Article
Plant Sciences
Chuanling Li, Jian-Xiu Shang, Chenlei Qiu, Baowen Zhang, Jinxue Wang, Shuo Wang, Yu Sun
Summary: The study suggests that the EMB2726 protein plays a critical role in Arabidopsis embryogenesis by affecting chloroplast development, possibly through impacting the transcription process within plastids. The presence of undifferentiated proplastids in emb2726 embryos, along with reduced expression of certain genes, highlights the significance of this gene in embryonic development.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Daniel Hickl, David Scheuring, Torsten Moehlmann
Summary: CTPS2 plays an essential role in providing CTP supply during Arabidopsis embryo development, with its loss-of-function mutants being embryo lethal. The distinct expression pattern of CTPS2 throughout embryo development suggests a cell-type-specific regulation, and complementation with CTPS2 under its endogenous promoter rescues the observed phenotype.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Jin-Yu Li, Jing-Liang Sun, Ying-Ying Tian, Jian-Xiang Liu
Summary: Chloroplasts are crucial for plant growth and development, and their development is regulated by environmental temperature changes. However, the molecular mechanism of how plants coordinate chloroplast development and temperature changes is still largely unknown. In this study, a temperature-conditioned chloroplast development defective mutant in Arabidopsis was identified, and it was found that FtsHi5/TSL2 plays an important role in chloroplast development and protein accumulation, especially at low temperatures.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Megan Overlander-Chen, Craig H. Carlson, Jason D. Fiedler, Shengming Yang
Summary: Chloroplast biogenesis is crucial for crop biomass and yield. In this study, the regulatory machinery for chloroplast biogenesis in barley was explored through map-based cloning of the Gpa1 gene. The PTOX-encoding gene (HORVU.MOREX.r3.2HG0213170) was identified as the causal gene of Gpa1. This finding enhances our understanding of chloroplast biosynthesis and highlights the conservation of PTOX activity in monocots and dicots for photosynthesis.
Article
Plant Sciences
Weihan Fu, Zheng Cui, Jia Guo, Xiayu Cui, Guomao Han, Yunpeng Zhu, Jinju Hu, Xiaoling Gao, Yeqing Li, Min Xu, Aigen Fu, Fei Wang
Summary: Excess light can damage photosystem II (PSII) where electron transfer occurs. This study focuses on high light (HL) responsive immunophilin genes in green algae and identifies a chloroplast lumen-localized immunophilin, CYN28, as essential for HL tolerance. Lack of CYN28 leads to HL hypersensitivity, reduced accumulation of PSII supercomplexes, and compromised PSII repair. The study also reveals the role of CYN28 in regulating the turnover of thylakoid FtsH, a key protein involved in PSII repair, through its peptidyl-prolyl isomerase (PPIase) activity.
Article
Biochemistry & Molecular Biology
Jie Lan, Qibing Lin, Chunlei Zhou, Xi Liu, Rong Miao, Tengfei Ma, Yaping Chen, Changling Mou, Ruonan Jing, Miao Feng, Thanhliem Nguyen, Yulong Ren, Zhijun Cheng, Xin Zhang, Shijia Liu, Ling Jiang, Jianmin Wan
Summary: A novel P-type chloroplast-targeted PPR protein, YLWS, is found to be involved in chloroplast development during early leaf development through participating in chloroplast RNA group II intron splicing.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Cell Biology
Michal Gur, Tamir Edri, Sally A. Moody, Abraham Fainsod
Summary: Retinoic acid (RA) is a crucial signal for regulating developmental processes in vertebrate embryos. Inhibiting RA biosynthesis causes various defects in gastrulation, including delayed progression and rostral migration, as well as altered morphogenetic movements and gene expression patterns. These effects are likely due to impaired fibronectin deposition, compromised blastocoel separation, and reduced non-canonical Wnt signaling activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Mengmeng Kong, Yaozong Wu, Ziyuan Wang, Wantong Qu, Yixin Lan, Xin Chen, Yanyun Liu, Perveen Shahnaz, Zhongnan Yang, Qingbo Yu, Hualing Mi
Summary: The RP8 gene is crucial for the processing of the rpoA transcript in Arabidopsis chloroplasts, affecting gene expression and chloroplast development.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Zi-Yuan Wang, Wan-Tong Qu, Tong Mei, Nan Zhang, Nai-Ying Yang, Xiao-Feng Xu, Hai-Bo Xiong, Zhong-Nan Yang, Qing-Bo Yu
Summary: This study further analyzed the function of AtRsmD in chloroplast development and photosynthesis, revealing its distinct regulatory roles in chloroplast translation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zhipan Yang, Mingxin Liu, Shunhua Ding, Yi Zhang, Huixia Yang, Xiaogang Wen, Wei Chi, Congming Lu, Qingtao Lu
Summary: Plastid-encoded RNA polymerase (PEP)-dependent transcription is crucial for chloroplast development and plant growth. PD1 has been identified as a novel regulator for PEP-dependent gene expression and chloroplast development in Arabidopsis. PD1 interacts with PEP core subunit beta and is essential for the accumulation of PEP-beta, impacting chloroplast development in Arabidopsis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jessica A. S. Barros, Sahar Magen, Taly Lapidot-Cohen, Leah Rosental, Yariv Brotman, Wagner L. Araujo, Tamar Avin-Wittenberg
Summary: Autophagy is a conserved mechanism in eukaryotic cells that mediates the degradation of cytoplasmic components, and in plants it is associated with protein recycling during carbon-starvation conditions. The study found that autophagy plays a role in maintaining the homeostasis of multiple lipid components under dark-induced senescence, suggesting a metabolic reprogramming towards lipid storage in autophagy mutants. The imbalance of lipid metabolism in autophagy mutants compromises the production of cytosolic lipid droplets and the regulation of peroxisomal lipid oxidation pathways.
Article
Genetics & Heredity
Mei Fu, Xiaona Lin, Yining Zhou, Chunmei Zhang, Bing Liu, Dongru Feng, Jinfa Wang, Hongbin Wang, Honglei Jin
Summary: RNA editing is essential for compensating for defects or mutations in haploid organelle genomes. In this study, OTP970, a plastid-targeted PPR protein, was identified as a key player in RNA editing in plastids. The DYW domain of OTP970 was found to be essential for its function.
Article
Plant Sciences
Shujing Chen, Xinhuang Zeng, Yiqi Li, Shijun Qiu, Xiaoqun Peng, Xinjue Xie, Yujie Liu, Chancan Liao, Xiaoyan Tang, Jianxin Wu
Summary: This study investigated the functions of plant plastid ribosomal protein L18s (PRPL18s) and found that they play critical roles in plastid ribosome biogenesis, plastid intron splicing, and chloroplast development, and are essential for plant survival.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yuqin Zhang, Yuanyuan Hu, Zhiqin Wang, Xiaodi Lin, Zihui Li, Yafang Ren, Jie Zhao
Summary: This study investigates the biological functions of AtTIM22-2 in Arabidopsis. Loss of AtTIM22-2 function leads to defects in embryo and endosperm development, resulting in seed abortion. AtTIM22-2 interacts with AtTIM23-2, AtB14.7, and AT5G27395, participating in mitochondrial biogenesis and maintaining mitochondrial functions and ROS homeostasis.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Louise Chambon, Francois-Xavier Gillet, Maha Chieb, David Cobessi, Thomas Pfannschmidt, Robert Blanvillain
Summary: Chloroplast biogenesis relies on a complex transcriptional program that involves coordinated expression of plastid and nuclear genes. The researchers discovered that some plastid-encoded RNA polymerase subunits (PAPs) are present in the nucleus and may play a nuclear function in efficient chloroplast formation. They also found that PAP8 can form a large nuclear subcomplex with other subunits of the plastid-encoded RNA polymerase. Additionally, PAP8 exhibits structural homology with a rhinoviral RNA-dependent RNA polymerase. This study provides insights into the important role of PAP8 in chloroplast formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)