Article
Biochemistry & Molecular Biology
Yanis Zekri, Romain Guyot, Frederic Flamant
Summary: We have created a database that integrates RNA-seq and ChIP-seq data to better understand the target genes of thyroid hormone receptors in different cell types. This database serves as a resource for studying the mode of action of thyroid hormone and provides a user-friendly tool to access specific gene information and extract gene lists of interest.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Pengliang Liu, Diyan Li, Jiaman Zhang, Mengnan He, Yan Li, Rui Liu, Mingzhou Li
Summary: The storage of lipids as energy in adipose tissue is conserved, but there are substantial differences in the physiological activities of adipose tissues among species. This study characterized the transcriptional and lipidomic profiles of subcutaneous and visceral adipose tissues across 15 vertebrate species, spanning more than 300 million years of evolution. The data revealed greater variation in AT transcriptome and lipidome among species than within the same species, providing a resource for future studies on functional differences among adipose tissues in vertebrate species.
Article
Endocrinology & Metabolism
Zhen Yuan, Xufang Shen, Hongwei Yan, Jieming Jiang, Binwei Liu, Lei Zhang, Yumeng Wu, Ying Liu, Qi Liu
Summary: The study found that treatment with MET induces masculinization in Takifugu rubripes larvae, while T4 treatment does not feminize them. MET treatment suppresses the expression of foxl2 and cyp19a1a, and induces the expression of dmrt1. T4 treatment temporarily increases cyp19a1a expression in genetic XY gonads, but does not lead to feminization. Further investigation on the effects of higher concentrations of T4 or different concentrations of T3 on sex differentiation is needed.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mehdi Moradi, Mahdi Zhandi, Mohsen Sharafi, Arvand Akbari, Mohammad Jafari Atrabi, Mehdi Totonchi
Summary: This study investigated the causes of retained placenta (RP) in cattle using RNA-seq, hematologic and histologic experiments. The researchers identified differentially expressed genes (DEGs) related to immune system and lipid metabolism pathways in RP cows. They also found that estradiol-17 beta (E2) may be involved in RP development. In addition, histologic experiments showed structural differences in the placentome of RP cows. Complete blood cell (CBC) parameters were not significantly different between the RP and control groups.
Article
Biochemistry & Molecular Biology
Reyna Penailillo, Lara J. Monteiro, Stephanie Acuna-Gallardo, Felipe Garcia, Victoria Velasquez, Paula Correa, Pilar Diaz, Patricia P. Valdebenito, Cristina Navarro, Roberto Romero, Mario Sanchez, Sebastian E. Illanes, Gino Nardocci
Summary: This study aimed to identify potential lncRNA biomarkers for preeclampsia by analyzing the expression of lncRNAs in the placentas and plasma of patients. Six lncRNAs were validated and differentially expressed in preeclampsia placentas, two of which were also detected in maternal plasma, indicating their potential use as biomarkers for the disease.
Article
Biochemistry & Molecular Biology
Konstantinos J. Bogias, Stephen M. Pederson, Shalem Leemaqz, Melanie D. Smith, Dale McAninch, Tanja Jankovic-Karasoulos, Dylan McCullough, Qianhui Wan, Tina Bianco-Miotto, James Breen, Claire T. Roberts
Summary: This study profiles transcript expression profiles in human placental tissue samples and finds that early placental development is mainly regulated by differential transcript expression (DTE) rather than differential gene expression (DGE). Transcript usage is likely a regulatory mechanism in early placentation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Aleksandra Lipka, Jan Pawel Jastrzebski, Lukasz Paukszto, Karol Gustaw Makowczenko, Elzbieta Lopienska-Biernat, Marek Gowkielewicz, Ewa Lepiarczyk, Marta Wiszpolska, Mariusz Krzysztof Majewski, Marta Majewska
Summary: Impaired fetal growth is a major cause of prematurity, stillbirth, and infant mortality. This study investigated non-coding RNAs in the placentas of male and female fetuses with FGR. Dysregulation of lncRNAs was found to affect vasculature in female placentas, while immune processes were more affected in male placentas.
Article
Biochemistry & Molecular Biology
Marie Herrnhold, Isabel Hamp, Oliver Plettenburg, Martin Jastroch, Michaela Keuper
Summary: N-acyl amino acids (NAAs) are endogenous molecules that act as mitochondrial uncouplers in human adipocytes. While NAAs effectively induce uncoupled respiration, high concentrations of NAAs reduce adipocyte functionality and viability. Hence, the suitability of NAAs as anti-obesity agents without further chemical modifications is questionable.
Article
Biochemistry & Molecular Biology
Muhammad Jawaad Atif, Bakht Amin, Muhammad Imran Ghani, Muhammad Ali, Somia Khursheed, Zhihui Cheng
Summary: The study found that garlic plants exhibit different responses to long day and short day in terms of plant hormone levels, with differential gene expression enriching various pathways, particularly those related to plant hormone signaling and photoperiod. The outcomes provide valuable insights into the response mechanisms of garlic to photoperiod.
Article
Endocrinology & Metabolism
Liang Hu, Chao Wu
Summary: Thyroid hormones regulate a variety of functions through THRA and THRB in different cell types, leading to varying responses. Single-cell RNA sequencing technology allows tracking of responsive cell types in tissues, with Tabula Muris Consortium providing data for cell type resolution understanding of thyroid hormone regulation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
Xuan Li, Tao Yu, Muxin Zhai, Yongyuan Wu, Baojing Zhao, Cancan Duan, Huiru Cheng, Han Li, Zhaolian Wei, Yuanyuan Yang, Zhen Yu
Summary: This study found that cadmium exposure reduces placental angiogenesis in preeclampsia by decreasing the expression of PLGF and VEGF and increasing the expression of sFlt1. Cadmium exposure also decreases the expression of thyroid hormone receptor alpha and Dio2. These results suggest that cadmium exposure impairs placental angiogenesis in preeclampsia by disturbing thyroid hormone receptor signaling.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Endocrinology & Metabolism
Yuta Tanizaki, Yuki Shibata, Hongen Zhang, Yun-Bo Shi
Summary: The study demonstrates a close relationship between TR alpha-regulated T3 activation of the cell cycle program and larval epithelial cell death and adult epithelial stem cell development during intestinal remodeling in Xenopus tropicalis metamorphosis.
Article
Plant Sciences
Baike Wang, Juan Wang, Tao Yang, Jinxin Wang, Qi Dai, Fulin Zhang, Rui Xi, Qinghui Yu, Ning Li
Summary: Salt stress is a major limiting factor for tomato growth and fruit quality. The study analyzed the relationship between hormones, transcription factors, and genes in tomatoes under salt stress, and constructed gene regulatory networks. The levels of ABA, SA, and JA increased significantly after salt treatment, while GA levels decreased. The study also identified hormone-related candidate genes associated with salt stress. This research provides valuable insights into the molecular mechanisms of salt tolerance in tomatoes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Endocrinology & Metabolism
Han Shu, Yubing Huang, Wenqian Zhang, Li Ling, Yuanyuan Hua, Zhengai Xiong
Summary: Menopause increases the risk of sarcopenia in females, but the exact pathogenesis remains unclear. Through ovariectomy model and transcriptome sequencing, we identified genes associated with obesity, osteoporosis, and sarcopenia, confirming the importance of estrogen in skeletal muscle function and homeostasis.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Wenfei Sun, Hua Dong, Christian Wolfrum
Summary: The study reveals that local acetate directly inhibits brown adipose tissue thermogenesis, decreases energy expenditure, and down-regulates genes related to the tricarboxylic acid cycle and brown adipocyte formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Michela Palmieri, Teenamol E. Joseph, Charles A. O'Brien, Horacio Gomez-Acevedo, Stavros C. Manolagas, Elena Ambrogini
Summary: The expression of Scarb1 in osteoblasts does not play an important role in bone homeostasis and is not essential for the effects of PC-OxPL on these cells.
Article
Nutrition & Dietetics
Martin J. J. Ronis, Horacio Gomez-Acevedo, Kartik Shankar, Leah Hennings, Neha Sharma, Michael L. Blackburn, Isabelle Miousse, Harry Dawson, Celine Chen, Kelly E. Mercer, Thomas M. Badger
Summary: This study found that soy infant formula does not have estrogenic effects or significantly alter male reproductive development in newborn male piglets, compared to sow milk and milk formula.
Article
Pediatrics
Stephanie P. Gilley, Meghan L. Ruebel, Clark Sims, Ying Zhong, Donald Turner, Renny S. Lan, Lindsay M. Pack, Brian D. Piccolo, Sree Chintapalli, Ann Abraham, Lars Bode, Aline Andres, Kartik Shankar
Summary: Maternal obesity is associated with changes in infant fecal microbiome, short-chain fatty acids (SCFA), and human milk oligosaccharides (HMO). Offspring of women with obesity have lower abundance of SCFA-producing bacteria and lower levels of faecal butyric acid. Overall microbial richness may aid in prediction of elevated adiposity in later infancy.
Article
Biochemistry & Molecular Biology
Jin-Ran Chen, Perry C. Caviness, Haijun Zhao, Beau Belcher, Umesh D. Wankhade, Kartik Shankar, Michael L. Blackburn, Oxana P. Lazarenko
Summary: Studies have shown that maternal chronic poor-quality diet, especially a high fat diet, is associated with reduced bone density and childhood fractures in offspring. The research findings indicate that maternal high fat diet changes histone methylation and acetylation epigenetic marks, which regulate the expression of genes controlling osteoblastogenesis.
Article
Microbiology
Minghua Tang, Nicholas E. Weaver, Daniel N. Frank, Diana Ir, Charles E. Robertson, Jennifer F. Kemp, Jamie Westcott, Kartik Shankar, Ana L. Garces, Lester Figueroa, Antoinette K. Tshefu, Adrien L. Lokangaka, Shivaprasad S. Goudar, Manjunath Somannavar, Sumera Aziz, Sarah Saleem, Elizabeth M. McClure, K. Michael Hambidge, Audrey E. Hendricks, Nancy F. Krebs
Summary: The objective of this study was to characterize the changes in gut microbiota during pregnancy and to determine the effects of nutritional intervention on gut microbiota in women from sub-Saharan Africa, South Asia, and Central America. The results showed that the composition of gut microbial community changed significantly during pregnancy, with a decrease in alpha-diversity. Nutritional intervention did not have a significant impact on inflammatory biomarkers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Catherine C. Cohen, Dana Dabelea, Gregory Michelotti, Lu Tang, Kartik Shankar, Michael Goran, Wei Perng
Summary: This study aimed to assess the intermediary metabolic alterations between sugar-sweetened beverage intake and cardiometabolic risk factors in youth. The researchers identified a panel of lipid metabolites that may serve as biomarkers linking sugar-sweetened beverage intake to dyslipidemia risk in youth.
Article
Physiology
Harrison D. Stierwalt, E. Matthew Morris, Adrianna Maurer, Udayan Apte, Kathryn Phillips, Tiangang Li, Grace M. E. Meers, Lauren G. Koch, Steven L. Britton, Greg Graf, R. Scott Rector, Kelly Mercer, Kartik Shankar, John P. Thyfault
Summary: The study found that high aerobic capacity and exercise are associated with upregulation of bile acid synthesis and greater fecal excretion of cholesterol and bile acid, which may contribute to the protection against hepatic steatosis in rodents.
PHYSIOLOGICAL REPORTS
(2022)
Article
Nutrition & Dietetics
Catherine C. Cohen, Kylie K. Harrall, Stephanie P. Gilley, Wei Perng, Katherine A. Sauder, Ann Scherzinger, Kartik Shankar, Shikha S. Sundaram, Deborah H. Glueck, Dana Dabelea
Summary: This study found that children with higher hepatic fat in early childhood had smaller birth weight and faster adiposity accretion in the first 5 years. This suggests that promoting healthy body composition early in life may be critical for preventing pediatric NAFLD.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Henry A. Paz, Anna-Claire Pilkington, Ying Zhong, Sree Chintapalli, James Sikes, Renny S. Lan, Kartik Shankar, Umesh D. Wankhade
Summary: The gut microbiota plays a critical role in energy homeostasis and its dysbiosis is associated with obesity. Maternal high-fat diet and beta-adrenergic stimuli independently alter the gut microbiota, but their combined regulation is not clear. This study investigated the combined effect of these factors on offspring microbiota and found that maternal high-fat diet altered the offspring's response to beta-adrenergic stimuli, as well as microbial composition and function. Additionally, exposure to different temperatures also affected bacterial function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Whitney E. Jackson, Alyson Kaplan, Jessica L. Saben, Michael S. Kriss, Jaime Cisek, Benjamin Samstein, AnnMarie Liapakis, Anjana A. Pillai, Robert S. Brown, Elizabeth A. Pomfret
Summary: Living donor liver transplantation (LDLT) is an effective solution for the organ shortage in the US, but there is limited understanding of the medical evaluation practices for living liver donors. A survey was conducted on all 53 active LDLT transplant programs in the US, revealing consensus on certain criteria such as minimum age and exclusion of obese donors, while variations existed in areas like acceptance of donors with diabetes mellitus. The findings highlight the need for data generation to develop evidence-based guidelines for the evaluation and risk assessment of living liver donors.
LIVER TRANSPLANTATION
(2023)
Article
Surgery
Premal Trivedi, Jessica L. Saben, Lisa Liu, John S. Malamon, Elizabeth Pomfret, Thomas Pshak
Summary: Biliary anastomotic stricture (BAS) is a common complication of liver transplantation with limited effective treatment options. In this case series, a novel percutaneous laser stricturotomy treatment was shown to be safe and effective in 5 post-transplant patients with refractory BAS. The laser excision and promotion of biliary re-epithelization yielded a 100% technical success rate and no recurrences at a mean follow-up time of 22 months.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Review
Surgery
Toshitaka Sugawara, Salvador Rodriguez Franco, Jun Ishida, Avash Kalra, Jessica L. Saben, Karla Navarrete Galvez, Michael J. Kirsch, Mohammed H. Al-Musawi, Bruce Kaplan, Elizabeth A. Pomfret, Richard D. Schulick, Marco Del Chiaro
Summary: This study found that the progression of intraductal papillary mucinous neoplasm (IPMN) of the pancreas in solid organ transplantation recipients does not significantly increase compared to the general nontransplant population. However, further studies are needed for confirmation due to limited literature.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Gastroenterology & Hepatology
Hunter B. Moore, Jessica Saben, Ivan Rodriguez, Yanik J. Bababekov, James J. Pomposelli, Dor Yoeli, Tanner Ferrell, Megan A. Adams, Thomas J. Pshak, Bruce Kaplan, Elizabeth A. Pomfret, Trevor L. Nydam
Summary: Perioperative dysfunction of the fibrinolytic system may contribute to adverse outcomes in liver transplant recipients. This study examined the association between fibrinolysis resistance (FR), measured by tissue plasminogen activator thrombelastography on postoperative day one, and early allograft dysfunction (EAD). The results showed that FR on POD-1 was significantly associated with increased risk of EAD and decreased graft survival time. This suggests that postoperative viscoelastic testing could be a useful tool in identifying liver transplant recipients at risk for EAD, particularly in those receiving donation after circulatory determination of death grafts.
LIVER TRANSPLANTATION
(2023)
Article
Surgery
John S. Malamon, Bruce Kaplan, Whitney E. Jackson, Jessica L. Saben, Jesse D. Schold, Elizabeth A. Pomfret, James J. Pomposelli
Summary: This study challenges the current perception of the timing and benefits of DDLT in liver transplantation. The authors found that even at a low MELD-Na score of 12, DDLT provides a significant 1-year survival advantage compared to remaining on the waitlist, with a median estimated life years saved of over 9 years. Furthermore, as MELD-Na scores increase, the time-to-equal risk and time-to-equal survival decrease exponentially.
INTERNATIONAL JOURNAL OF SURGERY
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Alyson Kaplan, Jessica Saben, Michael Kriss, AnnMarie Liapakis, Anjana A. Pillai, Robert S. Brown, Elizabeth Pomfret, Whitney E. Jackson
