Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Chang-Hun Choi, Eiseul Kim, Seung-Min Yang, Da-Som Kim, Seung-Man Suh, Ga-Young Lee, Hae-Yeong Kim
Summary: This study evaluated the applicability of a ddPCR assay for detecting lactic acid bacteria and compared its performance characteristics to those of a qPCR. The results showed that ddPCR is more sensitive than qPCR, but it has limitations in quantifying high bacterial concentrations.
Article
Pathology
Shu Jin Tan, Todd Matthew Pryce, Erin Judith Haygarth, Peter Alan Boan
Summary: The study demonstrated the utility of a Pneumocystis jirovecii quantitative polymerase chain reaction (qPCR) in distinguishing disease from colonization, with varying sensitivity and specificity in HIV and non-HIV patients. Standardization of methods and definitions is necessary for improving generalizability of results.
Article
Microbiology
Bingqian Zhou, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang, Cheng Du
Summary: A new qPCR method was developed to detect T. haneyi infection in horses, showing high specificity and sensitivity. Its accuracy was further validated by comparison with the traditional nested PCR method.
Article
Horticulture
Chenjie Zhang, Chengwei Song, Linfeng Chen, Huili Ma, Yabing Zhang, Dalong Guo, Lili Guo, Xiaogai Hou
Summary: The aim of this study was to identify suitable reference genes for normalizing the expression levels of miRNA in different developmental stages and tissues. Five algorithms were used for stability analysis, and the results identified mtr-MIR160b-p3 and gma-miR394a-5p as the most stable miRNAs during bud development in early-flowering tree peony 'Feng dan', while PC-5p-19095 was the most stable during bud development in late-flowering tree peony 'Lian he'. Additionally, mtr-miR159a was found to be the most stable miRNA during flower development stages of different tree peony varieties.
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Immunology
Xiaolin Jiang, Yong Jiang, Feng Ye
Summary: This study used real-time quantitative PCR to detect Mucorales and Aspergillus in formalin-fixed, paraffin-embedded samples. The combination of Mucorales RQ-PCR and Aspergillus RQ-PCR was recommended as a screening tool for detecting suspected mucormycosis and/or aspergillosis samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Agata Anna Cisek, Iwona Bak, Bozena Cukrowska
Summary: Methanogenic archaea are important but often ignored in studies of human and animal intestinal microbiota. This research focused on improving the quantification of methanogens using qPCR, by refining the protocol and increasing the specificity and sensitivity of the assay. The results showed increased detection range and successful validation parameters, allowing for improved detection and quantification of methanogens in stool samples, such as chicken droppings in this study.
Article
Multidisciplinary Sciences
Xiujie Yin, Taotao He, Kun Yi, Yihang Zhao, Yao Hu, Jiaxue Liu, Xiaomeng Zhang, Lingdong Meng, Lina Wang, Haoyue Liu, Yonggang Li, Guowen Cui
Summary: Twelve reference genes were selected based on transcriptomic data for stable expression analysis in various organs of Caucasian clover under different stress conditions. Results showed that different reference genes were most stable under different stress conditions, with RCD1 and NLI2 identified as the most stable reference genes across all organs.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Jeongah Song, Jeonghee Cho, Jeongsik Park, Jeong Ho Hwang
Summary: This study identified stable reference genes in seven tissues across four developmental stages in minipigs, which can be used to evaluate mRNA expression levels. HPRT1 and 18S were found to be the most stable genes. The expression levels of ACE2 in the intestine and kidney increased significantly at PND28, consistent with the ACE2 expression pattern in humans.
Article
Genetics & Heredity
Li-Chun Jiang, Liang-Ke Gou, Xin Zhang, Qing-Mei Zhao, Shuai Tan, Rui Peng, Yu-Qing Wei, Fang-Dong Zou
Article
Engineering, Environmental
Kun Qiao, Yanbao Tian, Zhangli Hu, Tuanyao Chai
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Kun Qiao, Fanhong Wang, Shuang Liang, Hong Wang, Zhangli Hu, Tuanyao Chai
SCIENTIFIC REPORTS
(2019)
Article
Engineering, Environmental
Kun Qiao, Shuang Liang, Fanhong Wang, Hong Wang, Zhangli Hu, Tuanyao Chai
JOURNAL OF HAZARDOUS MATERIALS
(2019)
Article
Agriculture, Multidisciplinary
Kun Qiao, Fanhong Wang, Shuang Liang, Hong Wang, Zhangli Hu, Tuanyao Chai
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Engineering, Environmental
Siqi Tian, Shuang Liang, Kun Qiao, Fanhong Wang, Yuxiu Zhang, Tuanyao Chai
JOURNAL OF HAZARDOUS MATERIALS
(2019)
Article
Genetics & Heredity
Lei Zhang, Zhiheng Wang, Yuqing Wei
Summary: Dracocephalum heterophyllum is a medicinal plant with a chloroplast genome sequence of 150,860 bp. The genome contains 133 genes, including 88 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. Phylogenetic analysis indicates a close relationship between D. heterophyllum, D. moldavica, and D. palmatum.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Genetics & Heredity
Lei Zhang, Zhiheng Wang, Linxin Chang, Yuqing Wei
Summary: Peganum nigellastrum is a medicinal plant with a chloroplast genome length of 160,066 bp, containing 132 complete genes, and it has the closest relationship with P. harmala according to phylogenetic analysis.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Biochemistry & Molecular Biology
Fanhong Wang, Kun Qiao, Huanhuan Wang, Hong Wang, Tuanyao Chai
Summary: In this study, a Mn-CDF transporter called TuMTP8 was identified in diploid wheat. It was found to play a crucial role in regulating Mn distribution and homeostasis, as well as participating in the intracellular transport and storage of excess Mn, thereby conferring Mn tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Jianhui Chen, Tiran Huang, Haili Fan, Fan Lin, Hongping Ma, Jie Cao, Tuanyao Chai, Lanqing Ma, Hong Wang
Summary: In this study, the complete chloroplast genomes of three R. japonica were sequenced and analyzed. The results showed a highly conserved structure of the chloroplast genomes, with detailed annotations of the genes. Phylogenetic and genetic structure analyses revealed the evolutionary characteristics and genetic diversity of R. japonica, and potential molecular markers were identified. This study provides important genetic resources for further development and utilization of R. japonica germplasm, and offers new insights into the evolutionary characteristics of this medicinal plant.
Article
Biochemistry & Molecular Biology
Jing Li, Baolan Zhang, Penggen Duan, Li Yan, Haiyue Yu, Limin Zhang, Na Li, Leiying Zheng, Tuanyao Chai, Ran Xu, Yunhai Li
Summary: This article reports that the ERAD-related E2-E3 enzyme pair, SMG3 and DGS1, regulates grain size and weight in rice through the brassinosteroid signaling pathway. SMG3 is a homolog of UBIQUITIN CONJUGATING ENZYME 32 in Arabidopsis and interacts with DGS1, another grain size regulator. DGS1 functions as an active E3 ubiquitin ligase and colocalizes with SMG3 in the ER. SMG3 and DGS1 are involved in BR signaling and affect the accumulation of the BR receptor BRI1. The findings suggest that SMG3, DGS1, and BRI1 act together to regulate grain size and weight in rice, shedding light on the role of ERAD in grain size control and BR signaling.
Article
Plant Sciences
Ti-Ran Huang, Jian-Hui Chen, Kim E. Hummer, Lawrence A. Alice, Wen-He Wang, Yi He, Sheng-Xiang Yu, Ming-Feng Yang, Tuan-Yao Chai, Xiang-Yun Zhu, Lan-Qing Ma, Hong Wang
Summary: This article provides a comprehensive phylogenetic analysis of the genus Rubus using an integrative evidence approach. The authors propose a revised subgeneric framework and identify diagnostic morphological characters for each subgenus. This new classification system is the first systematic and complete global revision of Rubus since Focke's treatment and has important theoretical and practical significance for the development and utilization of these important agronomic crops.
Review
Chemistry, Medicinal
Jin Yang, Yu-qing Wei, Jian-bao Ding, Yan-long Li, Jian-long Ma, Jian-li Liu
CHINESE HERBAL MEDICINES
(2018)