期刊
VETERINARY PARASITOLOGY
卷 212, 期 3-4, 页码 393-395出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2015.07.024
关键词
Toxoplasma gondii; Horses; Romania; Seroprevalence; Bioassay
资金
- European Social Fund, Human Resources Development Operational Programme [POSDRU/159/1.5/S/136893]
- Executive Unit for Higher Education, Research, Development and Innovation Funding (UEFISCDI, Romania) [620/2013]
The prevalence of Toxoplasmn gondii, an economically important zoonotic protozoan, was investigated in horses slaughtered for export and human consumption in the North of Romania. Pairs of samples, sera and heart tissues, were collected from 82 slaughtered horses. Examination of horse sera by ELISA at a dilution of 1:10, and by modified agglutination test (MAT) at a dilution of 1:6, revealed that 32 (39%) and 31(37.8%) horses, respectively, had antibodies against T. gondii. Using polymerase chain reaction (PCR) analysis, T. gondii DNA was not found in any heart sample collected from horses. By bioassay in mice, we obtained viable isolates of T. gondii from two of ten horses determined to be strongly positive by serological assay/ELISA. The prevalence estimated in horses highlighted the potential risk for human contamination by consumption of raw or undercooked meat. (C) 2015 Elsevier B.V. All rights reserved.
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