Article
Multidisciplinary Sciences
Alyssa Miller, Jiapeng Wei, Sarah Meehan, Christopher M. Dobson, Mark E. Welland, David Klenerman, Michele Vendruscolo, Francesco Simone Ruggeri, Tuomas P. J. Knowles
Summary: Neurodegenerative diseases such as Alzheimer's disease are caused by protein misfolding and aggregation into amyloid fibrils. This study uses atomic force microscopy and statistical theory to characterize amyloid ring structures derived from the brains of AD patients and explains the diversity in the structures formed from protein aggregation. The results show that ex vivo protofibril chains possess greater flexibility than mature amyloid fibrils, allowing them to form end-to-end connections and shedding light on their role in disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Analytical
Paolo Pellegrino, Isabella Farella, Mariafrancesca Cascione, Valeria De Matteis, Alessandro Paolo Bramanti, Lorenzo Vincenti, Antonio Della Torre, Fabio Quaranta, Rosaria Rinaldi
Summary: In recent years, mechanical tip-based nanolithography technique has been developed to meet the growing demand for nanostructures. However, the resulting pile-ups of nanostructures limit their use and integration. This study characterizes the structure of pile-ups at the edges of nanogrooves and proposes an effective strategy to remove them.
Article
Multidisciplinary Sciences
Ryusei Yamada, Tran Ngoc Trang, Holger Flechsig, Toshiki Takeda, Noriyuki Kodera, Hiroki Konno
Summary: The N-terminal of annexin V has significant influence on 2D crystal formation, as observed using high-speed atomic force microscopy (HS-AFM). Furthermore, a quick purification method is presented to purify recombinant annexin V without any residual affinity tag after protein purification in approximately 3 hours.
Article
Nanoscience & Nanotechnology
Keivan Asadi, Junghoon Yeom, Hanna Cho
Summary: Investigating internal resonance (IR) mechanisms in micro/nanoresonators reveals that intermodal coupling between second and third flexural modes in asymmetric structures provides an optimal condition for strong IR, with high energy transfer to the resonated mode. This study introduces design strategies that can be easily integrated into typical micro/nanoelectromechanical systems, offering potential for paradigm-shifting applications in micro/nanosystems.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Stefania A. Mari, Kristyna Pluhackova, Joka Pipercevic, Matthew Leipner, Sebastian Hiller, Andreas Engel, Daniel J. Mueller
Summary: Gasdermin-A3 pore formation propagates along diverse pathways, and the assembly and pore-forming mechanisms have been characterized using high-resolution time-lapse atomic force microscopy. The results reveal the role of amphiphilic beta-hairpins and structurally adapting hydrophilic head domains in stabilizing variable oligomeric conformations and opening the pore.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Haruhiko Eki, Katsuhiko Abe, Hiroshi Sugiyama, Masayuki Endo
Summary: This study used atomic force microscopy to directly observe the formation and degradation of tensegrity triangle DNA crystals at a molecular level, visualizing the dynamic process and characterizing them with nanoscale resolution.
CHEMICAL COMMUNICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Xiaobin Liang, Takashi Kojima, Makiko Ito, Naoya Amino, Haonan Liu, Masataka Koishi, Ken Nakajima
Summary: In situ atomic force microscopy (AFM) nanomechanical technique was used to directly visualize the micromechanical behaviors of polymer nanocomposites during compressive strain. The stress distribution image of carbon black (CB)-filled rubber at the nanoscale was obtained for the first time, revealing the microscopic reinforcement mechanisms and heterogeneous deformation behaviors. The stress concentrations near the CB particles formed a network structure which acted as the key reinforcement mechanism. The finite-element method (FEM) simulations reproduced the heterogeneity in stress distribution, confirming the insights provided by the AFM experiments.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Editorial Material
Multidisciplinary Sciences
Telmo O. Paiva, Albertus Viljoen, Yves F. Dufrene
Summary: Advancements in atomic force microscopy (AFM) techniques and methodologies in microbiology have enhanced our understanding of microbial cell surfaces. Recent studies have shown that AFM imaging of cells and membranes at or near molecular resolution enables detailed visualization of membrane-drug interactions.
NATURE COMMUNICATIONS
(2022)
Article
Physics, Multidisciplinary
Simone Benaglia, Manuel R. Uhlig, Jose Hernandez-Munoz, Enrique Chacon, Pedro Tarazona, Ricardo Garcia
Summary: This study demonstrates that the generation of 3D maps is dependent on the polarization of the tip, with differently charged tips revealing different density distributions. Experimental data reproduce key findings of the theory.
PHYSICAL REVIEW LETTERS
(2021)
Article
Biophysics
Melvin Wei Shern Goh, Ryugo Tero
Summary: This study investigated the effects of PE unsaturation and double bond distribution on the formation of submicron domains in lipid bilayers. The results showed that submicron domains enriched with polyunsaturated PE increased in area fraction with higher PE concentration and unsaturation levels. The segregation of polyunsaturated PE from Chol-containing regions played a key role in the formation of these domains.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Da Yeon Cheong, Wonseok Lee, Insu Park, Jinsung Park, Gyudo Lee
Summary: In this study, the nucleation and formation process of lysozyme amyloid fibrils were observed using a microfluidic system. It was found that the lag phase of amyloid nucleation and growth can be reduced by using smaller droplet volumes. Furthermore, a peculiar phenomenon of high amyloid formation at the edge of droplets was discovered, and it was observed that fibrils synthesized in nanoliter droplets are shorter and thicker compared to those synthesized via conventional heating method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Jonas Heggemann, Yashasvi S. Ranawat, Ondrej Krejci, Adam S. Foster, Philipp Rahe
Summary: This study reveals the microscopic geometry of calcite(104) and the decisive impact of the (2 x 1) reconstruction on adsorbed species such as carbon monoxide, using high-resolution atomic force microscopy data combined with density functional theory and AFM image calculations.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Cell Biology
Elma Sakinatus Sajidah, Keesiang Lim, Tomoyoshi Yamano, Goro Nishide, Yujia Qiu, Takeshi Yoshida, Hanbo Wang, Akiko Kobayashi, Masaharu Hazawa, Firli R. P. Dewi, Rikinari Hanayama, Toshio Ando, Richard W. Wong
Summary: Small extracellular vesicles (sEVs) play a crucial role in cell communication and can be used for drug delivery and structural characterization of nanoparticles. The high-speed atomic force microscopy (HS-AFM) can evaluate the structural changes of sEVs under different physicochemical stresses.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Marta Z. Pacia, Natalia Chorazy, Magdalena Sternak, Benedikt Fels, Michal Pacia, Mariusz Kepczynski, Kristina Kusche-Vihrog, Stefan Chlopicki
Summary: In this study, the role of Rac1 in TNF-induced vascular inflammation was investigated. It was found that Rac1 mediated the formation of lipid droplets and nanostructural alterations associated with vascular inflammation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Engineering, Multidisciplinary
Jingyu Wang, Yong Qian, Yijie Zhou, Dongjie Yang, Xueqing Qiu
Summary: This study quantitatively investigated the dispersion mechanism of lignosulfonate/silica system using atomic force microscopy (AFM), revealing strong steric repulsion provided by lignosulfonate between SiO2 particles. Further analysis identified adsorbance, normalized interaction constant, and characteristic length as critical factors affecting steric repulsion.
Article
Chemistry, Multidisciplinary
David Z. Gao, Filippo Federici Canova, Matthew B. Watkins, Alexander L. Shluger
JOURNAL OF COMPUTATIONAL CHEMISTRY
(2015)
Article
Engineering, Electrical & Electronic
Al-Moatasem El-Sayed, Matthew B. Watkins, Tibor Grasser, Valeri V. Afanas'ev, Alexander L. Shluger
MICROELECTRONIC ENGINEERING
(2015)
Article
Chemistry, Physical
Julian Gaberle, David Z. Gao, Matthew B. Watkins, Alexander L. Shluger
JOURNAL OF PHYSICAL CHEMISTRY C
(2016)
Article
Chemistry, Multidisciplinary
Keisuke Miyazawa, Naritaka Kobayashi, Matthew Watkins, Alexander L. Shluger, Ken-ichi Amano, Takeshi Fukuma
Article
Physics, Multidisciplinary
C. M. Yim, M. B. Watkins, M. J. Wolf, C. L. Pang, K. Hermansson, G. Thornton
PHYSICAL REVIEW LETTERS
(2016)
Article
Nanoscience & Nanotechnology
K. Miyazawa, M. Watkins, A. L. Shluger, T. Fukuma
Article
Chemistry, Physical
T. R. Durrant, S. T. Murphy, M. B. Watkins, A. L. Shluger
JOURNAL OF CHEMICAL PHYSICS
(2018)
Article
Chemistry, Physical
A. R. Elmaslmane, M. B. Watkins, K. P. McKenna
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2018)
Article
Chemistry, Multidisciplinary
Franck Para, Franck Bocquet, Laurent Nony, Christian Loppacher, Michel Feron, Frederic Cherioux, David Z. Gao, Filippo Federici Canova, Matthew B. Watkins
Article
Chemistry, Physical
Jack Strand, Sergey K. Chulkov, Matthew B. Watkins, Alexander L. Shluger
JOURNAL OF CHEMICAL PHYSICS
(2019)
Article
Chemistry, Multidisciplinary
Emiliano Poli, Joshua D. Elliott, Sergey K. Chulkov, Matthew B. Watkins, Gilberto Teobaldi
FRONTIERS IN CHEMISTRY
(2019)
Correction
Chemistry, Physical
Sanliang Ling, Matthew B. Watkins, Alexander L. Shluger
JOURNAL OF PHYSICAL CHEMISTRY C
(2020)
Article
Chemistry, Physical
Anna-Sophia Hehn, Beliz Sertcan, Fabian Belleflamme, Sergey K. Chulkov, Matthew B. Watkins, Juerg Hutter
Summary: Time-dependent density functional theory has become the state-of-the-art method for describing photophysical and photochemical processes in extended materials due to its affordability. However, the treatment of exact exchange is not efficient, leading to the proposal of two approximate schemes for improving computational efficiency.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2022)
Article
Crystallography
Christopher Dickens, Adam O. J. Kinsella, Matt Watkins, Matthew Booth
Summary: This study investigates Fe substitution in CuAlS2 using density functional theory and reveals defect complexes associated with the optical resonance in CuFeS2. This is important for understanding the properties of CuFeS2 and controlling the optical properties of similar systems.
Article
Materials Science, Multidisciplinary
Al-Moatasem El-Sayed, Matthew B. Watkins, Tibor Grasser, Alexander L. Shluger