期刊
PLANT AND CELL PHYSIOLOGY
卷 51, 期 4, 页码 554-560出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcq020
关键词
PSII; Psb30; PsbK; PsbZ; Thermosynechococcus elongatus; Ycf12
资金
- Japan Society for the Promotion of Science [17770042, 20370018]
- Grants-in-Aid for Scientific Research [20370018, 17770042] Funding Source: KAKEN
PsbK is a small membrane protein of the PSII core complex and is highly conserved from cyanobacteria to plants. Here, we studied its role in the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by focusing on a psbK disruptant with hexahistidine-tagged CP47. The psbK disruptant showed photoautotrophic growth comparable with that of the wild type under a wide range of light conditions. The mutant PSII complex retained the oxygen-evolving activity with a unique modification of the acceptor Q(B) site. N-terminal sequencing revealed that Ycf12 and PsbZ proteins were lost in the PSII complex prepared from the mutant. Immunoblotting detected reduced accumulation of PsbZ in the mutant thylakoid. These results suggest that PsbK is required not only for association of PsbZ and Ycf12 with the isolated PSII complex but also for the stabilization of PsbZ in the thylakoid membrane.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据