Article
Biotechnology & Applied Microbiology
Marco Vicari, Reza Mirzazadeh, Anna Nilsson, Reza Shariatgorji, Patrik Bjaerterot, Ludvig Larsson, Hower Lee, Mats Nilsson, Julia Foyer, Markus Ekvall, Paulo Czarnewski, Xiaoqun Zhang, Per Svenningsson, Lukas Kaell, Per E. Andren, Joakim Lundeberg
Summary: We present a method that combines histology, mass spectrometry imaging, and spatial transcriptomics to measure mRNA transcripts and low-molecular-weight metabolites simultaneously across tissue regions. The potential of this method is demonstrated using mouse and human brain samples in the context of dopamine and Parkinson's disease.
NATURE BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Runmin Wei, Siyuan He, Shanshan Bai, Emi Sei, Min Hu, Alastair Thompson, Ken Chen, Savitri Krishnamurthy, Nicholas E. Navin
Summary: CellTrek is a computational method that combines single-cell RNA sequencing and spatial transcriptomics to achieve single-cell spatial mapping. It has demonstrated accuracy and robustness through simulation and experimental validation, and has been successfully applied to tumor tissues.
NATURE BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Rubina Aktar, Silvia Rondinelli, Madusha Peiris
Summary: Members of the GPCR superfamily play diverse physiological roles and are valuable targets for drug development. However, the orphan receptor GPR84 remains less characterized. GPR84 is stimulated by dietary nutrients and synthetic agonists, and emerging data suggest its involvement in inflammation and energy sensing for food intake regulation. This article reviews the various roles of GPR84, particularly in the gastrointestinal tract, based on pre-clinical and clinical studies.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
Kaleb M. Naegeli, Mehmet H. Kural, Yuling Li, Juan Wang, Emmanuelle A. Hugentobler, Laura E. Niklason
Summary: Cardiovascular defects, injuries, and degenerative diseases often require surgical intervention and the use of implantable replacement materials. However, traditional grafts have limitations. Researchers are exploring avenues such as bioengineering to address these limitations.
CIRCULATION RESEARCH
(2022)
Review
Immunology
Jeremiah Bernier-Latmani, Alejandra Gonzalez-Loyola, Tatiana V. Petrova
Summary: This review discusses the molecular mechanisms of organ-specific functions of blood and lymphatic intestinal vessels, highlighting recent findings and proposing future research directions. The intestinal vasculature plays a crucial role in nutrient absorption and distribution. Recent insights have revealed the organization, molecular mechanisms, and functions of intestinal vessels, including the maintenance of intestinal epithelial cell function and recruitment of gut-specific immune cells. The review also emphasizes the systemic and cellular regulation of intestinal function, exploring its potential applications in nutrient uptake optimization and disease treatment.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Marcel A. Heinrich, Marina Mangia, Jai Prakash
Summary: This forum highlights the consequences of endotoxin contamination in biomaterials on different engineered tissues and discusses its potential effects on novel 3D immunocompetent pathophysiological models.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Cell Biology
Zhe Lin, Shuang Zhao, Xuesong Li, Zian Miao, Jiawei Cao, Yurong Chen, Zhiguang Shi, Jia Zhang, Dongjin Wang, Shaoliang Chen, Liansheng Wang, Aihua Gu, Feng Chen, Tao Yang, Kangyun Sun, Yi Han, Liping Xie, Hongshan Chen, Yong Ji
Summary: Research has discovered that post-translational modification of a protein can specifically regulate the editing of its own mRNA. The study also reveals that CTSB protein undergoes S-nitrosylation, which promotes the dephosphorylation and nuclear translocation of ADD1, resulting in A-to-I RNA editing of CTSB mRNA by MATR3 and ADAR1. This leads to increased stability and protein levels of CTSB.
Article
Cell Biology
Mingzhu Zheng, Chen Yao, Gan Ren, Kairui Mao, Hyunwoo Chung, Xi Chen, Gangqing Hu, Lei Wang, Xuemei Luan, Difeng Fang, Dar Li, Chao Zhong, Xiaoxiao Lu, Nikki Cannon, Mingxu Zhang, Avinash Bhandoola, Keji Zhao, John J. O'Shea, Jinfang Zhu
Summary: This study reveals that T cell factor 1 (TCF-1) is essential for the development of non-lymphoid tissue inducer (LTi) cells, specifically for the organogenesis of Peyer's patches (PPs). TCF-1 regulates the expression of lymphotoxin in PP LTi cells, and it may do so through promoting the expression of GATA3. Thus, the TCF-1-GATA3 axis is crucial for the development and tissue-specific functions of LTi cells.
Article
Peripheral Vascular Disease
Ting Xu, Bingyu Ji, Lingjun Li, Jiahui Lei, Meng Zhao, Miao Sun, Zhice Xu, Qinqin Gao
Summary: This study found that antenatal dexamethasone exposure has adverse effects on vascular function in adult male offspring, possibly due to increases in IP3R1 and Cav1.2 in blood vessels. These changes are mediated by epigenetic modifications (promotor histone modifications).
Article
Multidisciplinary Sciences
Yanxiang Deng, Marek Bartosovic, Sai Ma, Di Zhang, Petra Kukanja, Yang Xiao, Graham Su, Yang Liu, Xiaoyu Qin, Gorazd B. Rosoklija, Andrew J. Dwork, J. John Mann, Mina L. Xu, Stephanie Halene, Joseph E. Craft, Kam W. Leong, Maura Boldrini, Goncalo Castelo-Branco, Rong Fan
Summary: This study presents a new method for spatial chromatin accessibility profiling in tissues, allowing researchers to explore the spatial epigenetic information at the cellular level and genome scale. The technology has been successfully applied to understand gene regulators in the development of the central nervous system and immune cell types in tonsil tissue. This advancement in spatial biology improves our understanding of cell identity, cell state, and cell fate decision in relation to epigenetic underpinnings in development and disease.
Article
Biochemical Research Methods
Ian S. Kinstlinger, Gisele A. Calderon, Madison K. Royse, A. Kristen Means, Bagrat Grigoryan, Jordan S. Miller
Summary: The research describes how to fabricate and assemble customizable open-source tissue perfusion chambers, and successfully catheterize tissue constructs; endothelial cells are seeded along the luminal surfaces of the tissue constructs and connected to fluid pumping equipment; enabling various experimental designs, the protocol is expected to promote the adoption and standardization of perfusion tissue culture methods.
Review
Cell Biology
Xing-Yu Cui, Jun-Kun Zhan, You-Shuo Liu
Summary: Vascular aging and related diseases are closely associated with endothelial cells and vascular smooth muscle cells, with lncRNA playing a significant role in the pathophysiology, particularly AS-lncRNA. However, further research is needed to elucidate the specific mechanism by which ASlncRNA mediates vascular aging, with the potential to provide alternative therapeutic modalities for vascular aging-related diseases.
AGEING RESEARCH REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Christoph Holzner, Katharina Boettinger, Constantin Bloechl, Christian G. Huber, Sven O. Dahms, Elfriede Dall, Hans Brandstetter
Summary: The cysteine protease legumain can translocate to the cell surface through interaction with the RGD-dependent integrin receptor alpha V beta 3. Legumain processes the C-terminal linker region of the TrkB ectodomain, affecting TrkB-BDNF signaling. Legumain-processed TrkB still binds BDNF, suggesting a potential scavenger role of soluble TrkB towards BDNF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Xuewen Wang, Ziwei Liang, Hong Xiang, Yanqiu Li, Shuhua Chen, Hongwei Lu
Summary: LKB1 plays a crucial role in regulating macrophage functions in atherosclerosis, including influencing lipid metabolism, inflammation, endoplasmic reticulum stress, and autophagy. Decreased expression of LKB1 exacerbates vascular injury and foam cell formation in atherosclerosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Cell Biology
Emmanuel Martin, Magali Suzanne
Summary: This review discusses the role of the actin nucleation complex Arp2/3 in epithelial tissue, including its influence on cell-cell cooperation and tissue morphogenesis. It plays a significant role in regulating cell-cell adhesion, intracellular trafficking, and protrusion formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)