Article
Biochemistry & Molecular Biology
Santosh G. Lavhale, Rakesh S. Joshi, Yashwant Kumar, Ashok P. Giri
Summary: Plant 4-coumarate-CoA ligase (4CL) catalyzes the ligation of CoA to cinnamic acid and its derivatives. Okimum kilimandscharicum 4CLs were characterized, showing diversity and potential classification into different classes based on phylogenetic analysis. Two selected Ok4CLs, Ok4CL7 and Ok4CL15, were further characterized in terms of gene expression, enzyme activities, substrate preferences, and potential roles. Further exploration is needed to fully understand the diverse functions of Ok4CLs in O. kilimandscharicum.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Mohammad Raghibul Hasan, Bader Saud Alotaibi, Ziyad Mohammed Althafar, Ahmed Hussain Mujamammi, Jafar Jameela
Summary: In most cases, cancer develops due to abnormal cell growth and subsequent tumour formation. Because of limitations in current treatment options, natural compounds are being investigated as potential alternatives. Around 30 natural compounds are currently in clinical trials for cancer treatment. Tulsi, or Holy Basil, is an inexpensive and widely available medicinal plant with significant religious and medicinal importance in India. The essential oil of Tulsi contains bioactive compounds such as camphor, eucalyptol, eugenol, alpha-bisabolene, beta-bisabolene, and beta-caryophyllene, which are proposed to contribute to its antimicrobial properties. Tulsi leaves, rich in eugenol, have shown anticancer properties and are being evaluated in ongoing clinical trials.
Review
Oncology
Saima Khatoon, Nida Kalam, Vinod R. M. T. Balasubramaniam, Mohd Farooq Shaikh, Mohammad Tahir Ansari
Summary: Ocimum sanctum, also known as Holy Basil, is a sacred herb of India with remarkable therapeutic efficacy. It contains polyphenols that have anti-cancer effects, but their poor bioavailability limits their clinical use. Nanonization has been implemented to improve their bioavailability and anticancer action.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Sulochana Kaushik, Samander Kaushik, Lalit Dar, Jaya Parkash Yadav
Summary: This study discovered that eugenol from Ocimum sanctum has high anti-dengue activity and can bind well with dengue proteins. Eugenol has the potential to be an important source for future dengue treatment medications.
Article
Biochemistry & Molecular Biology
Vaishnavi Amarr Reddy, Chunhong Li, Kumar Nadimuthu, Jessica Gambino Tjhang, In-Cheol Jang, Sarojam Rajani
Summary: The study identified multiple eugenol synthases in sweet basil, with ObEGS1 showing highest expression in peltate glandular trichomes and ObEGS4 in roots. Eugenol was produced in roots of soil-grown plants, but not in roots of aseptically-grown plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Xiaomin Deng, Bin Shi, Ziling Ye, Man Huang, Rong Chen, Yousheng Cai, Zhaolin Kuang, Xiang Sun, Guangkai Bian, Zixin Deng, Tiangang Liu
Summary: This study systematically screened and identified two sesquiterpenoid biosynthesis genes from Holy basil in India, successfully recognizing (-)-eremophilene and achieving high-yield bioproduction through a yeast system, while also confirming its insect-repelling effect.
METABOLIC ENGINEERING
(2022)
Article
Plant Sciences
Himanshi Tomar, Avneesh Rawat, Kirti Nagarkoti, Om Prakash, R. M. Srivastava, Shilpi Rawat, D. S. Rawat
Summary: The aim of this study was to investigate the phytochemical analysis and biological activities of essential oils from Ocimum gratissimum L. and Ocimum sanctum L. The oils were found to have antioxidant, anti-inflammatory, and α-amylase inhibitory activities. They also showed antifeedant activity against Spilosoma obliqua. In addition, in-silico studies demonstrated drug similarity and potential pharmacological activity. This study provides scientific evidence for the various biological activities and ethnomedicinal uses of the oils.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Agricultural Engineering
Ilker Turkay, Lokman Ozturk
Summary: This study investigated the effects of vermicompost on phenylpropanoid metabolism in basil. The results showed that vermicompost can regulate the accumulation of important phenylpropene compounds in basil, which affect its aroma and antimicrobial activity.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agricultural Engineering
Kajal Kumari, Sanjeev Kumar, Prashant, Amrit Kumar Jha, Nitish Kumar
Summary: This review paper discusses the application of biotechnological techniques for enhancing the production of secondary metabolites in O. sanctum. By using modern technology, the pharmaceutical yield of this plant can be improved, and studies on gene transfer and genome structure have been conducted.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Parmeshwar Lal Saran, Hiteksha I. Damor, Mohan Lal, Rohan Sarkar, Kuldeepsingh A. Kalariya, Manish Kumar Suthar
Summary: Clove basil is an aromatic herb grown in tropical and sub-tropical regions, known for its broad leaves and rich essential oil content. The experiment evaluated three diverse accessions of the plant and found significant variation in leaf morphology, chemical composition, and genetic makeup. The accessions were found to be rich in eugenol, along with other major compounds such as alpha-bergamotene and caryophyllene.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agronomy
Mukund R. Shukla, Annaliese Kibler, Christina E. Turi, Lauren A. E. Erland, J. Alan Sullivan, Susan J. Murch, Praveen K. Saxena
Summary: Tulsi, a sacred plant in many cultures, is known for its medicinal and spiritual significance with antioxidant phytochemicals. This study screened a large seed population to select lines with high antioxidant potential and standardized protocols for phytochemically consistent crop production. The integrated approach of in vitro selection and propagation shows potential for developing safe, effective, and novel natural health products.
Article
Chemistry, Physical
Vinod Kumar Meena, Vijay Kumar, Shivani Karalia
Summary: The natural extract of Ocimum sanctum (OS) has been found to inhibit the aggregation of alpha-Synuclein (alpha-Syn), suggesting a potential therapeutic intervention against Parkinson's disease (PD) and other aggregation-related disorders. The study demonstrates the inhibitory and disaggregation effects of OS extract on alpha-Synuclein, as well as its protective effect on neuroblastoma cells against fibril-induced cytotoxicity. Docking studies also indicate the binding of OS extract with alpha-Synuclein, paving the way for further research on potential therapeutic strategies.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Agricultural Engineering
Shubham Srivastava, R. K. Lal, Ranjana Maurya, Anand Mishra, Anju Kumari Yadav, Gyanesh Pandey, P. K. Rout, C. S. Chanotiya
Summary: This study focused on investigating the chemical variations among seven genotypes of Ocimum viride over three consecutive years, revealing distinct differences in chemical constituents among different genotypes, which can help to better estimate essential oil yield and chemical diversity.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Environmental Sciences
Han Jiang, Selvam Sathiyavimal, Liping Cai, Sandhanasamy Devanesan, Shaban R. M. Sayed, G. K. Jhanani, Junqing Lin
Summary: This study successfully synthesized cost-effective cobalt nanoparticles using the medicinal plant Tulsi, and evaluated their efficiency in removing methyl orange dye in wastewater treatment. The anti-inflammatory and anti-cancer properties of Tulsi-mediated cobalt nanoparticles were also examined for potential medical applications. The morphological analysis showed that the synthesized cobalt nanoparticles were crystal-shaped with an average particle size of 110 nm. In batch adsorption studies, the optimal conditions for removing methyl orange dye were found to be an incubation period of 5 hours, pH 2, temperature of 70 degrees C, and an adsorbent dosage of 125 μg/mL. Protein denaturation and nitric oxide scavenging assays demonstrated the anti-inflammatory properties of Tulsi-mediated cobalt nanoparticles, with the maximum response observed at a concentration of 250 μg/mL. MTT assays against MDA-MB-231 human breast cancer cells were used to evaluate the anti-cancer properties of the cobalt nanoparticles. This study explores the economical extraction of cobalt nanoparticles from Tulsi and their potential applications in wastewater purification and biomedical fields.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ashutosh Joshi, Gajendra Singh Jeena, Shikha, Ravi Shankar Kumar, Alok Pandey, Rakesh Kumar Shukla
Summary: OscWRKY1 positively regulates phenylpropanoid pathway genes and rosmarinic acid content, enhancing resistance against bacterial pathogen in Arabidopsis.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Environmental Sciences
Madhuri Gupta, Prashant Kumar, Praveen Kumar Sharma, Shubham Srivastava, Priyabrat Mohapatra, Chandan Singh Chanotiya, Prasant Kumar Rout
Summary: Monoterpene hydrocarbons are unwanted components in essential oils, but can be converted into valuable compounds using a solvent-free process. This study aims to develop an eco-friendly strategy for the valorization of essential oils rich in bicyclic monoterpene hydrocarbons.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Agricultural Engineering
Narendra Kumar, Dipayan Ghosh, Neha Chaudhary, Chandan Singh Chanotiya
Summary: This study analyzed the premature senescence induced by rainfall in Pelargonium graveolens. The results showed that with the progression of senescence, chlorophyll content increased and anthocyanin content decreased. Additionally, protein content, oxidative stress-related parameters increased, cell viability decreased, and gland density reduced. Gas chromatography and gas chromatography-mass spectrometry analysis revealed that senescence significantly modulates oil constituents and yield.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
R. K. Lal, Pankhuri Gupta, Anand Mishra, C. S. Chanotiya
Summary: This study aims to increase the yield and quality of basil essential oil by gathering different genes and estimating genetic parameters. The analysis shows that the three-way crosses exhibit high variations for oil yield and yield contributing characters.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Green & Sustainable Science & Technology
Ashween Deepak Nannaware, Ch Mohan Sai Kumar, Shubham Srivastava, Suman Singh, Manglesh Kumar Gupta, Prasanta Kumar Rout, C. S. Chanotiya, R. K. Lal, Yogesh Nimdeo, Saumendu Roy
Summary: This study designed and fabricated a solar-operated distillation unit (SDU) for the extraction of valuable essentials from aromatic crops. The SDU demonstrated improved essential oil quality and increased biomarker content compared to traditional wood-based distillation operation.
Article
Environmental Sciences
Shivani Chaturvedi, Prashant Kumar, Deepak Kumar, Naziya Syed, Madhuri Gupta, Chandan Singh Chanotiya, Prasant Kumar Rout, Sunil Kumar Khare
Summary: In this study, single cell oil (SCO) was produced using food waste as a carbon source and a natural plant extract as an inhibitor, achieving the goal of increasing lipid accumulation through a natural approach.
WASTE AND BIOMASS VALORIZATION
(2022)
Article
Biochemistry & Molecular Biology
R. K. Lal, C. S. Chanotiya, P. Gupta, A. Mishra
Summary: This study investigated the essential oil yield of 80 vetiver lines in different environments. The genotype and genotype x environment interactions had a significant impact on essential oil yield. Three genotypes performed consistently well in different environments and were favorable for essential oil production. The color of vetiver inflorescence was associated with different essential oil components, and these marker associations could be useful for selecting genotypes with high yields.
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
(2022)
Article
Plant Sciences
Pamela Lemos Cruz, Ines Carqueijeiro, Konstantinos Koudounas, Dikki Pedenla Bomzan, Emily Amor Stander, Cecile Abdallah, Natalja Kulagina, Audrey Oudin, Arnaud Lanoue, Nathalie Giglioli-Guivarc'h, Dinesh A. Nagegowda, Nicolas Papon, Sebastien Besseau, Marc Clastre, Vincent Courdavault
Summary: A second isoform of the 16-hydroxytabersonine O-methyltransferase (16OMT) has been identified, which acts on both MIAs and flavonoid substrates, suggesting that the enzyme has evolved to accommodate the MIAs substrate. The results contribute to our understanding of the evolutionary processes that have led to the co-optation of enzymes for MIA synthesis.
Article
Chemistry, Physical
Prashant Kumar, Praveen Kumar Sharma, Shivani Chaturvedi, Chandan Singh Chanotiya, Prema Ranjan Rauta, Priyabrat Mohapatra, Prasant Kumar Rout
Summary: The catalytic process of transforming citral to enantiospecific (+)-citronellal increases the fragrance value of lemongrass essential oil and expands its potential applications.
Article
Chemistry, Multidisciplinary
Prashant Kumar, Praveen Kumar Sharma, Shivani Chaturvedi, Suman Singh, Shreya Tripathi, Huma Fatima, Minakshi Grover, Priyabrat Mohapatra, ChandanSingh Chanotiya, Prasant Kumar Rout
Summary: The current research focuses on using waste materials to produce nanoparticles in an environmentally friendly way. In this study, the discarded aqueous portion of Prosopis cineraria pod was used to synthesize Ag and Ag-Cu nanoparticles, which showed small size and antimicrobial properties. Furthermore, these nanoparticles were found to be effective catalysts for the epoxidation of alkene moieties, particularly Ag-Cu nanoparticles with copper-facilitated epoxidation. This inexpensive and green method of producing nanoparticles has potential applications in various industries and medicine.
RESEARCH ON CHEMICAL INTERMEDIATES
(2023)
Article
Environmental Sciences
Prashant Kumar, Chandan Singh Chanotiya, Laldingngheti Bawitlung, Anju Yadav, Pankaj Kumar, Anirban Pal, Ajit Kumar Shasany, Priyabrat Mohapatra, Prasant Kumar Rout
Summary: A two-step greener catalytic process has been developed using novel bi-acidic composites to semi-synthesize (-)-menthol from citronellal-rich essential oils. The composite Sn-B-NaYZE showed enhanced selectivity due to its higher Lewis and Bronsted acidic characteristics. The purified (-)-menthol obtained from this process was found to be 100% biobased and nature-identical.
WASTE AND BIOMASS VALORIZATION
(2023)
Review
Green & Sustainable Science & Technology
Ch Mohan Sai Kumar, Suman Singh, Manglesh Kumar Gupta, Yogesh M. Nimdeo, Ravi Raushan, Ankit Deorankar, T. M. Ananda Kumar, Prasant Kumar Rout, C. S. Chanotiya, Vinod D. Pakhale, Ashween Deepak Nannaware
Summary: India, as the fifth largest economy in the world, relies on agriculture and allied sector for 20% of its GDP. To meet the energy demand in agriculture, renewable solar energy has emerged as a prime source to reduce dependency on conventional energy. Solar energy has been recognized as a promising alternative for heat and electricity in agriculture. This review focuses on the status, importance, availability, and applications of solar energy technologies in agricultural operations in India.
SUSTAINABLE ENERGY TECHNOLOGIES AND ASSESSMENTS
(2023)
Article
Agricultural Engineering
Pooja Gaur, Feroz Khan, Karuna Shanker
Summary: This study successfully isolated biologically active compounds from Andrographis paniculata root that have the ability to absorb dietary fat, making them potential candidates for obesity management. 7-O-methyl-dihydro wogonin (MDHW) exhibited the strongest pancreatic lipase inhibition activity and showed a synergistic effect with orlistat. MDHW acts as a competitive inhibitor through energy transfer from pancreatic lipase, and the binding interaction between MDHW and pancreatic lipase is hydrophobic. In vitro and in silico studies confirmed the potential of MDHW as an anti-obesity agent for pharmaceutical and food industry applications.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Analytical
Pooja Gaur, Princi Gupta, Neerja Tiwari, Namita Gupta, Karuna Shanker
Summary: A novel HPTLC method was developed for the simultaneous quantification of six bioactive alkaloids in agro-waste of Piper longum. The method demonstrated excellent separation and specificity, and was successfully applied for accurate quantification in Piper longum roots. Additionally, the method was found to be environmentally friendly and energy efficient.
MICROCHEMICAL JOURNAL
(2023)
Article
Plant Sciences
Maneesha Mall, Karuna Shanker, Dinesh A. Nagegowda, Abdul Samad, Alok Kalra, Alok Pandey, Velusamy Sundaresan, Ashutosh K. Shukla
Summary: Plant lipocalins have various functions. A temperature-induced lipocalin (CrTIL) was studied to understand its role in maintaining cell membrane integrity and its relationship with vindoline content. The study confirmed that CrTIL is involved in maintaining membrane stability and showed an inverse relationship with vindoline content. This research provides insights into the importance of CrTIL in intracellular sequestration, storage, and viability of vindoline.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Agriculture, Multidisciplinary
Pooja Gaur, Neerja Tiwari, Karuna Shanker
Summary: This study demonstrates the potential of using agricultural wastes of Piper longum as antiobesity agents through the extraction of pseudoalkaloids. One of the compounds, pellitorine, showed strong pancreatic lipase inhibitory activity and exhibited synergistic effects with orlistat. The structural compactness of the interaction between pellitorine and pancreatic lipase was confirmed by Fourier transform infrared spectral studies. Molecular docking studies further revealed the binding interaction between pellitorine and the catalytic active amino side residue of the protein. These findings suggest that pellitorine has the potential to be developed as an anti-obesity supplement.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2022)
