Article
Biochemistry & Molecular Biology
Qiaoyun Chu, Ping Liu, Yihan Song, Ronghui Yang, Jing An, Xuewei Zhai, Jing Niu, Chuanzhen Yang, Binghui Li
Summary: Reprogramming of lipid metabolism is a hallmark of cancer, but the involvement of specific fatty acids and enzymes in tumorigenesis is not well understood. This study shows that depletion of the enzyme acetyl-CoA carboxylase inhibits the synthesis and elongation of very long-chain fatty acids in human cancer cells. Deficiency of very long-chain fatty acids impairs mitochondrial morphology and makes cancer cells susceptible to oxidative stress and cell death.
Article
Plant Sciences
Yongtai Yin, Nadia Raboanatahiry, Kang Chen, Xinfeng Chen, Tian Tian, Jia Jia, Hongsheng He, Jianjie He, Zhenyi Guo, Longjiang Yu, Maoteng Li
Summary: This study discovered that LPAT2 from Camelina sativa can promote the accumulation of very long-chain fatty acids (VLCFAs) in oil crops. Overexpression of LPAT2 significantly increased the VLCFA content in seeds without affecting oil content. The function of LPAT2 in promoting VLCFA accumulation is conserved in other oil crops. These findings provide a promising gene resource for VLCFA production.
Article
Biochemistry & Molecular Biology
Honoka Tanno, Takayuki Sassa, Megumi Sawai, Akio Kihara
Summary: Branched-chain fatty acids are important components in the meibum lipids secreted from the meibomian glands. Enzymes such as ELOVL1, ELOVL3, and ELOVL7 are responsible for elongating branched-chain acyl-CoAs. Liquid chromatography-tandem mass spectrometry has revealed the predominance of branched-chain lipids in mouse meibomian glands, demonstrating the significance of ELOVL1 in elongating branched-chain VLC acyl-CoAs in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Plant Sciences
Juyoung Kim, Ryeo Jin Kim, Saet Buyl Lee, Mi Chung Suh
Summary: Interactions between KCS9 and other proteins in the fatty acid elongase complex are essential for the synthesis of very-long-chain fatty acids (VLCFAs). This study provides insights into the quaternary structure and protein-protein interactions of the fatty acid elongase complex, highlighting their importance for efficient VLCFA synthesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Tianquan Yang, Youhan Li, Yu Liu, Liangliang He, Aizhong Liu, Jiangqi Wen, Kirankumar S. Mysore, Million Tadege, Jianghua Chen
Summary: This study revealed the crucial role of a 3-ketoacyl-CoA synthase (KCS) enzyme involved in the biosynthesis of very long chain fatty acids and cuticular wax in the aerial organ development of Medicago truncatula. Mutants with defects in cuticular wax showed altered leaf surface wax composition and developmental defects.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Xiaobo Zhang, Ying Wang, Xiaowen Wang, Zhu Zhu, Xuefei Zhang, Luqi Jia, Yangyang Li, Weijiang Tian, Hongyan Chen, Xiaoyan Zhu, Guanghua He, Xianchun Sang
Summary: A semi-dwarf mutant, sd38, with significantly reduced cell length was identified in rice. The mutation of the SD38 gene, which is involved in the synthesis of very-long-chain fatty acids, affected lipid metabolism and ethylene synthesis. Exogenously supplied VLCFA (C24:0) increased the expression levels of ethylene synthesis-related genes and the plant height of sd38 mutant seedlings.
Article
Plant Sciences
Hui Liu, Xue-Mei Yan, Xin-rui Wang, Dong-Xu Zhang, Qingyuan Zhou, Tian-Le Shi, Kai-Hua Jia, Xue-Chan Tian, Shan-Shan Zhou, Ren-Gang Zhang, Quan-Zheng Yun, Qing Wang, Qiuhong Xiang, Chanaka Mannapperuma, Elena Van Zalen, Nathaniel R. Street, Ilga Porth, Yousry A. El-Kassaby, Wei Zhao, Xiao-Ru Wang, Wenbin Guan, Jian-Feng Mao
Summary: This study focused on in-depth genome characterization of the oil-producing tree yellowhorn, using long-read sequencing technologies to assemble a highly contiguous genome. The research identified the centromere structure and evolution, fatty acid biosynthesis, as well as comparative genomics among different cultivars. The study provides valuable genomic resources for further research on centromere structure and evolution, and functional studies in oil-producing plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yuko Fujiwara, Kotaro Hama, Nobuyuki Shimozawa, Kazuaki Yokoyama
Summary: In this study, we analyzed the glycosphingolipid (GSL) species in fibroblasts from X-ALD patients and found elevated levels of specific GSLs. Quantitative analysis was carried out using liquid chromatography-electrospray ionization-tandem mass spectrometry technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Yuecheng Xi, Lena Lindenmayer, Ian Kline, Jens von Einem, John G. Purdy
Summary: The study demonstrates that stress and virus infection can regulate lipid metabolism, with PERK playing a crucial role in promoting the levels of phospholipids with saturated/monounsaturated very-long-chain FA tails during HCMV infection. Additionally, PERK is essential for virus replication and infectivity, highlighting its importance in HCMV pathogenesis.
Review
Biochemistry & Molecular Biology
Pia Erdbruegger, Florian Froehlich
Summary: VLCFAs are highly diverse molecules with variable chain length, double bond numbers, and hydroxylation sites, synthesized in the ER through membrane embedded enzymes. Mis-regulation of VLCFA metabolism can lead to inherited diseases like ichthyosis and demyelination. Recent studies in yeast suggest intricate regulation of VLCFA metabolism for lipid homeostasis.
BIOLOGICAL CHEMISTRY
(2021)
Article
Nutrition & Dietetics
Fabian A. Vogelpohl, Antonio W. Gomes-Neto, Ingrid A. Martini, Camilo G. Sotomayor, Dion Groothof, Maryse C. J. Oste, Margaretha Rebecca Heiner-Fokkema, Frits A. J. Muskiet, Stefan P. Berger, Gerjan Navis, Ido P. Kema, Stephan J. L. Bakker
Summary: In kidney transplant recipients (KTR), very long chain saturated fatty acids (VLSFA) are inversely associated with infectious disease mortality, especially behenic acid (C22:0) showing a reverse correlation with all-cause mortality risk.
Review
Nutrition & Dietetics
Kira Zhi Hua Lai, Nagam A. Yehia, Zhila Semnani-Azad, Sonia Blanco Mejia, Beatrice A. Boucher, Vasanti Malik, Richard P. Bazinet, Anthony J. Hanley
Summary: Recent observational studies suggest that circulating very long-chain saturated fatty acids (VLCSFAs) are inversely associated with cardiometabolic outcomes. This review aimed to assess the effects of diet, physical activity, and smoking on circulating VLCSFAs; however, the limited evidence and methodological limitations of the included studies make it unclear.
ADVANCES IN NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Koichiro Tsuchiya, Kazunori Sango, Tamotsu Tanaka
Summary: One of the major functions of peroxisomes in mammals is to oxidize very long-chain fatty acids (VLCFAs), which if accumulated due to genetic defects in peroxisomal β-oxidation, can lead to various health problems. The biological effect of extracellular VLCFAs was characterized using a solubilizing technique in peroxisome-deficient CHO cells, PC12 cells, and IFRS1 cells. The study found that C20:0 fatty acid was the most toxic among the tested C16-C26 FAs, causing apoptosis in cells. Peroxisomes play a crucial role in detoxifying apoptotic VLCFAs by preventing their accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Nutrition & Dietetics
Qixin Wu, Dandan Shi, Ting Dong, Zhuolin Zhang, Qingjian Ou, Yujing Fang, Caixia Zhang
Summary: This study investigated the association between circulating saturated fatty acids (SFAs) including very long-chain SFAs (VLCSFAs) and the risk of colorectal cancer (CRC). The results showed that higher levels of total SFAs were associated with an increased risk of CRC, while higher levels of VLCSFAs were associated with a decreased risk of CRC. Specifically, certain types of SFAs like lauric acid, myristic acid, palmitic acid, heptadecanoic acid, and arachidic acid were positively associated with CRC risk.
Article
Agriculture, Dairy & Animal Science
Han Wang, Moran Hu, Zhonghao Shen, Xiaolong Zhou, Songbai Yang, Ke He, Xiangchen Li, Feifei Yan, Ayong Zhao
Summary: This study demonstrates that miR-22 regulates fatty acid composition and mitochondrial morphology in skeletal muscle cells by inhibiting ELOVL6 expression. The findings provide valuable information for controlling muscle lipid accumulation and mitochondrial function in livestock.
Article
Agronomy
Takanori Yoshikawa, Shin-Ya Tanaka, Yuuki Masumoto, Naoya Nobori, Hiroto Ishii, Ken-Ichiro Hibara, Jun-Ichi Itoh, Takatoshi Tanisaka, Shin Taketa
Article
Developmental Biology
Jun-Ichi Itoh, Yutaka Sato, Yutaka Sato, Ken-Ichiro Hibara, Sae Shimizu-Sato, Hiromi Kobayashi, Hinako Takehisa, Karen A. Sanguinet, Nobukazu Namiki, Yoshiaki Nagamura
Article
Developmental Biology
Ken-ichiro Hibara, Miyako Isono, Manaki Mimura, Naoki Sentoku, Mikiko Kojima, Hitoshi Sakakibara, Yuka Kitomi, Takanori Yoshikawa, Jun-ichi Itoh, Yasuo Nagato
Article
Biotechnology & Applied Microbiology
Tomomi Abe, Ryota Iiyoshi, Kyosuke Ito, Yutaka Takahashi, Kohei Sato, Atsushi Matsuzaka, Tomonori Sonoki, Yukihiro Ito
PLANT BIOTECHNOLOGY
(2016)
Article
Plant Sciences
Toshiki Ishikawa, Yukihiro Ito, Maki Kawai-Yamada
Article
Biochemistry & Molecular Biology
Yoichi Hashida, Tatsuro Hirose, Masaki Okamura, Ken-ichiro Hibara, Ryu Ohsugi, Naohiro Aoki
Article
Plant Sciences
Eriko Honda, Chow-Lih Yew, Takanori Yoshikawa, Yutaka Sato, Ken-ichiro Hibara, Jun-Ichi Itoh
PLANT AND CELL PHYSIOLOGY
(2018)
Article
Biochemical Research Methods
Ryota Iiyoshi, Taichi Oguchi, Toru Furukawa, Yosuke Iimura, Yukihiro Ito, Tomonori Sonoki
TRANSGENIC RESEARCH
(2017)
Article
Developmental Biology
Satomi Shimano, Ken-ichiro Hibara, Tomoyuki Furuya, Shin-ichi Arimura, Hirokazu Tsukaya, Jun-Ichi Itoh
Article
Biotechnology & Applied Microbiology
Masashi Naruse, Honami Takahashi, Nori Kurata, Yukihiro Ito
PLANT BIOTECHNOLOGY
(2018)
Article
Multidisciplinary Sciences
Masaru Fujimoto, Takashi Sazuka, Yoshihisa Oda, Hiroyuki Kawahigashi, Jianzhong Wu, Hideki Takanashi, Takayuki Ohnishi, Jun-ichi Yoneda, Motoyuki Ishimori, Hiromi Kajiya-Kanegae, Ken-ichiro Hibara, Fumiko Ishizuna, Kazuo Ebine, Takashi Ueda, Tsuyoshi Tokunaga, Hiroyoshi Iwata, Takashi Matsumoto, Shigemitsu Kasuga, Jun-ichi Yonemaru, Nobuhiro Tsutsumi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Developmental Biology
Kiyoe Ishimoto, Shino Sohonahra, Mitsuko Kishi-Kaboshi, Jun-ichi Itoh, Ken-ichiro Hibara, Yutaka Sato, Tsuneaki Watanabe, Kiyomi Abe, Akio Miyao, Misuzu Nosaka-Takahashi, Toshiya Suzuki, Nhung Kim Ta, Sae Shimizu-Sato, Takamasa Suzuki, Atsushi Toyoda, Hirokazu Takahashi, Mikio Nakazono, Yasuo Nagato, Hirohiko Hirochika
Article
Genetics & Heredity
Ken-Ichiro Hibara, Masayuki Miya, Sean Akira Benvenuto, Naoko Hibara-Matsuo, Manaki Mimura, Takanori Yoshikawa, Masaharu Suzuki, Makoto Kusaba, Shin Taketa, Jun-ichi Itoh
Summary: This study investigated the genetic mechanisms of plastochron control in grass species using many-noded dwarf mutants in barley. The MND genes were found to play important roles in regulating leaf growth and plastochron independently in barley. The study revealed both conserved and diverse aspects of plastochron regulation between barley and rice.
Article
Biochemistry & Molecular Biology
Keita Kogure, Akiko Watanabe, Yukihiro Ito
Summary: This study identified ketoacyl CoA reductase (KCR) as an interacting partner of ONI1 and ONI2. The results suggest that ONI2 and KCR1 may form an FAE complex that plays a role in biosynthesizing very-long-chain fatty acids during shoot development in rice.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Multidisciplinary Sciences
Akiko Watanabe, Yoshino Hatanaka, Yukino Takeshima, Karin Sasaki, Noa Takahashi, Yukihiro Ito
Summary: This study demonstrates that rice seedlings grown in darkness can produce a comparable amount of soluble protein to those grown in light. Optimal conditions for protein production in dark-grown rice seedlings include large seeds, germination and growth at 28 degrees C, supplementation with specific medium and sucrose, and dense planting. Therefore, this research suggests the possibility of producing foreign proteins in rice seedlings in the dark, reducing electricity usage and increasing cost-effectiveness.
SCIENTIFIC REPORTS
(2022)