Article
Oncology
Daniela Planska, Vratislav Horak
Summary: Melanoma is a skin cancer with high immunogenicity and partial spontaneous regression, for which effective therapy is lacking. Animal melanoma models are essential for understanding the immunological processes during spontaneous regression. Flow cytometry analysis revealed that cytotoxic T cells were the most abundant immune cells in MeLiM melanomas of animals aged 10-32 weeks, potentially causing the destruction of melanoma cells. Furthermore, the development of a specific (adaptive) immune response represented mainly by cytotoxic T cells seems crucial for the successful spontaneous regression of porcine melanoma.
ANTICANCER RESEARCH
(2023)
Article
Food Science & Technology
Lijin Duan, Shasha Zhang, Yingxia Yang, Qian Wang, Qingkuo Lan, Yong Wang, Wentao Xu, Wujun Jin, Liang Li, Rui Chen
Summary: This study introduces a novel GMO testing method, "SSH-seq", based on suppressive subtractive hybridization and next-generation sequencing, which can be used to detect unknown GMOs and has shown promising results in experiments. Although the method may be influenced by multiple factors, further optimizations could make SSH-seq a powerful tool in the field of GMO testing.
Article
Biochemistry & Molecular Biology
Hsin-Hung Lin, Kuan-Hung Lin, Kuan-Fu Wu, Yu-Chi Chen
Summary: IbACP, a peptide from sweet potato leaves, affects ROS accumulation and defense gene expression in sweet potato plants. Enhanced POD activity by IbACP treatment suggests a potential mechanism for scavenging H2O2. Genes related to IbACP were classified into various categories, indicating its importance in genetic regulation in sweet potato.
Article
Biochemistry & Molecular Biology
Furong Zeng, Yayun Li, Yu Meng, Huiyan Sun, Yi He, Mingzhu Yin, Xiang Chen, Guangtong Deng
Summary: Combining BET inhibitors with sunitinib showed synergistic antitumor effects in melanoma cells by inducing apoptosis and cell cycle arrest. Mechanistically, BET inhibitors sensitized melanoma cells to sunitinib by inhibiting GDF15 expression, which is transcriptionally regulated by BRD4 or indirectly by the BRD4/IL6/STAT3 axis. Xenograft assays demonstrated that the combination of BET inhibitors with sunitinib suppressed melanoma growth in vivo.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Review
Microbiology
Wenxian Chen, Weijun Wang, Xinyan Wang, Zhaoyao Li, Keke Wu, Xiaowen Li, Yuwan Li, Lin Yi, Mingqiu Zhao, Hongxing Ding, Shuangqi Fan, Jinding Chen
Summary: This paper summarizes various nucleic acid detection methods for vesicular disease pathogens in swine, including traditional RT-PCR and real-time RT-PCR methods, as well as recent developments in isothermal amplification methods and luminex technology. Additionally, the CRISPR-Cas diagnostic system is introduced as an emerging nucleic acid detection technology.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Ciara Tyrrell, Thi Thuy Do, Robert J. Leigh, Catherine M. Burgess, Fiona P. Brennan, Fiona Walsh
Summary: Land spreading of animal manure is a crucial process in agriculture, but the potential of grass phyllosphere as a reservoir of antimicrobial resistance (AMR) remains unknown. A field study was conducted to assess the impact of bovine, swine, and poultry manure application on the grass phyllosphere and soil microbiome and resistome. The results showed that manure treatment introduced clinically important antimicrobial resistance genes into grass and soil, and the temporal analysis indicated similar patterns across different manure types. The study highlights the role of grass phyllosphere as an understudied sink of AMR.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agriculture, Dairy & Animal Science
Yufang Liu, Guiling Cao, Yujing Xie, Mingxing Chu
Summary: The study aimed to identify genes related to precocious puberty in goats by SSH, using pituitary tissues from goats at different growth stages. DEGs were annotated and enriched in GO terms related to ribosome structure, translation, and GTP binding, as well as in KEGG pathways related to Jak-STAT signaling and oocyte meiosis. Candidate genes associated with precocious puberty were screened from the SSH libraries, providing valuable insights for further research into goat reproductive biology.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Song Xiang-peng, Xia Ying-ju, Xu Lu, Zhao Jun-jie, Wang Zhen, Zhao Qi-zu, Liu Ye-bing, Zhang Qian-yi, Wang Qin
Summary: With the implementation of the C-strain vaccine, classical swine fever (CSF) has been controlled in China, while African swine fever (ASF) and atypical porcine pestivirus (APPV) still pose challenges to the pig industry. It is crucial to develop a reliable method for simultaneous detection and differential diagnosis of these diseases. In this study, a multiplex real-time PCR assay was established, which showed high sensitivity and specificity for the detection of CSFV, ASFV, and APPV.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Veterinary Sciences
Dan Yin, Renhao Geng, Hui Lv, Chunhui Bao, Hongxia Shao, Jianqiang Ye, Kun Qian, Aijian Qin
Summary: The real-time PCR assay targeting the A137R gene showed better performance in detecting ASFV from clinical samples compared to the OIE-recommended TaqMan PCR, indicating its potential application in ASF control and eradication programs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Hyun Jin Hwang, Yun Seong Choi, Kyungyoung Song, Maciej Frant, Jeong Hee Kim
Summary: A fast and quantitative molecular detection method of African swine fever virus (ASFV) is presented in this study. The method can detect all genotypes of ASFV based on the major capsid protein p72, with high sensitivity and specificity.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Zoology
Yufang Liu, Guiling Cao, Yujing Xie, Mingxing Chu
Summary: In order to explore the molecular mechanism of puberty effects on goat reproduction, goats at different growth stages from two breeds were divided into three groups and differentially expressed genes related to puberty were screened. A total of 184 differentially expressed genes were identified, and pathway analysis revealed the involvement of various signaling pathways in puberty development. The CYP11A1 gene was predicted to regulate the expression of prolificacy-associated markers in goat ovaries, thus affecting puberty development and improving goat reproduction.
PAKISTAN JOURNAL OF ZOOLOGY
(2023)
Article
Virology
Viviane Saporiti, Laura Valls, Jaime Maldonado, Monica Perez, Florencia Correa-Fiz, Joaquim Segales, Marina Sibila
Summary: PCV-3 was detected in 33.9% of reproductive failure cases, with 16 cases where it was the sole pathogen found. It was found in mild histopathologic lesions in multiple tissues, emphasizing its potential role as a causative agent in swine reproductive failure.
Article
Virology
Jin-Hui Zhu, Gaurav Rawal, Ethan Aljets, Wannarat Yim-Im, Yong-Le Yang, Yao-Wei Huang, Karen Krueger, Phillip Gauger, Rodger Main, Jianqiang Zhang
Summary: A 5-plex real-time RT-PCR method was developed for simultaneous detection and differentiation of four swine enteric coronaviruses. The method had high specificity, sensitivity, and repeatability. SADS-CoV was not detected in the tested samples, but PEDV was frequently detected.
Article
Infectious Diseases
Xiangdong Li, Yongxin Hu, Penggang Liu, Zhenbang Zhu, Paorao Liu, Changhai Chen, Xiaodong Wu
Summary: The established duplex real-time PCR method in this study provides a powerful tool for rapid detection and differentiation between genotypes I and II ASFVs, which is of great importance for the effective control of ASFV.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Veterinary Sciences
Suresh V. Kuchipudi, Meera Surendran Nair, Michele Yon, Abhinay Gontu, Ruth H. Nissly, Rhiannon Barry, Denver Greenawalt, Traci Pierre, Lingling Li, Nagaraja Thirumalapura, Deepanker Tewari, Bhushan Jayarao
Summary: A series of high mortality outbreaks caused by virulent strains of Streptococcus equi subspecies zooepidemicus affected swine herds in the USA and Canada in 2019, requiring rapid and accurate diagnosis. A novel probe-based real-time PCR targeting a conserved region of the SzM gene was developed, showing high sensitivity and specificity for virulent swine isolates without cross reactivity with avirulent strains or other pathogens. The PCR assay demonstrated 100% sensitivity and specificity compared to traditional bacteriological culture methods when tested with clinical samples.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Medicine, Research & Experimental
Arthur Gautron, Laura Bachelot, Marc Aubry, Delphine Leclerc, Anais M. Quemener, Sebastien Corre, Florian Rambow, Anais Paris, Nina Tardif, Heloise M. Leclair, Oskar Marin-Bejar, Cedric Coulouarn, Jean-Christophe Marine, Marie-Dominique Galibert, David Gilot
Summary: Genetic drivers of melanoma development and therapy resistance have been identified, but non-genetic mechanisms are less understood. Through in vivo CRISPR screens, SMAD3, BIRC3, and SLC9A5 were found to play key roles in BRAFi resistance. Upregulation of SMAD3 was linked to resistance prediction and chemical inhibition of SMAD3 showed promise in targeting persister cells associated with melanoma.
EMBO MOLECULAR MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Dennis Pedri, Panagiotis Karras, Ewout Landeloos, Jean-Christophe Marine, Florian Rambow
Summary: The review discusses the emergence of a process similar to EMT in melanoma, as well as how comparative analysis with EMT state(s) in epithelial cancers can provide clues for identifying clinically relevant biomarkers for prognosis and new therapeutic strategies.
Article
Biology
Zackie Aktary, Alejandro Conde-Perez, Florian Rambow, Mathilde Di Marco, Francois Amblard, Ilse Hurbain, Graca Raposo, Cedric Delevoye, Sylvie Coscoy, Lionel Larue
Summary: Dynlt3 plays a crucial role in melanosome transport, maturation, and transfer to keratinocytes, essential for proper skin pigmentation. The Wnt/beta catenin signaling pathway regulates Dynlt3 levels, contributing to the control of melanocyte transport and skin pigmentation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Oncology
Oskar Marin-Bejar, Aljosja Rogiers, Michael Dewaele, Julia Femel, Panagiotis Karras, Joanna Pozniak, Greet Bervoets, Nina Van Raemdonck, Dennis Pedri, Toon Swings, Jonas Demeulemeester, Sara Vander Borght, Stefan Lehnert, Francesca Bosisio, Joost J. van den Oord, Isabelle Vanden Bempt, Diether Lambrechts, Thierry Voet, Oliver Bechter, Helen Rizos, Mitchell P. Levesque, Eleonora Leucci, Amanda W. Lund, Florian Rambow, Jean-Christophe Marine
Summary: Therapy resistance in melanoma can arise from genetic or nongenetic mechanisms, with the emergence of neural crest stem cells in minimal residual disease playing a role in the development of nongenetic resistance. Ablation of these cells through focal adhesion kinase inhibition delays tumor relapse and reveals that ultimately resistant tumors exhibit genetic alterations that increase sensitivity to specific inhibitors.
Article
Cell Biology
Ioannis I. Verginadis, Harris Avgousti, James Monslow, Giorgos Skoufos, Frank Chinga, Kyle Kim, Nektaria Maria Leli, Ilias V. Karagounis, Brett I. Bell, Anastasia Velalopoulou, Carlo Salas Salinas, Victoria S. Wu, Yang Li, Jiangbin Ye, David A. Scott, Andrei L. Osterman, Arjun Sengupta, Aalim Weljie, Menggui Huang, Duo Zhang, Yi Fan, Enrico Radaelli, John W. Tobias, Florian Rambow, Panagiotis Karras, Jean-Christophe Marine, Xiaowei Xu, Artemis G. Hatzigeorgiou, Sandra Ryeom, J. Alan Diehl, Serge Y. Fuchs, Ellen Pure, Constantinos Koumenis
Summary: Bidirectional signalling between the tumour and stroma plays a crucial role in shaping tumour aggressiveness and metastasis. ATF4, a major effector of the Integrated Stress Response, regulates collagen biosynthesis and deposition in perivascular cancer-associated fibroblasts (CAFs), thereby supporting angiogenesis and progression in melanoma and pancreatic cancer.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Panagiotis Karras, Ignacio Bordeu, Joanna Pozniak, Ada Nowosad, Cecilia Pazzi, Nina Van Raemdonck, Ewout Landeloos, Yannick Van Herck, Dennis Pedri, Greet Bervoets, Samira Makhzami, Jia Hui Khoo, Benjamin Pavie, Jochen Lamote, Oskar Marin-Bejar, Michael Dewaele, Han Liang, Xingju Zhang, Yichao Hua, Jasper Wouters, Robin Browaeys, Gabriele Bergers, Yvan Saeys, Francesca Bosisio, Joost van den Oord, Diether Lambrechts, Anil K. Rustgi, Oliver Bechter, Cedric Blanpain, Benjamin D. Simons, Florian Rambow, Jean-Christophe Marine
Summary: By combining mouse genetics, single-cell and spatial transcriptomics, lineage tracing and quantitative modelling, the researchers provide evidence of a hierarchical model of tumour growth in melanoma and reveal the spatial and temporal diversity and trajectories of melanoma cell states. They show that tumorigenic competence is associated with a spatially localized perivascular niche and that only a fraction of cells are fated to fuel primary tumour growth, while another fraction constitutes a pool of metastatic initiating cells that switch cell identity during dissemination to secondary organs. These findings highlight the importance of targeting microenvironmental cues to interfere with the cancer cell reprogramming activity.
Correction
Multidisciplinary Sciences
Panagiotis Karras, Ignacio Bordeu, Joanna Pozniak, Ada Nowosad, Cecilia Pazzi, Nina Van Raemdonck, Ewout Landeloos, Yannick Van Herck, Dennis Pedri, Greet Bervoets, Samira Makhzami, Jia Hui Khoo, Benjamin Pavie, Jochen Lamote, Oskar Marin-Bejar, Michael Dewaele, Han Liang, Xingju Zhang, Yichao Hua, Jasper Wouters, Robin Browaeys, Gabriele Bergers, Yvan Saeys, Francesca Bosisio, Joost van den Oord, Diether Lambrechts, Anil K. Rustgi, Oliver Bechter, Cedric Blanpain, Benjamin D. Simons, Florian Rambow, Jean-Christophe Marine
Correction
Oncology
Yichao Hua, Gerlanda Vella, Florian Rambow, Elizabeth Allen, Asier Antoranz Martinez, Marie Duhamel, Akira Takeda, Sirpa Jalkanen, Steffie Junius, Ann Smeets, David Nittner, Stefanie Dimmeler, Thomas Hehlgans, Adrian Liston, Francesca Maria Bosisio, Giuseppe Floris, Damya Laoui, Maija Hollmen, Diether Lambrechts, Pascal Merchiers, Jean-Christophe Marine, Susan Schlenner, Gabriele Bergers
Article
Oncology
Yichao Hua, Gerlanda Vella, Florian Rambow, Elizabeth Allen, Asier Antoranz Martinez, Marie Duhamel, Akira Takeda, Sirpa Jalkanen, Steffie Junius, Ann Smeets, David Nittner, Stefanie Dimmeler, Thomas Hehlgans, Adrian Liston, Francesca Maria Bosisio, Giuseppe Floris, Damya Laoui, Maija Hollmen, Diether Lambrechts, Pascal Merchiers, Jean-Christophe Marine, Susan Schlenner, Gabriele Bergers
Summary: The lack of T cell infiltrates hinders effective immunotherapy in cancer, while the formation of tumor-associated tertiary-lymphoid-like structures (TA-TLLSs) is associated with a good prognosis. This study reveals that antiangiogenic immune-modulating therapies can induce the transformation of postcapillary venules into inflamed high-endothelial venules (HEVs) through lymphotoxin/lymphotoxin beta receptor (LT/LTbR) signaling. Tumor HEVs enhance intratumoral lymphocyte influx and promote the differentiation of CD8 T cell progenitors into effector cells, contributing to tumor immunotherapy.
Article
Biochemistry & Molecular Biology
Kenji F. Shoji, Elsa Bayet, Sabrina Leverrier-Penna, Dahiana Le Devedec, Aude Mallavialle, Severine Marionneau-Lambot, Florian Rambow, Raul Perret, Aurelie Joussaume, Roselyne Viel, Alain Fautrel, Amir Khammari, Bruno Constantin, Sophie Tartare-Deckert, Aubin Penna
Summary: Melanoma, a highly aggressive cancer, is driven by aberrant cell motility behaviors and the ability to rapidly metastasize. This study reveals the prominent expression of the plasma membrane TRPV2 calcium channel in melanoma tumors, directly linked to metastatic dissemination. TRPV2 activity confers migratory and invasive potentials in both in vitro and in vivo settings, while silencing TRPV2 in highly metastatic melanoma cells prevents aggressive behavior. The TRPV2 channel, localized at the leading edge of invasive melanoma cells, regulates calcium-mediated activation of calpain and subsequent cleavage of talin, along with F-actin organization. Overexpression of TRPV2 in human melanoma tissues is associated with advanced malignancy and poor prognosis, suggesting its potential as a biomarker. By controlling adhesion and motility, the mechanosensitive TRPV2 channel offers a new therapeutic option for migrastatics in metastatic melanoma treatment.
Article
Oncology
Ali Talebi, Vincent de Laat, Xander Spotbeen, Jonas Dehairs, Florian Rambow, Aljosja Rogiers, Frank Vanderhoydonc, Lara Rizotto, Melanie Planque, Ginevra Doglioni, Sahar Motamedi, David Nittner, Tania Roskams, Patrizia Agostinis, Oliver Bechter, Veerle Boecxstaens, Marjan Garmyn, Marie O'Farrell, Alan Wagman, George Kemble, Eleonora Leucci, Sarah-Maria Fendt, Jean-Christophe Marine, Johannes V. V. Swinnen
Summary: This study identifies the role of FASN in therapy resistance in cancer. By inhibiting FASN and inducing ROS, therapy resistance can be delayed. The combination of MAPK inhibitors, FASN inhibitors, and ROS inducers significantly increases survival in therapy-resistant models.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Multidisciplinary Sciences
Bastian Kruse, Anthony C. Buzzai, Naveen Shridhar, Andreas D. Braun, Susan Gellert, Kristin Knauth, Joanna Pozniak, Johannes Peters, Paulina Dittmann, Miriam Mengoni, Tetje Cornelia van der Sluis, Simon Hoehn, Asier Antoranz, Anna Krone, Yan Fu, Di Yu, Magnus Essand, Robert Geffers, Dimitrios Mougiakakos, Sascha Kahlfuss, Hamid Kashkar, Evelyn Gaffal, Francesca M. Bosisio, Oliver Bechter, Florian Rambow, Jean-Christophe Marine, Wolfgang Kastenmueller, Andreas J. Mueller, Thomas Tueting
Summary: Most clinically applied cancer immunotherapies rely on direct recognition and killing of tumor cells by CD8(+) cytolytic T cells. However, the effectiveness of these strategies is limited by the emergence of MHC-deficient tumor cells and immunosuppressive tumor microenvironment. In this study, researchers have discovered that a small number of CD4(+) T cells can eliminate MHC-deficient tumors that escape CD8(+) T cell targeting. CD4(+) effector T cells cluster at tumor invasive margins and interact with MHC-II(+)CD11c(+) antigen-presenting cells, leading to the activation of tumoricidal myeloid cells. Together, CD4(+) T cells and tumoricidal myeloid cells induce inflammatory cell death and eradicate interferon-unresponsive and MHC-deficient tumors, suggesting a potential strategy to complement current cancer immunotherapies.
Article
Oncology
Luiza Martins Nascentes Melo, Dayana Herrera-Rios, Daniel Hinze, Stefanie Loeffek, Irem Oezel, Roberta Turiello, Juliane Klein, Sonia Leonardelli, Isa-Vanessa Westedt, Yahya Al-Matary, Sara Egea-Rodriguez, Alexandra Brenzel, Maja Bau, Antje Sucker, Eva Hadaschik, Florian Wirsdoerfer, Helmut Hanenberg, Niklas Uhlenbrock, Daniel Rauh, Joanna Pozniak, Florian Rambow, Jean-Christophe Marine, Maike Effern, Nicole Glodde, Dirk Schadendorf, Jadwiga Jablonska, Michael Hoelzel, Iris Helfrich
Summary: This study identified HSD11B1 as a negative feedback mechanism that limits the efficacy of PD-1 blockade, and showed that HSD11B1 inhibitors can enhance the effectiveness of immunotherapy for melanoma. Additionally, high levels of HSD11B1 were associated with poor responses to immune checkpoint inhibitors in patients with advanced melanoma.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biotechnology & Applied Microbiology
Irene Talon, Adrian Janiszewski, Bart Theeuwes, Thomas Lefevre, Juan Song, Greet Bervoets, Lotte Vanheer, Natalie De Geest, Suresh Poovathingal, Ryan Allsop, Jean-Christophe Marine, Florian Rambow, Thierry Voet, Vincent Pasque
Summary: Our study reveals that intrinsic compensatory mechanisms exist in mammalian cells, involving modulation of chromatin accessibility to counteract imbalances in gene dosage between the X chromosome and autosomes caused by evolutionary changes or X chromosome inactivation in vitro. Through genome-wide approaches, allele-specific ATAC-seq, and single-cell RNA-seq, we demonstrate increased chromatin accessibility on the upregulated active X chromosome compared to autosomes in female embryonic fibroblasts and during reprogramming to pluripotency. Additionally, our data show that ZFP42/REX1, a gene associated with pluripotency that evolved specifically in placental mammals, targets multiple X-linked genes, suggesting an evolutionary link between ZFP42/REX1, X chromosome reactivation, and pluripotency.
Article
Oncology
Claudie Bosc, Estelle Saland, Aurelie Bousard, Noemie Gadaud, Marie Sabatier, Guillaume Cognet, Thomas Farge, Emeline Boet, Mathilde Gotanegre, Nesrine Aroua, Pierre-Luc Mouchel, Nathaniel Polley, Clement Larrue, Eleonore Kaphan, Muriel Picard, Ambrine Sahal, Latifa Jarrou, Marie Tosolini, Florian Rambow, Florence Cabon, Nathalie Nicot, Laura Poillet-Perez, Yujue Wang, Xiaoyang Su, Quentin Fovez, Jerome Kluza, Rafael Jose Arguello, Celine Mazzotti, Herve Avet-Loiseau, Francois Vergez, Jerome Tamburini, Jean-Jacques Fournie, Ing S. Tiong, Andrew H. Wei, Tony Kaoma, Jean-Christophe Marine, Christian Recher, Lucille Stuani, Carine Joffre, Jean-Emmanuel Sarry
Summary: Therapy resistance in AML is linked to high mitochondrial oxidative phosphorylation identified by MitoScore. Targeting mitochondrial metabolism can delay AML relapse, highlighting the central role of mitochondrial adaptation in AML therapy.
