期刊
PIGMENT CELL & MELANOMA RESEARCH
卷 25, 期 6, 页码 773-782出版社
WILEY
DOI: 10.1111/pcmr.12005
关键词
melanoma; Hic5 protein; mouse; RhoA GTP-binding protein; neoplasm metastasis
资金
- Ministry of Education, Science, Sports and Culture of Japan
- Grants-in-Aid for Scientific Research [23592647] Funding Source: KAKEN
Hic-5 is a shuttling protein between the cell membrane and the nucleus which functions as a focal adhesion adaptor protein and a nuclear receptor coactivator. Although several studies have shown its involvement in other types of cancer, the role of Hic-5 in melanoma is unknown. Herein, we show for the first time that Hic-5 is expressed in B16-F1 murine melanoma cells. To determine its function in melanoma cells, we used shRNA-mediated RNA interference and established stable clones with down-regulated Hic-5 expression. These clones had impaired growth and metastatic potential compared with controls in vivo, which correlated with decreased proliferation, migration and invasion in vitro. Moreover, silencing of Hic-5 expression in B16-F1 activated RhoA with an amoeboid phenotypic change, indicating that Hic-5 is a key regulator of B16-F1 metastasis in the context of Rho-dependent motility. These results provide new evidence that Hic-5 is a possible molecular target for treatment of melanoma.
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