Article
Biochemistry & Molecular Biology
Nina Djapic
Summary: Supercritical carbon dioxide extraction is used to obtain chemical compounds, including nicotine, from N. tabacum leaves. Optimal extraction conditions were determined using response surface methodology, with pressure, temperature, and time affecting extraction yield and nicotine content. Results show that pressure, temperature, and time play significant roles in the extraction process for different N. tabacum varieties.
Article
Environmental Sciences
Mariana Guadalupe Abrile, Maria Laura Fiasconaro, Daiana Soledad Orecchia, Ricardo Martin Manzo, Maria Eugenia Lovato
Summary: The study evaluated the potential of LS as an organic amendment, showing that its application significantly improved plant growth parameters and increased protein and photosynthetic pigment content.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Plant Sciences
Jishun Zhang, Xiaolian Zhang, Mengao Jia, Qiang Fu, Yushuang Guo, Zhihong Wang, Dejun Kong, Yingchao Lin, Degang Zhao
Summary: The NRAMP6a and NRAMP6b genes in tobacco play important roles in metal transport and tolerance. They reduce cadmium and manganese accumulation in tobacco shoots by influencing root-to-shoot translocation, leading to higher chlorophyll content and lighter leaf damage.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Review
Plant Sciences
Tsubasa Shoji, Takashi Hashimoto, Kazuki Saito
Summary: Tobacco is a widely cultivated crop, but its highly addictive nature poses a significant risk to human health. Recent research in molecular and genomics has led to advancements in understanding the metabolic and regulatory genes involved in nicotine biosynthesis, allowing the development of tobacco plants with ultra-low nicotine levels through mutational breeding, genetic engineering, and genome editing.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Genetics & Heredity
Yalin Zheng, Yudong Chen, Zhiguo Liu, Hui Wu, Fangchan Jiao, Haiping Xin, Li Zhang, Long Yang
Summary: Nicotiana alata, an ornamental horticultural plant, displays a variety of flower colors and a long flowering period. Differences in flower color are explained by analyzing genes in different colored flowers, with crucial roles played by chlorophyll and anthocyanin biosynthesis pathways. Specific genes such as HEMA, F3H, F3 ' 5 ' H, DFR, FLS as well as transcription factor genes like HY5, MYB12, AN1 and AN4, are involved in determining the flower color differences.
Article
Plant Sciences
Renata Salcedo-Sanchez, Yuridia Cruz-Zamora, Felipe Cruz-Garcia
Summary: This study identified the regulatory mechanism of the self-incompatibility gene SC10-RNase in tobacco. The presence of a Gypsy element in the SC10-RNase promoter may contribute to DNA methylation remodeling, resulting in abundant expression of SC10-RNase in mature pistils.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Larisa Fedoreyeva, Inna A. Chaban, Neonila Kononenko
Summary: The addition of glycine amino acid and dipeptides to tobacco roots was found to significantly enhance the development of root hairs. Peptides accumulated in specific tissues of the root and regulated gene expression involved in root hair growth. However, the molecular mechanisms underlying this effect remain unclear and may involve interactions with DNA and histones, potentially through epigenetic regulation.
Article
Biotechnology & Applied Microbiology
Kacper Piotr Kaminski, Lucien Bovet, Aurore Hilfiker, Helene Laparra, Joanne Schwaar, Nicolas Sierro, Gerhard Lang, Damien De Palo, Philippe Alexandre Guy, Csaba Laszlo, Simon Goepfert, Nikolai V. Ivanov
Summary: This study investigates the effects of suppressing putrescine methyltransferase (PMT) and methylputrescine oxidase (MPO) on tobacco growth and alkaloid synthesis. The results show that suppressing PMT expression leads to a significant decrease in nicotine content and an increase in anatabine in transgenic plants after pruning. In transgenic plants with suppressed MPO expression, nicotine almost disappears in the leaves while anatabine content increases.
Article
Agricultural Engineering
Pingguo Yang, Patrick J. Drohan, HuaiYu Long, Miao Yang, Yun Bian, Erdeng Ma
Summary: The study found that negative pressure irrigation can improve water use efficiency and nutrient uptake efficiency, reduce water consumption, increase yield, and improve the taste of tobacco in greenhouse environments.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agronomy
Nathaniel Burner, Sheri P. Kernodle, Tyler Steede, Ramsey S. Lewis
Summary: This study successfully produced tobacco plants with A622 gene knockout using gene editing technology, and these mutant plants showed significantly reduced nicotine accumulation in field and greenhouse experiments. However, reducing A622 activity also resulted in significantly reduced growth and development of the plants.
MOLECULAR BREEDING
(2022)
Article
Agronomy
Eleni Tsaliki, Theodoros Moysiadis, Evripidis Toumpas, Apostolos Kalivas, Ioannis Panoras, Ioannis Grigoriadis
Summary: Tobacco is a widely grown crop with high adaptability to different soils and climates. The chemical composition of tobacco leaves varies among different locations, which is important for the tobacco industry. This study evaluated the effect of variety, area, and cultivation period on the yield and content of nicotine, sugar, and nitrate in tobacco leaves. The results showed significant interactions between variety, area, and cultivation year on yield and nicotine content. Sugar content was significantly affected by all factors. The study also found that certain varieties showed better adaptation and higher yield in specific regions.
Article
Microbiology
Dane Goh, Julien G. A. Martin, Claudia Banchini, Allyson M. M. MacLean, Franck Stefani
Summary: This study presents a method called RocTest that models the propagation of arbuscular mycorrhizal fungi (AMF) in root organ cultures (ROCs). The method allows for assessing the performance of different ROCs in fungal symbiont propagation and identifying the best combination of host and AMF. This method is valuable for germplasm collections and AMF research.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agronomy
Nathaniel Burner, Abigail McCauley, Sreepriya Pramod, Jesse Frederick, Tyler Steede, Sheri P. Kernodle, Ramsey S. Lewis
Summary: This research identified several tobacco introductions with low alkaloid phenotypes through phenotyping and genotyping analysis. Some introductions achieved lower alkaloid accumulation by deleting specific genes, while others achieved low alkaloid phenotypes through the deletion of another gene. In addition, a new genomic region that may affect alkaloid accumulation was discovered through quantitative trait locus mapping.
MOLECULAR BREEDING
(2022)
Article
Plant Sciences
Marcin Delijewski, Khaled Radad, Christopher Krewenka, Barbara Kranner, Rudolf Moldzio
Summary: Nicotine shows neuroprotective effects on dopaminergic neurons, increasing neurite length and count, as well as affecting glutathione levels.
Article
Environmental Sciences
Chong Liu, Dan Sun, Hong-Xiang Zheng, Guo-Bao Wang, Wen-Shen Liu, Yue Cao, Ye-Tao Tang, Rong-Liang Qiu
Summary: This study revealed the mechanism of organic acids in enhancing REE tolerance and accumulation in Phytolacca americana, showing that Yttrium stress stimulated the accumulation of oxalate, malate, and citrate, promoting the transport of Y from roots to shoots. Transcriptome analysis indicated that PaNIP1;2 may play a crucial role in the transport of Y-malate complexes within the plant.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Plant Sciences
Masaru Nakayasu, Naoki Shioya, Masahito Shikata, Chonprakun Thagun, Ayman Abdelkareem, Yoshihiro Okabe, Tohru Ariizumi, Gen-ichiro Arimura, Masaharu Mizutani, Hiroshi Ezura, Takashi Hashimoto, Tsubasa Shoji
Article
Plant Sciences
Tsubasa Shoji, Takashi Hashimoto
JOURNAL OF PLANT RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Ayman Abdelkareem, Chonprakun Thagun, Shunsuke Imanishi, Takashi Hashimoto, Tsubasa Shoji
PLANT BIOTECHNOLOGY
(2019)
Review
Plant Sciences
Tsubasa Shoji
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Plant Sciences
Shunya Hayashi, Mutsumi Watanabe, Makoto Kobayashi, Takayuki Tohge, Takashi Hashimoto, Tsubasa Shoji
PLANT AND CELL PHYSIOLOGY
(2020)
Review
Plant Sciences
Tsubasa Shoji, Ling Yuan
Summary: Plants produce structurally diverse specialized metabolites in response to environmental stresses, with the AP2/ERF family of transcription factors playing key roles in regulating biosynthesis. Research has shown that some ERF genes occur in clusters on chromosomes, with jasmonate-responsive ERF gene clusters controlling the biosynthesis of important metabolites.
TRENDS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Tsubasa Shoji, Naoyuki Umemoto, Kazuki Saito
Summary: Plant domestication and improvement have led to mutational changes in transcriptional regulators of defense metabolism. Specialized compounds like alkaloids and terpenoids play essential roles in plant defense against herbivores and microbes. Transcription factors coordinately regulate defense metabolism genes by recognizing cis-regulatory elements in the promoter regions of target genes.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Elida Zairina, Gesnita Nugraheni, Arie Sulistyarini, Mufarrihah, Catur Dian Setiawan, Sunil Kripalani, Safira Indah Lestari
Summary: The study reveals that approximately 50% of patients with type 2 diabetes do not adhere to their prescribed medication regimen, and certain barriers contribute to this non-adherence. Conducted in Surabaya, Indonesia, the study highlights a significant difference in adherence levels based on education.
JOURNAL OF DIABETES AND METABOLIC DISORDERS
(2022)
Article
Plant Sciences
Tsubasa Shoji, Koki Moriyama, Nicolas Sierro, Sonia Ouadi, Nikolai Ivanov, Takashi Hashimoto, Kazuki Saito
Summary: In this study, the researchers investigated the regulation of nicotine biosynthesis in tobacco and identified several low-nicotine tobacco varieties lacking specific transcription factors. They characterized the newly identified alleles and examined their influence on alkaloid contents and gene expression related to nicotine biosynthesis. They also demonstrated the effect of a distal genomic region deletion on the expression of a key transcription factor and the alkaloid phenotype. These findings provide new insights into the regulation of nicotine biosynthesis and offer genetic resources for breeding low-nicotine tobacco.
Article
Plant Sciences
Tsubasa Shoji, Kazuki Saito
Summary: This study provides insights into the molecular mechanism of SGA biosynthesis and its regulation in eggplant, offering a theoretical basis for further understanding of the anti-nutritional defense in eggplant plants.
Article
Public, Environmental & Occupational Health
Elida Zairina, Arie Sulistyarini, Gesnita Nugraheni, Sindy Irvana Kurnia Saputri, Ayudika Permatasari, Elinda Eka Rachmawati
Summary: This study aimed to identify healthy individuals at risk for developing type 2 diabetes. The CANRISK questionnaire was used to assess the risk factors. The results showed that age, BMI, waist circumference, gender, history of hypertension, family history of diabetes mellitus, and education were all significantly associated with the risk category using CANRISK.
JOURNAL OF PUBLIC HEALTH-HEIDELBERG
(2023)
Article
Biochemistry & Molecular Biology
Fumihito Hasebe, Honoka Yuba, Takashi Hashimoto, Kazuki Saito, Nobutaka Funa, Tsubasa Shoji
Summary: This passage discusses the biosynthesis process of tropane alkaloids in the roots of medicinal plants, outlining the key metabolic enzymes and reaction mechanisms involved. The generation of mutants through genome editing techniques demonstrates the impact of PYKS activity loss on tropane alkaloid metabolism, offering valuable insights into the role of scaffold-forming reaction in this biosynthetic pathway.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Masaru Nakayasu, Kohei Ohno, Kyoko Takamatsu, Yuichi Aoki, Shinichi Yamazaki, Hisabumi Takase, Tsubasa Shoji, Kazufumi Yazaki, Akifumi Sugiyama
Summary: This study investigated the biological function of the major saponin alpha-tomatine in the rhizosphere, revealing its impact on soil bacterial communities and suggesting additional functions in belowground biological communication. The results highlight the role of tomatine in shaping the bacterial communities of the rhizosphere.
Article
Biochemistry & Molecular Biology
Masataka Kajikawa, Nicolas Sierro, Takashi Hashimoto, Tsubasa Shoji
PLANT SIGNALING & BEHAVIOR
(2017)
Article
Biochemistry & Molecular Biology
Ayman Abdelkareem, Chonprakun Thagun, Masaru Nakayasu, Masaharu Mizutani, Takashi Hashimoto, Tsubasa Shoji
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)