Article
Biotechnology & Applied Microbiology
Cintia Lucia Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah McHale, Tom Elmo Clemente, Ana Paula Alonso, Chi Zhang
Summary: This study evaluated the metabolic and transcriptional responses to the expression of AtWRI1 and AtDGAT1 in soybean seeds. The results showed that the expression of these genes did not increase the total fatty acid content, but led to changes in the oil and carbohydrate composition. Transcriptomic analysis revealed down-regulation of oil body packaging protein genes and strong induction of lipase and FA biosynthesis inhibitor genes.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Cell Biology
Pina Marotta, Federica Salatiello, Luca Ambrosino, Federica Berruto, Maria Luisa Chiusano, Annamaria Locascio
Summary: The AP-1 transcription factor family is composed of Jun, Fos, Atf3, Atf2/7, and Maf in the tunicate Ciona robusta, where they are mainly expressed in mesenchyme cells during embryonic development, with Fos also showing expression in notochord and neural cells. By ectopic expression in transgenic embryos, the study confirmed functional conservation of the AP-1 complex between Ciona and vertebrates in controlling cell movements and structural organization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Jie Lin, Ivan Monsalvo, Melissa Ly, Md Asraful Jahan, Dasol Wi, Izabella Martirosyan, Nik Kovinich
Summary: Glyceollins are important phytoalexins in soybean and have antimicrobial, anticancer, and neuroprotective activities. Co-overexpression of GmMYB29A2 and GmNAC42-1 is insufficient to activate glyceollin biosynthesis. GmMYB29A2 can upregulate most of the biosynthesis genes, while GmNAC42-1 only upregulates them in the presence of an elicitor.
Article
Agriculture, Multidisciplinary
Andre Luis Hartmann Caranhato, Jessika Angelotti-Mendonca, Liliane Marcia Mertz-Henning, Silvana Regina Rockenbach Marin, Carlos Lasaro Pereira de Melo, Jose Salvador Simoneti Foloni, Norman Neumaier, Jose Renato Boucas Farias, Alexandre Lima Nepomuceno
Summary: The objective of this study was to investigate the effect of introgressing the AtAREB1 gene into elite soybean germplasms on their tolerance to water deficit. The results showed that soybean genotypes containing the AtAREB1 gene exhibited improved physiological performance and altered expression of drought-responsive genes under drought stress, regardless of the genetic background in which the gene was introduced.
PESQUISA AGROPECUARIA BRASILEIRA
(2022)
Article
Plant Sciences
Ling Jiang, Xiewang Gao, Xiaofeng Yang, Shan Huang, Wenjun Tang, Xiaohong Li, Shumei Ma, Mu Xiao
Summary: Di19 is a family of transcription factors involved in stress responses, growth, and development, with GmDi19-15 identified as a novel transcription factor specifically expressed in roots. Its potential role as a transcription regulator was hinted by transactivation activity in yeast cells, and its ectopic expression in Arabidopsis resulted in compromised drought tolerance. Our study expanded the GmDi19 family with 8 new members and proposed GmDi19-15 as a target for tissue-specific genetic modification and enhancing stress tolerance traits.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Chengfeng Yang, Yanzhong Huang, Peiyun Lv, Augustine Antwi-Boasiako, Naheeda Begum, Tuanjie Zhao, Jinming Zhao
Summary: NAC transcription factor GmNAC12 in soybeans has been found to be involved in regulating stress tolerance, particularly under drought conditions. Overexpression of GmNAC12 increased survival rates, while knockout of GmNAC12 decreased survival rates, under drought stress. Additionally, GmNAC12 protein localizes in the nucleus and interacts with proteins related to metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
Lin Yue, Xinxin Pei, Fanjiang Kong, Lin Zhao, Xiaoya Lin
Summary: Soybean (Glycine max) is a key crop for protein and oil production. The bZIP transcription factors in soybean play important roles in various regulatory pathways, such as yield improvement, stress response, environmental signaling, and carbon-nitrogen balance. This review provides a comprehensive understanding of the soybean bZIP family, including its members and classification, and offers valuable insights for future research on bZIP transcription factors and genetic resources for soybean breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ruyi Xiao, Yan Sun, Shu Yang, Yixiao Yang, Donghao Wang, Zhezhi Wang, Wen Zhou
Summary: Basic leucine zipper (bZIP) transcription factors are important for plant growth, development, and stress responses. This study identified and analyzed the bZIP gene family in Hypericum perforatum, a popular herbal medicine used for depression treatment. The results revealed the functional role of HpbZIP69 and its involvement in drought tolerance in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Huan Han, Caini Wang, Xiaoyan Yang, Lina Wang, Jiabao Ye, Feng Xu, Yongling Liao, Weiwei Zhang
Summary: This study provides an overview of the structure, classification, regulatory mechanisms, and biological functions of basic leucine zipper transcription factors (bZIPs) and their molecular mechanisms in the biosynthesis of various secondary metabolites. bZIPs are highly conserved transcription factors in eukaryotic organisms and play crucial roles in plant growth and development, stress response, and secondary metabolism. The paper elaborates on the structure, classification, biological function, and regulatory mechanisms of bZIPs, as well as their molecular mechanisms in regulating the biosynthesis of flavonoids, terpenoids, alkaloids, phenolic acids, and lignin. This review is significant for understanding the molecular mechanisms of bZIPs in secondary metabolite synthesis and plant molecular breeding.
Article
Biochemistry & Molecular Biology
Hyuk Sung Yoon, Kaien Fujino, Shenkui Liu, Tetsuo Takano, Daisuke Tsugama
Summary: The study shows that Arabidopsis PP2A B''-family subunits interact with PP2A A (scaffold) subunits and can mediate VIP1 dephosphorylation. This provides important insights into the molecular mechanism of VIP1 dephosphorylation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Zening Zhang, Pengyan Qu, Siyi Hao, Ruide Li, Yongyan Zhang, Qi Zhao, Pengfei Wen, Chunzhen Cheng
Summary: In this study, we characterized and studied the CHS gene family in highbush blueberry. We identified 22 CHS genes and classified them into four subfamilies. Gene expression analysis showed that VcCHSs in subfamily I were highly expressed in late ripening stages of the fruit. By transient overexpression, we found that three VcCHSs could enhance anthocyanin accumulation and up-regulate the expression of flavonoid biosynthetic genes. Promoter analysis revealed multiple cis-acting elements and transcription factor binding sites in VcCHSs. Our study provides insights into the characteristics and functions of blueberry CHSs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Qi Wang, Cun Guo, Zhiyuan Li, Jinhao Sun, Dong Wang, Liangtao Xu, Xiaoxu Li, Yongfeng Guo
Summary: In this study, 49 bZIP transcription factor-encoding genes were identified and analyzed on the potato genome. These genes were found to be located on 12 chromosomes of the potato genome, displaying functional diversity. Syntenic analysis revealed gene duplication events that contribute to the expansion of the bZIP gene family in potato.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Shuaike Niu, Xiangyang Gu, Qian Zhang, Xuemin Tian, Zhan Chen, Jingru Liu, Xiaoju Wei, Chengxiang Yan, Ziwen Liu, Xiaoji Wang, Zhengge Zhu
Summary: In this study, a gene called VvANN1 was characterized, which was found to play a positive role in the response to drought stress. VvANN1 was induced by osmotic stress and enhanced tolerance to osmotic and drought stress in Arabidopsis thaliana. It was also discovered that VvbZIP45 can regulate VvANN1 expression by binding to its promoter region under drought stress. These findings are important for understanding the mechanisms of grapevine adaptation to drought.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Lin-Tao Huang, Chang-Yan Liu, Li Li, Xue-Song Han, Hong-Wei Chen, Chun-Hai Jiao, Ai-Hua Sha
Summary: In this study, 18 members of the faba bean bZIP transcription factor family were identified and their involvement in drought stress response was investigated. Among them, VfbZIP5 was found to enhance drought tolerance through protein-protein interactions. These findings highlight the importance of bZIP transcription factors in drought response and provide potential targets for improvement of drought tolerance in faba bean.
Article
Chemistry, Applied
Li Zheng, ZhongJiang Wang, Yang Kong, ZhaoLei Ma, ChangLing Wu, Joe M. Regenstein, Fei Teng, Yang Li
Summary: Twenty different cold gels were made using transglutaminase enzyme and soy protein isolate (SPI) powders from various manufacturers to study their potential as an ingredient in Chiba tofu. The study found that SPI contributes to Chiba tofu's high hardness, springiness, and quality, with key characteristics including sulfur-containing amino acid content >2.83%, beta-sheets content >52.1%, and an 11S:7S ratio > 1.88. Additionally, water mobility in the best sample was confirmed to be higher, leaving more water in the gel network.
FOOD HYDROCOLLOIDS
(2021)