Extract of Chaga Mushroom (Inonotus obliquus) stimulates 3t3-l1 adipocyte differentiation

Chaga mushroom (Inonotus obliquus) has long been used as a folk medicine due to its numerous biological functions such as antibacterial, antiallergic, antiinflammatory and antioxidative activities. In the present study, it was found that the I. obliquus hot water extract (IOWE) activated adipogenesis of 3T3-L1 preadipocytes. Even in the absence of adipogenic stimuli by insulin, the IOWE strongly induced adipogenesis of 3T3-L1 preadipocytes. The major constituent of IOWE was glucose-rich polysaccharides with a molecular mass of 149 kDa. IOWE enhanced the differentiation of 3T3-L1 preadipocytes, increasing TG (triacylglycerol) accumulation that is critical for acquisition of the adipocyte phenotype, in a dose-dependent manner. IOWE stimulated gene expression of C/EBP alpha (CCAAT/enhancer-binding protein alpha) and PPAR gamma (peroxisome proliferator-activated receptors gamma) during adipocyte differentiation, and induced the expression of PPAR gamma target genes such as aP2 (adipocyte protein 2), LPL (lipoprotein lipase) and CD36 (fatty acid translocase). Immunoblot analysis revealed that IOWE increased the expression of adipogenic makers such as PPAR gamma and GLUT4 (glucose transporter 4). The luciferase reporter assay demonstrated that IOWE did not exhibit PPAR gamma ligand activity. Although these results require further investigation, the ability of natural mushroom product to increase PPAR gamma transcriptional activities may be expected to be therapeutic targets for dyslipidemia and type 2 diabetes. Copyright (C) 2010 John Wiley & Sons, Ltd.