Article
Multidisciplinary Sciences
Rafael E. Venado, Lucas E. Wange, Defeng Shen, Fabienne Pinnau, Tonni Grube Andersen, Wolfgang Enard, Macarena Marin
Summary: This study provides genetic evidence for the formation of the nodule oxygen diffusion barrier in legumes, which is crucial for nitrogen fixation in root nodules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Mitsutaka Fukudome, Yuta Shimokawa, Shun Hashimoto, Yusuke Maesako, Nahoko Uchi-Fukudome, Kota Niihara, Ken-Ichi Osuki, Toshiki Uchiumi
Summary: The hmp gene from Sinorhizobium meliloti introduced into Mesorhizobium loti reduces NO levels in symbiosis with Lotus japonicus, leading to delayed infection and increased nitrogenase activity without affecting nodule senescence, contrasting with the effects of class 1 phytoglobin in L. japonicus.
MICROBES AND ENVIRONMENTS
(2021)
Article
Plant Sciences
Lucas G. Castellani, Abril Luchetti, Juliet F. Nilsson, Julieta Perez-Gimenez, Caren Wegener, Andreas Schluter, Alfred Puhler, Antonio Lagares, Susana Brom, Mariano Pistorio, Karsten Niehaus, Gonzalo A. Torres Tejerizo
Summary: Biological nitrogen fixation is a major source of nitrogen into the biosphere, achieved through symbiosis between rhizobia and leguminous plants. Exopolysaccharides play a crucial role in establishing effective symbiosis between alfalfa and Ensifer meliloti. Rhizobium favelukesii LPU83 can infect alfalfa but lacks efficient nitrogen fixation, with different EPS biosynthesis gene organization compared to E. meliloti.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Simon B. Kelly, Simon Hansen, Henriette Rubsam, Pia Saake, Emil Pedersen, Kira Gysel, Eva T. Madland, Shunliang Wu, Stephan Wawra, Dugald Reid, John Sullivan, Zuzana B. Blahovska, Maria L. Vinther, Artur W. Muszynski, Parastoo Azadi, Mikkel R. Thygesen, Finn Aachmann, Clive Ronson, Alga Zuccaro, Kasper Andersen, Simona Radutoiu, Jens Stougaard
Summary: This study identifies and characterizes a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonized by arbuscular mycorrhizal (AM) fungi and can bind glucans with a branching pattern characteristic of fungal glucans. In vitro assays show that EPR3a binds cell wall glucans and rhizobial exopolysaccharides with affinities comparable to EPR3, suggesting a conserved function of these receptor kinases in glycan perception.
Article
Plant Sciences
Ivette Garcia-Soto, Raphael Boussageon, Yareni Marlene Cruz-Farfan, Jesus Daniel Castro-Chilpa, Liz Xochiquetzal Hernandez-Cerezo, Victor Bustos-Zagal, Alfonso Leija-Salas, Georgina Hernandez, Martha Torres, Damien Formey, Pierre-Emmanuel Courty, Daniel Wipf, Mario Serrano, Alexandre Tromas
Summary: ROP3 has been identified as a positive regulator of infection thread and nodule formation in Lotus japonicus, while not affecting AMF root colonization. The expression of CSSP genes was not impacted by ROP3 in the NFS and AMS conditions, but some rhizobial-specific pathway genes showed decreased expression in the NFS.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yanping Wang, Wenqing Zhou, Jiandong Wu, Kailing Xie, Xiaoyu Li
Summary: This study identified the ammonium transporter LjAMT2;2 in Lotus japonicus as an important player in the arbuscular mycorrhizal symbiosis process, facilitating ammonium transfer and promoting nitrogen absorption. Overexpression of LjAMT2;2 increased nitrogen content, alleviating nitrogen stress and enhancing plant growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Mitsutaka Fukudome, Haruka Ishizaki, Yuta Shimokawa, Tomoko Mori, Nahoko Uchi-fukudome, Kamolchanok Umnajkitikorn, Ei-ichi Murakami, Toshiki Uchiumi, Masayoshi Kawaguchi
Summary: This study investigates the role of reactive sulfur species (RSS) produced by cystathionine gamma-lyase (CSE) in root nodule symbiosis. The CSE mutation in Mesorhizobium loti leads to decreased production of hydrogen sulfide and other RSS. The CSE mutation does not affect early stages of symbiosis but reduces nitrogenase activity and induces early senescence of nodules.
MICROBES AND ENVIRONMENTS
(2023)
Article
Biochemistry & Molecular Biology
Longlong Wang, Jianjun Liang, Yu Zhou, Tao Tian, Baoli Zhang, Deqiang Duanmu
Summary: In this study, 13 carbonic anhydrase (CA) genes were identified in the model legume Lotus japonicus, with three of them further characterized for their expression patterns during nodule development. Knockout experiments using CRISPR/Cas9 technology showed that the knocked-out genes were not essential for nitrogen fixation under normal symbiotic conditions. The diverse expression patterns and distributions of these genes suggest their potential functions in symbiotic nitrogen fixation, awaiting further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yvette Hill, Elena Colombi, Emma Bonello, Timothy Haskett, Joshua Ramsay, Graham O'Hara, Jason Terpolilli
Summary: Rhizobia are important soil bacteria capable of forming N-2-fixing symbioses with legumes, but introduction with exotic plants may lead to transfer and evolution of ineffective rhizobia. In Australia, chickpeas inoculated with Mesorhizobium ciceri CC1192 harbor a large symbiosis ICE in their genome, showing potential for environmental transfer and maintaining N-2 fixation efficiency across diverse Mesorhizobium backgrounds.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Jillian M. Petersen, Benedict Yuen
Summary: Nitrogen fixation is a widespread metabolic trait in certain types of microorganisms called diazotrophs. Various organisms have evolved symbioses with diverse diazotrophic bacteria, with enormous economic and ecological benefits. Chemosynthetic nitrogen-fixing symbionts, recently discovered in marine clams, play a crucial role in providing nitrogen in symbiotic relationships.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yong Feng, Ping Wu, Chao Liu, Liwei Peng, Tao Wang, Chao Wang, Qian Tan, Bixuan Li, Yajuan Ou, Hui Zhu, Songli Yuan, Renliang Huang, Gary Stacey, Zhongming Zhang, Yangrong Cao
Summary: The study found that in Lotus japonicus, SymRK is required for rhizobial suppression of plant innate immunity, while LjBAK1 plays a negative role in rhizobial infection. The protein complex formed by SymRK and LjBAK1 serves as an intersection point between rhizobial symbiotic signaling pathways and innate immunity pathways.
Article
Plant Sciences
Meng Liu, Hiromu Kameoka, Akiko Oda, Taro Maeda, Takashi Goto, Koji Yano, Takashi Soyano, Masayoshi Kawaguchi
Summary: Legumes form root nodules in association with rhizobia to overcome nitrogen deficiency. ERN1 is a crucial transcription factor involved in the establishment of root nodule symbiosis, regulating processes such as cell wall remodeling and signal transduction. RNA sequencing revealed 234 genes associated with ERN1, involved in cell wall remodeling, signal transduction, hormone metabolism, and transcription regulation, expanding our understanding of the role of ERN1 in root nodule symbiosis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biology
Kenjiro W. Quides, Yoobeen Lee, Teresa Hur, Hagop S. Atamian
Summary: This research investigates the symbiotic relationship between Lotus japonicus and three rhizobia strains from Mesorhizobium japonicum. It is found that the plant selectively favors the highly beneficial rhizobia, excluding the moderately beneficial and non-beneficial rhizobia. However, when only the moderately beneficial and non-beneficial rhizobia are present, the plant cannot choose the moderately beneficial ones. Further studies on this interaction will help develop highly beneficial rhizobia for increased agricultural productivity.
Article
Biochemistry & Molecular Biology
Akihiro Yamazaki, Kai Battenberg, Yoshikazu Shimoda, Makoto Hayashi
Summary: LysM-like receptor kinases play important roles in both plant immunity and symbiosis, particularly in signal perception and activation of downstream signal cascades. The study revealed that NHL13 interacts with NFR1 and SymRK, suggesting its involvement in both plant immunity and symbiosis.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Review
Plant Sciences
Nadiatul A. Mohd-Radzman, Colleen Drapek
Summary: Plant root architecture is developmentally plastic and forms dedicated cells and organs to host symbionts, such as nitrogen-fixing nodules and myconodules. These symbiotic associations provide different levels of compartmentalisation, allowing the plant to regulate symbiotic interactions. This review discusses the developmental strategy of symbiont compartmentalisation by the plant host and speculates on how spatial confinement mitigates risks associated with root symbiosis.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Yuta Sugiura, Rei Akiyama, Sachiko Tanaka, Koji Yano, Hiromu Kameoka, Shiori Marui, Masanori Saito, Masayoshi Kawaguchi, Kohki Akiyama, Katsuharu Saito
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Plant Sciences
Akira Akamatsu, Miwa Nagae, Yuka Nishimura, Daniela Romero Montero, Satsuki Ninomiya, Mikiko Kojima, Yumiko Takebayashi, Hitoshi Sakakibara, Masayoshi Kawaguchi, Naoya Takeda
Summary: Research shows that gibberellic acid (GA) inhibits root nodule symbiosis by regulating a negative-feedback system called autoregulation of nodulation (AON). GA signaling induces the expression of the symbiotic transcription factor NODULE INCEPTION (NIN), which activates the AON system to regulate nodule formation.
Review
Plant Sciences
Takashi Soyano, Meng Liu, Masayoshi Kawaguchi, Makoto Hayashi
Summary: The formation of root nodules in legumes and actinorhizal plants involves distinct processes compared to lateral root development, with different types of nodules showing variation. Evolution of new organs like root nodules is believed to occur through rearrangement of molecular networks guided by neo-functionalized factors. Evidence suggests that root nodule formation is linked to root or lateral root developmental pathways acquired by the common ancestor of nitrogen-fixing Glade.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Correction
Plant Sciences
Toshio Aoki, Masayoshi Kawaguchi, Haruko Imaizumi-Anraku, Shoichiro Akao, Shin-ichi Ayabe, Tomoyoshi Akashi
JOURNAL OF PLANT RESEARCH
(2021)
Correction
Plant Sciences
Yuji Tokumoto, Kayo Hashimoto, Takashi Soyano, Seishiro Aoki, Wataru Iwasaki, Mai Fukuhara, Tomomi Nakagawa, Kazuhiko Saeki, Jun Yokoyama, Hironori Fujita, Masayoshi Kawaguchi
Summary: A correction to this paper has been published.
JOURNAL OF PLANT RESEARCH
(2021)
Review
Plant Sciences
Nao Okuma, Masayoshi Kawaguchi
Summary: This article discusses the impact of soil nutrient changes on long-distance signaling between the shoot and roots of land plants, highlighting the importance of miR2111 in regulating nodulation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Cuc Thi Nguyen, Katsuharu Saito
Summary: Arbuscular mycorrhizal fungi play a critical role in providing plants with soil nutrients, especially phosphorus. The study reveals that phosphorus transfer in arbuscules may involve polyphosphate in fungal cell walls and apoplastic phosphatases as key players.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Cuc Thi Nguyen, Tatsuhiro Ezawa, Katsuharu Saito
Summary: This study conducted a comprehensive survey of VTC proteins in eight AM fungal genomes and characterized the biochemical properties of the Rhizophagus irregularis VTC4. The results suggest that AM fungal VTC4 not only synthesizes polyP but also regenerates ATP from polyP and ADP.
SOIL SCIENCE AND PLANT NUTRITION
(2022)
Article
Biology
Sachiko Tanaka, Kayo Hashimoto, Yuuki Kobayashi, Koji Yano, Taro Maeda, Hiromu Kameoka, Tatsuhiro Ezawa, Katsuharu Saito, Kohki Akiyama, Masayoshi Kawaguchi
Summary: This study investigates the effects of two plant hormones on the growth and sporulation of the arbuscular mycorrhizal fungus, Rhizophagus clarus. The results demonstrate that these hormones can induce the production of a large number of spores and promote the growth of host plants.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fumika Misawa, Momoyo Ito, Shohei Nosaki, Hanna Nishida, Masahiro Watanabe, Takamasa Suzuki, Kenji Miura, Masayoshi Kawaguchi, Takuya Suzaki
Summary: In this study, researchers discovered that LjNRT2.1 plays a crucial role in the LjNLP1-LjNLP4 signaling pathway by controlling nitrate uptake/transport to regulate nodulation. LjNLP1 and LjNRT2.1 are both important regulators in nodulation. These findings reveal a plant strategy in regulating nitrogen acquisition.
Article
Plant Sciences
Mika Hayashi-Tsugane, Masayoshi Kawaguchi
Summary: The leucine-rich repeat receptor kinase HAR1 mediates signaling pathways that suppress root branching and promote primary root length in response to nitrate supply. These findings are important for understanding the regulation of plant root morphology.
Article
Multidisciplinary Sciences
Takashi Goto, Takashi Soyano, Meng Liu, Tomoko Mori, Masayoshi Kawaguchi
Summary: Nodule development requires spatiotemporal coordination between root epidermis and root cortex. In this study, we found that IAA carboxyl methyltransferase 1 (IAMT1) is transiently induced in wild-type roots at early stages of infection, but shows different expression dynamics in the mutant daphne. Knockdown of one of the IAMT1s, IAMT1a, inhibits nodule development in the root cortex. We also observed an increase in root MeIAA levels with rhizobial infection, and application of MeIAA induces expression of the symbiotic gene NIN in the absence of infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Meng Liu, Hiromu Kameoka, Akiko Oda, Taro Maeda, Takashi Goto, Koji Yano, Takashi Soyano, Masayoshi Kawaguchi
Summary: Legumes form root nodules in association with rhizobia to overcome nitrogen deficiency. ERN1 is a crucial transcription factor involved in the establishment of root nodule symbiosis, regulating processes such as cell wall remodeling and signal transduction. RNA sequencing revealed 234 genes associated with ERN1, involved in cell wall remodeling, signal transduction, hormone metabolism, and transcription regulation, expanding our understanding of the role of ERN1 in root nodule symbiosis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hanna Nishida, Shohei Nosaki, Takamasa Suzuki, Momoyo Ito, Takuya Miyakawa, Mika Nomoto, Yasuomi Tada, Kenji Miura, Masaru Tanokura, Masayoshi Kawaguchi, Takuya Suzaki
Summary: Leguminous plants produce nodules for nitrogen fixation, but halt nodule development when sufficient nitrogen nutrients are present. Understanding how nitrate controls nodulation remains unclear, despite the identification of legume NODULE INCEPTION (NIN)-LIKE PROTEIN (NLP) transcription factors.