Article
Medicine, Research & Experimental
Huimin Shi, Renyu Kong, Xu Miao, Lingshan Gou, Xin Yin, Yuning Ding, Xiliang Cao, Qingyong Meng, Maosheng Gu, Feng Suo
Summary: Reduced levels of PPP1R3G may contribute to the progression of pre-eclampsia by suppressing trophoblast invasion and migration and targeting the Akt/MMP-9 signaling pathway.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Ruby Dhar, Sunil Singh, Indrani Mukherjee, Karthikeyan Pethusamy, Kakali Purkayastha, Prasenjit Das, Jai Bhagwan Sharma, Radhey Shyam Sharma, Subhradip Karmakar
Summary: Implantation in humans involves apposition, adhesion, and invasion of the developing blastocyst into the receptive maternal endometrium. Research has identified key factors in this process, but gaps in our understanding remain. Early pregnancy complications often occur during implantation, when the endometrium is most receptive to the blastocyst.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2021)
Review
Biochemistry & Molecular Biology
Darina Bacenkova, Marianna Trebunova, Dasa Cizkova, Radovan Hudak, Erik Dosedla, Alena Findrik-Balogova, Jozef Zivcak
Summary: The article discusses the complex process of placental implantation and development, as well as current technologies used to study the behavior of placental cells. Regulation by transcription factors leads to differentiation of trophoblast cells and formation of distinct populations. The 3D in vitro model is crucial for studying placental physiology and modeling placental diseases.
Article
Multidisciplinary Sciences
Kaela M. Varberg, Khursheed Iqbal, Masanaga Muto, Mikaela E. Simon, Regan L. Scott, Keisuke Kozai, Ruhul H. Choudhury, John D. Aplin, Rebecca Biswell, Margaret Gibson, Hiroaki Okae, Takahiro Arima, Jay L. Vivian, Elin Grundberg, Michael J. Soares
Summary: Invasive trophoblast cells play a crucial role in placental development and pregnancy outcomes. ASCL2 is identified as a key regulator of invasive trophoblast cell lineage development in humans and rats. ASCL2 deficiency impairs EVT cell differentiation and impacts placental morphogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell Biology
Noritaka Tanaka, Takeharu Sakamoto
Summary: MT1-MMP is a member of the metalloproteinase family, which degrades extracellular matrix components directly and can indirectly degrade type IV collagen through the activation of MMP-2 with TIMP-2. MT1-MMP is initially expressed as an inactive form in the ER and is activated through truncation processing by furin. Upon trafficking from the ER to the cell surface membrane, MT1-MMP exhibits proteolytic activities on surrounding molecules.
Article
Biochemistry & Molecular Biology
Reyna Penailillo, Stephanie Acuna-Gallardo, Felipe Garcia, Lara J. Monteiro, Gino Nardocci, Mahesh A. Choolani, Matthew W. Kemp, Roberto Romero, Sebastian E. Illanes
Summary: This study investigated the proliferation, migration, and impact on trophoblast invasion capacity of MenSCs under different physiological conditions. The findings suggest a potential role for MenSCs in the implantation process, implying a communication between maternal MenSCs and invading trophoblast cells that could be implicated in the etiology of PE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Jan E. Strobech, Pietro Giuriatti, Janine T. Erler
Summary: This review focuses on the role of neutrophils in regulating the extracellular matrix and how neutrophil-secreted proteases promote cancer metastasis.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Reproductive Biology
Yunxiang Li, Yuguang Chen, Wenping Wu, Na Li, Jinlian Hua
Summary: Metalloproteinases, including matrix metalloproteinases and disintegrin metalloproteinases, play important roles in the degradation and reconstruction of the extracellular matrix (ECM). They are associated with tissue development and disease. Although their relationship with male reproduction is clear, the underlying mechanism is still unclear.
Review
Biochemistry & Molecular Biology
Il-Sup Kim, Woong-Suk Yang, Cheorl-Ho Kim
Summary: MMPs are enzymes that play a key role in promoting cell migration by degrading the extracellular matrix (ECM). They are upregulated in cancers and inflamed regions. TIMPs are highly specific inhibitors of MMPs, regulating their activity and potentially playing a role in inhibiting MMP-dependent diseases.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Ecology
Nour Al Haj Baddar, Nataliya Timoshevskaya, Jeramiah J. Smith, Houfu Guo, S. Randal Voss
Summary: This study identified 28 MMPs in salamanders, with 9 MMP paralogs unique to axolotls and other salamanders. These novel salamander MMPs are all of the secreted type, suggesting a unique expansion within salamanders.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Yuyin Yi, Hua Zhu, Christian Klausen, Peter C. K. Leung
Summary: The study identifies SOX4 as a direct target gene induced by BMP2 in trophoblasts during early placental development. SOX4 plays a pro-invasive role in human trophoblasts and contributes to BMP2-enhanced trophoblast invasion by regulating genes associated with cell migration and extracellular matrix organization. Furthermore, SOX4 mediates BMP2-induced trophoblast invasion through binding to regulatory elements like enhancers.
Article
Biochemical Research Methods
Yong Pu, Jeremy Gingrich, Almudena Veiga-Lopez
Summary: Placental trophoblast cell invasion into the maternal uterus is a crucial and complex event in the formation of the maternal-fetal interface. A 3D PDMS microfluidic platform has been developed to accurately evaluate trophoblast invasion, allowing for real-time monitoring, imaging, and collection of invasive cells. This platform provides insights into pathways regulating trophoblast invasion and is useful for toxicological screening.
Article
Food Science & Technology
Limei Yang, Le Hu, Hongyu Tang, Xuemei Chen, Xueqing Liu, Yue Zhang, Yixian Wen, Yongxiu Yang, Yanqing Geng
Summary: Ti3C2 MXene exposure impairs villus explant extension ability and trophoblast cell functions by inhibiting cell migration and invasion abilities, and enhancing autophagy. Inhibition of autophagy may be a potential therapeutic strategy for alleviating nanoparticle-induced placental toxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Chemistry, Medicinal
Defeng Xu, Caihong Li, Mouming Zhao
Summary: The extract from Theragra chalcogramma (TCH) can improve the barrier functions of photoaged skin, promote the deposition of extracellular matrix (ECM) components, and inhibit the activity of MMP-1 in photoaging skin. It also restores the impaired structures in the skin and enhances the expression levels of elastin and fibrillin-1.
Article
Cell Biology
Lanlan Fang, Yibo Gao, Zhen Wang, Yuxi Li, Yang Yan, Ze Wu, Jung-Chien Cheng, Ying-Pu Sun
Summary: This study reveals that downregulation of EGF can lead to poor trophoblast cell invasion by increasing KISS1 expression, which subsequently contributes to the pathogenesis of preeclampsia.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Reproductive Biology
Hanns Helmer, Leila Saleh, Ljubomir Petricevic, Martin Knoefler, Torsten M. Reinheimer
BIOLOGY OF REPRODUCTION
(2020)
Article
Multidisciplinary Sciences
Gudrun Meinhardt, Sandra Haider, Victoria Kunihs, Leila Saleh, Juergen Pollheimer, Christian Fiala, Szabolcs Hetey, Zsofia Feher, Andras Szilagyi, Nandor Gabor Than, Martin Knoefler
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Oncology
Hong Nhung Vu, Ramile Dilshat, Valerie Fock, Eirikur Steingrimsson
Summary: The proteins MITF, TFEB, TFE3, and TFEC are closely related and regulated by the same signaling pathways, but the presence of multiple isoforms and post-translational modifications make it challenging to index amino acid residues across different proteins within the MiT-TFE family.
PIGMENT CELL & MELANOMA RESEARCH
(2021)
Article
Reproductive Biology
Elisa T. Zhang, Roberta L. Hannibal, Keyla M. Badillo Rivera, Janet H. T. Song, Kelly McGowan, Xiaowei Zhu, Gudrun Meinhardt, Martin Knoefler, Juergen Pollheimer, Alexander E. Urban, Ann K. Folkins, Deirdre J. Lyell, Julie C. Baker
Summary: The study identified misregulated gene expression patterns in tissues from placenta previa and percreta compared with control cases, with upregulated genes predominantly marking extravillous trophoblasts. PRG2 and AQPEP protein levels were found to be upregulated in both conditions, with AQPEP restricted to trophoblasts and PRG2 mislocalized throughout the fetal membranes. This suggests a signature of previa and PAS in the extraembryonic tissues surrounding the conceptus, reflecting increased trophoblast invasiveness.
BIOLOGY OF REPRODUCTION
(2021)
Article
Immunology
R. Lindau, S. Vondra, J. Spreckels, M. Solders, J. Svensson-Arvelund, G. Berg, J. Pollheimer, H. Kaipe, M. C. Jenmalm, J. Ernerudh
Summary: The study found that maternal decidual stromal cells can induce specific types of macrophages and Treg cells, thereby influencing the tolerogenic environment at the foetal-maternal interface during pregnancy.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Engineering, Biomedical
Abigail J. Deloria, Sandra Haider, Bianca Dietrich, Victoria Kunihs, Simon Oberhofer, Martin Knofler, Rainer Leitgeb, Mengyang Liu, Wolfgang Drexler, Richard Haindl
Summary: The study demonstrates the use of 3D optical coherence tomography (OCT) for analyzing human placenta organoids without sample preparation. Results show that 3D OCT enables detailed monitoring of placenta organoids, visualizing and quantifying internal structures in situ to assess differentiation status and aid advancement in the field.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Article
Biology
Elisabeth Gludovacz, Kornelia Schuetzenberger, Marlene Resch, Katharina Tillmann, Karin Petroczi, Markus Schosserer, Sigrid Vondra, Serhii Vakal, Gerald Klanert, Jurgen Pollheimer, Tiina A. Salminen, Bernd Jilma, Nicole Borth, Thomas Boehm
Summary: By mutating the heparin-binding motif of hDAO, the plasma clearance rate was successfully reduced in rodents, showing potential for treating diseases characterized by high plasma histamine concentrations.
Review
Biochemistry & Molecular Biology
Bianca Dietrich, Sandra Haider, Gudrun Meinhardt, Juergen Pollheimer, Martin Knoefler
Summary: This article discusses two important signaling pathways, WNT and NOTCH, in the development of human placenta and trophoblasts. The WNT pathway is necessary for the expansion of trophoblast progenitors and stem cells, while NOTCH1 is required for the proliferation and survival of extravillous trophoblast precursors.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Sandra Haider, Andreas Ian Lackner, Bianca Dietrich, Victoria Kunihs, Peter Haslinger, Gudrun Meinhardt, Theresa Maxian, Leila Saleh, Christian Fiala, Juergen Pollheimer, Paulina A. Latos, Martin Knoefler
Summary: Comparative gene expression analysis revealed the importance of transforming growth factor-beta (TGF-beta) signaling in the development of extravillous trophoblasts (EVTs), particularly in the differentiation of placental EVTs into decidual EVTs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Soma Ray, Abhik Saha, Ananya Ghosh, Namrata Roy, Ram P. Kumar, Gudrun Meinhardt, Abhirup Mukerjee, Sumedha Gunewardena, Rajnish Kumar, Martin Knoefler, Soumen Paul
Summary: WWTR1 is identified as a master regulator of trophoblast fate choice during human placentation, promoting CTB self-renewal and EVT differentiation while preventing STB induction. The fine-tuning of trophoblast fate by WWTR1 is achieved through direct regulation of WNT signaling components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Sigrid Vondra, Anna-Lena Hobler, Andreas Ian Lackner, Johanna Raffetseder, Zala Nikita Mihalic, Andrea Vogel, Leila Saleh, Victoria Kunihs, Peter Haslinger, Markus Wahrmann, Heinrich Husslein, Raimund Oberle, Julia Kargl, Sandra Haider, Paulina Latos, Gernot Schabbauer, Martin Knofler, Jan Ernerudh, Jurgen Pollheimer
Summary: During pregnancy, different macrophage populations are found in the decidua basalis (decB) and decidua parietalis (decP), with decBAMs being CD11chi and efficient inducers of Tregs, while decPAMs exhibit a dendritic cell-like phenotype, enhanced phagocytosis, and the ability to activate T cells. Strikingly, EVT-conditioned media can convert decPAMs into a decBAM phenotype, highlighting the critical role of extravillous trophoblasts (EVTs) in the induction of decB-associated macrophage polarization.
Article
Developmental Biology
Bianca Dietrich, Victoria Kunihs, Juergen Pollheimer, Martin Knoefler, Sandra Haider
Summary: 3D trophoblast organoids (TB-ORG) are a reliable model for studying extravillous trophoblast (EVT) lineage formation and differentiation. However, alternative cell sources are needed due to limited access to first trimester placentae. In this study, we tested whether commonly used trophoblastic cell lines, ACH-3P, HTR-8/SVneo, and SWAN-71, could self-organize into organoids and differentiate into EVT. Only ACH-3P formed organoids resembling TB-ORG architectures, and induction of EVT differentiation resulted in the formation of HLA-Gpos areas. Therefore, ACH-3P-ORGs provide another model for studying controlled EVT lineage formation and differentiation.
Article
Developmental Biology
Bianca Dietrich, Victoria Kunihs, Andreas I. Lackner, Gudrun Meinhardt, Bon-Kyoung Koo, Jurgen Pollheimer, Sandra Haider, Martin Knoefler
Summary: This study reveals that autocrine NOTCH3 signaling controls human placental development by promoting self-renewal of cytotrophoblast progenitors.