyes could distinguish viable and nonviable microspores and pollen grains. In vitro germination tests also showed the impact of different growth media on pollen germination.
Article
Plant Sciences
Ida K. L. Andersen, Lars O. Dragsted, Jim Rasmussen, Inge S. Fomsgaard
Summary: Intercropping barley affects the metabolome of lupin, leading to increased levels of flavonoids and decreased levels of tryptophan. These flavonoids may play a role in regulating lupin's nitrogen fixation activity.
JOURNAL OF PLANT INTERACTIONS
(2023)
Article
Agriculture, Multidisciplinary
Laura Roman, Emmanouil Tsochatzis, Kubra Tarin, Eje M. Rondahl, Carl-Otto Ottosen, Milena Corredig
Summary: This study evaluated 22 different blue lupin genotypes for protein concentration and antinutritional alkaloid content. The results showed that blue lupin has high protein concentration and low phytic acid content, and certain varieties also have low alkaloid content. Lupin proteins are rich in leucine and lysine, but have low methionine concentration. This study demonstrated the importance of considering both agronomic yields and nutritional phenotypes when selecting lupin genotypes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Nouha Ferchichi, Wael Toukabri, Urska Vrhovsek, Issam Nouairi, Andrea Angeli, Domenico Masuero, Ridha Mhamdi, Darine Trabelsi
Summary: Lupine seed shows significant differences in nutritional composition between species and geographical ecotypes, with white lupine having the highest protein and oleic acid content, while yellow lupine ecotypes vary greatly in oleic and linoleic acid content. The lipid and phenolic profiles of lupine seeds are highly influenced by ecological factors, with some ecotypes showing unique compounds and antioxidant activities.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2021)
Article
Chemistry, Applied
Sofie Schryvers, Chinaza Arinzechukwu, Bram Miserez, Mia Eeckhout, Liesbeth Jacxsens
Summary: This study assessed the reduction of quinolizidine alkaloids (QAs) during the processing of Lupinus albus seeds and lupin-based foods. The results showed that the decrease in QA content depends on the specific processing method, and QAs are heat stable while depletion occurs due to leaching in cooking water.
Review
Chemistry, Applied
Ana Lemus-Conejo, Fernando Rivero-Pino, Sergio Montserrat-de la Paz, Maria C. Millan-Linares
Summary: Narrow-leafed lupin is a nutrient-rich plant, widely used for human food and animal fodder. There is a growing interest in plant protein-derived products due to their ecological benefits and lower production costs. This review summarizes the chemical components and health benefits of narrow-leafed lupin, focusing on its protein fraction and biological properties. The seed and protein by-products of narrow-leafed lupin can be valuable sources for high value-added compounds in diverse food products, maximizing economic value.
Article
Agriculture, Multidisciplinary
Jaroslaw Czubinsk, Erika Lattova, Zbynek Zdrahal, Richard Strasser
Summary: This study provides a comprehensive characterization of ?-conglutin glycosylation, including the identification of the N-glycan-bearing site, the composition of glycan-building saccharides, and the effect of oligosaccharide removal on structural and thermal stability. The results indicate the presence of glycans belonging to different classes attached to the Asn98 residue. The removal of the oligosaccharide significantly affects the secondary structure composition, leading to a disturbance in the oligomerization process.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Cristina Poyatos, Sandra Sacristan-Bajo, Pablo Tabares, Samuel Prieto-Benitez, Maria Luisa Rubio Teso, Elena Torres, Javier Morente-Lopez, Carlos Lara-Romero, Jose Maria Iriondo, Alfredo Garcia-Fernandez
Summary: The study assessed the phenotypic variation of Lupinus angustifolius within and between populations, finding that southern populations showed traits adapted to high temperatures and drought. These populations may be more vulnerable to climate change.
Article
Food Science & Technology
Elif Yaver, Nermin Bilgicli
Summary: This study aimed to develop a new method for debittering lupin. The results showed that ultrasound-accelerated debittering method had shorter processing time and lower alkaloid content in lupin, while also improving storage stability.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Plant Sciences
Moussa Bouray, James Laing Moir, Leo Murtagh Condron, Niklas Jussi Lehto
Summary: The study found that lime application significantly influences the biochemical processes and dynamics of phosphorus (P) in the rhizosphere of lupins. Russell lupin utilizes less P than blue lupin and is unresponsive to liming.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2021)
Article
Agriculture, Multidisciplinary
Anna Maria Engel, Fenja Klevenhusen, Jan-Louis Moenning, Jorge Numata, Carola Fischer-Tenhagen, Benjamin Sachse, Bernd Schaefer, Hildburg Fry, Oliver Kappenstein, Robert Pieper
Summary: This study is the first to quantitatively evaluate the transfer of quinolizidine alkaloids (QAs) from blue sweet lupin (BSL) to cow's milk using experimental and modeling methods. The results indicate a potential health concern from QAs in milk.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Tsubasa Shoji, Naoyuki Umemoto, Kazuki Saito
Summary: Plant domestication and improvement have led to mutational changes in transcriptional regulators of defense metabolism. Specialized compounds like alkaloids and terpenoids play essential roles in plant defense against herbivores and microbes. Transcription factors coordinately regulate defense metabolism genes by recognizing cis-regulatory elements in the promoter regions of target genes.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Plant Sciences
Atsushi Fukushima, Mikiko Takahashi, Hideki Nagasaki, Yusuke Aono, Makoto Kobayashi, Miyako Kusano, Kazuki Saito, Norio Kobayashi, Masanori Arita
Summary: The advancement of metabolomics has enabled rapid detection and quantification of cellular metabolites. However, shortcomings in data sharing still exist. To address these issues, RIKEN developed the RIKEN PMM, a database that stores plant metabolomic data and experimental metadata.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Kanokporn Damrongrak, Kiattiphant Kloysawat, Somnuk Bunsupa, Krisada Sakchasri, Amaraporn Wongrakpanich, Vincenzo Taresco, Valentina Cuzzucoli C. Crucitti, Martin C. Garnett, Jiraphong Suksiriworapong
Summary: The study demonstrated the fabrication of poly(glycerol adipate) nanoparticles decorated with folic acid and triphenylphosphonium and their potential for delivering acetogenin-enriched leaf extract to ovarian cancer cells. The nanoparticles exhibited small size, narrow size distribution, high negative surface charge, and a biphasic sustained release of the extract. The FOL/TPP-decorated nanoparticles showed higher drug loading, enhanced toxicity to cancer cells, and improved cellular uptake compared to FOL-decorated nanoparticles. The FOL/TPP-NPs also demonstrated better stability, uptake efficiency, and targeting ability to mitochondria.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Plant Sciences
Ryota Akiyama, Bunta Watanabe, Junpei Kato, Masaru Nakayasu, Hyoung Jae Lee, Naoyuki Umemoto, Toshiya Muranaka, Kazuki Saito, Yukihiro Sugimoto, Masaharu Mizutani
Summary: The metabolic detoxification of alpha-tomatine is an important trait in cultivated tomato, and two enzymes play a key role in this process. The functional divergence of these enzymes is a result of gene duplication and neofunctionalization, contributing to the structural diversity of steroidal glycoalkaloids in tomatoes.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Kanade Tatsumi, Takuji Ichino, Natsumi Isaka, Akifumi Sugiyama, Eiko Moriyoshi, Yozo Okazaki, Yasuhiro Higashi, Masataka Kajikawa, Yoshinori Tsuji, Hideya Fukuzawa, Kiminori Toyooka, Mayuko Sato, Ikuyo Ichi, Koichiro Shimomura, Hiroyuki Ohta, Kazuki Saito, Kazufumi Yazaki
Summary: Plants produce a variety of lipophilic metabolites, some of which are secreted by cells. Triacylglycerol acts as a matrix lipid to facilitate the secretion of these lipophilic compounds.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Tsubasa Shoji, Kazuki Saito
Summary: A group of specialized metabolites called steroidal glycoalkaloids (SGAs) are produced in Solanum species and their biosynthesis is regulated by the transcription factor JASMONATA-RESPONSIVE ETHYLENE RESPONSE FACTOR 4 (JRE4). In this study, the downregulation of a cluster of genes encoding nitrate transporter 1/peptide transporter family (NPF) members was observed in a low-SGA tomato mutant with a loss-of-function mutation in JRE4. The NPF transporters, which belong to a large family of plant membrane transporters, are induced by the defense-related phytohormone jasmonate and are potentially involved in the SGA pathway.
PLANT BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nantana Nuchtavorn, Jiraporn Leanpolchareanchai, Satsawat Visansirikul, Somnuk Bunsupa
Summary: In this study, novel magnetic nanoparticles Fe3O4@MIPs and filter paper@MIPs were synthesized by sol-gel polymerization for selective extraction of charantin. Compared to traditional liquid-liquid extraction, these methods exhibited excellent extraction efficiency and selectivity, and were rapid, inexpensive, simple, reproducible, and environmentally friendly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Correction
Chemistry, Medicinal
Naoko Yoshimoto, Takashi Asano, Ayuna Kisanuki, Chihiro Kanno, Machiko Asanuma, Mami Yamazaki, Isao Fujii, Kazuki Saito
JOURNAL OF NATURAL MEDICINES
(2023)
Article
Pharmacology & Pharmacy
Jiraphong Suksiriworapong, Nutthachai Pongprasert, Somnuk Bunsupa, Vincenzo Taresco, Valentina Cuzzucoli Crucitti, Thitapa Janurai, Pornpoj Phruttiwanichakun, Krisada Sakchaisri, Amaraporn Wongrakpanich
Summary: This study aimed to enhance the anticancer effect of Cordyceps militaris herbal extract (CME) on breast cancer cells by using hyaluronic acid (HYA) surface-decorated lipid polymer hybrid nanoparticles (LPNPs), and evaluate the feasibility of using a synthesized poly(glycerol adipate) (PGA) polymer for LPNP preparation. The results showed that the synthesized polymers could successfully encapsulate CME in LPNPs. The HYA-decorated PGA-based LPNPs significantly improved the anticancer effect of CME against breast cancer cells by enhancing cellular uptake through CD44 receptor-mediated endocytosis.
Article
Plant Sciences
Soichiro Honda, Yumiko Yamazaki, Takumi Mukada, Weiguo Cheng, Masaru Chuba, Yozo Okazaki, Kazuki Saito, Akira Oikawa, Hayato Maruyama, Jun Wasaki, Tadao Wagatsuma, Keitaro Tawaraya
Summary: Plants have evolved mechanisms to tolerate low P, including changing their membrane lipid composition. This study investigated the membrane lipid remodeling among different rice cultivars under P deficiency. The levels of phospholipids were lower in -P plants compared to +P plants, while the levels of non-phospholipids were higher in -P plants. Decomposition of phospholipids in roots correlated with low P tolerance. These findings suggest that rice cultivars remodel membrane lipids under P deficiency, contributing to their low P tolerance.
Review
Plant Sciences
Tsubasa Shoji, Takashi Hashimoto, Kazuki Saito
Summary: Tobacco is a widely cultivated crop, but its highly addictive nature poses a significant risk to human health. Recent research in molecular and genomics has led to advancements in understanding the metabolic and regulatory genes involved in nicotine biosynthesis, allowing the development of tobacco plants with ultra-low nicotine levels through mutational breeding, genetic engineering, and genome editing.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Pharmacology & Pharmacy
Jiraphong Suksiriworapong, Chittin Achayawat, Phutthikom Juangrattanakamjorn, Vincenzo Taresco, Valentina Cuzzucoli Crucitti, Krisada Sakchaisri, Somnuk Bunsupa
Summary: This study aimed to fabricate new variations of glycerol-based polyesters by grafting poly(glycerol adipate) (PGA) with hydrophobic bioactive moieties, tocopherol (TOC), and cholesterol (CHO). Their effects on nanoparticle (NP) formation, drug release, and cellular responses in cancer and normal cells were evaluated. The results showed that both PGA-TOC NPs and PGA-CHO NPs could be used as delivery systems for anticancer drugs, with different characteristics and effects on cancer and normal cells.
Article
Multidisciplinary Sciences
Tsubasa Shoji, Satoko Sugawara, Tetsuya Mori, Makoto Kobayashi, Miyako Kusano, Kazuki Saito
Summary: This study investigates the regulatory function of the transcription factor PhERF1 in petunia, which controls the synthesis of bioactive alkaloids and terpenoids. The study demonstrates that PhERF1 can induce the production of specialized steroids by transcriptional reprogramming. This highlights the potential of transcriptional regulators in producing specialized products.
Review
Biochemistry & Molecular Biology
Chaiwat Aneklaphakij, Phatthilakorn Chamnanpuen, Somnuk Bunsupa, Veena Satitpatipan
Summary: Stilbenoids, well-known phytoalexins, have various bioactivities and are widely used in cosmetic products. However, the supply of stilbenoids from plants is limited and traditional organic solvent extraction methods are harmful to the environment and human health. Therefore, researchers are focusing on green technologies to achieve sustainable stilbenoids production.