Review
Chemistry, Analytical
Ying Wen, Ning Jing, Fangjun Huo, Caixia Yin
Summary: Fluorescent probes and fluorescence microscopy are indispensable tools in biomedical research, enabling the exploration of cellular chemical factors and their physiological functions and pathological changes. Intracellular pH value is a critical factor, while different organelles possess unique acid-base homeostasis involved in important physiological processes.
Article
Spectroscopy
Yutao Jia, Yanting Shen, Yanyan Zhu, Jing Wang
Summary: This study reports a novel covalent organic framework as a pH-responsive fluorescent probe for lysosomal pH imaging in cells. The probe exhibits good pH-responsive properties and can effectively monitor pH fluctuations and changes in lysosomes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Chemistry, Multidisciplinary
Zhen Li, Caixia Wang, Meng Zhang, Songjiao Li, Zhiqiang Mao, Zhihong Liu
Summary: The brain, as the most pivotal organ of the human body, is susceptible to various disorders and trauma. Despite considerable efforts in treatment, brain diseases remain a leading cause of death and disability globally, making early diagnosis crucial for prevention and treatment. Luminescent probes, particularly activatable ones, are promising tools for brain disease diagnosis, offering real-time semi-quantification of biomarkers and investigation of pathological mechanisms at the molecular level.
Review
Chemistry, Multidisciplinary
Jiawei Wang, Yibiao Jin, Mingdang Li, Shujuan Liu, Kenneth Kam-Wing Lo, Qiang Zhao
Summary: Enzyme activity detection is crucial for early diagnosis of diseases and drug screening. Time-resolved luminescence assays, with their high accuracy and signal-to-noise ratios, have been widely used. Responsive probes based on metal complexes and metal-free organic compounds have been developed for time-resolved bioimaging and biosensing due to their long luminescence lifetimes, high quantum yields, and photostability.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Review
Chemistry, Analytical
Zixuan Zhan, Yongcheng Dai, Qiuyan Li, Yi Lv
Summary: Small molecules-based bioluminescence and chemiluminescence probes have emerged as powerful tools for biomarkers imaging without requiring excitation light source, showing intriguing properties and fast development. However, probes for reactive species have not been reviewed comprehensively in the past decade. Recent research indicates the significant potential of these probes in the field of biomedical imaging.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Franka H. van der Linden, Eike K. Mahlandt, Janine J. G. Arts, Joep Beumer, Jens Puschhof, Saskia M. A. de Man, Anna O. Chertkova, Bas Ponsioen, Hans Clevers, Jaap D. van Buul, Marten Postma, Theodorus W. J. Gadella, Joachim Goedhart
Summary: The study introduces a new genetically encoded calcium indicator, Tq-Ca-FLITS, which enables reliable measurement of intracellular calcium concentrations through fluorescence lifetime imaging, addressing the quantification issues associated with existing GECIs.
NATURE COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
You Wu, Chengyan Ge, Ying Zhang, Yalong Wang, Deteng Zhang
Summary: Fluorescent probes based on the intramolecular charge transfer mechanism have been widely studied for cell detection due to their non-invasiveness, fast response, high sensitivity, and low damage tolerance. Monitoring the intracellular pH and various biological species is crucial for understanding the status of organisms and preventing, diagnosing, and treating diseases. This review highlights the recent development of ICT probes for the detection of intracellular pH, gases, metal ions, and anions, and discusses their limitations and translational potential.
FRONTIERS IN CHEMISTRY
(2023)
Article
Chemistry, Analytical
Fan Liu, Wusen Zhou, Xingjun Li, Zhuo Li, Shan Lu, Xiaoying Shang, Chong Tan, Ping Hu, Zhuo Chen, Xueyuan Chen
Summary: In this study, hybrid nanocomposites NaEuF4@TCPP were constructed, which can selectively quench Fe3+ ions and be applied for sensitive detection of Fe3+ ions in living cells. The lipo-coated NaEuF4@TCPP probes showed good biocompatibility and stability, making them suitable for real-time monitoring of Fe3+ ions in living cells.
Review
Environmental Sciences
Sandip Ghosh, Jui-Yang Lai
Summary: Intracellular pH (pHi) measurement is crucial for understanding physiological activities and diagnosing diseases. Non-invasive nanoprobes based on nanotechnology have been developed for accurate and repeatable pHi sensing using Raman spectroscopy and fluorescence imaging. However, there are still limitations and challenges that need to be addressed. Future advancements can be made by exploring alternative nanomaterials and technologies to enhance sensitivity and reliability of pHi measurement.
ENVIRONMENTAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Pankaj E. Hande, Yogesh G. Shelke, Anindya Datta, Santosh J. Gharpure
Summary: Intracellular pH plays a crucial role in biological and pathological processes. Small-molecule based pH probes, known for their ease of preparation, high sensitivity, and quick response, are considered the most effective for pH sensing. This review highlights recent advancements in the development of such probes, categorizing them based on the fluorophore used and discussing their analytical performance.
Article
Spectroscopy
Xiaohui Wang, Yuanxiangyi Feng, Jinhua Liu, Kun Cheng, Yuanan Liu, Wei Yang, Hongxin Zhang, Hongshang Peng
Summary: The dual-photon pH nanosensor based on FITC-PFO NPs exhibits high sensitivity, excellent stability, and low cytotoxicity. It shows efficient pH-sensitive response under two-photon excitation and can be applied for ratiometric imaging of intracellular pH fluctuations.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Patrick K. Capon, Jiawen Li, Aimee J. Horsfall, Suliman Yagoub, Erik P. Schartner, Asma Khalid, Rodney W. Kirk, Malcolm S. Purdey, Kylie R. Dunning, Robert A. McLaughlin, Andrew D. Abell
Summary: A new fiber functionalization architecture enables simultaneous imaging and sensing, providing significant insights into complex biological systems.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Sun Hyeok Lee, Chul Soon Park, Kyung Kwan Lee, Tae-Hee Han, Hyun Seung Ban, Chang-Soo Lee
Summary: In this study, novel near-infrared probes were synthesized for fluorescence imaging of NTR activity. NIR-HCy-NO2 1 exhibited superior performance compared to the other two probes in terms of catalytic efficiency and limit of detection. Furthermore, NIR-HCy-NO2 1 was found to react with type I mitochondrial NTR in live cell imaging. NIR-HCy-NO2 demonstrated great potential for various NTR-related applications, including NTR activity imaging in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Hui Chen, Caixia Luo, Shangtao Zhang
Summary: Exosomes have been highly studied for their potential as cancer diagnostic targets and therapeutic agents. A new method using SERS technology has been demonstrated for imaging exosomes intracellularly and sensing pH simultaneously. This technique allows for tracking exosomal distribution within cells and quantitative pH sensing, providing insights into interactions between exosomes and cells.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Organic
Nadege Hamon, Lucile Bridou, Margaux Roux, Olivier Maury, Raphael Tripier, Maryline Beyler
Summary: Previously, Lanthanide Luminescent Bioprobes (LLBs) based on pyclen-bearing pi-extended picolinate antennas were synthesized and shown to have suitable optical properties for biphotonic microscopy. This study aims to develop a strategy to design bifunctional analogues of the studied LLBs by adding a reactive chemical group for coupling to biological vectors in deep in vivo targeted two-photon bioimaging. A synthetic scheme was elaborated to introduce a primary amine on the para position of the macrocyclic pyridine unit. Photophysical and bioimaging studies demonstrate that the addition of the reactive function does not affect the luminescent properties of the LLBs, which opens up possibilities for further applications.
JOURNAL OF ORGANIC CHEMISTRY
(2023)