Article
Immunology
Christina R. DeMaso, Lovkesh Karwal, Melissa Zahralban-Steele, David Dominguez, Zhang-Li Springer, Maima Kaiser, Sunil Palani, Tim Rindfleisch, Kelly Bohning, Greg Hather, Subash Das, Mayuri Sharma, Hansi J. Dean
Summary: The study found that the TAK-003 vaccine can induce broad type-specific and cross-reactive neutralizing antibody response.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Isamu Tsuji, David Dominguez, Michael A. Egan, Hansi J. Dean
Summary: A novel avidity assay using biolayer interferometry and dengue virus-like particles was developed to assess antibody affinity maturation. Vaccination with tetravalent dengue vaccine candidate (TAK-003) increased avidity index, which remained high throughout 1 year postvaccination.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Immunology
Jue Hou, Weijian Ye, Jianzhu Chen
Summary: Dengue is a common mosquito-borne disease caused by four closely related viruses. Developing a vaccine that can protect against all four virus strains without adverse effects is a challenge. Several tetravalent live attenuated vaccine candidates have entered clinical trials.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Sitara Nasar, Zara Nasar, Saima Iftikhar
Summary: Dengue fever is a global health issue with increasing infections each year. In this study, a novel multi-epitope peptide vaccine is developed, targeting balanced immunity against dengue serotypes without severe side-effects. Immunoinformatics approaches and molecular docking studies confirm the potential of the vaccine to induce immune responses without allergic reactions.
MICROBIAL PATHOGENESIS
(2022)
Article
Immunology
Carlos A. DiazGranados, Edith Langevin, Matthew Bonaparte, Saranya Sridhar, Tifany Machabert, Gustavo Dayan, Remi Forrat, Stephen Savarino
Summary: The study showed that the tetravalent dengue vaccine is effective in both monotypic and multitypic immune profiles, with a more significant impact observed in individuals with multitypic immune profiles.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Ramil R. Mintaev, Dina V. Glazkova, Olga V. Orlova, Georgiy M. Ignatyev, Alexey S. Oksanich, German A. Shipulin, Elena V. Bogoslovskaya
Summary: Dengue fever is a global health problem, affecting over 100 million people annually. Vaccination is the most effective prevention strategy, but the development of vaccines is hindered by the risk of antibody-dependent enhancement. This article presents the development of an MVA-d34 vaccine against dengue virus, using safe and effective MVA viral vector. The vaccine targets the DIII domains of the envelope protein, which do not cause enhanced infection. Immunized mice generate a humoral response against all four dengue virus serotypes, and their sera have virus-neutralizing activity against dengue serotype 2. The MVA-d34 vaccine shows promise as a candidate vaccine against dengue fever.
Article
Infectious Diseases
Laura J. White, Ellen F. Young, Mark J. Stoops, Sandra R. Henein, Elizabeth C. Adams, Ralph S. Baric, Aravinda M. de Silva
Summary: The four serotypes of dengue virus infect millions of people each year, making the development of vaccines challenging. TAK-003, a tetravalent live attenuated dengue vaccine candidate, induces high levels of neutralizing antibodies. The vaccine primarily generates neutralizing antibodies targeting unique epitopes on DENV2, while the levels of neutralizing antibodies against DENV1, DENV3, and DENV4 are lower.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Immunology
Daniela Michlmayr, Paulina Andrade, Eduardo J. M. Nascimento, Allan Parker, Parnal Narvekar, Hansi J. Dean, Eva Harris
Summary: This study analyzed the specific and cross-reactive memory B cell responses to dengue virus in individuals who received a tetravalent vaccine candidate. The results showed that tetravalent vaccination induced type-specific memory B cells, demonstrating a significant contribution from all 4 components of the vaccine to the DENV-specific memory B cell response.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Ziting Ma, Jinnian Guo, Lu Jiang, Suqing Zhao
Summary: Dengue is a common and deadly mosquito-borne infectious disease globally. Early detection of dengue virus is crucial for preventing disease spread and providing timely response. Lateral flow immunoassay (LFIA) systems are low-cost, rapid, sensitive, and simple detection methods suitable for point-of-care testing in dengue-affected areas. However, current commercial LFIA kits fail to fully meet the requirements for sensitivity, specificity, serotype differentiation, and multiplex detection. Various strategies have been applied to optimize LFIA for dengue detection.
Editorial Material
Multidisciplinary Sciences
Scott B. Biering, Eva Harris
Summary: The compound JNJ-A07 shows promising activity against the dengue virus in mouse models, offering hope for a treatment for this prevalent mosquito-borne viral disease in humans.
Article
Immunology
Amina Basheer, Syed Babar Jamal, Badr Alzahrani, Muhammad Faheem
Summary: Dengue fever is a mosquito-borne disease that affects approximately 3.9 billion people globally each year. Currently, there is no available vaccine or drug for dengue virus infection. In this study, researchers designed a tetravalent subunit multi-epitope vaccine targeting different proteins from each serotype. The vaccine has shown potential to elicit a strong immune response against all dengue serotypes without causing any adverse effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Peifang Sun, Vihasi Jani, Alison Johnson, Ying Cheng, Nishith Nagabhushana, Maya Williams, Brian J. Morrison, Gabriel Defang
Summary: TLAV vaccination enhances innate immune gene expression, provides better protection against dengue virus, and more effectively boosts T cell IFN gamma response compared to TDNA and TPIV vaccines. TPIV vaccination alone induces higher B-Mem and weaker IFN gamma response, but significantly enhances T cell response when boosted with TLAV vaccination.
Article
Biophysics
Qi Yan Siew, Ee Leen Pang, Hwei-San Loh, Michelle T. T. Tan
Summary: This study presents a novel impedimetric immunosensor design using plant-derived antigenic glycoprotein for the detection of dengue virus (DENV) IgG antibodies, showing high sensitivity and specificity. The developed immunosensor is proposed as a reliable and practical diagnostic tool for serological detection of dengue in realistic applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Public, Environmental & Occupational Health
Xavier Saez-Llorens, Shibadas Biswal, Charissa Borja-Tabora, LakKumar Fernando, Mengya Liu, Derek Wallace, Nicolas Folschweiller, Humberto Reynales, Inge LeFevre
Summary: In the phase 3 trial of the TAK-003 dengue vaccine, recipients of the vaccine had a lower risk of subsequent episodes of symptomatic dengue compared to placebo recipients, indicating the vaccine may have additional effects beyond preventing the first episode.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2023)
Article
Immunology
Kirill Nemirov, Pierre Authie, Philippe Souque, Fanny Moncoq, Amandine Noirat, Catherine Blanc, Maryline Bourgine, Laleh Majlessi, Pierre Charneau
Summary: Dengue virus (DENV) is a major cause of dengue fever, including severe forms such as hemorrhagic dengue and dengue shock syndrome. Despite intensive vaccine research, a universally accepted vaccine against dengue fever has not been developed yet. This study developed a tetravalent T-cell vaccine candidate against DENV, which showed significant protective effects against all four DENV serotypes in a preclinical infection model.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.