Review
Microbiology
Andrea Kunova, Cristina Pizzatti, Marco Saracchi, Matias Pasquali, Paolo Cortesi
Summary: Grapevine powdery mildew is a major fungal disease of grapevine globally, causing significant yield reductions and quality issues with intensive spraying programs leading to resistance development. There is a need for the development of effective molecular methods for monitoring and detection of resistance in biotroph pathogens.
Article
Plant Sciences
Albert K. Culbreath, Robert C. Kemerait, Timothy B. Brenneman, Emily G. Cantonwine, Keith S. Rucker
Summary: In peanut production, in-furrow applications of fluopyram + imidacloprid or fluopyram alone can effectively extend the suppression of early leaf spot and late leaf spot epidemics, serving as a complementary management tool for early-season leaf spot. The use of in-furrow applications of fluopyram should be considered as a method for resistance management as an SDHI fungicide.
Article
Chemistry, Multidisciplinary
Alba Cruz Coronel, Conrado Parraguirre Lezama, Yesenia Pacheco Hernandez, Olga Santiago Trinidad, Antonio Rivera Tapia, Omar Romero-Arenas
Summary: Strawberry wilt, caused by Fusarium solani, is a harmful disease impacting strawberry cultivation in Mexico. This study aimed to identify and characterize the strain MA-FC120 associated with root rot and wilting of strawberry crops, as well as evaluate the efficacy of four broad-spectrum fungicides against F. solani. The results showed that Benomyl 50(R) had the highest inhibition percentage, while Mancosol 80(R) and Talonil 75(R) were non-toxic to strain MA-FC120 at low concentrations.
APPLIED SCIENCES-BASEL
(2022)
Article
Agronomy
Federico Massi, Stefano Ff Torriani, Maya Waldner-Zulauf, Piero A. Bianco, Mauro Coatti, Paolo Borsa, Lorenzo Borghi, Silvia L. Toffolatti
Summary: This study assessed the baseline sensitivity of Italian Plasmopara viticola populations to oxathiapiprolin and found two insensitive strains. The results indicate a potential development of resistance in Italian P. viticola populations to oxathiapiprolin. Therefore, anti-resistance strategies should be implemented carefully, and the sensitivity levels to this molecule should be accurately monitored in the future to preserve its effectiveness.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Anshika Narang, Salvatore A. E. Marras, Natalia Kurepina, Varsha Chauhan, Elena Shashkina, Barry Kreiswirth, Mandira Varma-Basil, Christopher Vinnard, Selvakumar Subbian
Summary: The emergence of drug-resistant tuberculosis is a global health issue. This study developed a SuperSelective primer-based real-time PCR assay to efficiently diagnose heteroresistant tuberculosis, which can contribute to managing antimicrobial resistance in tuberculosis and other infectious diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiang-He Lei, Barry R. Bochner
Summary: Permeable cell models are crucial in advancing mitochondrial research, and optimizing permeabilization is needed to ensure the cell's plasma membrane allows small molecules to pass through while keeping intracellular organelles intact. The new electron flow based mitochondrial assay technology using a colorimetric redox dye provides guidance for optimizing conditions for mitochondrial function assays with permeabilized cells.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Microbiology
Electine Magoye, Lukas Nageli, Andreas Buhlmann, Maja Hilber-Bodmer, Peter Keller, Konrad Muhlethaler, Arnaud Riat, Jacques Schrenzel, Florian M. Freimoser
Summary: Fungicide applications can lead to the evolution of resistant, pathogenic fungi, and the sensitivity of environmental fungi to fungicides is mostly unknown. This study found that clinical Aureobasidium isolates were more fungicide sensitive compared to agricultural isolates, suggesting the selection for fungicide tolerance of non-target fungi in agricultural ecosystems.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agriculture, Multidisciplinary
Dalha Abdulkadir Isa, Heung Tae Kim
Summary: This study investigated the response of C. acutatum and C. gloeosporioides isolates from anthracnose infected red pepper fruits to pyraclostrobin. Molecular techniques such as cytochrome b gene sequencing, PCR-RFLP, and allele-specific PCR were used. The results showed that four isolates were resistant to pyraclostrobin and possessed a specific mutation in the cytochrome b gene. The study also observed structural differences in the cytochrome b gene between the two species. The application of molecular techniques in evaluating the resistance status provided rapid and accurate results for fungicide-resistant management strategies.
PLANT PATHOLOGY JOURNAL
(2022)
Article
Infectious Diseases
Jacob A. Tickner, Catriona S. Bradshaw, Gerald L. Murray, David M. Whiley, Emma L. Sweeney
Summary: This study developed two molecular tests to detect Mycoplasma genitalium and determine its susceptibility to fluoroquinolones. The tests showed promising results and have the potential to enhance individualized treatment and antimicrobial stewardship for M. genitalium infection.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Chemistry, Multidisciplinary
Xueqiang Wang, Weiqiang Lv, Jie Xu, Adi Zheng, Mengqi Zeng, Ke Cao, Xun Wang, Yuting Cui, Hao Li, Meng Yang, Yongping Shao, Fang Zhang, Xuan Zou, Jiangang Long, Zhihui Feng, Jiankang Liu
Summary: Alternate day fasting (ADF) leads to metabolic reprogramming in the liver and dysfunction of complex II. Knockout of the Sdhaf4 gene in the liver improves glucose tolerance and systemic insulin sensitivity, similar to the effects of ADF intervention. Hepatocytes activate the arginine-nitric oxide (NO) biosynthesis axis in response to complex II and citric acid cycle dysfunction, and the released NO enhances insulin sensitivity in muscle and adipocytes.
Article
Microbiology
J. N. Brazelton de Cardenas, C. D. Garner, Y. Su, L. Tang, R. T. Hayden
Summary: This study compared four methods for detecting antibiotic resistance genes in Enterobacterales isolates and found that commercially available PCR-based assays can accurately identify antimicrobial resistance loci in clinically significant Gram-negative bacteria, with OpGen Acuitas AMR assay showing the highest overall concordance with sequencing results.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Plant Sciences
Li Wang, Pingsheng Ji
Summary: Field isolates of P. capsici resistant to fluopicolide have been identified for the first time, showing no reduction in fitness or competitive ability compared to sensitive isolates. These findings have significant implications for growers, providing guidance on avoiding or limiting the use of this fungicide in vegetable production.
Article
Microbiology
I Gonzalez-Jimenez, R. Garcia-Rubio, S. Monzon, J. Lucio, I Cuesta, E. Mellado
Summary: Drug resistance is a global issue affecting all pathogens, with azole-resistant isolates of the human fungal pathogen Aspergillus fumigatus emerging in both clinical and environmental settings due to unintentional exposure to different antifungal drugs.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Xueqiang Wang, Xing Zhang, Ke Cao, Mengqi Zeng, Xuyang Fu, Adi Zheng, Feng Zhang, Feng Gao, Xuan Zou, Hao Li, Min Li, Weiqiang Lv, Jie Xu, Jiangang Long, Weijin Zang, Jinghai Chen, Jian Ding, Jiankang Liu, Zhihui Feng
Summary: This study reveals the importance of succinate dehydrogenase complex II assembly in the development of dilated cardiomyopathy and provides insights into potential therapeutic interventions for heart diseases.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Jiao-Sheng Li, Luo-Yu Wu, Hui Zhang, Xiu-Shi Song, Jian-Xin Wang, Ming-Guo Zhou, Yi-Ping Hou
Summary: Phenamacril is a fungicide that effectively controls wheat scab caused by Fusarium spp. Through PCR-RFLP, three common mutations in the myosin-5 gene can be detected in F. graminearum with resistance to phenamacril. This method showed a 95.12% accuracy in detecting resistant strains compared to conventional methods.
Article
Plant Sciences
Panagiota Ntasiou, Anastasios Samaras, George Karaoglanidis
Summary: This study identified the major pathogens of apple core rot in Greece and confirmed the pathogenicity of Kalmusia variispora for the first time. Field experiments showed that applying various fungicides at different times can effectively reduce the occurrence of the disease.
Article
Plant Sciences
Stefanos Testempasis, Georgia Tanou, Ioannis Minas, Martina Samiotaki, Athanassios Molassiotis, Georgios Karaoglanidis
Summary: The research showed that ozone treatment can reduce the severity of gray mold in apple fruits, while 1-MCP treatment is not effective against this disease. In the fruits, the levels of defense-related proteins decrease in fruits not treated with ozone after infection with Botrytis cinerea, while these proteins significantly increase in fruits treated with ozone.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Nazar Hussain, Emmanilo Delar, Marianne Piochon, Marie-Christine Groleau, Faiza Tebbji, Adnane Sellam, Eric Deziel, Charles Gauthier
Summary: The study reported the total synthesis of two proposed structures of gladiosides I and II derived from Burkholderia gladioli, revealing significant discrepancies between the synthetic compounds and the natural products, indicating a possible misassignment of the originally proposed structures. Additionally, an acid-catalyzed fragmentation mechanism was uncovered.
CARBOHYDRATE RESEARCH
(2021)
Article
Microbiology
Anastasios Samaras, George S. Karaoglanidis, Georgios Tzelepis
Summary: The biocontrol agent Bacillus subtilis MBI 600 has been found to inhibit mycelial growth of the plant pathogen B. cinerea in vitro and reduce disease occurrence in cucumber plants. Treatment with B. subtilis induced genes related to plant immunity, indicating its potential role in disease control.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Panagiota Ntasiou, Alexandra Kaldeli Kerou, Theodora Karamanidou, Afrodite Vlachou, George T. Tziros, Alexander Tsouknidas, George S. Karaoglanidis
Summary: The study synthesized four copper-based nanoparticles using a wet chemistry approach and characterized them using various methods. In vitro and in planta experiments showed that CuNP Type 3 and CuNP Type 4 exhibited strong protective activity against olive leaf spot and anthracnose, with significantly higher control efficacy values compared to reference products.
Article
Plant Sciences
Anastasios Samaras, Efstathios Roumeliotis, Panagiota Ntasiou, George Karaoglanidis
Summary: Bs MBI600, a recently commercialized PGPR, promotes tomato growth and shows biocontrol efficacy against soilborne pathogens. The application of Bs MBI600 increases shoot and root lengths in tomato plants, while activating auxin-related genes. Transcriptomic analysis suggests the induction of systemic resistance in tomato plants treated with Bs MBI600.
Article
Chemistry, Multidisciplinary
Natasa P. Kalogiouri, Emmanouil-Nikolaos Papadakis, Maria G. Maggalou, George S. Karaoglanidis, Victoria F. Samanidou, Urania Menkissoglu-Spiroudi
Summary: A simple and rapid analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for simultaneous determination of pesticide residues and mycotoxins in apples. Through optimization of microwave-assisted extraction, the method demonstrated superiority in extraction efficiency and rapidity compared to the QuEChERS sample preparation method.
APPLIED SCIENCES-BASEL
(2021)
Article
Genetics & Heredity
Bekris Fotios, Vasileiadis Sotirios, Papadopoulou Elena, Samaras Anastasios, Testempasis Stefanos, Gkizi Danae, Tavlaki Georgia, Tzima Aliki, Paplomatas Epaminondas, Markakis Emmanuel, Karaoglanidis George, Papadopoulou K. Kalliope, Karpouzas G. Dimitrios
Summary: The study revealed that grapevine trunk disease symptomatic plants support a wood fungal microbiome, showing cultivar and biogeography-dependent patterns that can be used as a proxy to distinguish between healthy and diseased vines. Furthermore, it highlighted strong interactions between the bacterial and fungal wood microbiome in asymptomatic vines.
ENVIRONMENTAL MICROBIOME
(2021)
Article
Environmental Sciences
Christina V. Papazlatani, Maria Kolovou, Elisabeth E. Gkounou, Konstantinos Azis, Zografina Mavriou, Stefanos Testembasis, George S. Karaoglanidis, Spyridon Ntougias, Dimitrios G. Karpouzas
Summary: The study aimed to isolate microorganisms capable of degrading the persistent fungicide IMZ and test their removal efficiency. A Cladosporium herbarum strain was successfully isolated from enrichment cultures of soil receiving effluents from FPP, showing potential for biotechnological exploitation in FPPs.
ENVIRONMENTAL POLLUTION
(2022)
Article
Food Science & Technology
Stefanos I. Testempasis, Nathalie N. Kamou, Emmanouil-Nikolaos Papadakis, Urania Menkissoglu-Spiroudi, George S. Karaoglanidis
Summary: The study investigated the impact of conventional and organic farming systems on Black Aspergilli population structure in Greek vineyards and found that farming system may affect the species composition within the vineyard, with a higher risk of selection of Aspergillus strains with higher mycotoxigenic capacity in conventional vineyards.
Article
Agronomy
George T. Tziros, George S. Karaoglanidis
Summary: In recent years, the cultivation of leafy vegetables as baby leaves has gained recognition in the global market and has attracted the interest of consumers. This study conducted a comprehensive analysis of specific Rhizoctonia solani AGs and Pythium spp. derived from baby leafy vegetables exhibiting damping-off symptoms in Greece. The results showed variations in pathogenicity among the different AGs of R. solani and identified three Pythium spp. The findings highlight the importance of understanding these pathogens in order to prevent and manage damping-off symptoms in baby leaf vegetables.
Article
Plant Sciences
Anastasios Samaras, Nathalie Kamou, Georgios Tzelepis, Katerina Karamanoli, Urania Menkissoglu-Spiroudi, George S. Karaoglanidis
Summary: In this study, a detailed transcriptomic and metabolomic analysis was conducted to investigate the response of cucumber roots to the Bs MBI600 strain. The results revealed that Bs MBI600 promotes plant growth by activating defense-related genes, inducing ISR and SAR signaling, and enhancing phytohormone production and nutrient availability. Additionally, the differentially expressed genes were found to be classified into biocontrol-related pathways.
Article
Microbiology
George T. T. Tziros, Anastasios Samaras, George S. S. Karaoglanidis
Summary: A study in Greece identified Fusarium equiseti as the pathogen responsible for an emerging foliar disease in lettuce plants. The researchers developed a highly specific RT-qPCR assay for the detection and quantification of F. equiseti in soil samples.
Article
Plant Sciences
Panagiota Ntasiou, Anastasios Samaras, Emmanouil-Nikolaos Papadakis, Urania Menkissoglu-Spiroudi, George S. S. Karaoglanidis
Summary: Penicillium expansum is a common postharvest pathogen of apple fruit, causing blue mold disease. Fungicide-resistant MDR strains with overexpression of efflux transporters have been selected. This study investigated the aggressiveness and patulin production of MDR strains, as well as the expression pattern of patulin biosynthesis pathway genes. Results showed that MDR strains produced more patulin but were less pathogenic, and the expression levels of patulin biosynthesis genes did not correlate with patulin concentration. The selection of MDR strains and their increased patulin production pose a serious concern for disease control and human health.
Article
Microbiology
Stefanos I. Testempasis, George S. Karaoglanidis
Summary: Fungicide applications can reduce fungal populations and influence pathogen evolution. This study examined the population structure of black aspergilli species in Greek vineyards and found widespread resistance to fungicides in A. uvarum isolates from conventional vineyards. However, A. tubingensis isolates showed sensitivity to pyraclostrobin. Sequencing analysis revealed mutations in the genes of resistant A. uvarum isolates, suggesting the contribution of fungicide resistance to population structure.