Article
Biotechnology & Applied Microbiology
Keun-Tae Kim, Ji-Young Oh, Seokwoo Park, Seong-Min Kim, Patterson Benjamin, In-Hyun Park, Kwang-Hoon Chun, Young-Tae Chang, Hyuk-Jin Cha
Summary: The study investigates the distinct traits between naive and primed pluripotent stem cells, revealing the high intracellular glycogen levels and requirement for oxidative phosphorylation in naive cells. Naive cells can be rescued by active oxidative phosphorylation and high levels of intracellular glycogen, providing a potential novel biomarker for metabolic remodeling during the transition of naive pluripotency.
METABOLIC ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Hester C. van Diepen, Robin A. Schoonderwoerd, Ashna Ramkisoensing, Jan A. M. Janse, Samer Hattar, Johanna H. Meijer
Summary: The study reveals the significant role of cone photoreceptors in regulating circadian rhythms, transmitting light information to the suprachiasmatic nuclei, and affecting their electrical activity levels.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Magda Correia, Francisco Santos, Rita da Silva Ferreira, Rita Ferreira, Bruno Bernardes de Jesus, Sandrina Nobrega-Pereira
Summary: Heart disease is the leading cause of mortality in developed countries, characterized by loss of cardiomyocytes leading to heart failure. Current cardiac regenerative strategies lack clinical effectiveness, partly due to the loss of regenerative capacity in postnatal stages when cardiomyocytes become terminally differentiated. Understanding metabolic determinants of cardiomyocyte function is key for future cardiac regeneration interventions.
Article
Cell Biology
Baojiang Wu, Zhiqing Yang, Yijie Liu, Jianwen Li, Chen Chen, Xihe Li, Siqin Bao
Summary: This study used a defined medium to derive ACL-ESCs and ACL-XEN cells from one blastocyst and successfully generated ACL-blastoids by combining ACL-ESCs and ACL-XEN cells with ACL-blastoid medium in a three-dimensional culture system. The lineage markers expression analysis showed that ACL-ESCs and ACL-XEN cells represented ICM and PrE lineages. This study provides a valuable ACL culture system for deriving and maintaining two distinct cell lines and also contributes to our understanding of early embryogenesis and the application of stem cells.
CELL PROLIFERATION
(2023)
Article
Cell & Tissue Engineering
Jiuru Li, Alexandra Wiesinger, Lianne Fokkert, Bastiaan J. Boukens, Arie O. Verkerk, Vincent M. Christoffels, Gerard J. J. Boink, Harsha D. Devalla
Summary: This study comprehensively characterized different subtypes of cardiomyocytes derived from human induced pluripotent stem cells (hiPSC) and established a novel three-dimensional model to study impulse initiation and propagation in the heart. Through single cell RNA sequencing and electrophysiological characterization, the study confirmed distinct cell populations with expected identities. The differences in impulse propagation speed between subtypes were more pronounced in three-dimensional cultures compared to two-dimensional monolayers, indicating a more mature phenotype. Furthermore, a binary model of pacemaker-atrial interface was established to study the interaction between cells. The findings provide valuable insights into the molecular and electrophysiological properties of hiPSC-derived cardiomyocyte subtypes and contribute to the development of composite cardiac models for drug discovery, disease modeling, and cell-based regenerative therapies.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Cell Biology
Xinghua Feng, Jian Xiong, Weijie Cai, Jin-Bin Tian, Michael X. Zhu
Summary: This study revealed that the three OcTPCs form phosphoinositide-activated Na+ channels with different ligand selectivity, voltage dependence, and extracytosolic pH sensitivity. These findings provide important clues for further research and understanding of the function and regulation of these three two-pore channels.
Article
Medicine, Research & Experimental
Xin Chen, Tianqi Liu, Jianqi Wu, Chen Zhu, Gefei Guan, Cunyi Zou, Qing Guo, Xiaolin Ren, Chen Li, Peng Cheng, Wen Cheng, Anhua Wu
Summary: This study extracted genetic and epigenetic features of TP53mut tumors and identified five different subtypes of TP53mut patients. These subtypes have distinct characteristics in terms of genomic alterations, clinical relevance, microenvironment dysregulation, and potential therapeutics. Among the subtypes, COCA3 was identified as having the worst prognosis, while olaparib showed promising therapeutic efficacy.
Article
Cell Biology
Nynke Talma, Emma Gerrits, Boshi Wang, Bart J. L. Eggen, Marco Demaria
Summary: Cells expressing high levels of the CDK4/6 inhibitor p16 accumulate in aging tissues and promote age-related pathologies, particularly neurodegeneration. This study identifies two distinct subpopulations of p16-expressing microglia in the mouse brain, one associated with aging and one present in young animals, which could impact brain homeostasis, function, and disease positively or negatively.
Article
Oncology
Haichuan Hu, Zofia Piotrowska, Patricia J. Hare, Huidong Chen, Hillary E. Mulvey, Aislinn Mayfield, Sundus Noeen, Krystina Kattermann, Max Greenberg, August Williams, Amanda K. Riley, Jarad J. Wilson, Ying-Qing Mao, Ruo-Pan Huang, Mandeep K. Banwait, Jeffrey Ho, Giovanna S. Crowther, Lida P. Hariri, Rebecca S. Heist, David P. Kodack, Luca Pinello, Alice T. Shaw, Mari Mino-Kenudson, Aaron N. Hata, Lecia Sequist, Cyril H. Benes, Matthew J. Niederst, Jeffrey A. Engelman
Summary: Research has shown that cancer-associated fibroblasts (CAFs) have three functional subtypes, with their functional differences correlating with patients' clinical response to targeted therapies and the tumor immune microenvironment, providing a new avenue for guiding personalized treatment.
Review
Cell Biology
Claudia Rejano-Gordillo, Ana Ordiales-Talavero, Ana Nacarino-Palma, Jaime M. Merino, Francisco J. Gonzalez-Rico, Pedro M. Fernandez-Salguero
Summary: The transcription factor aryl hydrocarbon receptor (AHR) is a key regulator involved in cell functions and tumor progression. It can act as either an oncogene or a tumor suppressor, depending on the specific organ, tissue and cell type. In addition to its role in cell adhesion and migration, AHR has potential as a therapeutic tool, diagnosis and prognosis marker.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Kunwei Wu, David Castellano, Qingjun Tian, Wei Lu
Summary: The study reveals distinct roles of GluN2A and GluN2B-NMDA receptors in regulating tonic inhibition in hippocampal neurons, with NMDAR subunit-specific mechanisms at play. Tonic inhibition is modulated by various factors in both physiological and pathological conditions.
Article
Genetics & Heredity
Jia Lei, Shuping Yan, Xiangqian Guo, Fengling Wang, Guosen Zhang, Quancheng Kan, Ruixia Guo
Summary: This study identified two distinct molecular subtypes of Endometrioid adenocarcinoma (EAC), with subtype I associated with a more favorable prognosis and subtype II representing high-grade EAC. The up-regulated genes in each subtype were associated with different pathways, and potential subtype-specific biomarkers were also identified.
FRONTIERS IN GENETICS
(2021)
Article
Neurosciences
Julian Wenzel, Shalaila S. Haas, Dominic B. Dwyer, Anne Ruef, Oemer Faruk Oeztuerk, Linda A. Antonucci, Sebastian von Saldern, Carolina Bonivento, Marco Garzitto, Adele Ferro, Marco Paolini, Janusch Blautzik, Stefan Borgwardt, Paolo Brambilla, Eva Meisenzahl, Raimo K. R. Salokangas, Rachel Upthegrove, Stephen J. Wood, Joseph Kambeitz, Nikolaos Koutsouleris, Lana Kambeitz-Ilankovic
Summary: By studying neurocognitive subtypes in recently onset psychosis patients, two subgroups were identified: cognitively spared and impaired. Impaired patients exhibited more severe cognitive and functional impairments compared to the spared group, suggesting a potential neurodevelopmental aspect in schizophrenia.
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Medicine, General & Internal
Fan Wu, Yi-Yun Yin, Wen-Hua Fan, You Zhai, Ming-Chen Yu, Di Wang, Chang-Qing Pan, Zheng Zhao, Guan-Zhang Li, Wei Zhanga
Summary: Through integrated analysis of transcriptome, genome, and methylome, two intrinsic molecular subtypes associated with distinct molecular characteristics and clinical outcomes were identified and validated. Oligo1 subtype was characterized by worse prognosis and enrichment of cell proliferation and Wnt signaling pathways, while Oligo2 subtype had a favorable outcome with increased activation of immune response and metabolic process. This classification serves as an independent prognostic factor in oligodendrogliomas, and its robustness was verified in validation cohorts.
Article
Multidisciplinary Sciences
Xizhao Chen, Mansheng Li, Songbiao Zhu, Yang Lu, Shuwei Duan, Xu Wang, Yong Wang, Pu Chen, Jie Wu, Di Wu, Zhe Feng, Guangyan Cai, Yunping Zhu, Haiteng Deng, Xiangmei Chen
Summary: We constructed a quantitative proteome atlas for IgA nephropathy (IgAN) and identified three subtypes based on proteomic profiles. We found differences in protein expression, subcellular injury, and extracellular accumulation among the subtypes. The complement mitochondrial extracellular (CME) pathway showed high diagnostic potential in distinguishing IgAN subtypes. Moreover, proteins related to glial cells, endothelial cells, and tubular interstitial fibrosis were highly expressed in certain subtypes and associated with worse prognosis.
